| Definition | Escherichia coli O157:H7 str. EC4115, complete genome. |
|---|---|
| Accession | NC_011353 |
| Length | 5,572,075 |
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The map label for this gene is fumA [H]
Identifier: 209397842
GI number: 209397842
Start: 2192827
End: 2194473
Strand: Reverse
Name: fumA [H]
Synonym: ECH74115_2322
Alternate gene names: 209397842
Gene position: 2194473-2192827 (Counterclockwise)
Preceding gene: 209396395
Following gene: 209396648
Centisome position: 39.38
GC content: 51.37
Gene sequence:
>1647_bases ATGTCAAACAAACCCTTTCATTATCAGGCTCCTTTTCCACTCAAAAAAGATGATACTGAGTATTACCTGCTAACCAGCGA ACACGTTAGCTTATCTGAATTTGAAGGGCAGGAGATTTTGAAAGTCGCACCCGAAGCGTTAACTCTGTTGGCGCGCCAGG CGTTTCATGATGCGTCGTTCATGCTGCGTCCGGCGCACCAACAACAGGTGGCCGACATTCTGCGTGACCCGGAGGCCAGC GAAAATGATAAATATGTGGCGCTGCAATTCCTGCGTAACTCCGACATCGCGGCGAAAGGCGTTCTGCCAACCTGTCAGGA TACCGGCACCGCGATTATTGTTGGTAAAAAAGGGCAGCGTGTATGGACCGGTGGTGGTGATGAAGCGGCGCTGGCGCGCG GTGTCTATAACACTTATATCGAAGATAATCTGCGCTACTCGCAAAACGCGCCGCTGGATATGTATAAAGAAGTGAATACC GGCACCAATCTGCCAGCGCAGATCGATCTTTATGCCGTTGATGGCGACGAATACAAATTCCTCTGTATCGCCAAAGGTGG TGGTTCGGCAAACAAGACGTATCTCTATCAGGAAACCAAAGCGTTACTGACGCCGGGGAAACTGAAAAATTACCTGGTTG AGAAGATGCGCACGCTGGGTACGGCGGCCTGTCCTCCGTATCATATTGCGTTCGTTATTGGTGGAACTTCTGCAGAAACG AACCTTAAAACGGTGAAACTGGCTTCCGCGAAATACTATGATGAACTGCCAACGGAAGGGAATGAGCACGGTCAGGCGTT CCGCGATGTGGAATTGGAAAAAGAATTGCTGATCGAAGCGCAAAATCTTGGTCTGGGTGCGCAGTTTGGCGGTAAATACT TCGCTCACGACATCCGCGTGATTCGCCTGCCACGTCACGGCGCATCCTGCCCGGTCGGTATGGGCGTCTCCTGTTCTGCT GACCGTAATATCAAAGCGAAGATCAACCGTCAGGGGATCTGGATCGAAAAACTGGAACATAATCCAGGCAAATATATCCC GGAAGAGCTGCGCAAAGCGGGAGAAGGCGAAGCGGTGCGCGTTGACCTTAACCGTCCGATGAAAGAGATCCTCGCACAGT TGTCGCAGTATCCCGTTTCTACACGCTTATCGCTTAACGGCACGATTATCGTCGGTCGTGATATTGCTCACGCCAAACTG AAAGAGCGGATGGATAACGGTGAAGGGCTGCCGCAGTACATCAAAGATCATCCGATTTACTACGCGGGTCCGGCCAAAAC GCCGGAAGGTTATGCCTCCGGTTCTCTTGGCCCAACGACCGCCGGACGGATGGATTCTTATGTCGATCAACTGCAAGCGC TGGGCGGAAGTATGATCATGCTGGCGAAAGGCAACCGCAGCCAGCAGGTGACGGATGCCTGTAAAAAACACGGTGGCTTC TACCTTGGCAGTATCGGTGGTCCGGCCGCTGTATTGGCGCAGGGAAGTATTAAGAGCCTGGAATGTGTTGAATATCCGGA ACTGGGAATGGAAGCCATCTGGAAAATTGAAGTGGAAGATTTCCCGGCGTTTATCCTTGTGGATGATAAAGGAAATGACT TCTTCCAGCAGATACAACTCACACAATGCACCCGCTGTGTGAAATAA
Upstream 100 bases:
>100_bases TTTTGTTAAAAAAGTGCGTAGGATATTGTTACTCGCTTTTAACAGGGCAACGGAACACCCGCCCAGAGCATAACCAAACC AGGCAGTAAGTGAGAAAACA
Downstream 100 bases:
>100_bases ACAGAGCCGCCCTTCGGGGCGGTTTTTTTACATGGCACGAAAGACCAAACATTTGTTATCAAATAGTAAATAATGAGTGA GCTAAAAGTTACTTAACGAA
Product: fumarate hydratase
Products: NA
Alternate protein names: Fumarase [H]
Number of amino acids: Translated: 548; Mature: 547
Protein sequence:
>548_residues MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSLSEFEGQEILKVAPEALTLLARQAFHDASFMLRPAHQQQVADILRDPEAS ENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAET NLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKELLIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSA DRNIKAKINRQGIWIEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVGRDIAHAKL KERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQALGGSMIMLAKGNRSQQVTDACKKHGGF YLGSIGGPAAVLAQGSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK
Sequences:
>Translated_548_residues MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSLSEFEGQEILKVAPEALTLLARQAFHDASFMLRPAHQQQVADILRDPEAS ENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAET NLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKELLIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSA DRNIKAKINRQGIWIEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVGRDIAHAKL KERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQALGGSMIMLAKGNRSQQVTDACKKHGGF YLGSIGGPAAVLAQGSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK >Mature_547_residues SNKPFHYQAPFPLKKDDTEYYLLTSEHVSLSEFEGQEILKVAPEALTLLARQAFHDASFMLRPAHQQQVADILRDPEASE NDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNTG TNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAETN LKTVKLASAKYYDELPTEGNEHGQAFRDVELEKELLIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSAD RNIKAKINRQGIWIEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVGRDIAHAKLK ERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQALGGSMIMLAKGNRSQQVTDACKKHGGFY LGSIGGPAAVLAQGSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK
Specific function: It functions as an aerobic enzyme in the citric acid cycle. It accounts for about 80% of the fumarase activity when the bacteria grows aerobically [H]
COG id: COG1951
COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the class-I fumarase family [H]
Homologues:
Organism=Escherichia coli, GI1787897, Length=548, Percent_Identity=99.6350364963504, Blast_Score=1133, Evalue=0.0, Organism=Escherichia coli, GI1790564, Length=548, Percent_Identity=89.7810218978102, Blast_Score=1026, Evalue=0.0, Organism=Escherichia coli, GI1789443, Length=181, Percent_Identity=28.1767955801105, Blast_Score=75, Evalue=1e-14,
Paralogues:
None
Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR004646 - InterPro: IPR004647 - InterPro: IPR011167 - InterPro: IPR020557 [H]
Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]
EC number: =4.2.1.2 [H]
Molecular weight: Translated: 60298; Mature: 60167
Theoretical pI: Translated: 6.52; Mature: 6.52
Prosite motif: PS00163 FUMARATE_LYASES
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.6 %Cys (Translated Protein) 2.0 %Met (Translated Protein) 3.6 %Cys+Met (Translated Protein) 1.6 %Cys (Mature Protein) 1.8 %Met (Mature Protein) 3.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSLSEFEGQEILKVAPEALTLLARQAFHDASF CCCCCEEEECCCCCCCCCCCEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCHH MLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQR EECCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCE VWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNTGTNLPAQIDLYAVDGDEYKF EEECCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEEECCCCEEE LCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAET EEEECCCCCCCCEEEEECHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCC NLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKELLIEAQNLGLGAQFGGKYFAHDIRV CEEEEEEECHHHHHCCCCCCCCCCCHHHHHHHHHHHHEEHHCCCCCCCCCCCEEEECEEE IRLPRHGASCPVGMGVSCSADRNIKAKINRQGIWIEKLEHNPGKYIPEELRKAGEGEAVR EEECCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEEEECCCCCCCCHHHHHCCCCCEEE VDLNRPMKEILAQLSQYPVSTRLSLNGTIIVGRDIAHAKLKERMDNGEGLPQYIKDHPIY EECCCCHHHHHHHHHHCCCEEEEEECEEEEEECCHHHHHHHHHHCCCCCCHHHHHCCCEE YAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQALGGSMIMLAKGNRSQQVTDACKKHGGF EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCE YLGSIGGPAAVLAQGSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQL EEECCCCCEEEEECCCCCCEEECCCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHH TQCTRCVK HHHHHHCC >Mature Secondary Structure SNKPFHYQAPFPLKKDDTEYYLLTSEHVSLSEFEGQEILKVAPEALTLLARQAFHDASF CCCCEEEECCCCCCCCCCCEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCHH MLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQR EECCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCE VWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNTGTNLPAQIDLYAVDGDEYKF EEECCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEEECCCCEEE LCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAET EEEECCCCCCCCEEEEECHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCC NLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKELLIEAQNLGLGAQFGGKYFAHDIRV CEEEEEEECHHHHHCCCCCCCCCCCHHHHHHHHHHHHEEHHCCCCCCCCCCCEEEECEEE IRLPRHGASCPVGMGVSCSADRNIKAKINRQGIWIEKLEHNPGKYIPEELRKAGEGEAVR EEECCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEEEECCCCCCCCHHHHHCCCCCEEE VDLNRPMKEILAQLSQYPVSTRLSLNGTIIVGRDIAHAKLKERMDNGEGLPQYIKDHPIY EECCCCHHHHHHHHHHCCCEEEEEECEEEEEECCHHHHHHHHHHCCCCCCHHHHHCCCEE YAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQALGGSMIMLAKGNRSQQVTDACKKHGGF EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCE YLGSIGGPAAVLAQGSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQL EEECCCCCEEEEECCCCCCEEECCCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHH TQCTRCVK HHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11677609; 1879695 [H]