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The map label for this gene is cadB

Identifier: 209397656

GI number: 209397656

Start: 5288347

End: 5289681

Strand: Reverse

Name: cadB

Synonym: ECH74115_5648

Alternate gene names: 209397656

Gene position: 5289681-5288347 (Counterclockwise)

Preceding gene: 209398414

Following gene: 209399154

Centisome position: 94.93

GC content: 49.59

Gene sequence:

>1335_bases
ATGAGTTCTGCCAAGAAGATCGGGCTATTTGCCTGTACCGGTGTTGTTGCCGGTAATATGATGGGGAGCGGTATTGCATT
ATTACCTGCGAACCTAGCAAGTATCGGTGGTATTGCTATCTGGGGTTGGATTATCTCTATTATTGGTGCAATGTCGCTGG
CGTATGTATATGCCCGACTGGCAACAAAAAACCCGCAACAAGGTGGCCCAATTGCTTATGCCGGAGAAATTTCTCCTGCA
TTTGGTTTTCAGACAGGTGTTCTTTATTACCATGCTAACTGGATTGGTAACCTGGCGATTGGTATTACCGCTGTATCTTA
TCTTTCCACCTTCTTCCCAGTATTAAATGATCCTGTTCCGGCGGGTATCGCCTGTATTGCTATCGTCTGGGTATTTACCT
TTGTAAATATGCTCGGCGGTACCTGGGTAAGCCGTTTAACCACTATTGGTCTGGTGCTGGTTCTTATTCCTGTGGTGATG
ACTGCTATTGTTGGCTGGCATTGGTTTGATGCGGCAACTTATGCAGCTAACTGGAATACTGCGGATACCACTGATGGTCA
TGCGATCATTAAAAGTATTCTGCTCTGCCTGTGGGCCTTCGTGGGTGTTGAATCCGCAGCTGTAAGTACTGGTATGGTTA
AAAACCCGAAACGTACCGTTCCGCTGGCAACCATGCTGGGTACTGGTTTAGCAGGTATTGTTTACATCGCTGCGACTCAG
GTGCTTTCCGGTATGTATCCGTCTTCTGTAATGGCGGCTTCCGGTGCTCCGTTTGCAATCAGTGCTTCAACTATCCTCGG
TAACTGGGCTGCGCCGCTGGTTTCTGCATTCACCGCCTTTGCGTGCCTGACTTCTCTGGGCTCCTGGATGATGTTGGTAG
GCCAGGCAGGTGTACGTGCCGCTAACGACGGTAACTTCCCGAAAGTTTATGGTGAAGTCGACAGCAACGGTATTCCGAAA
AAAGGTCTGCTGCTGGCTGCAGTGAAAATGACTGCCCTGATGATCCTCATCACTCTGATGAACTCTGCCGGTGGTAAAGC
ATCTGACCTGTTCGGTGAACTGACCGGTATCGCAGTACTGCTGACTATGCTGCCGTACTTCTACTCTTGCGTTGACCTGA
TTCGTTTTGAAGGCGTTAACATCCGCAACTTTGTCAGCCTGATCTGCTCTGTACTGGGTTGCGTGTTCTGCTTCATCGCG
CTGATGGGCGCAAGCTCCTTCGAGCTGGCAGGTACCTTCATCGTCAGCCTGATTATCCTGATGTTCTACGCTCGCAAAAT
GCACGAGCGCCAGAGCCACTCAATGGATAACCACACCGCGTCTAACGCACATTAA

Upstream 100 bases:

>100_bases
TTTTTTCGTGAGTAATCTTAGCGCCAGTTTGGTCTGGTCAGGAAATAGTTATACATCATGACCCGGACTCCAAATTCAAA
AATGAAATTAGGAGAAGAGC

Downstream 100 bases:

>100_bases
TTAAAAGTATTTTCCGAGGCTCCTCCTTTCATTTTGTCCCATGTGTTGGGAGGGGCCTTTTTTACCTGGAGATATGACTA
TGAACGTTATTGCAATATTG

Product: lysine/cadaverine antiporter

Products: cadaverine [Cytoplasm]; L-lysine [Cytoplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWIISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPA
FGFQTGVLYYHANWIGNLAIGITAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKRTVPLATMLGTGLAGIVYIAATQ
VLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSAFTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPK
KGLLLAAVKMTALMILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVLGCVFCFIA
LMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH

Sequences:

>Translated_444_residues
MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWIISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPA
FGFQTGVLYYHANWIGNLAIGITAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKRTVPLATMLGTGLAGIVYIAATQ
VLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSAFTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPK
KGLLLAAVKMTALMILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVLGCVFCFIA
LMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH
>Mature_443_residues
SSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWIISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAF
GFQTGVLYYHANWIGNLAIGITAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVMT
AIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKRTVPLATMLGTGLAGIVYIAATQV
LSGMYPSSVMAASGAPFAISASTILGNWAAPLVSAFTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKK
GLLLAAVKMTALMILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVLGCVFCFIAL
MGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH

Specific function: Probable cadaverine/lysine antiporter or part of it

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily

Homologues:

Organism=Escherichia coli, GI1790575, Length=444, Percent_Identity=100, Blast_Score=888, Evalue=0.0,
Organism=Escherichia coli, GI2367353, Length=447, Percent_Identity=38.255033557047, Blast_Score=318, Evalue=6e-88,
Organism=Escherichia coli, GI1786908, Length=417, Percent_Identity=32.3741007194245, Blast_Score=198, Evalue=7e-52,
Organism=Escherichia coli, GI1787890, Length=442, Percent_Identity=23.3031674208145, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI87082250, Length=324, Percent_Identity=26.8518518518519, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71982612, Length=338, Percent_Identity=25.1479289940828, Blast_Score=65, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI71982608, Length=338, Percent_Identity=24.2603550295858, Blast_Score=65, Evalue=8e-11,
Organism=Drosophila melanogaster, GI221331183, Length=336, Percent_Identity=25.8928571428571, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI17647653, Length=336, Percent_Identity=25.8928571428571, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24664379, Length=336, Percent_Identity=25.8928571428571, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CADB_ECO57 (P0AAF0)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B91268
- RefSeq:   NP_290766.1
- RefSeq:   NP_313141.1
- ProteinModelPortal:   P0AAF0
- SMR:   P0AAF0
- EnsemblBacteria:   EBESCT00000024488
- EnsemblBacteria:   EBESCT00000057772
- GeneID:   914165
- GeneID:   959991
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5735
- KEGG:   ecs:ECs5114
- GeneTree:   EBGT00050000009006
- HOGENOM:   HBG586921
- OMA:   VGKFIKI
- ProtClustDB:   PRK10435
- BioCyc:   ECOL83334:ECS5114-MONOMER
- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004754
- PANTHER:   PTHR11785
- PIRSF:   PIRSF006060
- TIGRFAMs:   TIGR00905

Pfam domain/function: PF00324 AA_permease

EC number: NA

Molecular weight: Translated: 46666; Mature: 46534

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x13a79720)-; HASH(0x12972c50)-; HASH(0x120a9b48)-; HASH(0x132e7e4c)-; HASH(0x13695e04)-; HASH(0x1325c1bc)-; HASH(0x12e30e40)-; HASH(0x132c752c)-; HASH(0x135ae48c)-; HASH(0x11c44db8)-; HASH(0x139a34b0)-; HASH(0x13a86cec)-; HASH(0x13a02590)-; HASH(0x12345ee0)-;

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHCCCCCCCCCCCH
>Mature Secondary Structure 
SSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWIISIIGAMSLAYVYARL
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGITAVSYLSTFFPVLNDPVP
HCCCCCCCCCEEEECCCCCCCCCHHCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCC
AGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVMTAIVGWHWFDAATYAANWNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKRTVPLATMLGTGLAGIVYIAATQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSAFTAFACLTSLGSWMMLVGQAGVRA
HHHCCCCCHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALMILITLMNSAGGKASDLFGELTGIAVL
CCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LTMLPYFYSCVDLIRFEGVNIRNFVSLICSVLGCVFCFIALMGASSFELAGTFIVSLIIL
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
MFYARKMHERQSHSMDNHTASNAH
HHHHHHHHHHHHCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: cadaverine [Cytoplasm]; Proton [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: cadaverine [Cytoplasm] + Proton [Periplasm] + L-lysine [Periplasm] = cadaverine [Cytoplasm] + L-lysine [Cytoplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796