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The map label for this gene is gidA

Identifier: 209696454

GI number: 209696454

Start: 3323167

End: 3325056

Strand: Reverse

Name: gidA

Synonym: VSAL_I3070

Alternate gene names: 209696454

Gene position: 3325056-3323167 (Counterclockwise)

Preceding gene: NA

Following gene: 209696453

Centisome position: 100.0

GC content: 39.68

Gene sequence:

>1890_bases
ATGTTTTACCAAGAAAACTTTGATGTCATTGTTATTGGCGGTGGTCATGCTGGTACAGAGGCTGCACTTGCTGCAGCTAG
AACCGGACAAAAGACACTCTTACTTACTCATAACATTGATACATTAGGTCAAATGTCGTGTAACCCAGCAATTGGTGGGA
TCGGCAAGGGCCATTTGGTAAAAGAAGTCGATGCTTTAGGCGGGTTAATGGCTGAAGCCATTGATCACAGTGGTATACAA
TTTAGAACACTGAATGCATCGAAAGGACCAGCAGTTCGAGCAACTCGTGCTCAAGCCGATCGCGCGTTATACAAAACCTA
CGTACGTTCTGTATTAGAAAATCAACCAAACTTAACCTTGTTTCAGCAAGCGGTAGACGATTTGATCATTGAAAATGATA
AAGTTATCGGTGCTGTCACTCAAATGGGCCTTAAATTCCGTGCTAAATCAGTTGTACTGACTGCTGGCACATTTTTAGGG
GGTCAGATACATATCGGAATGGAAAACTTCTCAGGAGGCCGTGCAGGGGATCCTTCTTCGATCACTCTTGCTCAACGTTT
AAGAGATCGTCCGTTTAGAATAGATCGTCTGAAAACAGGTACTCCACCTCGAATTGATGCGAGAAGTGTTGATTTCTCCA
ATTTAGAAGCACAACCCGGTGATAATCCAACCCCTGTATTTTCCTTTTTAGGTAACCGTCAACAACATCCACAACAAGTA
AATTGTTTTATTACTCACACTAATGAAAAAACGCATGATGTCATTCGTAAAAATTTAGACCGTAGCCCTATGTATGCGGG
AGTTATTGAAGGTATTGGTCCACGTTACTGTCCTTCAATTGAAGATAAAGTAATGCGTTTTGCAGATAAAGACAGTCACC
AAATATTCATTGAGCCAGAAGGACTAACAACCCACGAATTGTACCCGAATGGCATTTCAACAAGCCTTCCTTTTGACGTT
CAATTACAAATTGTTCGTTCAATGAAAGGATTCGAAAATGCACACATTGTGCGTCCAGGTTATGCGATAGAATACGATTT
CTTTGATCCTCGAGATTTAAAAGCAACGTATGAAACGAAGTTTATTGAAGGCTTATTCTTTGCTGGTCAAATAAACGGAA
CGACCGGTTATGAAGAAGCGGCTGCGCAAGGCTTAATGGCTGGTTTAAATGCAAGCTTATTCTCTCAAGGAAAAGAAGGC
TGGAGCCCGCGTCGTGATGAAGCCTACATGGGCGTATTAATTGATGATTTATCAACGCTTGGCACAAAAGAACCTTACAG
AATGTTCACTTCTCGTGCTGAATACCGTTTGTCATTACGTGAAGATAACGCTGATTTACGCTTAACTGAACAGGGTCGAG
AACTTGGACTTGTTGATGATATTCGTTGGTCTCGTTTTAATGAAAAAGTAGAAAATATTGAAAAAGAACGCCAACGTTTA
AAAGATATTTGGATCAATCCAACGTCTGAAAATATAGATCAAGTTAACTCGATCTTAAAAACACCGATCGTTCGTGAAGC
AAGTGGTGAGGATCTTCTTCGTCGCCCAGAAGTTACGTATTCATCATTAATGACAATTGAAAAATTTGGTCCTGCTAGTG
ATGATCAACAAGCGTCTGAACAAGTTGAAATTCAAACTAAATACGCGGGTTATATTCAACGTCAACGTGATGAAATTGAA
AAATCATTACGTCATGAAAATACAAAATTGCCGTTTGATTTGGAATACAACAAAGTTAAAGGCCTTTCAAATGAAGTGGT
CGCAAAATTAAGCGACGCAAAACCTGAAAGTATTGGTATAGCATCTCGAATTTCAGGAATTACACCGGCTGCGATTTCAA
TTTTGTTAGTTCATTTAAAAAAACATGGTTTATTGAAGAAAGGTAAGTAA

Upstream 100 bases:

>100_bases
AACTTCATCATTTTTTCATTTCTCTCAAGCCTAGAAAAAGGGTAAAATACTCCTCCTTGATTAAATTCATCCAACTCTTT
AAAACACCTAAGGTCTAATC

Downstream 100 bases:

>100_bases
CGTGACCGATTTATCTCAACAATTCGATCGTTTGATTGCTCAAACTGATTTAACTGTCTCTGAATTGCAAAAAGAACAAT
TACTTGGTTATGTCGCCCTA

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 629; Mature: 629

Protein sequence:

>629_residues
MFYQENFDVIVIGGGHAGTEAALAAARTGQKTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEVDALGGLMAEAIDHSGIQ
FRTLNASKGPAVRATRAQADRALYKTYVRSVLENQPNLTLFQQAVDDLIIENDKVIGAVTQMGLKFRAKSVVLTAGTFLG
GQIHIGMENFSGGRAGDPSSITLAQRLRDRPFRIDRLKTGTPPRIDARSVDFSNLEAQPGDNPTPVFSFLGNRQQHPQQV
NCFITHTNEKTHDVIRKNLDRSPMYAGVIEGIGPRYCPSIEDKVMRFADKDSHQIFIEPEGLTTHELYPNGISTSLPFDV
QLQIVRSMKGFENAHIVRPGYAIEYDFFDPRDLKATYETKFIEGLFFAGQINGTTGYEEAAAQGLMAGLNASLFSQGKEG
WSPRRDEAYMGVLIDDLSTLGTKEPYRMFTSRAEYRLSLREDNADLRLTEQGRELGLVDDIRWSRFNEKVENIEKERQRL
KDIWINPTSENIDQVNSILKTPIVREASGEDLLRRPEVTYSSLMTIEKFGPASDDQQASEQVEIQTKYAGYIQRQRDEIE
KSLRHENTKLPFDLEYNKVKGLSNEVVAKLSDAKPESIGIASRISGITPAAISILLVHLKKHGLLKKGK

Sequences:

>Translated_629_residues
MFYQENFDVIVIGGGHAGTEAALAAARTGQKTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEVDALGGLMAEAIDHSGIQ
FRTLNASKGPAVRATRAQADRALYKTYVRSVLENQPNLTLFQQAVDDLIIENDKVIGAVTQMGLKFRAKSVVLTAGTFLG
GQIHIGMENFSGGRAGDPSSITLAQRLRDRPFRIDRLKTGTPPRIDARSVDFSNLEAQPGDNPTPVFSFLGNRQQHPQQV
NCFITHTNEKTHDVIRKNLDRSPMYAGVIEGIGPRYCPSIEDKVMRFADKDSHQIFIEPEGLTTHELYPNGISTSLPFDV
QLQIVRSMKGFENAHIVRPGYAIEYDFFDPRDLKATYETKFIEGLFFAGQINGTTGYEEAAAQGLMAGLNASLFSQGKEG
WSPRRDEAYMGVLIDDLSTLGTKEPYRMFTSRAEYRLSLREDNADLRLTEQGRELGLVDDIRWSRFNEKVENIEKERQRL
KDIWINPTSENIDQVNSILKTPIVREASGEDLLRRPEVTYSSLMTIEKFGPASDDQQASEQVEIQTKYAGYIQRQRDEIE
KSLRHENTKLPFDLEYNKVKGLSNEVVAKLSDAKPESIGIASRISGITPAAISILLVHLKKHGLLKKGK
>Mature_629_residues
MFYQENFDVIVIGGGHAGTEAALAAARTGQKTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEVDALGGLMAEAIDHSGIQ
FRTLNASKGPAVRATRAQADRALYKTYVRSVLENQPNLTLFQQAVDDLIIENDKVIGAVTQMGLKFRAKSVVLTAGTFLG
GQIHIGMENFSGGRAGDPSSITLAQRLRDRPFRIDRLKTGTPPRIDARSVDFSNLEAQPGDNPTPVFSFLGNRQQHPQQV
NCFITHTNEKTHDVIRKNLDRSPMYAGVIEGIGPRYCPSIEDKVMRFADKDSHQIFIEPEGLTTHELYPNGISTSLPFDV
QLQIVRSMKGFENAHIVRPGYAIEYDFFDPRDLKATYETKFIEGLFFAGQINGTTGYEEAAAQGLMAGLNASLFSQGKEG
WSPRRDEAYMGVLIDDLSTLGTKEPYRMFTSRAEYRLSLREDNADLRLTEQGRELGLVDDIRWSRFNEKVENIEKERQRL
KDIWINPTSENIDQVNSILKTPIVREASGEDLLRRPEVTYSSLMTIEKFGPASDDQQASEQVEIQTKYAGYIQRQRDEIE
KSLRHENTKLPFDLEYNKVKGLSNEVVAKLSDAKPESIGIASRISGITPAAISILLVHLKKHGLLKKGK

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=631, Percent_Identity=46.2757527733756, Blast_Score=504, Evalue=1e-142,
Organism=Homo sapiens, GI19882217, Length=656, Percent_Identity=44.5121951219512, Blast_Score=489, Evalue=1e-138,
Organism=Homo sapiens, GI183227703, Length=671, Percent_Identity=43.5171385991058, Blast_Score=484, Evalue=1e-136,
Organism=Escherichia coli, GI2367273, Length=627, Percent_Identity=77.8309409888357, Blast_Score=1027, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=633, Percent_Identity=42.654028436019, Blast_Score=491, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6321202, Length=623, Percent_Identity=44.6227929373997, Blast_Score=497, Evalue=1e-141,
Organism=Drosophila melanogaster, GI24658174, Length=636, Percent_Identity=46.0691823899371, Blast_Score=514, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_ALISL (B6EHU8)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002264385.1
- ProteinModelPortal:   B6EHU8
- GeneID:   6988589
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I3070
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 69983; Mature: 69983

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: BINDING 125-125 BINDING 180-180 BINDING 370-370

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYQENFDVIVIGGGHAGTEAALAAARTGQKTLLLTHNIDTLGQMSCNPAIGGIGKGHLV
CCCCCCCCEEEEECCCCCHHHHHHHHCCCCEEEEEEECCCCHHCCCCCCCCCCCCCCHHH
KEVDALGGLMAEAIDHSGIQFRTLNASKGPAVRATRAQADRALYKTYVRSVLENQPNLTL
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCHHH
FQQAVDDLIIENDKVIGAVTQMGLKFRAKSVVLTAGTFLGGQIHIGMENFSGGRAGDPSS
HHHHHHHHHCCCCCEEHHHHHHCHHEEECEEEEEECEEECCEEEEEEECCCCCCCCCCCH
ITLAQRLRDRPFRIDRLKTGTPPRIDARSVDFSNLEAQPGDNPTPVFSFLGNRQQHPQQV
HHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCEE
NCFITHTNEKTHDVIRKNLDRSPMYAGVIEGIGPRYCPSIEDKVMRFADKDSHQIFIEPE
EEEEEECCCHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEECC
GLTTHELYPNGISTSLPFDVQLQIVRSMKGFENAHIVRPGYAIEYDFFDPRDLKATYETK
CCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCEEEEECCCCCCCCCHHHHHH
FIEGLFFAGQINGTTGYEEAAAQGLMAGLNASLFSQGKEGWSPRRDEAYMGVLIDDLSTL
HHHHEEEEEEECCCCCHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
GTKEPYRMFTSRAEYRLSLREDNADLRLTEQGRELGLVDDIRWSRFNEKVENIEKERQRL
CCCCHHHHHHCCCCEEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KDIWINPTSENIDQVNSILKTPIVREASGEDLLRRPEVTYSSLMTIEKFGPASDDQQASE
HHHCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHCCCCCHHHHHHHHHCCCCCCCHHHHH
QVEIQTKYAGYIQRQRDEIEKSLRHENTKLPFDLEYNKVKGLSNEVVAKLSDAKPESIGI
HEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHCCCCHHHHHHHCCCCCCCCCH
ASRISGITPAAISILLVHLKKHGLLKKGK
HHHHCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MFYQENFDVIVIGGGHAGTEAALAAARTGQKTLLLTHNIDTLGQMSCNPAIGGIGKGHLV
CCCCCCCCEEEEECCCCCHHHHHHHHCCCCEEEEEEECCCCHHCCCCCCCCCCCCCCHHH
KEVDALGGLMAEAIDHSGIQFRTLNASKGPAVRATRAQADRALYKTYVRSVLENQPNLTL
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCHHH
FQQAVDDLIIENDKVIGAVTQMGLKFRAKSVVLTAGTFLGGQIHIGMENFSGGRAGDPSS
HHHHHHHHHCCCCCEEHHHHHHCHHEEECEEEEEECEEECCEEEEEEECCCCCCCCCCCH
ITLAQRLRDRPFRIDRLKTGTPPRIDARSVDFSNLEAQPGDNPTPVFSFLGNRQQHPQQV
HHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCEE
NCFITHTNEKTHDVIRKNLDRSPMYAGVIEGIGPRYCPSIEDKVMRFADKDSHQIFIEPE
EEEEEECCCHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEECC
GLTTHELYPNGISTSLPFDVQLQIVRSMKGFENAHIVRPGYAIEYDFFDPRDLKATYETK
CCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCEEEEECCCCCCCCCHHHHHH
FIEGLFFAGQINGTTGYEEAAAQGLMAGLNASLFSQGKEGWSPRRDEAYMGVLIDDLSTL
HHHHEEEEEEECCCCCHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
GTKEPYRMFTSRAEYRLSLREDNADLRLTEQGRELGLVDDIRWSRFNEKVENIEKERQRL
CCCCHHHHHHCCCCEEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KDIWINPTSENIDQVNSILKTPIVREASGEDLLRRPEVTYSSLMTIEKFGPASDDQQASE
HHHCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHCCCCCHHHHHHHHHCCCCCCCHHHHH
QVEIQTKYAGYIQRQRDEIEKSLRHENTKLPFDLEYNKVKGLSNEVVAKLSDAKPESIGI
HEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHCCCCHHHHHHHCCCCCCCCCH
ASRISGITPAAISILLVHLKKHGLLKKGK
HHHHCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA