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The map label for this gene is fliF [H]

Identifier: 209395809

GI number: 209395809

Start: 2545324

End: 2546982

Strand: Direct

Name: fliF [H]

Synonym: ECH74115_2714

Alternate gene names: 209395809

Gene position: 2545324-2546982 (Clockwise)

Preceding gene: 209399338

Following gene: 209396112

Centisome position: 45.68

GC content: 54.55

Gene sequence:

>1659_bases
ATGAATGCGACTGCAGCCCAGACAAAATCTCTTGAGTGGCTTAATCGCCTGCGTGCGAATCCGAAAATTCCATTGATTGT
TGCCGGTTCCGCGGCAGTGGCGGTCATGGTCGCACTGATCCTGTGGGCGAAAGCCCCCGACTACCGCACATTATTCAGCA
ATCTTTCCGATCAGGATGGTGGCGCAATTGTCAGCCAACTGACGCAAATGAATATTCCTTACCGCTTCAGCGAAGCCAGC
GGCGCTATTGAAGTTCCGGCAGATAAAGTTCACGAGCTGCGCCTGCGCCTGGCGCAACAAGGTTTGCCCAAAGGCGGCGC
GGTCGGTTTCGAACTACTCGATCAGGAAAAGTTTGGTATCAGCCAGTTCAGCGAACAGGTGAATTATCAGCGGGCGCTGG
AAGGCGAGCTTTCTCGTACCATCGAAACTATCGGCCCGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCT
TTATTCGTCCGTGAACAAAAATCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCA
AATTAGCGCCATTGTGCATCTGGTTTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCG
GACATCTGTTAACCCAGTCCAATACCAGCTGGCGCGATCTTAATGACGCTCAATTGAAATATGCCAGCGATGTCGAAGGC
CGTATTCAGCGGCGTATTGAAGCAATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTCACGGCGCAACT
GGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAATGGTGATGAATCTCAGGCGGCGCTTCGTTCACGCC
AGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCTCCTGCG
AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCC
GCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACTATTCGTCATACCAAAATGAACGTGGGCGATG
TGCAACGTCTGTCAGTCGCGGTAGTGGTGAATTACAAAACCTTGCCAGACGGTAAACCATTGCCTCTCAGCAACGAACAG
ATGAAACAAATTGAAGATCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGACTCGCTCAATGTCGTTAACTC
GCCGTTCAATAGTAGTGACGAAAGCGGCGGAGAACTGCCGTTCTGGCAACAGCAAGCGTTTATCGATCAGTTACTGGCTG
CCGGTCGCTGGTTGCTGGTACTGCTGGTGGCGTGGCTGCTGTGGCGTAAAGCGGTACGCCCGCAGCTAACACGTCGCGCT
GAGGCGATGAAAGCTGTACAGCAACAGGCGCAGGCCCGCGAGGAAGTGGAAGATGCGGTGGAAGTCCGCCTGAGCAAAGA
CGAACAACTCCAACAACGGCGCGCTAACCAACGTCTGGGGGCAGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATA
ACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAA

Upstream 100 bases:

>100_bases
CAAATAATGGGAGCGTCAATTTTTCGAGTTTGCTGACCCGGGAGTGAGTCTTGTTCCACTTTGCCAATAACGCCGTCCAT
AATCAGCCACGAGGTGCGCG

Downstream 100 bases:

>100_bases
CCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAAGTGTTCAAGCACCTCT
CCCAGCGCGAGGTGCAAACC

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAKAPDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEAS
GAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLTQSNTSWRDLNDAQLKYASDVEG
RIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTEEQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPA
NNAPISTPPANQNNRQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGKPLPLSNEQ
MKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQLLAAGRWLLVLLVAWLLWRKAVRPQLTRRA
EAMKAVQQQAQAREEVEDAVEVRLSKDEQLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE

Sequences:

>Translated_552_residues
MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAKAPDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEAS
GAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLTQSNTSWRDLNDAQLKYASDVEG
RIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTEEQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPA
NNAPISTPPANQNNRQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGKPLPLSNEQ
MKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQLLAAGRWLLVLLVAWLLWRKAVRPQLTRRA
EAMKAVQQQAQAREEVEDAVEVRLSKDEQLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE
>Mature_552_residues
MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAKAPDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEAS
GAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLTQSNTSWRDLNDAQLKYASDVEG
RIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTEEQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPA
NNAPISTPPANQNNRQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGKPLPLSNEQ
MKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQLLAAGRWLLVLLVAWLLWRKAVRPQLTRRA
EAMKAVQQQAQAREEVEDAVEVRLSKDEQLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=552, Percent_Identity=99.6376811594203, Blast_Score=1113, Evalue=0.0,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 60710; Mature: 60710

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAKAPDYRTLFSNLSDQDG
CCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCC
GAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEKFGI
HHHHHHHHHCCCCEEECCCCCCEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHCCH
SQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPSLFVREQKSPSASVTVNLLPG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCCCCEEEEEECCC
RALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLTQSNTSWRDLNDAQLKYASDVEG
CCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCCCCCCCHHHHHHHCCHHH
RIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTEEQYRPNGDESQAALRSRQLNESEQ
HHHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHCCCHHH
SGSGYPGGVPGALSNQPAPANNAPISTPPANQNNRQQQASTTSNSGPRSTQRNETSNYEV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCH
DRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGKPLPLSNEQMKQIEDLTREAMGFSEKRGD
HHHHHHHHCCCCCHHEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCHHHCCC
SLNVVNSPFNSSDESGGELPFWQQQAFIDQLLAAGRWLLVLLVAWLLWRKAVRPQLTRRA
CCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
EAMKAVQQQAQAREEVEDAVEVRLSKDEQLQQRRANQRLGAEVMSQRIREMSDNDPRVVA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LVIRQWINNDHE
HHHHHHHCCCCC
>Mature Secondary Structure
MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAKAPDYRTLFSNLSDQDG
CCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCC
GAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEKFGI
HHHHHHHHHCCCCEEECCCCCCEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHCCH
SQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPSLFVREQKSPSASVTVNLLPG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCCCCEEEEEECCC
RALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLTQSNTSWRDLNDAQLKYASDVEG
CCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCCCCCCCHHHHHHHCCHHH
RIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTEEQYRPNGDESQAALRSRQLNESEQ
HHHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHCCCHHH
SGSGYPGGVPGALSNQPAPANNAPISTPPANQNNRQQQASTTSNSGPRSTQRNETSNYEV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCH
DRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGKPLPLSNEQMKQIEDLTREAMGFSEKRGD
HHHHHHHHCCCCCHHEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCHHHCCC
SLNVVNSPFNSSDESGGELPFWQQQAFIDQLLAAGRWLLVLLVAWLLWRKAVRPQLTRRA
CCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
EAMKAVQQQAQAREEVEDAVEVRLSKDEQLQQRRANQRLGAEVMSQRIREMSDNDPRVVA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LVIRQWINNDHE
HHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1551848; 8224881 [H]