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The map label for this gene is wrbA [H]

Identifier: 209397525

GI number: 209397525

Start: 1250498

End: 1251094

Strand: Reverse

Name: wrbA [H]

Synonym: ECH74115_1242

Alternate gene names: 209397525

Gene position: 1251094-1250498 (Counterclockwise)

Preceding gene: 209396914

Following gene: 209397797

Centisome position: 22.45

GC content: 53.77

Gene sequence:

>597_bases
ATGGCTAAAGTTCTGGTGCTTTATTATTCCATGTACGGACATATTGAAACGATGGCACGCGCAGTCGCTGAGGGTGCAAG
CAAAGTGGATGGCGCTGAAGTTGTCGTTAAGCGTGTACCGGAAACCATGTCGCCGCAATTATTTGAAAAAGCAGGCGGTA
AAACGCAAACTGCACCGGTTGCAACCCCGCAAGAACTGGCCAATTACGACGCCATTATTTTTGGTACACCTACCCGCTTT
GGCAACATGTCCGGTCAAATGCGTACCTTCCTCGACCAGACGGGCGGCCTGTGGGCTTCCGGCGCACTATACGGAAAACT
GGCGAGCGTCTTTAGTTCCACCGGTACTGGCGGCGGTCAGGAACAAACCATCACATCCACCTGGACGACCCTTGCGCATC
ACGGCATGGTGATTGTCCCCATTGGCTACGCAGCGCAGGAATTATTTGACGTTTCACAGGTTCGCGGCGGTACGCCGTAC
GGCGCAACCACCATCGCAGGCGGTGACGGTTCACGCCAGCCCAGCCAGGAAGAACTGTCTATTGCTCGTTATCAAGGGGA
ATATGTCGCAGGTCTGGCAGTTAAACTTAATGGCTAA

Upstream 100 bases:

>100_bases
TAAGTGGTTGTGAAATTTGCACTCTGAAAAGGACGTCTTATCTTTAAATAAGTGGTAGCGAATCGCTACGGAATAGAGAT
AACACGAGGAGTGGTTAGAA

Downstream 100 bases:

>100_bases
TCTTCAACAGGAGGATACGCATGCCAACTCAAGAAGCGAAAGCTCATCACGTCGGTGAATGGGCATCTCTGCGCAATACG
TCGCCGGAAATAGCCGAGGC

Product: TrpR binding protein WrbA

Products: NA

Alternate protein names: Trp repressor-binding protein [H]

Number of amino acids: Translated: 198; Mature: 197

Protein sequence:

>198_residues
MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMSPQLFEKAGGKTQTAPVATPQELANYDAIIFGTPTRF
GNMSGQMRTFLDQTGGLWASGALYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG

Sequences:

>Translated_198_residues
MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMSPQLFEKAGGKTQTAPVATPQELANYDAIIFGTPTRF
GNMSGQMRTFLDQTGGLWASGALYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG
>Mature_197_residues
AKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMSPQLFEKAGGKTQTAPVATPQELANYDAIIFGTPTRFG
NMSGQMRTFLDQTGGLWASGALYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPYG
ATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG

Specific function: Seems to enhance the formation and/or stability of noncovalent complexes between the trp repressor protein and operator-bearing DNA [H]

COG id: COG0655

COG function: function code R; Multimeric flavodoxin WrbA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Escherichia coli, GI1787239, Length=198, Percent_Identity=98.989898989899, Blast_Score=396, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6320235, Length=190, Percent_Identity=47.3684210526316, Blast_Score=178, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6319849, Length=201, Percent_Identity=45.771144278607, Blast_Score=170, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6319526, Length=203, Percent_Identity=37.4384236453202, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008254
- InterPro:   IPR010089
- InterPro:   IPR005025 [H]

Pfam domain/function: PF03358 FMN_red [H]

EC number: NA

Molecular weight: Translated: 20835; Mature: 20704

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMSPQLFEKAGGKTQTAPV
CCEEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCCC
ATPQELANYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGALYGKLASVFSSTGTGGGQ
CCHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHCCCCEECHHHHHHHHHHHHCCCCCCCC
EQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPYGATTIAGGDGSRQPSQEELS
CCCHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHH
IARYQGEYVAGLAVKLNG
HHHHCCCEEEEEEEEECC
>Mature Secondary Structure 
AKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMSPQLFEKAGGKTQTAPV
CEEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCCC
ATPQELANYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGALYGKLASVFSSTGTGGGQ
CCHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHCCCCEECHHHHHHHHHHHHCCCCCCCC
EQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPYGATTIAGGDGSRQPSQEELS
CCCHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHH
IARYQGEYVAGLAVKLNG
HHHHCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA