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The map label for this gene is pinE [H]

Identifier: 209397552

GI number: 209397552

Start: 310772

End: 311326

Strand: Direct

Name: pinE [H]

Synonym: ECH74115_0302

Alternate gene names: 209397552

Gene position: 310772-311326 (Clockwise)

Preceding gene: 209398490

Following gene: 209399437

Centisome position: 5.58

GC content: 49.91

Gene sequence:

>555_bases
ATGCTTATTGGCTATGTACGTGTGTCAACAAATGACCAGAACACAGATCTACAACGTAATGCGCTGAACTGTGCAGGATG
CGAGCTGATTTTTGAAGACAAGATAAGCGGTACAAAGTCCGAAAGGCCGGGACTGAAAAAACTGCTCAGGACATTATCGG
CAGGTGACACTCTGGTTGTCTGGAAACTGGATCGGCTGGGGCGCAGTATGCGGCATCTGGTCATTCTGGTTGAGGAGTTG
CGCGAACGTGGCGTTAATTTTCGCAGCCTGACGGATGCTATTGATACCAGTACCCCAATGGGGCGCTTTTTCTTTCATGT
GATGGGGGCCCTGGCTGAAATGGAACGAGAACTGATTGTTGAACGAACAAAAGCTGGACTGGAAGCTGCTCGCGCACAGG
GACGAATTGGTGGACGTCGTCCCAAACTTACACCAGAACAATGGGCGCAGGCCGGACGATTAATTGCAGCAGGAATTCCT
CGCCAGAAGGTAGCGATCATCTATGATGTTGGTGTATCGACACTGTATAAGAAGTTTCCGGCCGGAGATAAATGA

Upstream 100 bases:

>100_bases
CCCACATCCCGTTTTGCCGCTTCTCCCAAACCAACGTTTATGAAAATGCAGAAATAACGAGCAAATGGCATCATTCCTGC
TTTTGTCAGGGAGATCTACC

Downstream 100 bases:

>100_bases
AACCGTAGCACGTCGTATGCAAGATCGTGCTGCGGTTTATGCTTACCACTTGAAGATTCAAAAATTAGGTGAGTAACGAA
CTGGAGACATAGCTCCTTTT

Product: DNA-invertase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MLIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPGLKKLLRTLSAGDTLVVWKLDRLGRSMRHLVILVEEL
RERGVNFRSLTDAIDTSTPMGRFFFHVMGALAEMERELIVERTKAGLEAARAQGRIGGRRPKLTPEQWAQAGRLIAAGIP
RQKVAIIYDVGVSTLYKKFPAGDK

Sequences:

>Translated_184_residues
MLIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPGLKKLLRTLSAGDTLVVWKLDRLGRSMRHLVILVEEL
RERGVNFRSLTDAIDTSTPMGRFFFHVMGALAEMERELIVERTKAGLEAARAQGRIGGRRPKLTPEQWAQAGRLIAAGIP
RQKVAIIYDVGVSTLYKKFPAGDK
>Mature_184_residues
MLIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPGLKKLLRTLSAGDTLVVWKLDRLGRSMRHLVILVEEL
RERGVNFRSLTDAIDTSTPMGRFFFHVMGALAEMERELIVERTKAGLEAARAQGRIGGRRPKLTPEQWAQAGRLIAAGIP
RQKVAIIYDVGVSTLYKKFPAGDK

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=184, Percent_Identity=96.7391304347826, Blast_Score=370, Evalue=1e-104,
Organism=Escherichia coli, GI1787827, Length=183, Percent_Identity=37.1584699453552, Blast_Score=110, Evalue=4e-26,
Organism=Escherichia coli, GI1787638, Length=183, Percent_Identity=37.1584699453552, Blast_Score=110, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 20511; Mature: 20511

Theoretical pI: Translated: 10.50; Mature: 10.50

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPGLKKLLRTLSAGDTLVV
CEEEEEEEECCCCCCCHHHHCCCHHCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEE
WKLDRLGRSMRHLVILVEELRERGVNFRSLTDAIDTSTPMGRFFFHVMGALAEMERELIV
EEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ERTKAGLEAARAQGRIGGRRPKLTPEQWAQAGRLIAAGIPRQKVAIIYDVGVSTLYKKFP
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCEEECCCCCCCEEEEEECCHHHHHHHCC
AGDK
CCCC
>Mature Secondary Structure
MLIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPGLKKLLRTLSAGDTLVV
CEEEEEEEECCCCCCCHHHHCCCHHCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEE
WKLDRLGRSMRHLVILVEELRERGVNFRSLTDAIDTSTPMGRFFFHVMGALAEMERELIV
EEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ERTKAGLEAARAQGRIGGRRPKLTPEQWAQAGRLIAAGIPRQKVAIIYDVGVSTLYKKFP
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCEEECCCCCCCEEEEEECCHHHHHHHCC
AGDK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]