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The map label for this gene is glnA [H]

Identifier: 209696337

GI number: 209696337

Start: 3186733

End: 3188142

Strand: Direct

Name: glnA [H]

Synonym: VSAL_I2940

Alternate gene names: 209696337

Gene position: 3186733-3188142 (Clockwise)

Preceding gene: 209696330

Following gene: 209696338

Centisome position: 95.84

GC content: 43.4

Gene sequence:

>1410_bases
ATGTCAGTAGAAAACGTATTCGCTCTAATTCAAGAAAACGAAGTAAAATTTATTGATTTACGCTTTACCGATACAAAAGG
TAAAGAGCAACATATCTCAATTCCTTCTCATCAAGTTGATGCAGACTTCTTCGAAGACGGCAAAATGTTTGATGGTTCTT
CAGTTGCTGGCTGGAAAGGCATTAACGAATCTGACATGGTTATGATGCCAGATGCAGCAAGTGCGGTACTTGACCCATTC
ACTGCTGATGCAACCTTAAATATTCGTTGTGATATCCTTGAACCAACAACAATGCAAGGTTACGATCGTGACCCTCGCTC
AATTGCAAAACGCTCTGAAGACTACTTACGCTCGACAGGTCTTGCTGACACAGTTTTAGTCGGTCCAGAACCAGAATTCT
TCCTATTTGATGACGTTCGCTTTAGCACTGATATGTCAGGTTCTTTCTTTAAGATTGATGATATCGAAGCCGCGTGGAAC
TCAGGTACTGAATACGAAGGCGGCAACAAAGGCCACCGTCCTGGCGTTAAAGGGGGTTACTTCCCAGTCGCTCCTGTTGA
TTCATCTCAAGATATCCGTTCTGCTATGTGTTTAATCATGGAAGAAATGGGTCTTGTTGTTGAAGCACATCACCACGAAG
TAGCAACGGCGGGTCAAAATGAGATCGCAACTCGCTTCAATACGCTAACAAACAAGGCAGATGAAACTCAAATCTATAAG
TATGTTGTTCATAACGTAGCTCACGCTTACGGTAAAACAGCGACTTTCATGCCAAAACCACTGGTTGGTGACAACGGTTC
GGGCATGCACGTACATATGTCATTGAACAAAGATGGACAAAACTTATTTGCTGGCGATAAGTACGGCGGCCTATCAGAAA
TGGCTATCTTCTTTATTGGCGGTATCATTAAACACGCTCGTGCTATCAACGCATTTGCTAACCCAACAACTAACTCATAC
AAGCGTCTTGTCCCTGGTTTTGAAGCTCCGGTAATGCTTGCATATTCTGCTCGTAACCGTTCAGCGTCTATCCGTATCCC
AGTGGTACCAAGCCCGAAAGCACGTCGTATCGAAGTTCGTTTTGGTGACCCTGCAGCAAACCCATACTTAAGCTACTCAG
CAATGCTAATGGCTGGTCTTGACGGAATTAAGAACAAGATCCACCCAGGTGAAGCAATGGATAAAGATCTGTATGACCTT
CCTGCGGAAGAAGCAGCTGAAATTCCAAAAGTAGCTGAATCTCTAGATATCGCACTACAAGCTCTGGATTCTGATCGTGA
ATTCTTAACGGCGGGTGGCGTATTCTCTAGTGATTTCATTGATTCTTACATCACTCTAAAATCTCAAGACGTTGCCGCTA
TCAACACTGCTGTTCACCCTCTTGAATTTGAAATGTATTACTCAGTTTAA

Upstream 100 bases:

>100_bases
TAATATTAAAGGATTATTACTTTTGGCACAGTTTTGGCTATATAGTTAATAACAGCCTAACTGGCCAATATTTATATTAT
AACACCGGAGGTTATCCTAC

Downstream 100 bases:

>100_bases
TTCTGACGTAACAAATTGAAATCAATCTGATGTTTTTAGGCTCGCTTCACAAGCGAGCCTTTTTTATATTGGCAAAACCT
CATTTACACGCAACACTCCT

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSVENVFALIQENEVKFIDLRFTDTKGKEQHISIPSHQVDADFFEDGKMFDGSSVAGWKGINESDMVMMPDAASAVLDPF
TADATLNIRCDILEPTTMQGYDRDPRSIAKRSEDYLRSTGLADTVLVGPEPEFFLFDDVRFSTDMSGSFFKIDDIEAAWN
SGTEYEGGNKGHRPGVKGGYFPVAPVDSSQDIRSAMCLIMEEMGLVVEAHHHEVATAGQNEIATRFNTLTNKADETQIYK
YVVHNVAHAYGKTATFMPKPLVGDNGSGMHVHMSLNKDGQNLFAGDKYGGLSEMAIFFIGGIIKHARAINAFANPTTNSY
KRLVPGFEAPVMLAYSARNRSASIRIPVVPSPKARRIEVRFGDPAANPYLSYSAMLMAGLDGIKNKIHPGEAMDKDLYDL
PAEEAAEIPKVAESLDIALQALDSDREFLTAGGVFSSDFIDSYITLKSQDVAAINTAVHPLEFEMYYSV

Sequences:

>Translated_469_residues
MSVENVFALIQENEVKFIDLRFTDTKGKEQHISIPSHQVDADFFEDGKMFDGSSVAGWKGINESDMVMMPDAASAVLDPF
TADATLNIRCDILEPTTMQGYDRDPRSIAKRSEDYLRSTGLADTVLVGPEPEFFLFDDVRFSTDMSGSFFKIDDIEAAWN
SGTEYEGGNKGHRPGVKGGYFPVAPVDSSQDIRSAMCLIMEEMGLVVEAHHHEVATAGQNEIATRFNTLTNKADETQIYK
YVVHNVAHAYGKTATFMPKPLVGDNGSGMHVHMSLNKDGQNLFAGDKYGGLSEMAIFFIGGIIKHARAINAFANPTTNSY
KRLVPGFEAPVMLAYSARNRSASIRIPVVPSPKARRIEVRFGDPAANPYLSYSAMLMAGLDGIKNKIHPGEAMDKDLYDL
PAEEAAEIPKVAESLDIALQALDSDREFLTAGGVFSSDFIDSYITLKSQDVAAINTAVHPLEFEMYYSV
>Mature_468_residues
SVENVFALIQENEVKFIDLRFTDTKGKEQHISIPSHQVDADFFEDGKMFDGSSVAGWKGINESDMVMMPDAASAVLDPFT
ADATLNIRCDILEPTTMQGYDRDPRSIAKRSEDYLRSTGLADTVLVGPEPEFFLFDDVRFSTDMSGSFFKIDDIEAAWNS
GTEYEGGNKGHRPGVKGGYFPVAPVDSSQDIRSAMCLIMEEMGLVVEAHHHEVATAGQNEIATRFNTLTNKADETQIYKY
VVHNVAHAYGKTATFMPKPLVGDNGSGMHVHMSLNKDGQNLFAGDKYGGLSEMAIFFIGGIIKHARAINAFANPTTNSYK
RLVPGFEAPVMLAYSARNRSASIRIPVVPSPKARRIEVRFGDPAANPYLSYSAMLMAGLDGIKNKIHPGEAMDKDLYDLP
AEEAAEIPKVAESLDIALQALDSDREFLTAGGVFSSDFIDSYITLKSQDVAAINTAVHPLEFEMYYSV

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=469, Percent_Identity=77.8251599147121, Blast_Score=790, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=297, Percent_Identity=29.2929292929293, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 51482; Mature: 51350

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVENVFALIQENEVKFIDLRFTDTKGKEQHISIPSHQVDADFFEDGKMFDGSSVAGWKG
CCHHHEEHEEECCCEEEEEEEEECCCCCCCEECCCCCCCCCHHHCCCCEECCCCCCCCCC
INESDMVMMPDAASAVLDPFTADATLNIRCDILEPTTMQGYDRDPRSIAKRSEDYLRSTG
CCCCCEEECCCHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCC
LADTVLVGPEPEFFLFDDVRFSTDMSGSFFKIDDIEAAWNSGTEYEGGNKGHRPGVKGGY
CCCEEEECCCCCEEEEECEEECCCCCCCEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCC
FPVAPVDSSQDIRSAMCLIMEEMGLVVEAHHHEVATAGQNEIATRFNTLTNKADETQIYK
EECCCCCCCHHHHHHHHHHHHHCCCEEEECCCHHHHCCCHHHHHHHHHHHCCCCHHHHHH
YVVHNVAHAYGKTATFMPKPLVGDNGSGMHVHMSLNKDGQNLFAGDKYGGLSEMAIFFIG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHHHH
GIIKHARAINAFANPTTNSYKRLVPGFEAPVMLAYSARNRSASIRIPVVPSPKARRIEVR
HHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCCCEEEEEE
FGDPAANPYLSYSAMLMAGLDGIKNKIHPGEAMDKDLYDLPAEEAAEIPKVAESLDIALQ
ECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHCCHHHHHHHHHHHHHHHHHHH
ALDSDREFLTAGGVFSSDFIDSYITLKSQDVAAINTAVHPLEFEMYYSV
HHCCCCCEEEECCCCCHHHHHHEEEECCCCCEEHHHCCCCEEEEEEECC
>Mature Secondary Structure 
SVENVFALIQENEVKFIDLRFTDTKGKEQHISIPSHQVDADFFEDGKMFDGSSVAGWKG
CHHHEEHEEECCCEEEEEEEEECCCCCCCEECCCCCCCCCHHHCCCCEECCCCCCCCCC
INESDMVMMPDAASAVLDPFTADATLNIRCDILEPTTMQGYDRDPRSIAKRSEDYLRSTG
CCCCCEEECCCHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCC
LADTVLVGPEPEFFLFDDVRFSTDMSGSFFKIDDIEAAWNSGTEYEGGNKGHRPGVKGGY
CCCEEEECCCCCEEEEECEEECCCCCCCEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCC
FPVAPVDSSQDIRSAMCLIMEEMGLVVEAHHHEVATAGQNEIATRFNTLTNKADETQIYK
EECCCCCCCHHHHHHHHHHHHHCCCEEEECCCHHHHCCCHHHHHHHHHHHCCCCHHHHHH
YVVHNVAHAYGKTATFMPKPLVGDNGSGMHVHMSLNKDGQNLFAGDKYGGLSEMAIFFIG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHHHH
GIIKHARAINAFANPTTNSYKRLVPGFEAPVMLAYSARNRSASIRIPVVPSPKARRIEVR
HHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCCCEEEEEE
FGDPAANPYLSYSAMLMAGLDGIKNKIHPGEAMDKDLYDLPAEEAAEIPKVAESLDIALQ
ECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHCCHHHHHHHHHHHHHHHHHHH
ALDSDREFLTAGGVFSSDFIDSYITLKSQDVAAINTAVHPLEFEMYYSV
HHCCCCCEEEECCCCCHHHHHHEEEECCCCCEEHHHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]