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The map label for this gene is hyfA [H]

Identifier: 209397372

GI number: 209397372

Start: 3421413

End: 3422030

Strand: Direct

Name: hyfA [H]

Synonym: ECH74115_3703

Alternate gene names: 209397372

Gene position: 3421413-3422030 (Clockwise)

Preceding gene: 209396765

Following gene: 209398249

Centisome position: 61.4

GC content: 56.63

Gene sequence:

>618_bases
ATGAACCGCTTTGTGGTGGCCGAACCACTGTGGTGTACAGGATGTAATACCTGTCTCGCTGCCTGTTCGGACGTGCATAA
AACGCAAGGTTTACAGCAACACCCGCGCCTGGCCCTGGCGAAGACGTCAACACTCACTGCCCCTGTCGTGTGTCATCACT
GTGAGGAAGCCCCTTGCGTGCAGGTCTGCCCGGTCAATGCCATCTCCCAGAGGGATGATGCGATCCAACTCAACGAAAGC
CTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATGC
CCCTGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGC
GCTGGGAACCGGGTGTCCAGACCGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCTGCCTGCGTTCGC
GCTTGCCCGAATCAGGCGTTACGGCTGATCACCGACGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGC
AAGCTGGTTTGCCAATGGCAGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAA

Upstream 100 bases:

>100_bases
TCAGTTAATTCATAACTTATTGAATATATTAAATTAATCATACTGGCATCCTTTATGCATTATGAAATGCAATGTTTCAT
ATCATTTTCAAGGAGCCGAC

Downstream 100 bases:

>100_bases
TGGATGCCCTGCAATTATTAACCTGGTCGCTGATCCTCTATCTGTTTGCCAGTCTGGCTTCGCTGTTTTTACTCGGTCTG
GACAGACTGGCTATTAAGCT

Product: hydrogenase-4 component A

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTSTLTAPVVCHHCEEAPCVQVCPVNAISQRDDAIQLNES
LCIGCKLCAVVCPFGAISASGSRPVNAPAQYVFQAEGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
ACPNQALRLITDDSLQRQMKEKQRLAASWFANGREDPLSLTQEQR

Sequences:

>Translated_205_residues
MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTSTLTAPVVCHHCEEAPCVQVCPVNAISQRDDAIQLNES
LCIGCKLCAVVCPFGAISASGSRPVNAPAQYVFQAEGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
ACPNQALRLITDDSLQRQMKEKQRLAASWFANGREDPLSLTQEQR
>Mature_205_residues
MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTSTLTAPVVCHHCEEAPCVQVCPVNAISQRDDAIQLNES
LCIGCKLCAVVCPFGAISASGSRPVNAPAQYVFQAEGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
ACPNQALRLITDDSLQRQMKEKQRLAASWFANGREDPLSLTQEQR

Specific function: Probable electron transfer protein for hydrogenase 4 [H]

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI87082114, Length=205, Percent_Identity=97.5609756097561, Blast_Score=409, Evalue=1e-116,
Organism=Escherichia coli, GI1789079, Length=185, Percent_Identity=50.8108108108108, Blast_Score=194, Evalue=3e-51,
Organism=Escherichia coli, GI87082180, Length=203, Percent_Identity=34.4827586206897, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1788811, Length=188, Percent_Identity=38.2978723404255, Blast_Score=118, Evalue=2e-28,
Organism=Escherichia coli, GI87082179, Length=202, Percent_Identity=36.1386138613861, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1789067, Length=199, Percent_Identity=34.1708542713568, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI2367245, Length=190, Percent_Identity=34.2105263157895, Blast_Score=103, Evalue=6e-24,
Organism=Escherichia coli, GI2367345, Length=199, Percent_Identity=27.1356783919598, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI226510944, Length=182, Percent_Identity=30.2197802197802, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 22258; Mature: 22258

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
8.8 %Cys+Met (Translated Protein)
7.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
8.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTSTLTAPVVCHHCEEAPCV
CCCEEECCCCHHCCHHHHHHHHHHHHHHCCHHHCCCEEEECCCCCCHHHHHHCCCCCCCE
QVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGSRPVNAPAQYVFQAEGSLK
EECCCCHHHCCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHEEEECCCCCC
DGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPEGPACVRACPNQALRLITDDSLQRQMK
CCCCCCCCHHEEEEECCCCEEEEEEECHHHCCCCCCHHHHHCCHHHEEEECCHHHHHHHH
EKQRLAASWFANGREDPLSLTQEQR
HHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTSTLTAPVVCHHCEEAPCV
CCCEEECCCCHHCCHHHHHHHHHHHHHHCCHHHCCCEEEECCCCCCHHHHHHCCCCCCCE
QVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGSRPVNAPAQYVFQAEGSLK
EECCCCHHHCCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHEEEECCCCCC
DGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPEGPACVRACPNQALRLITDDSLQRQMK
CCCCCCCCHHEEEEECCCCEEEEEEECHHHCCCCCCHHHHHCCHHHEEEECCHHHHHHHH
EKQRLAASWFANGREDPLSLTQEQR
HHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2016588; 9205837; 9278503 [H]