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The map label for this gene is gidB [H]

Identifier: 209696453

GI number: 209696453

Start: 3322536

End: 3323165

Strand: Reverse

Name: gidB [H]

Synonym: VSAL_I3069

Alternate gene names: 209696453

Gene position: 3323165-3322536 (Counterclockwise)

Preceding gene: 209696454

Following gene: 209696452

Centisome position: 99.94

GC content: 38.1

Gene sequence:

>630_bases
GTGACCGATTTATCTCAACAATTCGATCGTTTGATTGCTCAAACTGATTTAACTGTCTCTGAATTGCAAAAAGAACAATT
ACTTGGTTATGTCGCCCTACTTCATAAGTGGAATAAAGCGTATAACTTAACCTCAGTAAGAAACCCAAGTGAAATGGTAA
TTAAACATATCTTGGATAGTATTGTTGTTAGTCCAAATCTATCTGGTGAGCGATTTATTGATGTTGGTACCGGTCCTGGT
TTACCAGGGATACCATTAGCTATCATGAATCCTGAAAAGTCATTTACACTGCTCGATAGCTTAGGAAAGCGAATTCGCTT
TATTAAACAAGTGATTCATGAATTAAAAATAACCAATGTCACTCCGATTCAAAGTCGTGTTGAAGACTTCCAACCGGAAG
AAAAGTTTGATGCAGTCCTAAGTCGTGCGTTTGCGTCAATGACAGACATGGTGAATTGGTGTCATCATCTACCAAAAGAA
GATGGTGTGTTTTTGGCATTAAAAGGGATCTACTCAGAAGAAGAAGCAAATGATCTTCCTGAATGGTGTTCAGTCAGAGA
TGTAGTGACATTGATCGTTCCGGAGTTAGAGGGTGAAAGACACTTAGTCACCTTAACCGGAAAAAAATAA

Upstream 100 bases:

>100_bases
ATTGGTATAGCATCTCGAATTTCAGGAATTACACCGGCTGCGATTTCAATTTTGTTAGTTCATTTAAAAAAACATGGTTT
ATTGAAGAAAGGTAAGTAAC

Downstream 100 bases:

>100_bases
TGGAATGAGGTTAATGTGGGAAGAGTTATCTCTATTGCGAACCAAAAAGGTGGCGTAGGCAAAACAACAACGGCGGTGAA
CCTTGCCGCTTCAATGGCTG

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MTDLSQQFDRLIAQTDLTVSELQKEQLLGYVALLHKWNKAYNLTSVRNPSEMVIKHILDSIVVSPNLSGERFIDVGTGPG
LPGIPLAIMNPEKSFTLLDSLGKRIRFIKQVIHELKITNVTPIQSRVEDFQPEEKFDAVLSRAFASMTDMVNWCHHLPKE
DGVFLALKGIYSEEEANDLPEWCSVRDVVTLIVPELEGERHLVTLTGKK

Sequences:

>Translated_209_residues
MTDLSQQFDRLIAQTDLTVSELQKEQLLGYVALLHKWNKAYNLTSVRNPSEMVIKHILDSIVVSPNLSGERFIDVGTGPG
LPGIPLAIMNPEKSFTLLDSLGKRIRFIKQVIHELKITNVTPIQSRVEDFQPEEKFDAVLSRAFASMTDMVNWCHHLPKE
DGVFLALKGIYSEEEANDLPEWCSVRDVVTLIVPELEGERHLVTLTGKK
>Mature_208_residues
TDLSQQFDRLIAQTDLTVSELQKEQLLGYVALLHKWNKAYNLTSVRNPSEMVIKHILDSIVVSPNLSGERFIDVGTGPGL
PGIPLAIMNPEKSFTLLDSLGKRIRFIKQVIHELKITNVTPIQSRVEDFQPEEKFDAVLSRAFASMTDMVNWCHHLPKED
GVFLALKGIYSEEEANDLPEWCSVRDVVTLIVPELEGERHLVTLTGKK

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=199, Percent_Identity=63.3165829145729, Blast_Score=268, Evalue=1e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: =2.1.1.170 [H]

Molecular weight: Translated: 23594; Mature: 23462

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLSQQFDRLIAQTDLTVSELQKEQLLGYVALLHKWNKAYNLTSVRNPSEMVIKHILDS
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
IVVSPNLSGERFIDVGTGPGLPGIPLAIMNPEKSFTLLDSLGKRIRFIKQVIHELKITNV
HHCCCCCCCCEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
TPIQSRVEDFQPEEKFDAVLSRAFASMTDMVNWCHHLPKEDGVFLALKGIYSEEEANDLP
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHCCCH
EWCSVRDVVTLIVPELEGERHLVTLTGKK
HHHHHHHHHHHHHCCCCCCEEEEEEECCC
>Mature Secondary Structure 
TDLSQQFDRLIAQTDLTVSELQKEQLLGYVALLHKWNKAYNLTSVRNPSEMVIKHILDS
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
IVVSPNLSGERFIDVGTGPGLPGIPLAIMNPEKSFTLLDSLGKRIRFIKQVIHELKITNV
HHCCCCCCCCEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
TPIQSRVEDFQPEEKFDAVLSRAFASMTDMVNWCHHLPKEDGVFLALKGIYSEEEANDLP
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHCCCH
EWCSVRDVVTLIVPELEGERHLVTLTGKK
HHHHHHHHHHHHHCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA