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The map label for this gene is seqA [H]

Identifier: 209396469

GI number: 209396469

Start: 796286

End: 796831

Strand: Direct

Name: seqA [H]

Synonym: ECH74115_0782

Alternate gene names: 209396469

Gene position: 796286-796831 (Clockwise)

Preceding gene: 209397862

Following gene: 209398192

Centisome position: 14.29

GC content: 49.27

Gene sequence:

>546_bases
ATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGAGAGCGCATCCGA
CATTTTACGGCGTATGTTGAAATTTTCCGCCGCATCACAGCCTGCTGCTCCGGTGACGAAAGAGGTTCGCGTTGCGTCAC
CTGCTATCGTCGAAGCGAAGCCGATCAAAACGATTAAAGACAAGGTTCGCGCAATGCGTGAACTTCTGCTTTCGGATGAA
TATGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTACACTATATTCTCTTGACGCCCAGGCGTTTGC
CGAAGCAACGGAATCGTTGCACGGTCGTACACGCGTTTACTTTGCGGCAGATGAACAAACGCTGCTGAAAAATGGTAATC
AGACCAAGCCGAAACATGTGCCAGGCACGCCGTATTGGGTGATCACCAACACCAACACCGGCCGTAAATGCAGCATGATC
GAACACATCATGCAGTCGATGCAATTCCCGGCGGAATTGATTGAGAAGGTTTGCGGAACTATCTAA

Upstream 100 bases:

>100_bases
CGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTA
AGACACTGCACTGGATTAAG

Downstream 100 bases:

>100_bases
AACGTTGCAGACAAAGGACAAAGCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACG
TCGCCCAACTGACGGCGCAA

Product: replication initiation regulator SeqA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAPVTKEVRVASPAIVEAKPIKTIKDKVRAMRELLLSDE
YAEQKRAVNRFMLLLSTLYSLDAQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
EHIMQSMQFPAELIEKVCGTI

Sequences:

>Translated_181_residues
MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAPVTKEVRVASPAIVEAKPIKTIKDKVRAMRELLLSDE
YAEQKRAVNRFMLLLSTLYSLDAQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
EHIMQSMQFPAELIEKVCGTI
>Mature_181_residues
MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAPVTKEVRVASPAIVEAKPIKTIKDKVRAMRELLLSDE
YAEQKRAVNRFMLLLSTLYSLDAQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
EHIMQSMQFPAELIEKVCGTI

Specific function: Negative regulator of replication initiation. Required for sequestration, a process that blocks secondary initiation events. Could block the allosteric transition of dnaA from an inactive form to an active form. Binds hemimethylated oriC. This process has

COG id: COG3057

COG function: function code L; Negative regulator of replication initiationR

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786903, Length=181, Percent_Identity=99.4475138121547, Blast_Score=378, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005621
- InterPro:   IPR010985 [H]

Pfam domain/function: PF03925 SeqA [H]

EC number: NA

Molecular weight: Translated: 20330; Mature: 20330

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAPVTKEVRVASPAIVEAK
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCC
PIKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLDAQAFAEATESLHGRTRVY
CHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
FAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMIEHIMQSMQFPAELIEKVCGT
EECCHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHCC
I
C
>Mature Secondary Structure
MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAPVTKEVRVASPAIVEAK
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCC
PIKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLDAQAFAEATESLHGRTRVY
CHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
FAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMIEHIMQSMQFPAELIEKVCGT
EECCHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHCC
I
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]