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The map label for this gene is ycjT [H]

Identifier: 209397081

GI number: 209397081

Start: 1852713

End: 1854980

Strand: Direct

Name: ycjT [H]

Synonym: ECH74115_1961

Alternate gene names: 209397081

Gene position: 1852713-1854980 (Clockwise)

Preceding gene: 209397813

Following gene: 209400484

Centisome position: 33.25

GC content: 54.1

Gene sequence:

>2268_bases
ATGACCAGGCCAGTAACGTTATCAGAACCCCATTTCAGCCAGCATACCCTGAACAAGTATGCATCGCTGATGGCGCAGGG
GAACGGCTATCTTGGGCTTCGCGCCAGCCATGAAGAAGATTACACGCGCCAGACGCGAGGGATGTATCTGGCGGGGCTGT
ATCATCGGGCGGGAAAAGGTGAAATCAACGAACTGGTGAACCTGCCTGATATCTTGGGGATGGAGATTGCCATAAATGGT
GAGGTTTTCTCGTTATCCCACGAAGCCTGGCAGCGTGAGCTTGACTTTGCCAGTGGCGAATTACGCCGCAACGTTGTCTG
GCGTACCAGCAACGGCGCAGGTTACACCATCGCCAGCCGTCGCTTTGTTTCGGCAGACCAACTGCCGCTCATTGCGCTGG
AAATCACTATTACGCCACTGGACGCCGACGCGTCAGTGCTGATTTCAACAGGTATCGACGCCACGCAAACCAACCACGGT
CGCCAACATCTCGACGAAACCCAGGTGCGGGTGTTTGGTCAGCATCTGATGCAGGGGATCTACACCACCCAGGATGGACG
CAGTGATGTGGCCATCAGCTGTTGCTGTAAGGTGAGCGGTGATGTGCAGCAATGCTATACCGCCAAAGAGCGCCGTTTGC
TGCAACATACCAGTGCGCAGCTTCATGCAGGCGAGACAGTGACGTTGCAAAAACTGGTGTGGATCGACTGGCGGGATGAC
AGGCAAGCCGTTTTAGACGAGTGGGGCAGCGCGTCGCTTCGCCAGCTTGAAATATGCGCGCAGCAGAGTTACGACCAACT
TCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGCGGCGATGCGCACGATC
AGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCGGCTCACGACGAGCGCAGCAGTATTGCGGCAAAA
GGCTTAACCGGCGAAGGCTACAAAGGCCACGTTTTCTGGGATACAGAAGTATTTTTGCTGCCGTTCCATCTGTTTAGCGA
TCCGACGGTTGCCCGAAGTTTACTGCGTTATCGCTGGCACAACTTGCCAGGCGCGCAGGAGAAAGCACGGCGCAGCGGCT
GGCAGGGCGCGCTATTTCCGTGGGAAAGCGCGCGCAGCGGCGAAGAAGAGACGCCAGAATTTGCCGCCATTAACATTCGT
ACCGGGCTGCGGCAAAAAGTGGCCTCGGCGCAGGCGGAACATCATCTGGTGGCCGATATCGCCTGGGCGGTTATTCAATA
CTGGCAGACCACGGGGGATGAAAGTTTCATTGCTCATGAAGGCATGGCGCTACTTCTGGAAACTGCAAAGTTCTGGATTA
GCCGCGCGGTGAGGGTTAACGACCGTCTGGAAATTCATGATGTTATTGGGCCAGACGAATATACCGAACATGTCAATAAT
AACGCCTTCACCAGCTATATGGCCCGCTACAACGTTCAACAGGCGCTGAATATTGCCCGCCAGTTCGGCTGTAGCGACGA
TGCGTTTATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAACGCAGCCCGATGGCGTTTTGCCGCAGG
ATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCTAAATACAAAGCGGCGGCGGGGAAGCAAACCATTCTGCTGGAT
TATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCA
GTTCTCAGCGGTATCGTGTCTTGCCAATCTGCAATTTTATGAACCGCGCACTATTCACGACTCGTCATTAAGTAAAGCAA
TCCACGGCATTGTTGCCGCACGCTGTGGCCTGCTGACCCAAAGTTATCAGTTCTGGCGCGAGGGGACTGAAATCGATCTT
GGTGCTGATCCGCATAGTTGTGATGATGGTATCCACGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTT
TGCCGGGGTGAGCGTGCGTGACGGTGAATTACATCTCAATCCGGCGTTACCGGAGCAGTGGCAACAGTTGTCTTTCCCTC
TGTTCTGGCAGGGCTGCGAATTACAGGTCACGCTCGACGCGCAGCGTATTGCGATTCGAACTTCTGCGCCCGTTTCACTG
CGTTTGAACGGGCAGCTTATATCCGTGGCTGAAGAATCTGTTTTCTGTTTGGGTGATTTTATTTTGCCCTTCAATGGGAC
CGCTACCACGCATCAGGAGGATGAATGA

Upstream 100 bases:

>100_bases
GCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGTACATCATCCAGCAACTGGTGGCGGCGTTATATCAATCCGCAGAA
ACAGGGACGCGTGTGGAATT

Downstream 100 bases:

>100_bases
AACTGCAAGGGGTAATTTTCGATCTGGATGGTGTAATCACCGATACCGCGCATCTGCATTTCCAGGCGTGGCAGCAGATT
GCCGCTGAAATTGGCATCAG

Product: glycosyl hydrolase, family 65

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 755; Mature: 754

Protein sequence:

>755_residues
MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQTRGMYLAGLYHRAGKGEINELVNLPDILGMEIAING
EVFSLSHEAWQRELDFASGELRRNVVWRTSNGAGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTSAQLHAGETVTLQKLVWIDWRDD
RQAVLDEWGSASLRQLEICAQQSYDQLLAASTENWRQWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAK
GLTGEGYKGHVFWDTEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRSGWQGALFPWESARSGEEETPEFAAINIR
TGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAKFWISRAVRVNDRLEIHDVIGPDEYTEHVNN
NAFTSYMARYNVQQALNIARQFGCSDDAFIHRAEMFLKELWMPETQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLD
YSRAEVNEMQILKQADVVMLNYMLPEQFSAVSCLANLQFYEPRTIHDSSLSKAIHGIVAARCGLLTQSYQFWREGTEIDL
GADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPALPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSL
RLNGQLISVAEESVFCLGDFILPFNGTATTHQEDE

Sequences:

>Translated_755_residues
MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQTRGMYLAGLYHRAGKGEINELVNLPDILGMEIAING
EVFSLSHEAWQRELDFASGELRRNVVWRTSNGAGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTSAQLHAGETVTLQKLVWIDWRDD
RQAVLDEWGSASLRQLEICAQQSYDQLLAASTENWRQWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAK
GLTGEGYKGHVFWDTEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRSGWQGALFPWESARSGEEETPEFAAINIR
TGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAKFWISRAVRVNDRLEIHDVIGPDEYTEHVNN
NAFTSYMARYNVQQALNIARQFGCSDDAFIHRAEMFLKELWMPETQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLD
YSRAEVNEMQILKQADVVMLNYMLPEQFSAVSCLANLQFYEPRTIHDSSLSKAIHGIVAARCGLLTQSYQFWREGTEIDL
GADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPALPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSL
RLNGQLISVAEESVFCLGDFILPFNGTATTHQEDE
>Mature_754_residues
TRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQTRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGE
VFSLSHEAWQRELDFASGELRRNVVWRTSNGAGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHGR
QHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTSAQLHAGETVTLQKLVWIDWRDDR
QAVLDEWGSASLRQLEICAQQSYDQLLAASTENWRQWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKG
LTGEGYKGHVFWDTEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRSGWQGALFPWESARSGEEETPEFAAINIRT
GLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAKFWISRAVRVNDRLEIHDVIGPDEYTEHVNNN
AFTSYMARYNVQQALNIARQFGCSDDAFIHRAEMFLKELWMPETQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDY
SRAEVNEMQILKQADVVMLNYMLPEQFSAVSCLANLQFYEPRTIHDSSLSKAIHGIVAARCGLLTQSYQFWREGTEIDLG
ADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPALPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLR
LNGQLISVAEESVFCLGDFILPFNGTATTHQEDE

Specific function: Unknown

COG id: COG1554

COG function: function code G; Trehalose and maltose hydrolases (possible phosphorylases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 65 family [H]

Homologues:

Organism=Homo sapiens, GI187829418, Length=354, Percent_Identity=28.2485875706215, Blast_Score=108, Evalue=3e-23,
Organism=Escherichia coli, GI1787575, Length=755, Percent_Identity=98.1456953642384, Blast_Score=1550, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6325283, Length=396, Percent_Identity=22.2222222222222, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24583760, Length=393, Percent_Identity=26.4631043256997, Blast_Score=105, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR011013
- InterPro:   IPR005194
- InterPro:   IPR005195
- InterPro:   IPR005196
- InterPro:   IPR017045 [H]

Pfam domain/function: PF03633 Glyco_hydro_65C; PF03632 Glyco_hydro_65m; PF03636 Glyco_hydro_65N [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 84802; Mature: 84671

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQTRGMYLAGLYHRAGKG
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCEEEEEHHHCCCCC
EINELVNLPDILGMEIAINGEVFSLSHEAWQRELDFASGELRRNVVWRTSNGAGYTIASR
CHHHHCCCCHHCCEEEEECCEEEEECHHHHHHHHCCCCCCCCCCEEEEECCCCCEEEECC
RFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHGRQHLDETQVRVFGQHLMQGI
EEECCCCCCEEEEEEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTSAQLHAGETVTLQKLVWIDWRDD
EECCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCC
RQAVLDEWGSASLRQLEICAQQSYDQLLAASTENWRQWWQKRRITVNGGDAHDQQALDYA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEECCCCCCHHHHHHHH
LYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWDTEVFLLPFHLFSDPTVARSLLRYRWH
HHEEEEECCCCCCCHHHHCCCCCCCCEEEEEEECCEEEEEEEHHCCCCHHHHHHHHHHHC
NLPGAQEKARRSGWQGALFPWESARSGEEETPEFAAINIRTGLRQKVASAQAEHHLVADI
CCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
AWAVIQYWQTTGDESFIAHEGMALLLETAKFWISRAVRVNDRLEIHDVIGPDEYTEHVNN
HHHHHHHHHCCCCCCEEHHCCHHHHHHHHHHHHHHHHEECCCEEEEECCCCHHHHHHCCC
NAFTSYMARYNVQQALNIARQFGCSDDAFIHRAEMFLKELWMPETQPDGVLPQDDSFMAK
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
PAINLAKYKAAAGKQTILLDYSRAEVNEMQILKQADVVMLNYMLPEQFSAVSCLANLQFY
CCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCEEC
EPRTIHDSSLSKAIHGIVAARCGLLTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIW
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHH
LGAIQGFAGVSVRDGELHLNPALPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSL
HHHHHCCCCCEEECCEEEECCCCCHHHHHCCCCEEECCCEEEEEECCEEEEEEECCCEEE
RLNGQLISVAEESVFCLGDFILPFNGTATTHQEDE
EECCEEEEEHHCCEEEEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
TRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQTRGMYLAGLYHRAGKG
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCEEEEEHHHCCCCC
EINELVNLPDILGMEIAINGEVFSLSHEAWQRELDFASGELRRNVVWRTSNGAGYTIASR
CHHHHCCCCHHCCEEEEECCEEEEECHHHHHHHHCCCCCCCCCCEEEEECCCCCEEEECC
RFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHGRQHLDETQVRVFGQHLMQGI
EEECCCCCCEEEEEEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTSAQLHAGETVTLQKLVWIDWRDD
EECCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCC
RQAVLDEWGSASLRQLEICAQQSYDQLLAASTENWRQWWQKRRITVNGGDAHDQQALDYA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEECCCCCCHHHHHHHH
LYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWDTEVFLLPFHLFSDPTVARSLLRYRWH
HHEEEEECCCCCCCHHHHCCCCCCCCEEEEEEECCEEEEEEEHHCCCCHHHHHHHHHHHC
NLPGAQEKARRSGWQGALFPWESARSGEEETPEFAAINIRTGLRQKVASAQAEHHLVADI
CCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
AWAVIQYWQTTGDESFIAHEGMALLLETAKFWISRAVRVNDRLEIHDVIGPDEYTEHVNN
HHHHHHHHHCCCCCCEEHHCCHHHHHHHHHHHHHHHHEECCCEEEEECCCCHHHHHHCCC
NAFTSYMARYNVQQALNIARQFGCSDDAFIHRAEMFLKELWMPETQPDGVLPQDDSFMAK
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
PAINLAKYKAAAGKQTILLDYSRAEVNEMQILKQADVVMLNYMLPEQFSAVSCLANLQFY
CCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCEEC
EPRTIHDSSLSKAIHGIVAARCGLLTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIW
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHH
LGAIQGFAGVSVRDGELHLNPALPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSL
HHHHHCCCCCEEECCEEEECCCCCHHHHHCCCCEEECCCEEEEEECCEEEEEEECCCEEE
RLNGQLISVAEESVFCLGDFILPFNGTATTHQEDE
EECCEEEEEHHCCEEEEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]