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The map label for this gene is sucB

Identifier: 209397255

GI number: 209397255

Start: 841438

End: 842655

Strand: Direct

Name: sucB

Synonym: ECH74115_0819

Alternate gene names: 209397255

Gene position: 841438-842655 (Clockwise)

Preceding gene: 209399395

Following gene: 209397431

Centisome position: 15.1

GC content: 55.67

Gene sequence:

>1218_bases
ATGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATGCCACCGTCGCAACCTGGCATAAAAAACC
CGGTGACGCAGTCGTACGTGATGAAGTGCTGGTAGAAATCGAAACTGACAAAGTGGTACTGGAAGTACCGGCATCAGCAG
ACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGGTAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGC
AACAGCGCCGGTAAAGAAACCAGCGCCAAATCTGAAGAGAAAGCGTCCACTCCGGCGCAACGCCAGCAGGCGTCTCTGGA
AGAGCAAAACAACGATGCGTTAAGCCCGGCGATCCGTCGCCTGCTGGCTGAACATAACCTCGACGCCAGCGCCATTAAAG
GCACCGGTGTGGGTGGTCGTCTGACCCGTGAAGATGTGGAAAAACATCTGGCGAAAGCTCCGGCGAAAGAGTCTGCACCG
GCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGCGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCG
TGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTA
TGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAA
GCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAATGCTTCTATCGACGGCGATGACGTGGTTTACCACAACTATTT
CGACGTCAGCATGGCGGTTTCTACGCCGCGCGGCCTGGTGACGCCGGTACTGCGTGATGTCGATACCCTCGGCATGGCAG
ACATCGAGAAGAAAATCAAAGAGCTGGCAGTTAAAGGCCGTGACGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAAC
TTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGCCGATCATCAACCCGCCGCAGAGCGCAATTCTGGG
TATGCACGCTATCAAAGATCGTCCGATGGCTGTGAATGGTCAGGTTGAGATCCTGCCGATGATGTACCTGGCGCTGTCCT
ACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGT
CTGCTGCTGGACGTGTAG

Upstream 100 bases:

>100_bases
CCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCAGAAACAGCAACAAGATCTGGTTAATGACGCGCTGAACGTC
GAATAAATAAAGGATACACA

Downstream 100 bases:

>100_bases
TAGTTTAAGTTTCACCTGCACTGTAGACCGACGTGGATAGGGTGTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGG
ATGGACAGAACACATGAACT

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDKVVLEVPASADGILDAVLEDEGTTVTSRQILGRLREG
NSAGKETSAKSEEKASTPAQRQQASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGEAFEKRHGIRLGFMSFYVK
AVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTPRGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGN
FTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPTR
LLLDV

Sequences:

>Translated_405_residues
MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDKVVLEVPASADGILDAVLEDEGTTVTSRQILGRLREG
NSAGKETSAKSEEKASTPAQRQQASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGEAFEKRHGIRLGFMSFYVK
AVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTPRGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGN
FTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPTR
LLLDV
>Mature_404_residues
SSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDKVVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGN
SAGKETSAKSEEKASTPAQRQQASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAPA
AAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGEAFEKRHGIRLGFMSFYVKA
VVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTPRGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNF
TITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPTRL
LLDV

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI19923748, Length=250, Percent_Identity=58, Blast_Score=300, Evalue=2e-81,
Organism=Homo sapiens, GI203098753, Length=453, Percent_Identity=28.476821192053, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI203098816, Length=453, Percent_Identity=28.476821192053, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI31711992, Length=298, Percent_Identity=32.8859060402685, Blast_Score=158, Evalue=7e-39,
Organism=Homo sapiens, GI110671329, Length=433, Percent_Identity=27.0207852193995, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI260898739, Length=168, Percent_Identity=34.5238095238095, Blast_Score=105, Evalue=6e-23,
Organism=Escherichia coli, GI1786946, Length=405, Percent_Identity=100, Blast_Score=819, Evalue=0.0,
Organism=Escherichia coli, GI1786305, Length=406, Percent_Identity=30.7881773399015, Blast_Score=166, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI25146366, Length=399, Percent_Identity=42.6065162907268, Blast_Score=320, Evalue=6e-88,
Organism=Caenorhabditis elegans, GI17560088, Length=444, Percent_Identity=31.5315315315315, Blast_Score=174, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=420, Percent_Identity=25.4761904761905, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17538894, Length=312, Percent_Identity=31.4102564102564, Blast_Score=143, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6320352, Length=405, Percent_Identity=43.4567901234568, Blast_Score=323, Evalue=3e-89,
Organism=Saccharomyces cerevisiae, GI6324258, Length=449, Percent_Identity=27.3942093541203, Blast_Score=152, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24645909, Length=235, Percent_Identity=59.1489361702128, Blast_Score=291, Evalue=4e-79,
Organism=Drosophila melanogaster, GI18859875, Length=428, Percent_Identity=28.5046728971963, Blast_Score=161, Evalue=8e-40,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ODO2_ECO57 (P0AFG7)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   G85573
- PIR:   H90722
- RefSeq:   NP_286443.1
- RefSeq:   NP_308779.1
- PDB:   2CYU
- PDBsum:   2CYU
- ProteinModelPortal:   P0AFG7
- MINT:   MINT-1242594
- PRIDE:   P0AFG7
- EnsemblBacteria:   EBESCT00000027100
- EnsemblBacteria:   EBESCT00000059698
- GeneID:   917484
- GeneID:   957834
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0881
- KEGG:   ecs:ECs0752
- GeneTree:   EBGT00050000011101
- HOGENOM:   HBG630916
- OMA:   MLTTYNE
- ProtClustDB:   PRK05704
- BioCyc:   ECOL83334:ECS0752-MONOMER
- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255
- Gene3D:   G3DSA:4.10.320.10
- Gene3D:   G3DSA:3.30.559.10
- TIGRFAMs:   TIGR01347

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding; SSF47005 E3_bd; SSF51230 Hybrid_motif

EC number: =2.3.1.61

Molecular weight: Translated: 44012; Mature: 43881

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS50968 BIOTINYL_LIPOYL; PS00189 LIPOYL

Important sites: ACT_SITE 376-376 ACT_SITE 380-380

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDKVVLEVPASADGILDAV
CCCCCEECCCCCHHHHHHHHHHHCCCCCCCEEECEEEEEEECCEEEEECCCCCCHHHHHH
LEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQQASLEEQNNDALSPAIRR
HCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHCCCCHHHHHHHH
LLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAPAAAAPAAQPALAARSEKRVP
HHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCC
MTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGEAFEKRHGIRLGFMSFYVK
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHH
AVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTPRGLVTPVLRDVDTLGMADIEKKIK
HHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHH
ELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVNG
HHHHCCCCCCEEEEECCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECC
QVEILPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPTRLLLDV
CEEHHHHHHHHHHCCCCCCCCCCCCCHHEEHHHHHCCHHHHHHCC
>Mature Secondary Structure 
SSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDKVVLEVPASADGILDAV
CCCCEECCCCCHHHHHHHHHHHCCCCCCCEEECEEEEEEECCEEEEECCCCCCHHHHHH
LEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQQASLEEQNNDALSPAIRR
HCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHCCCCHHHHHHHH
LLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAPAAAAPAAQPALAARSEKRVP
HHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCC
MTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGEAFEKRHGIRLGFMSFYVK
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHH
AVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTPRGLVTPVLRDVDTLGMADIEKKIK
HHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHH
ELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVNG
HHHHCCCCCCEEEEECCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECC
QVEILPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPTRLLLDV
CEEHHHHHHHHHHCCCCCCCCCCCCCHHEEHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796