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The map label for this gene is insI1 [H]

Identifier: 209397386

GI number: 209397386

Start: 2518271

End: 2519335

Strand: Reverse

Name: insI1 [H]

Synonym: ECH74115_2677

Alternate gene names: 209397386

Gene position: 2519335-2518271 (Counterclockwise)

Preceding gene: 209400207

Following gene: 209399284

Centisome position: 45.21

GC content: 46.2

Gene sequence:

>1065_bases
TTGACGGACCTCGGGGGAAAAACGTGTATTTTTAGTCATCCTGTTTACCTCTTTCTCAGGGAGTTTAGTCTCCAGGATTC
CCGGGGCGGTTCACTGTCTGAGCGCGAGGAGATACGAGCTGGTTTGTCAGCCAAAATGAGCATTCGTGCGATAGCTACTG
CGCTGAATCGCAGTCCTTCGACGATCTCACGTGAAGTTCAGCGTAATCGGGGCAGACGCTATTACAAAGCTGTTGATGCT
AATAACCGAGCCAACAGAATGGCGAAAAGGCCAAAACCGTGCTTACTGGATCAAAATTTACCATTGCGAAAGCTTGTTCT
GGAAAAGCTGGAGATGAAATGGTCTCCAGAGCAAATATCAGGATGGTTAAGGCGAACAAAACCACGTCAAAAAACGCTGC
GAATATCACCTGAGACAATTTATAAAACGCTGTACTTTCGTAGCCGTGAAGCGCTACACCACCTGAATATACAGCATCTG
CGACGGTCGCATAGCCTTCGCCATGGCAGGCGTCATACCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAAC
ACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTAGGGCATTGGGAGGGCGATTTAGTCTCAGGTACAA
AAAACTCTCATATAGCCACACTTGTAGACCGAAAATCACGTTATACGATCATCCTCAGACTCAGGGGCAAAGATTCTGTC
TCAGTAAATCAGGCTCTTACCGACAAATTCCTGAGTTTACCGTCAGAACTCAGAAAATCACTGACATGGGACAGAGGAAT
GGAACTGGCCAGACATCTAGAATTTACTGTCAGCACCGGCGTTAAAGTTTACTTCTGCGATCCTCAGAGTCCTTGGCAGC
GGGGAACAAATGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACAT
GAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGA
AAGGGGTGTTGCATTGACAGATTGA

Upstream 100 bases:

>100_bases
GAGTCTCTGGTGTACAGCCAATCTTTGGGGCAATGGAACAAATTGCCGCCCATTGTGAGTCATATTCGCCCTGACTTTCC
AGAACCATACGAACTGCTCG

Downstream 100 bases:

>100_bases
ATCTACAAGTGCCTTTTTCTGTCAGCGGTTCTCCTTTCCAGCCAATGAGAACCGTCAGGCGCGCGCCCCGTGGCGGTAGC
TGCAACTGACCATCTGCATC

Product: transposase InsI for insertion sequence element IS30B/C/D

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MTDLGGKTCIFSHPVYLFLREFSLQDSRGGSLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRRYYKAVDA
NNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQKTLRISPETIYKTLYFRSREALHHLNIQHL
RRSHSLRHGRRHTRKGERGTINIVNGTPIHERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDSV
SVNQALTDKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIRQYFPKKTCLAQYTQH
ELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD

Sequences:

>Translated_354_residues
MTDLGGKTCIFSHPVYLFLREFSLQDSRGGSLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRRYYKAVDA
NNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQKTLRISPETIYKTLYFRSREALHHLNIQHL
RRSHSLRHGRRHTRKGERGTINIVNGTPIHERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDSV
SVNQALTDKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIRQYFPKKTCLAQYTQH
ELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD
>Mature_353_residues
TDLGGKTCIFSHPVYLFLREFSLQDSRGGSLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRRYYKAVDAN
NRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQKTLRISPETIYKTLYFRSREALHHLNIQHLR
RSHSLRHGRRHTRKGERGTINIVNGTPIHERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDSVS
VNQALTDKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIRQYFPKKTCLAQYTQHE
LDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG2826

COG function: function code L; Transposase and inactivated derivatives, IS30 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1790736, Length=324, Percent_Identity=99.6913580246914, Blast_Score=664, Evalue=0.0,
Organism=Escherichia coli, GI1787671, Length=324, Percent_Identity=99.6913580246914, Blast_Score=664, Evalue=0.0,
Organism=Escherichia coli, GI1786450, Length=324, Percent_Identity=99.3827160493827, Blast_Score=663, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR001598 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 41040; Mature: 40909

Theoretical pI: Translated: 11.34; Mature: 11.34

Prosite motif: PS50994 INTEGRASE ; PS01043 TRANSPOSASE_IS30

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLGGKTCIFSHPVYLFLREFSLQDSRGGSLSEREEIRAGLSAKMSIRAIATALNRSPS
CCCCCCCEEEECCHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCHH
TISREVQRNRGRRYYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQIS
HHHHHHHHHCCCEEEEEECCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHH
GWLRRTKPRQKTLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGT
HHHHHCCCHHHEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCE
INIVNGTPIHERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDSV
EEEECCCCCHHHHCCCCHHHCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCC
SVNQALTDKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENT
HHHHHHHHHHHHCHHHHHHHCCHHHHHHHHHHHHHEEECCEEEEEECCCCHHHCCCCCCC
NGLIRQYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHCCCCCCC
>Mature Secondary Structure 
TDLGGKTCIFSHPVYLFLREFSLQDSRGGSLSEREEIRAGLSAKMSIRAIATALNRSPS
CCCCCCEEEECCHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCHH
TISREVQRNRGRRYYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQIS
HHHHHHHHHCCCEEEEEECCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHH
GWLRRTKPRQKTLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGT
HHHHHCCCHHHEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCE
INIVNGTPIHERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDSV
EEEECCCCCHHHHCCCCHHHCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCC
SVNQALTDKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENT
HHHHHHHHHHHHCHHHHHHHCCHHHHHHHHHHHHHEEECCEEEEEECCCCHHHCCCCCCC
NGLIRQYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]