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The map label for this gene is msbB [H]

Identifier: 209397852

GI number: 209397852

Start: 2447347

End: 2448318

Strand: Reverse

Name: msbB [H]

Synonym: ECH74115_2591

Alternate gene names: 209397852

Gene position: 2448318-2447347 (Counterclockwise)

Preceding gene: 209396115

Following gene: 209399722

Centisome position: 43.94

GC content: 51.44

Gene sequence:

>972_bases
ATGGAAACGAAAAAAAATAATAGCGAATACATTCCTGAGTTTGATAAATCCTTTCGCCACCCGCGCTACTGGGGAGCATG
GCTGGGCGTAGCAGCGATGGCGGGTATCGCTTTAACGCCGCCAAAGTTCCGTGATCCCATTCTGGCACGGCTGGGACGTA
TTGCCGGACGACTGGGAAAAAGCTCACGCCGTCGTGCGTTAATCAATCTGTCGCTCTGCTTTCCAGAACGTAGTGAAGCT
GAACGCGAAGCGATTGTTGATGAGATGTTTGCCACCGCGCCGCAAGCGATGGCAATGATGGCTGAGTTGGCAATACGCGG
GCCGGAGAAAATTCAGCCGCGCGTTGACTGGCAAGGGCTGGAGATCATCGAAGAGATGCGGCGTAATAACGAGAAAGTTA
TCTTTCTAGTGCCGCACGGTTGGGCCGTCGATATTCCTGCCATGCTGATGGCCTCGCAAGGGCAGAAAATGGCAGCGATG
TTCCATAATCAGGGCAACCCGGTTTTTGATTATGTCTGGAACACGGTGCGTCGTCGCTTTGGCGGTCGTCTGCATGCGAG
AAATGACGGTATTAAACCATTCATCCAGTCGGTACGTCAGGGGTACTGGGGATATTATTTACCCGATCAGGATCATGGCC
CAGAGCACAGCGAATTTGTGGATTTCTTTGCCACCTATAAAGCGACGTTGCCCGCGATTGGTCGTTTGATGAAAGTGTGC
CGTGCGCGCGTTGTACCGCTGTTTCCGATTTATGATGGCAAGACGCATCGTCTGACGATTCAGGTGCGCCCACCGATGGA
TGATCTGTTAGAGGCGGATGATCATACGATTGCGCGGCGGATGAATGAAGAAGTCGAGATTTTTGTTGGTCCGCGACCAG
AACAATACACCTGGATACTAAAATTGCTGAAAACTCGCAAACCGGGCGAAATCCAGCCGTATAAGCGCAAAGATCTTTAT
CCCATCAAATAA

Upstream 100 bases:

>100_bases
GGTACGCAGTCAGTACCGGCTTTTTTTATTTGGTGCGGGGCAAGTTGCGCCGCTACACTATCACCAGATTGATTTTTGCC
TTATCCGAAACTGGAAAAGC

Downstream 100 bases:

>100_bases
AAAAGCCTCTCGCGAGGAGAGGCCTTCGCCTGATGATAAGTTCAAGTTTGCTTCAGAATATTCGAAATCTGTTGAACTAT
CATTGAACTGTAGGCCGGAT

Product: lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFRDPILARLGRIAGRLGKSSRRRALINLSLCFPERSEA
EREAIVDEMFATAPQAMAMMAELAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSEFVDFFATYKATLPAIGRLMKVC
RARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHTIARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLY
PIK

Sequences:

>Translated_323_residues
METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFRDPILARLGRIAGRLGKSSRRRALINLSLCFPERSEA
EREAIVDEMFATAPQAMAMMAELAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSEFVDFFATYKATLPAIGRLMKVC
RARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHTIARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLY
PIK
>Mature_323_residues
METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFRDPILARLGRIAGRLGKSSRRRALINLSLCFPERSEA
EREAIVDEMFATAPQAMAMMAELAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSEFVDFFATYKATLPAIGRLMKVC
RARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHTIARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLY
PIK

Specific function: Transfers myristate or laurate, activated on ACP, to (KDO)2-(lauroyl)-lipid IVA [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1788161, Length=323, Percent_Identity=99.6904024767802, Blast_Score=667, Evalue=0.0,
Organism=Escherichia coli, GI1787292, Length=310, Percent_Identity=30.6451612903226, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI87082094, Length=307, Percent_Identity=26.0586319218241, Blast_Score=106, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011921 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 37377; Mature: 37377

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFRDPILARLGRIAGRLGK
CCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCC
SSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAELAIRGPEKIQPRVDWQGL
CCCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHCCCCCCCCHH
EIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAMFHNQGNPVFDYVWNTVRRRF
HHHHHHHCCCCEEEEEECCCCEECHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHH
GGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSEFVDFFATYKATLPAIGRLMKVC
CCEEECCCCCCHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHTIARRMNEEVEIFVGPRPEQYTWIL
HHHCCEEEEEECCCEEEEEEEECCCHHHHHCCCCHHHHHHCCCCEEEEECCCCCHHHHHH
KLLKTRKPGEIQPYKRKDLYPIK
HHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFRDPILARLGRIAGRLGK
CCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCC
SSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAELAIRGPEKIQPRVDWQGL
CCCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHCCCCCCCCHH
EIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAMFHNQGNPVFDYVWNTVRRRF
HHHHHHHCCCCEEEEEECCCCEECHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHH
GGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSEFVDFFATYKATLPAIGRLMKVC
CCEEECCCCCCHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHTIARRMNEEVEIFVGPRPEQYTWIL
HHHCCEEEEEECCCEEEEEEEECCCHHHHHCCCCHHHHHHCCCCEEEEECCCCCHHHHHH
KLLKTRKPGEIQPYKRKDLYPIK
HHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12384590; 12704152 [H]