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The map label for this gene is lrhA

Identifier: 209396956

GI number: 209396956

Start: 3173235

End: 3174173

Strand: Reverse

Name: lrhA

Synonym: ECH74115_3428

Alternate gene names: 209396956

Gene position: 3174173-3173235 (Counterclockwise)

Preceding gene: 209398669

Following gene: 209400280

Centisome position: 56.97

GC content: 49.73

Gene sequence:

>939_bases
ATGATAAGTGCAAATCGTCCGATAATTAACCTCGACCTCGATCTGCTGAGAACATTTGTTGCTGTTGCCGATCTGAACAC
TTTTGCTGCCGCAGCTGCCGCTGTGTGTCGTACTCAGTCCGCCGTAAGTCAGCAAATGCAGCGTCTGGAACAACTCGTTG
GGAAAGAACTGTTCGCTCGTCACGGTCGCAACAAACTGTTAACTGAACATGGCATTCAACTTCTTGGTTACGCCAGGAAA
ATCCTGCGTTTTAATGATGAGGCCTGCTCATCATTAATGTTCAGTAATCTTCAGGGCGTGTTAACTATCGGTGCTTCAGA
TGAATCTGCCGATACGATCTTACCTTTCCTGTTAAATCGCGTGAGTTCGGTTTATCCGAAATTAGCGCTGGATGTCCGCG
TTAAACGTAATGCCTATATGGCAGAAATGCTGGAATCCCAGGAAGTGGATTTGATGGTAACCACCCATCGTCCTTCAGCG
TTTAAGGCATTGAACCTGCGTACCTCGCCAACACACTGGTACTGCGCAGCGGAATACGTATTGCAAAAAGGTGAGCCGAT
CCCTCTTGTATTGCTGGATGATCCAAGTCCGTTTCGCGATATGGTGCTGGCGACGCTTAATAAAGCAGATATTCCCTGGC
GACTGGCTTATGTCGCCTCGACGCTTCCGGCCGTTCGTGCGGCAGTGAAAGCCGGTCTTGGCGTGACGGCAAGGCCGGTC
GAGATGATGAGTCCGGACTTGCGTGTGCTGAGTGGAGTAGATGGCTTACCGCCGCTACCTGATACCGAATATTTGCTGTG
CTATGACCCGTCCAGTAATAATGAGCTGGCCCAGGTGATCTATCAGGCGATGGAGAGCTACCATAATCCGTGGCAATACA
GCCCAATATCTGCTCCGGAAGGGGATGATTCATTGTTGATTGAAAGGGATATCGAGTAA

Upstream 100 bases:

>100_bases
CGGATGAAACTGCCAGGGAGGCACATTACAAATGGAATTGCTTGTTTGTGTGTGCACAGCATTAACCAGCTCAGTATGAG
CCGCCAGTAAGTGATAATAT

Downstream 100 bases:

>100_bases
TCGACGGACGATAGATAATTCCTGAGACAATAGTGTAAAAAACGAGCCGCTGGGGGTGTTTTAAACCCCAGCGGCTTTTT
TTTAGTAAAGAACCTTCTTC

Product: transcriptional regulator LrhA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQQMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARK
ILRFNDEACSSLMFSNLQGVLTIGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYVASTLPAVRAAVKAGLGVTARPV
EMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNELAQVIYQAMESYHNPWQYSPISAPEGDDSLLIERDIE

Sequences:

>Translated_312_residues
MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQQMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARK
ILRFNDEACSSLMFSNLQGVLTIGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYVASTLPAVRAAVKAGLGVTARPV
EMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNELAQVIYQAMESYHNPWQYSPISAPEGDDSLLIERDIE
>Mature_312_residues
MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQQMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARK
ILRFNDEACSSLMFSNLQGVLTIGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYVASTLPAVRAAVKAGLGVTARPV
EMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNELAQVIYQAMESYHNPWQYSPISAPEGDDSLLIERDIE

Specific function: Not known, does not seem to act on the proton translocating NADH dehydrogenase genes (nuoA-N) which are part of the lrhA operon

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1788626, Length=312, Percent_Identity=99.3589743589744, Blast_Score=639, Evalue=0.0,
Organism=Escherichia coli, GI48994962, Length=151, Percent_Identity=30.4635761589404, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790399, Length=294, Percent_Identity=25.1700680272109, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787879, Length=133, Percent_Identity=36.0902255639098, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI145693105, Length=240, Percent_Identity=23.75, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787806, Length=130, Percent_Identity=30, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LRHA_ECO57 (Q8VWE6)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   E91025
- PIR:   F85869
- RefSeq:   NP_288863.1
- RefSeq:   NP_311200.1
- ProteinModelPortal:   Q8VWE6
- SMR:   Q8VWE6
- EnsemblBacteria:   EBESCT00000027576
- EnsemblBacteria:   EBESCT00000055377
- GeneID:   916881
- GeneID:   957618
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3549
- KEGG:   ecs:ECs3173
- GeneTree:   EBGT00050000008341
- HOGENOM:   HBG685425
- OMA:   PTLWYCA
- ProtClustDB:   PRK15092
- BioCyc:   ECOL83334:ECS3173-MONOMER
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00039

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 34562; Mature: 34562

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQQMQRLEQLVGKELFAR
CCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLTIGASDESADTILPFLLNR
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHH
VSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSAFKALNLRTSPTHWYCAAEYV
HHHHCCHHEEEEEEHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCCHHHHHHH
LQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYVASTLPAVRAAVKAGLGVTARPV
HHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCH
EMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNELAQVIYQAMESYHNPWQYSPISAPE
HHHCCCHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
GDDSLLIERDIE
CCCCEEEEECCC
>Mature Secondary Structure
MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQQMQRLEQLVGKELFAR
CCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLTIGASDESADTILPFLLNR
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHH
VSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSAFKALNLRTSPTHWYCAAEYV
HHHHCCHHEEEEEEHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCCHHHHHHH
LQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYVASTLPAVRAAVKAGLGVTARPV
HHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCH
EMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNELAQVIYQAMESYHNPWQYSPISAPE
HHHCCCHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
GDDSLLIERDIE
CCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796