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The map label for this gene is entE

Identifier: 209396204

GI number: 209396204

Start: 710815

End: 712425

Strand: Direct

Name: entE

Synonym: ECH74115_0679

Alternate gene names: 209396204

Gene position: 710815-712425 (Clockwise)

Preceding gene: 209397985

Following gene: 209396636

Centisome position: 12.76

GC content: 55.74

Gene sequence:

>1611_bases
ATGAGCATTCCATTCACCCGCTGGCCGGAAGAGTTTGCCCGTCGCTATCGGGAAAAAGGCTACTGGCAGGATTTGCCACT
GACTGACATTCTGACTCGCCACGCTGCGAGTGACAGCATCGCGGTTATCGACGGCGAGCGACAGTTGAGTTACCGGGAGC
TGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAACCGCGCTGGTACAACTG
GGTAACGTCGCTGAACTTTACATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCA
TCAGCGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTA
GCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCCATTCGCGTGGTGCAGCTACTCAACGACAGCGGTGAG
CATAACTTGCAGGATGCGATTAACCATCCGGCAGACGGTTTTACTGCTACGCCGTCACCTGCTGATGAAGTGGTCTATTT
CCAGCTTTCCGGCGGCACCACCGGTACACCGAAGCTGATCCCGCGCACTCATAACGACTACTACTACAGCGTGCGTCGTA
GCGTCGAGATTTGTCAGTTCACACAACAGACACGCTACCTGTGCGCGATCCCGGCGGCTCATAACTACGCCATGAGTTCG
CCGGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGATCCCAGCGCCACGCTTTGCTTCCCATT
GATTGAAAAACATCAGATTAACGTTACCGCGCTGGTGCCGCCGGCAGTCAGCCTGTGGTTGCAGGCGCTGACCGAAGGCG
AAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGTGGCGCACGTCTTTCTGCCACGCTTGCGGCGCGTATT
CCCGCTGAGATTGGTTGCCAGTTGCAGCAGGTGTTTGGTATGGCGGAAGGGCTGGTGAACTATACCCGTCTTGATGATAG
CGCGGAGAAAATTATCCATACCCAGGGTTACCCAATGTGTCCGGATGACGAAGTATGGGTTGCCGATGCCGAAGGAAATC
CACTGCCGCAAGGGGAAGTCGGACGACTGATGACGCGCGGGCCGTATACCTTCCGTGGCTATTACAAAAGCCCGCAGCAC
AATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGT
GCAGGGGCGCGAGAAAGATCAGATCAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGTTACTGCGCC
ATCCGGCGGTGATCTACGCCGCACTGGTCAGTATGGAAGATGAGCTGATGGGCGAAAAAAGCTGTGCTTATCTGGTGGTA
AAAGAGCCGCTTCGCGCGGTGCAGGTGCGTCGTTTCCTGCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGT
GGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAATTACGTCAGTGGCTGGCGTCACGCG
CATCAGCCTGA

Upstream 100 bases:

>100_bases
GGGGATTGTGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTG
GATTGCATTAAGGAGCGAGG

Downstream 100 bases:

>100_bases
AGGAGAGAACACGATGGCTATTCCAAAATTACAGGCTTACGCACTGCCGGAGTCTCACGATATTCCGCAGAATAAAGTTG
ACTGGGCCTTTGAACCGCAA

Product: enterobactin synthase subunit E

Products: NA

Alternate protein names: Enterochelin synthase E; 2,3-dihydroxybenzoate-AMP ligase; Dihydroxybenzoic acid-activating enzyme; S-dihydroxybenzoyltransferase

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDGERQLSYRELNQAADNLACSLRRQGIKPGETALVQL
GNVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
HNLQDAINHPADGFTATPSPADEVVYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEICQFTQQTRYLCAIPAAHNYAMSS
PGSLGVFLAGGTVVLAADPSATLCFPLIEKHQINVTALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARI
PAEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFRGYYKSPQH
NASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENLLLRHPAVIYAALVSMEDELMGEKSCAYLVV
KEPLRAVQVRRFLREQGIAEFKLPDRVECVDSLPLTAVGKVDKKQLRQWLASRASA

Sequences:

>Translated_536_residues
MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDGERQLSYRELNQAADNLACSLRRQGIKPGETALVQL
GNVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
HNLQDAINHPADGFTATPSPADEVVYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEICQFTQQTRYLCAIPAAHNYAMSS
PGSLGVFLAGGTVVLAADPSATLCFPLIEKHQINVTALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARI
PAEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFRGYYKSPQH
NASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENLLLRHPAVIYAALVSMEDELMGEKSCAYLVV
KEPLRAVQVRRFLREQGIAEFKLPDRVECVDSLPLTAVGKVDKKQLRQWLASRASA
>Mature_535_residues
SIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDGERQLSYRELNQAADNLACSLRRQGIKPGETALVQLG
NVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGEH
NLQDAINHPADGFTATPSPADEVVYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEICQFTQQTRYLCAIPAAHNYAMSSP
GSLGVFLAGGTVVLAADPSATLCFPLIEKHQINVTALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIP
AEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFRGYYKSPQHN
ASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENLLLRHPAVIYAALVSMEDELMGEKSCAYLVVK
EPLRAVQVRRFLREQGIAEFKLPDRVECVDSLPLTAVGKVDKKQLRQWLASRASA

Specific function: Activates the carboxylate group of 2,3-dihydroxy- benzoate (2,3-DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate. Then, catalyzes the acylation of holo-EntB with 2,3-DHB adenylate, preparing that molecule for amide bond formation with

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. EntE subfamily

Homologues:

Organism=Homo sapiens, GI38505220, Length=505, Percent_Identity=24.3564356435644, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI122937307, Length=510, Percent_Identity=23.7254901960784, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI42544132, Length=522, Percent_Identity=23.1800766283525, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI156151445, Length=358, Percent_Identity=25.4189944134078, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI157311624, Length=518, Percent_Identity=23.7451737451737, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI157311622, Length=518, Percent_Identity=23.7451737451737, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI58082049, Length=518, Percent_Identity=24.1312741312741, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI187761345, Length=378, Percent_Identity=25.1322751322751, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI187761343, Length=378, Percent_Identity=25.1322751322751, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI115511026, Length=511, Percent_Identity=22.7005870841487, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI28416953, Length=530, Percent_Identity=21.5094339622642, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI57165412, Length=382, Percent_Identity=23.8219895287958, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI57165410, Length=382, Percent_Identity=23.0366492146597, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI8923896, Length=547, Percent_Identity=20.109689213894, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI253970489, Length=380, Percent_Identity=21.8421052631579, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1786810, Length=536, Percent_Identity=98.8805970149254, Blast_Score=1097, Evalue=0.0,
Organism=Escherichia coli, GI145693145, Length=532, Percent_Identity=31.015037593985, Blast_Score=221, Evalue=6e-59,
Organism=Escherichia coli, GI221142682, Length=491, Percent_Identity=26.2729124236253, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1788107, Length=537, Percent_Identity=25.1396648044693, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1790505, Length=538, Percent_Identity=23.6059479553903, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1786801, Length=562, Percent_Identity=24.9110320284698, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1786529, Length=526, Percent_Identity=23.0038022813688, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1789201, Length=361, Percent_Identity=21.8836565096953, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788595, Length=507, Percent_Identity=23.2741617357002, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17557194, Length=539, Percent_Identity=26.530612244898, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17538037, Length=544, Percent_Identity=26.1029411764706, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI32563687, Length=504, Percent_Identity=23.6111111111111, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71994703, Length=521, Percent_Identity=23.8003838771593, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71994694, Length=519, Percent_Identity=23.8921001926782, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71994690, Length=521, Percent_Identity=23.8003838771593, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71996755, Length=525, Percent_Identity=24, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17560308, Length=363, Percent_Identity=26.7217630853994, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17558820, Length=504, Percent_Identity=24.2063492063492, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71985884, Length=358, Percent_Identity=27.6536312849162, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17531443, Length=369, Percent_Identity=23.5772357723577, Blast_Score=92, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17559526, Length=531, Percent_Identity=22.0338983050847, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17560140, Length=521, Percent_Identity=24.3761996161228, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71983001, Length=539, Percent_Identity=22.6345083487941, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71982997, Length=539, Percent_Identity=22.6345083487941, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=539, Percent_Identity=22.8200371057514, Blast_Score=124, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6319264, Length=540, Percent_Identity=22.037037037037, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24581924, Length=523, Percent_Identity=23.5181644359465, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI19922652, Length=497, Percent_Identity=23.5412474849095, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24656500, Length=530, Percent_Identity=23.9622641509434, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI21355181, Length=527, Percent_Identity=22.7703984819734, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356947, Length=388, Percent_Identity=24.4845360824742, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI18859661, Length=367, Percent_Identity=24.2506811989101, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648260, Length=272, Percent_Identity=26.8382352941176, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24667955, Length=567, Percent_Identity=21.1640211640212, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI62472339, Length=546, Percent_Identity=21.2454212454212, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24667959, Length=374, Percent_Identity=23.2620320855615, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21356441, Length=452, Percent_Identity=21.6814159292035, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24648253, Length=512, Percent_Identity=22.0703125, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24648255, Length=500, Percent_Identity=22.6, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI21358303, Length=499, Percent_Identity=22.6452905811623, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281365686, Length=329, Percent_Identity=25.8358662613982, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ENTE_ECO57 (Q8XBV3)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A99708
- PIR:   E85558
- RefSeq:   NP_286321.1
- RefSeq:   NP_308660.1
- ProteinModelPortal:   Q8XBV3
- SMR:   Q8XBV3
- EnsemblBacteria:   EBESCT00000025322
- EnsemblBacteria:   EBESCT00000055806
- GeneID:   916992
- GeneID:   957608
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0736
- KEGG:   ecs:ECs0633
- GeneTree:   EBGT00050000008803
- HOGENOM:   HBG547964
- OMA:   LHNASAF
- ProtClustDB:   PRK10946
- BioCyc:   ECOL83334:ECS0633-MONOMER
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963
- TIGRFAMs:   TIGR02275

Pfam domain/function: PF00501 AMP-binding

EC number: =2.7.7.58

Molecular weight: Translated: 59041; Mature: 58909

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDGERQLSYRELNQAADN
CCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHH
LACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIE
HHHHHHHCCCCCCCHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGEHNLQDAINHPADGFTATPSP
CEEEEECCCCCEECCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCCCCCC
ADEVVYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEICQFTQQTRYLCAIPAAHNYAMSS
CCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCC
PGSLGVFLAGGTVVLAADPSATLCFPLIEKHQINVTALVPPAVSLWLQALTEGESRAQLA
CCCEEEEEECCEEEEEECCCCEEEHHHHHCCCEEEEEECCHHHHHHHHHHHCCHHHHHHH
SLKLLQVGGARLSATLAARIPAEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMC
HHHHHHHCCCHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCCCC
PDDEVWVADAEGNPLPQGEVGRLMTRGPYTFRGYYKSPQHNASAFDANGFYCSGDLISID
CCCCEEEECCCCCCCCCCCHHHHHCCCCCEEECCCCCCCCCCCEECCCCEEECCCEEEEC
PEGYITVQGREKDQINRGGEKIAAEEIENLLLRHPAVIYAALVSMEDELMGEKSCAYLVV
CCCEEEEECCCHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEEEE
KEPLRAVQVRRFLREQGIAEFKLPDRVECVDSLPLTAVGKVDKKQLRQWLASRASA
HHHHHHHHHHHHHHHCCCEECCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDGERQLSYRELNQAADN
CCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHH
LACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIE
HHHHHHHCCCCCCCHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGEHNLQDAINHPADGFTATPSP
CEEEEECCCCCEECCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCCCCCC
ADEVVYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEICQFTQQTRYLCAIPAAHNYAMSS
CCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCC
PGSLGVFLAGGTVVLAADPSATLCFPLIEKHQINVTALVPPAVSLWLQALTEGESRAQLA
CCCEEEEEECCEEEEEECCCCEEEHHHHHCCCEEEEEECCHHHHHHHHHHHCCHHHHHHH
SLKLLQVGGARLSATLAARIPAEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMC
HHHHHHHCCCHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCCCC
PDDEVWVADAEGNPLPQGEVGRLMTRGPYTFRGYYKSPQHNASAFDANGFYCSGDLISID
CCCCEEEECCCCCCCCCCCHHHHHCCCCCEEECCCCCCCCCCCEECCCCEEECCCEEEEC
PEGYITVQGREKDQINRGGEKIAAEEIENLLLRHPAVIYAALVSMEDELMGEKSCAYLVV
CCCEEEEECCCHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEEEE
KEPLRAVQVRRFLREQGIAEFKLPDRVECVDSLPLTAVGKVDKKQLRQWLASRASA
HHHHHHHHHHHHHHHCCCEECCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796