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The map label for this gene is hemB [H]

Identifier: 209696398

GI number: 209696398

Start: 3254843

End: 3255859

Strand: Direct

Name: hemB [H]

Synonym: VSAL_I3004

Alternate gene names: 209696398

Gene position: 3254843-3255859 (Clockwise)

Preceding gene: 209696396

Following gene: 209696399

Centisome position: 97.89

GC content: 45.43

Gene sequence:

>1017_bases
GTGTCTGTATCCATTCAAGGTACGTTTCCAAACCGTCGTATGCGTCGTATGCGTAAGCATGAATTTAGCCGTCGTTTAAT
GGCTGAGAACCAACTAACCGTTAATGATTTAATCTATCCAATGTTTGTGCTGATGGGTAAAGATCGTCGTGAAAGCGTAG
ATTCTATGCCTGGCGTTGATCGTTTATCGATTGATTTACTGCTTGAAGAAGCCCAGCGTTTATATGAGTTAGGGATCCCT
GCCATTGCATTGTTCCCGGTTGTGTCTCAGGACGTAAAAAGTTTATGTGCAGCAGAAGCTCATAACTCTGAAGGTCTTGT
TCAACGTGCCGTTAAAGCATTGAAAGAGCACATTCCTGAGCTAGGCGTGATCACTGATGTGGCATTAGACCCATTCACCA
CTCATGGGCAAGACGGGATCATCGATGAAGATGGTTACGTAATGAATGACATCACAACGGAAGTCTTGATCAAGCAAGCA
CTTTCTCACGCTGAAGCGGGTGCGGATGTTGTTGCACCATCAGATATGATGGATGGTCGTATTGGTGAAATCCGTAAAGC
GCTAGAAAAAGCGGGCCATGTTCATACACAAATCATGGCGTACTCTGCAAAATATGCCTCTTGTTATTACGGGCCGTTCC
GTGATGCCGTTGGTTCTTCGTCGAATCTTAAAGGTGGCAACAAGAAGAACTACCAAATGGATCCAGCCAACAGCGATGAA
GCGATTCATGAAGTAGCAATGGACATCAATGAAGGCGCTGACATGGTCATGGTGAAACCGGGTATGCCATATCTGGACAT
CGTTCGTCGTGTTAAGACTGAGCTTCAAGTACCTACCTTTGCTTATCAAGTATCAGGTGAATATGCGATGCACAAAGCCG
CTATTATGAATGGTTGGTTGAGTGAGCGTGATACGGTGATGGAGTCGTTATTGTGCTTCAAACGTGCCGGCGCTGATGGC
ATCCTAACTTACTTTGCAAAAGACGTAGCAGAGTGGTTGGCGGAAGAGAAGAAATAA

Upstream 100 bases:

>100_bases
AATAAGGAAGAGAAATATGATGAACATTTATGGGCGATAGTTGGATTCAAAAGACGATGGGTATATATTGAATGCATCAT
AAATACAAAAGGAGCTCAGT

Downstream 100 bases:

>100_bases
GATCAGGGTTCAGATCGCTTCGCTTAAAGGTTTCAGGGGTGGGTGTGGTGAGCTTTGGTGTTAACTAGAGACTATACGCT
GCGCTAACTAGAGGCTATTC

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MSVSIQGTFPNRRMRRMRKHEFSRRLMAENQLTVNDLIYPMFVLMGKDRRESVDSMPGVDRLSIDLLLEEAQRLYELGIP
AIALFPVVSQDVKSLCAAEAHNSEGLVQRAVKALKEHIPELGVITDVALDPFTTHGQDGIIDEDGYVMNDITTEVLIKQA
LSHAEAGADVVAPSDMMDGRIGEIRKALEKAGHVHTQIMAYSAKYASCYYGPFRDAVGSSSNLKGGNKKNYQMDPANSDE
AIHEVAMDINEGADMVMVKPGMPYLDIVRRVKTELQVPTFAYQVSGEYAMHKAAIMNGWLSERDTVMESLLCFKRAGADG
ILTYFAKDVAEWLAEEKK

Sequences:

>Translated_338_residues
MSVSIQGTFPNRRMRRMRKHEFSRRLMAENQLTVNDLIYPMFVLMGKDRRESVDSMPGVDRLSIDLLLEEAQRLYELGIP
AIALFPVVSQDVKSLCAAEAHNSEGLVQRAVKALKEHIPELGVITDVALDPFTTHGQDGIIDEDGYVMNDITTEVLIKQA
LSHAEAGADVVAPSDMMDGRIGEIRKALEKAGHVHTQIMAYSAKYASCYYGPFRDAVGSSSNLKGGNKKNYQMDPANSDE
AIHEVAMDINEGADMVMVKPGMPYLDIVRRVKTELQVPTFAYQVSGEYAMHKAAIMNGWLSERDTVMESLLCFKRAGADG
ILTYFAKDVAEWLAEEKK
>Mature_337_residues
SVSIQGTFPNRRMRRMRKHEFSRRLMAENQLTVNDLIYPMFVLMGKDRRESVDSMPGVDRLSIDLLLEEAQRLYELGIPA
IALFPVVSQDVKSLCAAEAHNSEGLVQRAVKALKEHIPELGVITDVALDPFTTHGQDGIIDEDGYVMNDITTEVLIKQAL
SHAEAGADVVAPSDMMDGRIGEIRKALEKAGHVHTQIMAYSAKYASCYYGPFRDAVGSSSNLKGGNKKNYQMDPANSDEA
IHEVAMDINEGADMVMVKPGMPYLDIVRRVKTELQVPTFAYQVSGEYAMHKAAIMNGWLSERDTVMESLLCFKRAGADGI
LTYFAKDVAEWLAEEKK

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=311, Percent_Identity=41.4790996784566, Blast_Score=232, Evalue=4e-61,
Organism=Escherichia coli, GI87081728, Length=320, Percent_Identity=45, Blast_Score=246, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6321398, Length=322, Percent_Identity=37.5776397515528, Blast_Score=210, Evalue=3e-55,
Organism=Drosophila melanogaster, GI21358291, Length=328, Percent_Identity=36.5853658536585, Blast_Score=217, Evalue=8e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 37596; Mature: 37464

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSIQGTFPNRRMRRMRKHEFSRRLMAENQLTVNDLIYPMFVLMGKDRRESVDSMPGVD
CEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCC
RLSIDLLLEEAQRLYELGIPAIALFPVVSQDVKSLCAAEAHNSEGLVQRAVKALKEHIPE
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
LGVITDVALDPFTTHGQDGIIDEDGYVMNDITTEVLIKQALSHAEAGADVVAPSDMMDGR
CCCHHHHCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCC
IGEIRKALEKAGHVHTQIMAYSAKYASCYYGPFRDAVGSSSNLKGGNKKNYQMDPANSDE
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCHH
AIHEVAMDINEGADMVMVKPGMPYLDIVRRVKTELQVPTFAYQVSGEYAMHKAAIMNGWL
HHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHH
SERDTVMESLLCFKRAGADGILTYFAKDVAEWLAEEKK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVSIQGTFPNRRMRRMRKHEFSRRLMAENQLTVNDLIYPMFVLMGKDRRESVDSMPGVD
EEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCC
RLSIDLLLEEAQRLYELGIPAIALFPVVSQDVKSLCAAEAHNSEGLVQRAVKALKEHIPE
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
LGVITDVALDPFTTHGQDGIIDEDGYVMNDITTEVLIKQALSHAEAGADVVAPSDMMDGR
CCCHHHHCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCC
IGEIRKALEKAGHVHTQIMAYSAKYASCYYGPFRDAVGSSSNLKGGNKKNYQMDPANSDE
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCHH
AIHEVAMDINEGADMVMVKPGMPYLDIVRRVKTELQVPTFAYQVSGEYAMHKAAIMNGWL
HHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHH
SERDTVMESLLCFKRAGADGILTYFAKDVAEWLAEEKK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9529530; 10984043; 10356331; 12079382 [H]