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The map label for this gene is lpdA [H]

Identifier: 209397520

GI number: 209397520

Start: 132202

End: 133689

Strand: Direct

Name: lpdA [H]

Synonym: ECH74115_0123

Alternate gene names: 209397520

Gene position: 132202-133689 (Clockwise)

Preceding gene: 209399253

Following gene: 209396098

Centisome position: 2.37

GC content: 52.08

Gene sequence:

>1488_bases
GTGGATAATGGGCGTCATAAAAAAAACGTCAGACCCGCCGGAGATAAATATATAGAGGTCATGATGAGTACTGAAATCAA
AACTCAGGTCGTGGTACTTGGGGCAGGCCCCGCAGGTTACTCCGCTGCCTTCCGTTGCGCTGATTTAGGTCTGGAAACCG
TAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCACTGCTGCACGTA
GCAAAAGTTATCGAAGAAGCCAAAGCGCTGGCTGAACACGGTATCGTCTTCGGCGAACCGAAAACCGATATCGACAAGAT
TCGTACCTGGAAAGAGAAAGTGATCAATCAGCTGACCGGTGGTCTGGCTGGTATGGCGAAAGGCCGCAAAGTCAAAGTGG
TCAACGGTCTGGGTAAATTTACCGGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGTAAAACCGTGATCAACTTCGAC
AACGCGATCATTGCAGCGGGTTCTCGCCCGATTCAACTGCCGTTTATTCCGCATGAAGATCCGCGTATCTGGGACTCTAC
TGACGCGCTGGAACTGAAAGAAGTACCAGAACGCCTGCTGGTAATGGGTGGCGGTATCATCGGTCTGGAAATGGGCACCG
TTTACCACGCGCTGGGTTCACAGATTGACGTGGTTGAAATGTTCGACCAGGTTATCCCGGCAGCTGACAAAGACATCGTT
AAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGA
CGGTATTTATGTGACGATGGAAGGCAAAAAAGCACCAGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTC
GTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCTGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAA
CAGCTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAGCCGATGCTGGCACACAAAGGTGTTCA
CGAAGGTCACGTTGCCGCTGAAGTTATCGCCGGTAAGAAACACTACTTCGATCCGAAAGTTATCCCGTCCATCGCCTATA
CCGAACCAGAAGTTGCATGGGTAGGTCTGACTGAGAAAGAAGCGAAAGAGAAAGGCATCAGCTATGAAACCGCCACCTTC
CCGTGGGCTGCTTCTGGTCGTGCTATCGCTTCCGACTGCGCAGACGGTATGACCAAGCTGATTTTCGACAAAGAATCTCA
CCGTGTGATCGGTGGTGCGATTGTCGGTACTAACGGCGGCGAGCTGCTGGGTGAAATCGGCCTGGCAATTGAAATGGGTT
GTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGCCTGGCGGCAGAAGTGTTC
GAAGGTAGCATTACTGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAA

Upstream 100 bases:

>100_bases
TGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATA
CCGACGGATAGAACGACCCG

Downstream 100 bases:

>100_bases
TTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGCCTGTAATTTACCTTTCC
AGTCGCCTTGCTCCGCGCTC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MDNGRHKKNVRPAGDKYIEVMMSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGVCLNVGCIPSKALLHV
AKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLAGMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFD
NAIIAAGSRPIQLPFIPHEDPRIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDIV
KVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPNGKNLDAGKAGVEVDDRGFIRVDK
QLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEVIAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATF
PWAASGRAIASDCADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVF
EGSITDLPNPKAKKK

Sequences:

>Translated_495_residues
MDNGRHKKNVRPAGDKYIEVMMSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGVCLNVGCIPSKALLHV
AKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLAGMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFD
NAIIAAGSRPIQLPFIPHEDPRIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDIV
KVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPNGKNLDAGKAGVEVDDRGFIRVDK
QLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEVIAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATF
PWAASGRAIASDCADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVF
EGSITDLPNPKAKKK
>Mature_495_residues
MDNGRHKKNVRPAGDKYIEVMMSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGVCLNVGCIPSKALLHV
AKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLAGMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFD
NAIIAAGSRPIQLPFIPHEDPRIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDIV
KVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPNGKNLDAGKAGVEVDDRGFIRVDK
QLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEVIAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATF
PWAASGRAIASDCADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVF
EGSITDLPNPKAKKK

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=454, Percent_Identity=43.6123348017621, Blast_Score=345, Evalue=6e-95,
Organism=Homo sapiens, GI50301238, Length=457, Percent_Identity=28.4463894967177, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI22035672, Length=429, Percent_Identity=29.3706293706294, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI148277065, Length=437, Percent_Identity=27.6887871853547, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI148277071, Length=437, Percent_Identity=27.6887871853547, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI33519430, Length=437, Percent_Identity=27.6887871853547, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI33519428, Length=437, Percent_Identity=27.6887871853547, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI33519426, Length=437, Percent_Identity=27.6887871853547, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI291045266, Length=441, Percent_Identity=26.7573696145125, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI291045268, Length=432, Percent_Identity=25.2314814814815, Blast_Score=93, Evalue=5e-19,
Organism=Escherichia coli, GI1786307, Length=474, Percent_Identity=100, Blast_Score=956, Evalue=0.0,
Organism=Escherichia coli, GI87082354, Length=458, Percent_Identity=27.7292576419214, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI87081717, Length=455, Percent_Identity=28.3516483516484, Blast_Score=178, Evalue=7e-46,
Organism=Escherichia coli, GI1789915, Length=437, Percent_Identity=27.9176201372998, Blast_Score=147, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI32565766, Length=468, Percent_Identity=38.6752136752137, Blast_Score=322, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI17557007, Length=476, Percent_Identity=27.7310924369748, Blast_Score=132, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI71983429, Length=436, Percent_Identity=27.0642201834862, Blast_Score=132, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI71983419, Length=436, Percent_Identity=27.0642201834862, Blast_Score=131, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI71982272, Length=442, Percent_Identity=26.6968325791855, Blast_Score=115, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6321091, Length=456, Percent_Identity=40.7894736842105, Blast_Score=307, Evalue=2e-84,
Organism=Saccharomyces cerevisiae, GI6325240, Length=484, Percent_Identity=26.6528925619835, Blast_Score=175, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6325166, Length=474, Percent_Identity=27.2151898734177, Blast_Score=150, Evalue=4e-37,
Organism=Drosophila melanogaster, GI21358499, Length=457, Percent_Identity=39.6061269146608, Blast_Score=322, Evalue=4e-88,
Organism=Drosophila melanogaster, GI24640549, Length=462, Percent_Identity=29.004329004329, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24640553, Length=462, Percent_Identity=29.004329004329, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24640551, Length=462, Percent_Identity=29.004329004329, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI17737741, Length=479, Percent_Identity=26.3048016701461, Blast_Score=115, Evalue=8e-26,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 53130; Mature: 53130

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNGRHKKNVRPAGDKYIEVMMSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYN
CCCCCCCCCCCCCCCCEEEEEEECCCEEEEEEEECCCCCCHHHHHHHHCCHHEEEHHHHH
TLGGVCLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTG
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHC
GLAGMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHED
CHHHHCCCCEEEEEECCHHCCCCCEEEEECCCCCEEEEECCEEEECCCCEEEECCCCCCC
PRIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDIV
CCCCCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHH
KVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPNGKN
HHHHHHHHHHHCEEEEEEEEEEEECCCCEEEEECCCCCCCCCHHHCEEEEEEECCCCCCC
LDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEVIAGKK
CCCCCCCCEECCCCEEEECHHHHCCCCEEEEEHHHHCCCHHHHCCCCCCCHHHHHHHCCC
HYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDCADGMTKL
CCCCCCCCCCEECCCCCEEEEECCHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHH
IFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVF
HCCCCCCEEEEEEEEECCCHHHHHHCCEEEEECCCCCCEEEEEECCCCHHHHHCCHHHHH
EGSITDLPNPKAKKK
CCCCCCCCCCCCCCC
>Mature Secondary Structure
MDNGRHKKNVRPAGDKYIEVMMSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYN
CCCCCCCCCCCCCCCCEEEEEEECCCEEEEEEEECCCCCCHHHHHHHHCCHHEEEHHHHH
TLGGVCLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTG
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHC
GLAGMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHED
CHHHHCCCCEEEEEECCHHCCCCCEEEEECCCCCEEEEECCEEEECCCCEEEECCCCCCC
PRIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDIV
CCCCCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHH
KVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPNGKN
HHHHHHHHHHHCEEEEEEEEEEEECCCCEEEEECCCCCCCCCHHHCEEEEEEECCCCCCC
LDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEVIAGKK
CCCCCCCCEECCCCEEEECHHHHCCCCEEEEEHHHHCCCHHHHCCCCCCCHHHHHHHCCC
HYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDCADGMTKL
CCCCCCCCCCEECCCCCEEEEECCHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHH
IFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVF
HCCCCCCEEEEEEEEECCCHHHHHHCCEEEEECCCCCCEEEEEECCCCHHHHHCCHHHHH
EGSITDLPNPKAKKK
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]