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The map label for this gene is gatC [H]

Identifier: 209397224

GI number: 209397224

Start: 4458469

End: 4459827

Strand: Direct

Name: gatC [H]

Synonym: ECH74115_4825

Alternate gene names: 209397224

Gene position: 4458469-4459827 (Clockwise)

Preceding gene: 209399527

Following gene: 209398014

Centisome position: 80.01

GC content: 50.18

Gene sequence:

>1359_bases
ATGAACGATATCGCGCATACCCTCTATAATATTGTGCAATATATATTGGGATTTGGCCCGACGGTAATGTTGCCGTTGGT
GTTATTTATTCTTGCTCTCTGTTTTAAAGTAAAACCCGCTAAAGCCTTACGTTCGTCATTAACAGTCGGCATTGGTTTTG
TCGGTATTTATGCCATTTTCGATATTCTGACCAGCAATGTCGGGCCAGCGGCCCAGGCGATGGTTGAACGCACCGGAATT
AATTTACCGGTGGTAGATTTAGGCTGGCCGCCGCTTTCCGCTATTACATGGGGTTCGCCAATTGCCCCGTTTGTTATTCC
CCTGACCATTCTGATTAACGTGGCGATGCTGGCGTTAAATAAAACCCGTACCGTTGACGTAGATATGTGGAACTACTGGC
ATTTTGCCCTTGCTGGTACGTTGGTTTATTACAGCACCGGCAGCCTGTTCTTTGGTTTGCTGGCGGCGGCGATTGCTGCG
GTGGTAGTACTTAAACTCGCCGACTGGTCTGCGCCACTGGTACAAAAATACTTTGGACTGGAAGGGATCTCATTGCCGAC
GCTCTCTTCGGTGGTGTTCTTCCCGGTCGGTCTGCTGGTCGACAAAATCATCGACCATATCCCTGGCCTCAATCGTATTC
ATATCGACCCGGAAACCGTACAGAAAAAGTTTGGCATCTTCGGCGAACCGATGATGGTTGGCACTATTCTGGGCATTCTG
CTCGGCGTAATTGCCGGATACGATTTCAAAAAAGTATTGCTGCTTGGCATCAGCATTGGCGGTGTGATGTTCATCCTGCC
ACGCATGGTACGCATCCTGATGGAAGGTTTATTACCGCTGTCTGAAGCTATTAAAAAGTATCTCAATGCCAAATACCCTG
ACCGTGACGATCTCTATATCGGCCTGGATATCGCCGTTGCCGTAGGTAATCCGGCGATTATCTCCACCGCCCTGCTGCTA
ACGCCAATCTCGGTCTTTATCGCGTTTGTCCTTCCGGGTAATGAAGTCCTGCCGCTTGGCGACCTTGCCAACCTGGCGGT
AATGGCGTCGATGATTGCTTTAGCCAGCCGTGGCAATATTTTCCGCACCGTTCTGGCGGCGATCCCGGTGATTATTGCCG
ACCTATGGATTGCTACCAAAATCGCGCCGTTTATTACCGGAATGGCGAAAGACGTTAACTTCAAATTTGCCGAAGGCTCC
AGCGGCCAGGTTTCCAGTTTTCTTGATGGCGGTAACCCGTTCCGCTTCTGGCTGCTGGAAATCTTCAACGGCAATCTCAT
CGCCATTGGTCTGGTGCCGGTTATCGCCCTGGTACTGTATGGCATTTTCCGAATGACGCGGAGCACGGTTTATGCCTGA

Upstream 100 bases:

>100_bases
ATTGTTTCTATCCTTAAAAAGTAATTAATAATAATTAATTTCAACCCACTCCATCAGGCGCTAACGCGAGGTGCGCTCTC
GCCTGAAAAAGAGGTTTATT

Downstream 100 bases:

>100_bases
TAACAGCGCAGCTATCGTTATCGATATTGGCACCACCAATTGCAAAGTCACCTGCTTTTCCTGCCTGGACGCAACGACGT
TGGGCGCGCATAAATTCGTG

Product: PEP-dependent sugar transporting PTS family, IIC component

Products: galactitol-1-phosphate [Cytoplasm]; pyruvate [C]

Alternate protein names: EIIC-Gat; PTS system galactitol-specific EIIC component [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALCFKVKPAKALRSSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGI
NLPVVDLGWPPLSAITWGSPIAPFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPETVQKKFGIFGEPMMVGTILGIL
LGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEGLLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLL
TPISVFIAFVLPGNEVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDVNFKFAEGS
SGQVSSFLDGGNPFRFWLLEIFNGNLIAIGLVPVIALVLYGIFRMTRSTVYA

Sequences:

>Translated_452_residues
MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALCFKVKPAKALRSSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGI
NLPVVDLGWPPLSAITWGSPIAPFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPETVQKKFGIFGEPMMVGTILGIL
LGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEGLLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLL
TPISVFIAFVLPGNEVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDVNFKFAEGS
SGQVSSFLDGGNPFRFWLLEIFNGNLIAIGLVPVIALVLYGIFRMTRSTVYA
>Mature_452_residues
MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALCFKVKPAKALRSSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGI
NLPVVDLGWPPLSAITWGSPIAPFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPETVQKKFGIFGEPMMVGTILGIL
LGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEGLLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLL
TPISVFIAFVLPGNEVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDVNFKFAEGS
SGQVSSFLDGGNPFRFWLLEIFNGNLIAIGLVPVIALVLYGIFRMTRSTVYA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3775

COG function: function code G; Phosphotransferase system, galactitol-specific IIC component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788408, Length=439, Percent_Identity=40.5466970387244, Blast_Score=342, Evalue=3e-95,
Organism=Escherichia coli, GI1790758, Length=411, Percent_Identity=39.9026763990268, Blast_Score=298, Evalue=4e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013853
- InterPro:   IPR013014
- InterPro:   IPR004703 [H]

Pfam domain/function: PF03611 EIIC-GAT [H]

EC number: NA

Molecular weight: Translated: 48723; Mature: 48723

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALCFKVKPAKALRSSLTVGIGFVGIYAIF
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
DILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIAPFVIPLTILINVAMLALN
HHHHCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
KTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAAVVVLKLADWSAPLVQKYFGL
CCCEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCC
EGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPETVQKKFGIFGEPMMVGTILGIL
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHCCCCCHHHHHHHHHHH
LGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEGLLPLSEAIKKYLNAKYPDRDDLYI
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEE
GLDIAVAVGNPAIISTALLLTPISVFIAFVLPGNEVLPLGDLANLAVMASMIALASRGNI
EEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHCCHH
FRTVLAAIPVIIADLWIATKIAPFITGMAKDVNFKFAEGSSGQVSSFLDGGNPFRFWLLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHCCCCHHHHHHHH
IFNGNLIAIGLVPVIALVLYGIFRMTRSTVYA
HHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALCFKVKPAKALRSSLTVGIGFVGIYAIF
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
DILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIAPFVIPLTILINVAMLALN
HHHHCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
KTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAAVVVLKLADWSAPLVQKYFGL
CCCEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCC
EGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPETVQKKFGIFGEPMMVGTILGIL
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHCCCCCHHHHHHHHHHH
LGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEGLLPLSEAIKKYLNAKYPDRDDLYI
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEE
GLDIAVAVGNPAIISTALLLTPISVFIAFVLPGNEVLPLGDLANLAVMASMIALASRGNI
EEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHCCHH
FRTVLAAIPVIIADLWIATKIAPFITGMAKDVNFKFAEGSSGQVSSFLDGGNPFRFWLLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHCCCCHHHHHHHH
IFNGNLIAIGLVPVIALVLYGIFRMTRSTVYA
HHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoenolpyruvate; galactitol [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + galactitol [Periplasm] = galactitol-1-phosphate [Cytoplasm] + pyruvate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]