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The map label for this gene is proA [H]

Identifier: 209397818

GI number: 209397818

Start: 301175

End: 302428

Strand: Direct

Name: proA [H]

Synonym: ECH74115_0288

Alternate gene names: 209397818

Gene position: 301175-302428 (Clockwise)

Preceding gene: 209398531

Following gene: 209397753

Centisome position: 5.41

GC content: 54.7

Gene sequence:

>1254_bases
ATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTCGTATAAATTAGCGCAACTCTCCAGCCGCGAAAAAAATCGCGT
GCTGGAAAAAATCGCCGATGAACTGGAAGCACAAAGCGAAATCATCCTCAACGCTAACGCCCAGGATGTTGCTGACGCGC
GAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGACGCCCGCACGGCTGAAAGGCATTGCCGACGATGTA
CGTCAGGTGTGCAACCTCGCCGATCCAGTGGGGCAGGTAATCGATGGCGGCGTACTGGACAGCGGCCTGCGTCTTGAGCG
TCGTCGCGTACCGCTGGGGGTTATTGGCGTGATTTATGAAGCGCGCCCGAACGTGACGGTTGATGTCGCTTCGCTGTGCC
TGAAAACCGGTAACGCGGTGATCCTGCGTGGTGGCAAAGAAACCTGTCGCACTAACGCGGCAACGGTGGTGGTGATTCAG
GACGCCCTGAAATCCTGTGGCTTACCGGCGGGTGCCGTGCAGGCGATTGATAATCCTGACCGTGCGCTGGTCAGTGAAAT
GCTGCGTATGGATAAATACATCGACATGCTGATCCCGCGCGGCGGGGCTGGTTTGCATAAACTGTGCCGCGAGCAGTCGA
CGATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTATGTTGATGAAAGTGCAGAGATTGCTGAAGCCCTGAAA
GTAATCGTCAATGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTAGAAACGTTGCTGGTGAATAAAAACATCGCCTA
TAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACGTTACACGCAGATGCATCTGCGCTGGCGCAGT
TGCAGACAGGCCCTGCGAAGGTGGTGGCGGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTC
AAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACGCAACACTCCGATGCGATCCTGACCCG
CGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCG
ACGGCGGCCAGTTTGGACTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCACGCGCGTGGCCCAATGGGGCTGGAA
GCACTGACCACTTACAAGTGGATCGGCATTGGTGATTACACCATTCGTGCGTAA

Upstream 100 bases:

>100_bases
CCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATC
ACCCGTTAAGGAGCAGGCTG

Downstream 100 bases:

>100_bases
ATAAAACCGGGTGATGCAAAAGTAGCCGTTTGATTCACAAGGCCATTGACGCATCGCCCGGTTAGTTTTAACCTTGTCCA
CCGTGATTCACGTTCGTGAA

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILNANAQDVADARANGLSEAMLDRLALTPARLKGIADDV
RQVCNLADPVGQVIDGGVLDSGLRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVVVIQ
DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVITGGIGVCHIYVDESAEIAEALK
VIVNAKTQRPSTCNTVETLLVNKNIAYSFLPALSKQMAESGVTLHADASALAQLQTGPAKVVAVKAEEYDDEFLSLDLNV
KIVSDLDDAIAHIREHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLE
ALTTYKWIGIGDYTIRA

Sequences:

>Translated_417_residues
MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILNANAQDVADARANGLSEAMLDRLALTPARLKGIADDV
RQVCNLADPVGQVIDGGVLDSGLRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVVVIQ
DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVITGGIGVCHIYVDESAEIAEALK
VIVNAKTQRPSTCNTVETLLVNKNIAYSFLPALSKQMAESGVTLHADASALAQLQTGPAKVVAVKAEEYDDEFLSLDLNV
KIVSDLDDAIAHIREHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLE
ALTTYKWIGIGDYTIRA
>Mature_417_residues
MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILNANAQDVADARANGLSEAMLDRLALTPARLKGIADDV
RQVCNLADPVGQVIDGGVLDSGLRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVVVIQ
DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVITGGIGVCHIYVDESAEIAEALK
VIVNAKTQRPSTCNTVETLLVNKNIAYSFLPALSKQMAESGVTLHADASALAQLQTGPAKVVAVKAEEYDDEFLSLDLNV
KIVSDLDDAIAHIREHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLE
ALTTYKWIGIGDYTIRA

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate [H]

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=410, Percent_Identity=36.0975609756098, Blast_Score=264, Evalue=9e-71,
Organism=Homo sapiens, GI62912457, Length=410, Percent_Identity=36.0975609756098, Blast_Score=264, Evalue=1e-70,
Organism=Escherichia coli, GI1786438, Length=417, Percent_Identity=98.8009592326139, Blast_Score=837, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17569969, Length=415, Percent_Identity=35.421686746988, Blast_Score=268, Evalue=5e-72,
Organism=Caenorhabditis elegans, GI17569967, Length=415, Percent_Identity=35.421686746988, Blast_Score=267, Evalue=8e-72,
Organism=Saccharomyces cerevisiae, GI6324899, Length=428, Percent_Identity=38.785046728972, Blast_Score=296, Evalue=4e-81,
Organism=Drosophila melanogaster, GI21357643, Length=410, Percent_Identity=36.0975609756098, Blast_Score=272, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134 [H]

Pfam domain/function: PF00171 Aldedh [H]

EC number: =1.2.1.41 [H]

Molecular weight: Translated: 44725; Mature: 44725

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILNANAQDVADARANGLSE
CCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCHHH
AMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSGLRLERRRVPLGVIGVIYE
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCEEEEEEEEE
ARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVVVIQDALKSCGLPAGAVQAIDNPD
CCCCCCHHHHHHHHHCCCEEEEECCHHHHHCCCEEEEEEHHHHHHCCCCCHHHHHCCCCH
RALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVITGGIGVCHIYVDESAEIAEALK
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEECCCEEEEEEECCCHHHHHHHH
VIVNAKTQRPSTCNTVETLLVNKNIAYSFLPALSKQMAESGVTLHADASALAQLQTGPAK
HHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEECHHHHHHHCCCCEE
VVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIREHGTQHSDAILTRDMRNAQRFVNEVD
EEEEECCCCCCCEEEEEEEEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
SSAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLEALTTYKWIGIGDYTIRA
CCEEEEECCCEECCCCCCCCCCCEEEEHHHHHCCCCCCHHHHHHEEEEECCCEEECC
>Mature Secondary Structure
MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILNANAQDVADARANGLSE
CCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCHHH
AMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSGLRLERRRVPLGVIGVIYE
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCEEEEEEEEE
ARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVVVIQDALKSCGLPAGAVQAIDNPD
CCCCCCHHHHHHHHHCCCEEEEECCHHHHHCCCEEEEEEHHHHHHCCCCCHHHHHCCCCH
RALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVITGGIGVCHIYVDESAEIAEALK
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEECCCEEEEEEECCCHHHHHHHH
VIVNAKTQRPSTCNTVETLLVNKNIAYSFLPALSKQMAESGVTLHADASALAQLQTGPAK
HHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEECHHHHHHHCCCCEE
VVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIREHGTQHSDAILTRDMRNAQRFVNEVD
EEEEECCCCCCCEEEEEEEEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
SSAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLEALTTYKWIGIGDYTIRA
CCEEEEECCCEECCCCCCCCCCCEEEEHHHHHCCCCCCHHHHHHEEEEECCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA