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The map label for this gene is livM [H]

Identifier: 209396049

GI number: 209396049

Start: 4415768

End: 4417045

Strand: Reverse

Name: livM [H]

Synonym: ECH74115_4773

Alternate gene names: 209396049

Gene position: 4417045-4415768 (Counterclockwise)

Preceding gene: 209400625

Following gene: 209397425

Centisome position: 79.27

GC content: 56.65

Gene sequence:

>1278_bases
ATGAAACCGATGCATATTGCAATGGCGTTGCTCTCTGCCGCGATGTTTTTTGTCCTGGCGGGCGTCTTTATGGGCGTACA
ACTGGAGCTGGATGGCACCAAACTGGTGGTCGACACGGCTTCAGACATCCGTTGGCAGTGGGTGTTTATCGGCACGGCGG
TGGTCTTTTTCTTCCAGCTTTTGCGACCGGCTTTCCAGAAAGGGTTGAAAAGCGTTTCCGGACCGAAGTTTATTCTGCCC
GCCATTGATGGCTCCACGGTGAAGCAAAAGCTGTTTCTCGTGGCGCTGCTGGTGCTTGCGGTGGCGTGGCCGTTTATGGT
CTCACGCGGGACGGTGGATATCGCCACCCTGACCATGATCTACATTATCCTCGGACTCGGGCTGAACGTGGTGGTAGGGC
TTTCTGGTCTGCTGGTGCTGGGGTACGGCGGCTTTTACGCCATCGGCGCTTATACCTTTGCGCTGCTCAATCACTATTAC
GGCTTGGGCTTCTGGACCTGTCTGCCGATAGCCGGGTTGATGGCGGCGGCGGCGGGCTTCCTGCTCGGCTTCCCGGTGCT
TCGCTTGCGCGGTGACTATCTGGCGATCGTTACCCTCGGTTTCGGCGAAATTGTGCGCATATTGCTACTCAATAACACCG
AAATTACCGGCGGTCCGAACGGTATCAGTCAGATCCCGAAACCGACTTTTTTCGGCCTTGAGTTCAGCCGTACCGCTCGT
GAAGGCGGCTGGGACACGTTCAGTAATTTCTTTGGCCTGAAATACGATCCCTCCGATCGTGTCATTTTCCTCTACCTGGT
GGCGTTGCTGCTGGTGGTGCTAAGCCTGTTTGTCATTAACCGCCTGCTGCGGATGCCGCTGGGTCGCGCCTGGGAAGCGT
TGCGTGAAGATGAAATCGCCTGCCGTTCGCTGGGCTTAAGCCCGCGTCGTATCAAGCTGACTGCCTTTACCATCAGCGCC
GCGTTTGCCGGTTTTGCCGGAACGCTGTTTGCTGCGCGTCAGGGCTTTGTCAGCCCGGAATCCTTCACCTTTGCCGAATC
GGCGTTTGTGCTGGCGATAGTGGTGCTCGGCGGTATGGGCTCGCAATTTGCGGTAATTCTGGCGGCAATTTTGCTGGTGG
TGTCGCGCGAGTTGATGCGTGATTTCAACGAATACAGCATGTTAATGCTCGGTGGTTTGATGGTGCTGATGATGATCTGG
CGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAAAGGAGAGCAGGCATGA

Upstream 100 bases:

>100_bases
TGAGTACGGAATATAAAGATGTGGTCTCATTCGCCCTGCTGATTCTGGTGCTGCTGGTGATGCCGACCGGTATTCTGGGT
CGCCCGGAGGTAGAGAAAGT

Downstream 100 bases:

>100_bases
GTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCTTCGGCGGCCTGCTGGCTGTGAATAACGTCAATCTTGAATTG
TACCCGCAGGAGATCGTCTC

Product: leucine/isoleucine/valine transporter permease subunit

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIRWQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILP
AIDGSTVKQKLFLVALLVLAVAWPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGGPNGISQIPKPTFFGLEFSRTAR
EGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSLFVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISA
AFAGFAGTLFAARQGFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGGLMVLMMIW
RPQGLLPMTRPQLKLKNGAAKGEQA

Sequences:

>Translated_425_residues
MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIRWQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILP
AIDGSTVKQKLFLVALLVLAVAWPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGGPNGISQIPKPTFFGLEFSRTAR
EGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSLFVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISA
AFAGFAGTLFAARQGFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGGLMVLMMIW
RPQGLLPMTRPQLKLKNGAAKGEQA
>Mature_425_residues
MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIRWQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILP
AIDGSTVKQKLFLVALLVLAVAWPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGGPNGISQIPKPTFFGLEFSRTAR
EGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSLFVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISA
AFAGFAGTLFAARQGFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGGLMVLMMIW
RPQGLLPMTRPQLKLKNGAAKGEQA

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=425, Percent_Identity=99.5294117647059, Blast_Score=836, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 46318; Mature: 46318

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIRWQWVFIGTAVVFFFQL
CCHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCEEEEECCCCCEEEEEHHHHHHHHHHHH
LRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVAWPFMVSRGTVDIATLTMI
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
YIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYYGLGFWTCLPIAGLMAAAAGF
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGGPNGISQIPKPTFFGLEFSRTAR
HHHHHHHHHCCCEEEEEEECHHHHHHHHHCCCCCCCCCCCCHHHCCCCCEECCCHHHHHC
EGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSLFVINRLLRMPLGRAWEALREDEIA
CCCCHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
CRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQGFVSPESFTFAESAFVLAIVVLGGMG
HHHCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCC
SQFAVILAAILLVVSRELMRDFNEYSMLMLGGLMVLMMIWRPQGLLPMTRPQLKLKNGAA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCC
KGEQA
CCCCC
>Mature Secondary Structure
MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIRWQWVFIGTAVVFFFQL
CCHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCEEEEECCCCCEEEEEHHHHHHHHHHHH
LRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVAWPFMVSRGTVDIATLTMI
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
YIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYYGLGFWTCLPIAGLMAAAAGF
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGGPNGISQIPKPTFFGLEFSRTAR
HHHHHHHHHCCCEEEEEEECHHHHHHHHHCCCCCCCCCCCCHHHCCCCCEECCCHHHHHC
EGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSLFVINRLLRMPLGRAWEALREDEIA
CCCCHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
CRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQGFVSPESFTFAESAFVLAIVVLGGMG
HHHCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCC
SQFAVILAAILLVVSRELMRDFNEYSMLMLGGLMVLMMIWRPQGLLPMTRPQLKLKNGAA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCC
KGEQA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]