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The map label for this gene is yahI [H]

Identifier: 209395980

GI number: 209395980

Start: 395635

End: 396585

Strand: Direct

Name: yahI [H]

Synonym: ECH74115_0388

Alternate gene names: 209395980

Gene position: 395635-396585 (Clockwise)

Preceding gene: 209397696

Following gene: 209396661

Centisome position: 7.1

GC content: 56.68

Gene sequence:

>951_bases
ATGAAAGAACTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGC
GGAGGCGGTGAAAGCCGTGGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGTAACG
GGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACGAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAAC
TGTGTGGCAGATACGCAGGGCGGCATTGGCTACCTGATCCAACAGGCGCTGAACAACCGGCTGGCGCGTCACGGCGAGAA
AAAAGCCGTCACCGTGGTGACTCAGGTGGAAGTGGATAAAAACGATCCAGGTTTTGCCCATCCCACCAAGCCCATTGGTG
CATTCTTCAGTGAAAGCCAGCGTGACGAATTACAAAAGGCAAACCCTGACTGGCGTTTTGTTGAAGATGCTGGACGGGGC
TATCGCCGCGTGGTCGCCTCGCCGGAACCGAAACGTATTGTCGAAGCACCCGCCATTAAGGCGCTGATCCAACAAGGCTT
TGTGGTGATTGGCGCGGGCGGCGGCGGAATTCCTGTGGTGCGTACTGACGCGGGAGATTACCAAAGCGTGGACGCGGTTA
TCGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTGGTGATCACCACCGGCGTCGAAAAA
GTCTGTATCCACTTTGGTAAACCCGAGAAGCAGGCGCTGGATCGGGTGGATATTGCCACCATGACTCGCTATATGCAGGA
AGGACATTTCCCGCCCGGCAGCATGTTGCCAAAAATCATCGCCAGCCTGACGTTCCTGGAACAGGGCGGTAAAGAAGTGA
TTATCACCACGCCGGAATGCCTGCCAGCGGCGCTGCGCGGTGAAACGGGCACCCACATTATTAAAACGTAA

Upstream 100 bases:

>100_bases
CTACGAATGGCACGAGAATTTGTAGGCCTGGTAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCC
TCTGTAGCGGACATAACAAC

Downstream 100 bases:

>100_bases
GGACGTAAGATGAAAGAAAATAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCACCGCCGCCGCGCTGTT
TGGTACCTCTGTGCCGCTCG

Product: putative carbamate kinase

Products: ADP; carbamoyl phosphate

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASDYDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLAN
CVADTQGGIGYLIQQALNNRLARHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRG
YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDLSTALLAREIHADILVITTGVEK
VCIHFGKPEKQALDRVDIATMTRYMQEGHFPPGSMLPKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT

Sequences:

>Translated_316_residues
MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASDYDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLAN
CVADTQGGIGYLIQQALNNRLARHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRG
YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDLSTALLAREIHADILVITTGVEK
VCIHFGKPEKQALDRVDIATMTRYMQEGHFPPGSMLPKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT
>Mature_316_residues
MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASDYDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLAN
CVADTQGGIGYLIQQALNNRLARHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRG
YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDLSTALLAREIHADILVITTGVEK
VCIHFGKPEKQALDRVDIATMTRYMQEGHFPPGSMLPKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT

Specific function: Unknown

COG id: COG0549

COG function: function code E; Carbamate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbamate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1786516, Length=316, Percent_Identity=98.4177215189873, Blast_Score=634, Evalue=0.0,
Organism=Escherichia coli, GI1789238, Length=318, Percent_Identity=45.5974842767296, Blast_Score=258, Evalue=3e-70,
Organism=Escherichia coli, GI1786732, Length=313, Percent_Identity=37.6996805111821, Blast_Score=173, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR003964 [H]

Pfam domain/function: PF00696 AA_kinase [H]

EC number: 2.7.2.2

Molecular weight: Translated: 34000; Mature: 34000

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASDYDIVLTHGNGPQVGLD
CCCEEEEEECCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHH
LRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLARHGEKKAVTVVTQVEVDK
HHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECC
NDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRGYRRVVASPEPKRIVEAPAIK
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCEEECCCCCCHHHHHHHHHH
ALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDLSTALLAREIHADILVITTGVEK
HHHHCCCEEEEECCCCCCEEEECCCCCHHHHHHHHCCHHHHHHHHHHCCCEEEEECCHHH
VCIHFGKPEKQALDRVDIATMTRYMQEGHFPPGSMLPKIIASLTFLEQGGKEVIITTPEC
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCHH
LPAALRGETGTHIIKT
HHHHHCCCCCCEEECC
>Mature Secondary Structure
MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASDYDIVLTHGNGPQVGLD
CCCEEEEEECCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHH
LRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLARHGEKKAVTVVTQVEVDK
HHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECC
NDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRGYRRVVASPEPKRIVEAPAIK
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCEEECCCCCCHHHHHHHHHH
ALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDLSTALLAREIHADILVITTGVEK
HHHHCCCEEEEECCCCCCEEEECCCCCHHHHHHHHCCHHHHHHHHHHCCCEEEEECCHHH
VCIHFGKPEKQALDRVDIATMTRYMQEGHFPPGSMLPKIIASLTFLEQGGKEVIITTPEC
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCHH
LPAALRGETGTHIIKT
HHHHHCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; NH3; CO2

Specific reaction: ATP + NH3 + CO2 = ADP + carbamoyl phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]