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The map label for this gene is rcnA [H]

Identifier: 209397100

GI number: 209397100

Start: 2858425

End: 2859282

Strand: Direct

Name: rcnA [H]

Synonym: ECH74115_3089

Alternate gene names: 209397100

Gene position: 2858425-2859282 (Clockwise)

Preceding gene: 209398006

Following gene: 209396065

Centisome position: 51.3

GC content: 50.7

Gene sequence:

>858_bases
ATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCA
TGGCCTGGAACCAGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAGGCACCATTAAACAGGCGGTGA
TGCTCGGACTGGCAGCAACCATTTCGCATACCGCAGTGGTCTGGTTGATTGCCTTTGGCGGGATGGTGATCAGCAAGCGC
TTTACTGCTCAATCAGCAGAACCGTGGCTCCAGTTGATTTCCGCAGTGATCATTATTAGCACCGCTTTCTGGATGTTCTG
GCGTACCTGGCGCGGCGAACGCAACTGGCTGGAGAATATGCATGAGCATCATCACCACGATCACGAACATCATCAAGACC
ACGACCACGACCACGACCACGACCACGACCACGACCACGAACATCATCACCATCATGAACATGGCGACAACGAAGAGTAT
CAGGATGCCCATGCACGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTT
GTTATTTGGCTTAACAGGTGGCCTTATTCCCTGCCCGGCAGCAATTACCGTGCTGTTGATTTGCATTCAGTTGAAAGCCC
TGACACTGGGCGCAACACTGGTCGTCAGTTTCAGCATCGGTCTGGCACTAACGCTGGTTACAGTAGGCGTTGGAGCGGCA
ATCAGTGTTCAACAAGTTGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCTATTTTTCCAGTCTGTT
AATTGGTTTAGTCGGGGTGTATATGGGAGTGCATGGCTTTATGGGTATTATGAGATAA

Upstream 100 bases:

>100_bases
GAACATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGAGTAGAATCAGATTGCCGAAT
TAATACTAAGAATTATTATC

Downstream 100 bases:

>100_bases
AACAATCACCTGGGCTTCGCTATCCTTTTAGCCCAGGATGATTCTTAAATCAGCTTATTCCGCTCACTTCTCGCTCAATG
AGGATGCTTTTGCTACATCC

Product: nickel/cobalt efflux protein RcnA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIAIKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKR
FTAQSAEPWLQLISAVIIISTAFWMFWRTWRGERNWLENMHEHHHHDHEHHQDHDHDHDHDHDHDHEHHHHHEHGDNEEY
QDAHARAHANDIKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLALTLVTVGVGAA
ISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR

Sequences:

>Translated_285_residues
MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIAIKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKR
FTAQSAEPWLQLISAVIIISTAFWMFWRTWRGERNWLENMHEHHHHDHEHHQDHDHDHDHDHDHDHEHHHHHEHGDNEEY
QDAHARAHANDIKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLALTLVTVGVGAA
ISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR
>Mature_284_residues
TEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIAIKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRF
TAQSAEPWLQLISAVIIISTAFWMFWRTWRGERNWLENMHEHHHHDHEHHQDHDHDHDHDHDHDHEHHHHHEHGDNEEYQ
DAHARAHANDIKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLALTLVTVGVGAAI
SVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR

Specific function: Efflux system for nickel and cobalt [H]

COG id: COG2215

COG function: function code R; ABC-type uncharacterized transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NiCoT transporter (TC 2.A.52) family. RcnA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788423, Length=285, Percent_Identity=90.1754385964912, Blast_Score=477, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011541 [H]

Pfam domain/function: PF03824 NicO [H]

EC number: NA

Molecular weight: Translated: 31882; Mature: 31751

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIAIKGTIKQAVMLGLAAT
CCHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTAFWMFWRTWRGERNWLENM
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
HEHHHHDHEHHQDHDHDHDHDHDHDHEHHHHHEHGDNEEYQDAHARAHANDIKRRFDGRE
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
VTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLALTLVTVGVGAA
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR
HHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIAIKGTIKQAVMLGLAAT
CHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTAFWMFWRTWRGERNWLENM
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
HEHHHHDHEHHQDHDHDHDHDHDHDHEHHHHHEHGDNEEYQDAHARAHANDIKRRFDGRE
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
VTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLALTLVTVGVGAA
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR
HHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA