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The map label for this gene is gntR [H]

Identifier: 209396004

GI number: 209396004

Start: 4400065

End: 4401060

Strand: Reverse

Name: gntR [H]

Synonym: ECH74115_4754

Alternate gene names: 209396004

Gene position: 4401060-4400065 (Counterclockwise)

Preceding gene: 209400398

Following gene: 209396886

Centisome position: 78.98

GC content: 54.22

Gene sequence:

>996_bases
ATGAAAAAGAAAAGACCCGTACTTCAGGATGTGGCTGACCGTGTAGGCGTGACCAAAATGACGGTCAGCCGTTTTTTACG
CAACCCGGAGCAGGTTTCCGTCGCTCTACGCGGCAAGATTGCCGCGGCTCTTGATGAACTGGGCTATATTCCCAATCGTG
CGCCCGATATCCTCTCTAACGCCACCAGCCGGGCGATTGGCGTCCTGTTACCTTCTCTCACCAACCAGGTTTTCGCGGAA
GTATTACGCGGAATCGAAAGCGTCACCGACGCGCACGGTTATCAGACCATGCTGGCCCACTACGGTTATAAACCGGAAAT
GGAGCAAGAACGTCTCGAATCAATGCTCTCATGGAATATCGACGGACTGATCCTCACCGAACGTACCCACACGCCGCGCA
CCTTAAAGATGATTGAAGTGGCGGGGATCCCGGTGGTGGAGTTGATGGACAGCCAGTCGCCGTGTCTCGATATCGCCGTC
GGTTTTGATAACTTTGAAGCGGCACGCCAGATGACCACAGCCATTATTGCTCGCGGGCATCGCCACATTGCCTATCTCGG
CGCACGTCTCGACGAACGTACTATCATCAAACAGAAGGGATACGAACAGGCGATGCTGGATGCAGGCCTGGTGCCATATA
GCGTGATGGTTGAGCAATCTTCTTCTTACTCTTCCGGTATTGAACTGATTCGCCAGGCACGGCGGGAATATCCGCAGCTG
GATGGCGTGTTCTGTACTAATGATGATCTGGCGGTCGGCGCGGCGTTTGAATGTCAGCGTCTGGGGTTAAAAGTTCCTGA
CGATATGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGACTTGCGAGCGTGCTGACGCCGC
GTGAGCGGATGGGCAGTATTGGCGCTGAACGCCTGCTGGCGCGTATTCGTGGCGAATCTGTGACACCGAAAATGTTAGAT
TTAGGTTTCACCTTGTCACCGGGCGGATCTATTTAA

Upstream 100 bases:

>100_bases
GTATATCTAATAATTGTAAACAAGCACTGTTTTCCCCGGGAAGGTTCTGATGCGTCCGTGTTAAACTAAGAGAATCTATC
TCTTTTGTACCTTCAGGACG

Downstream 100 bases:

>100_bases
GCCCACAAATTTGAAGTAGCTCACACTTATACACTTAAGGCACGGATGGATATTGCTTCTGATATTGTCCGGCTGGACAA
TGTTACCGATAACAGTTACC

Product: transcriptional regulator GntR

Products: NA

Alternate protein names: Gluconate utilization system GNT-I transcriptional repressor [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAALDELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAE
VLRGIESVTDAHGYQTMLAHYGYKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVEQSSSYSSGIELIRQARREYPQL
DGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHGHDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLD
LGFTLSPGGSI

Sequences:

>Translated_331_residues
MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAALDELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAE
VLRGIESVTDAHGYQTMLAHYGYKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVEQSSSYSSGIELIRQARREYPQL
DGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHGHDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLD
LGFTLSPGGSI
>Mature_331_residues
MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAALDELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAE
VLRGIESVTDAHGYQTMLAHYGYKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVEQSSSYSSGIELIRQARREYPQL
DGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHGHDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLD
LGFTLSPGGSI

Specific function: Negative regulator for the gluconate utilization system GNT-I, the gntUKR operon [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI48994940, Length=331, Percent_Identity=99.6978851963746, Blast_Score=677, Evalue=0.0,
Organism=Escherichia coli, GI1790715, Length=332, Percent_Identity=46.3855421686747, Blast_Score=305, Evalue=2e-84,
Organism=Escherichia coli, GI1790369, Length=323, Percent_Identity=29.4117647058824, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1790194, Length=328, Percent_Identity=28.6585365853659, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1787948, Length=319, Percent_Identity=27.5862068965517, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787906, Length=330, Percent_Identity=27.8787878787879, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788474, Length=307, Percent_Identity=25.7328990228013, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1787580, Length=317, Percent_Identity=25.5520504731861, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789068, Length=317, Percent_Identity=25.2365930599369, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786540, Length=325, Percent_Identity=26.4615384615385, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1789202, Length=293, Percent_Identity=22.8668941979522, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1790689, Length=334, Percent_Identity=22.7544910179641, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36423; Mature: 36423

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAALDELGYIPNRAPDILSN
CCCCCCHHHHHHHHHCHHHHHHHHHHCCHHHEEHHHHHHHHHHHHHHCCCCCCCHHHHHH
ATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYGYKPEMEQERLESMLSWNI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
DGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAVGFDNFEAARQMTTAIIARGH
CEEEEECCCCCCCCEEEEEECCCCHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCC
RHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVEQSSSYSSGIELIRQARREYPQL
HHHHHHHCCCCHHHHHHHCCHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCC
DGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHGHDIGQVMEPRLASVLTPRERMGSI
CCEEECCCCCEECCHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCHHHHHCC
GAERLLARIRGESVTPKMLDLGFTLSPGGSI
CHHHHHHHHCCCCCCCHHHHCCEEECCCCCC
>Mature Secondary Structure
MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAALDELGYIPNRAPDILSN
CCCCCCHHHHHHHHHCHHHHHHHHHHCCHHHEEHHHHHHHHHHHHHHCCCCCCCHHHHHH
ATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYGYKPEMEQERLESMLSWNI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
DGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAVGFDNFEAARQMTTAIIARGH
CEEEEECCCCCCCCEEEEEECCCCHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCC
RHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVEQSSSYSSGIELIRQARREYPQL
HHHHHHHCCCCHHHHHHHCCHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCC
DGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHGHDIGQVMEPRLASVLTPRERMGSI
CCEEECCCCCEECCHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCHHHHHCC
GAERLLARIRGESVTPKMLDLGFTLSPGGSI
CHHHHHHHHCCCCCCCHHHHCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]