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The map label for this gene is purD [H]

Identifier: 209696247

GI number: 209696247

Start: 3081756

End: 3083048

Strand: Direct

Name: purD [H]

Synonym: VSAL_I2843

Alternate gene names: 209696247

Gene position: 3081756-3083048 (Clockwise)

Preceding gene: 209696246

Following gene: 209696248

Centisome position: 92.68

GC content: 45.94

Gene sequence:

>1293_bases
ATGCGAGTTTTAATCATCGGTGCCGGCGGTCGTGAGCACGCGCTTGGCTGGAAAGCAGCACAAAACCCAAACGTTGAAAC
GGTATTTATCGCTCCAGGTAATGCAGGTACAGCGCTTGAACCAAAATTAGAAAACGTAGCGATTAATGTTGAAGACATCG
CTAGCCTTGTTGCATTTGCTAAAGAAAAAGCCATTGAACTGACTATCGTTGGTCCAGAAGGCCCATTAGTGATTGGTGTG
GTTGATGCATTCCGTGAAGCAGGATTACCTATCTTTGGTCCAACGAAAGCGGCAGCACAACTAGAAGGATCTAAAGCGTT
TACTAAAGACTTTTTAGCTCGTCATGATATTCCGACAGGCTACTACTCTAACTTCACAGAGATAGAGCCTGCATTAGCGT
ATGTACGTGAGCAAGGCGCACCTATCGTTGTTAAAGCCGATGGTCTTGCTGCGGGTAAAGGCGTTATCGTTGCGATGACT
CTGAAAGAAGCTGAAGATGCTATCAAAGACATGCTTTCTGACAATGTTTTTGGTGAAGCCGGTAGCCGTGTCGTTATAGA
AGAGTTCTTAGAAGGTGAAGAAGCAAGCTTCATCGTGATGGTTGATGGCGCTAGCGTCTTACCAATGGCAACAAGCCAAG
ATCATAAACGTGTTGGTGATAAAGATACAGGCCCTAACACGGGTGGCATGGGTGCATATTCTCCAGCGCCTGTTGTCACA
CCTGAAATCCATAACCGTATTCTAGAAGAAGTTATCTACCCTACCGTTCGCGGCATGGATGCAGAAGGCGCACCTTACAC
TGGCTTCCTATATGCAGGTTTGATGATTGATGCTGATGGTACACCAAAGGTAATCGAATATAACTGCCGCTTTGGTGATC
CAGAAACACAACCAATTATGATGCGCATGGAATCAGATCTTGTAGAACTGTGTTTAATGGCTATCGATGAAAAACTGGAT
GAAGCAGAATCTAAATGGGATCCTCGTGCTGCTATTGGTGTTGTTCTAGCTGCCGGCGGTTACCCTGCCGATTATGCAAA
AGGTGATGTTATTTCTCTTCCTGCAACAGAACTTGAAGGTCAGAAAATATTCCACGCAGGAACAACCAACAACGCCGCTG
GTGATGTGACAACAAATGGTGGACGTGTACTTTGTGCGACCGCACTCGGTAATACCGTCTCAGAAGCTCAAGAGCGCGCG
TATGAGCTAACTAAACAAGTAAGTTGGAATGGAATGTTCCATCGCAATGACATCGGTTACCGCGCCATTGCTCGCGAATT
AGAAAGCAAGTAA

Upstream 100 bases:

>100_bases
GGGCTCAATTGTTGCCTAGTTACAGCACCAACTATTTTGAATAAAAAATTAATCATTTTGTTTTTTGAATATTTAGATAT
TTGAGAGTAAGGATTTAACA

Downstream 100 bases:

>100_bases
ACATTACTTTTCTACAAATAACAAAAAAGGCGCTGTGATAGCTAATGCCGATCATTTAAGGTAAATATGATCGGTTGACC
GATCCTTTTTAGATGTCAAA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MRVLIIGAGGREHALGWKAAQNPNVETVFIAPGNAGTALEPKLENVAINVEDIASLVAFAKEKAIELTIVGPEGPLVIGV
VDAFREAGLPIFGPTKAAAQLEGSKAFTKDFLARHDIPTGYYSNFTEIEPALAYVREQGAPIVVKADGLAAGKGVIVAMT
LKEAEDAIKDMLSDNVFGEAGSRVVIEEFLEGEEASFIVMVDGASVLPMATSQDHKRVGDKDTGPNTGGMGAYSPAPVVT
PEIHNRILEEVIYPTVRGMDAEGAPYTGFLYAGLMIDADGTPKVIEYNCRFGDPETQPIMMRMESDLVELCLMAIDEKLD
EAESKWDPRAAIGVVLAAGGYPADYAKGDVISLPATELEGQKIFHAGTTNNAAGDVTTNGGRVLCATALGNTVSEAQERA
YELTKQVSWNGMFHRNDIGYRAIARELESK

Sequences:

>Translated_430_residues
MRVLIIGAGGREHALGWKAAQNPNVETVFIAPGNAGTALEPKLENVAINVEDIASLVAFAKEKAIELTIVGPEGPLVIGV
VDAFREAGLPIFGPTKAAAQLEGSKAFTKDFLARHDIPTGYYSNFTEIEPALAYVREQGAPIVVKADGLAAGKGVIVAMT
LKEAEDAIKDMLSDNVFGEAGSRVVIEEFLEGEEASFIVMVDGASVLPMATSQDHKRVGDKDTGPNTGGMGAYSPAPVVT
PEIHNRILEEVIYPTVRGMDAEGAPYTGFLYAGLMIDADGTPKVIEYNCRFGDPETQPIMMRMESDLVELCLMAIDEKLD
EAESKWDPRAAIGVVLAAGGYPADYAKGDVISLPATELEGQKIFHAGTTNNAAGDVTTNGGRVLCATALGNTVSEAQERA
YELTKQVSWNGMFHRNDIGYRAIARELESK
>Mature_430_residues
MRVLIIGAGGREHALGWKAAQNPNVETVFIAPGNAGTALEPKLENVAINVEDIASLVAFAKEKAIELTIVGPEGPLVIGV
VDAFREAGLPIFGPTKAAAQLEGSKAFTKDFLARHDIPTGYYSNFTEIEPALAYVREQGAPIVVKADGLAAGKGVIVAMT
LKEAEDAIKDMLSDNVFGEAGSRVVIEEFLEGEEASFIVMVDGASVLPMATSQDHKRVGDKDTGPNTGGMGAYSPAPVVT
PEIHNRILEEVIYPTVRGMDAEGAPYTGFLYAGLMIDADGTPKVIEYNCRFGDPETQPIMMRMESDLVELCLMAIDEKLD
EAESKWDPRAAIGVVLAAGGYPADYAKGDVISLPATELEGQKIFHAGTTNNAAGDVTTNGGRVLCATALGNTVSEAQERA
YELTKQVSWNGMFHRNDIGYRAIARELESK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=426, Percent_Identity=48.5915492957746, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI209869995, Length=426, Percent_Identity=48.5915492957746, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI209869993, Length=426, Percent_Identity=48.5915492957746, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI28416899, Length=426, Percent_Identity=48.5915492957746, Blast_Score=399, Evalue=1e-111,
Organism=Escherichia coli, GI1790438, Length=427, Percent_Identity=73.3021077283372, Blast_Score=644, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17567511, Length=424, Percent_Identity=41.0377358490566, Blast_Score=303, Evalue=8e-83,
Organism=Saccharomyces cerevisiae, GI6321203, Length=446, Percent_Identity=43.7219730941704, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=45.5813953488372, Blast_Score=352, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24582400, Length=430, Percent_Identity=45.5813953488372, Blast_Score=351, Evalue=4e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45790; Mature: 45790

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLIIGAGGREHALGWKAAQNPNVETVFIAPGNAGTALEPKLENVAINVEDIASLVAFA
CEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEECHHHHHHHHHHH
KEKAIELTIVGPEGPLVIGVVDAFREAGLPIFGPTKAAAQLEGSKAFTKDFLARHDIPTG
HCCEEEEEEECCCCCEEEEEHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCC
YYSNFTEIEPALAYVREQGAPIVVKADGLAAGKGVIVAMTLKEAEDAIKDMLSDNVFGEA
CCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCC
GSRVVIEEFLEGEEASFIVMVDGASVLPMATSQDHKRVGDKDTGPNTGGMGAYSPAPVVT
CCEEHHHHHHCCCCCEEEEEECCCCEECCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCC
PEIHNRILEEVIYPTVRGMDAEGAPYTGFLYAGLMIDADGTPKVIEYNCRFGDPETQPIM
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECCCCCCEEEEEECCCCCCCCCEE
MRMESDLVELCLMAIDEKLDEAESKWDPRAAIGVVLAAGGYPADYAKGDVISLPATELEG
EHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCEEECCCCCCCC
QKIFHAGTTNNAAGDVTTNGGRVLCATALGNTVSEAQERAYELTKQVSWNGMFHRNDIGY
CEEEEECCCCCCCCCEECCCCEEEEEEHHCCHHHHHHHHHHHHHHHHCCCCCEECCCCCH
RAIARELESK
HHHHHHHHCC
>Mature Secondary Structure
MRVLIIGAGGREHALGWKAAQNPNVETVFIAPGNAGTALEPKLENVAINVEDIASLVAFA
CEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEECHHHHHHHHHHH
KEKAIELTIVGPEGPLVIGVVDAFREAGLPIFGPTKAAAQLEGSKAFTKDFLARHDIPTG
HCCEEEEEEECCCCCEEEEEHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCC
YYSNFTEIEPALAYVREQGAPIVVKADGLAAGKGVIVAMTLKEAEDAIKDMLSDNVFGEA
CCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCC
GSRVVIEEFLEGEEASFIVMVDGASVLPMATSQDHKRVGDKDTGPNTGGMGAYSPAPVVT
CCEEHHHHHHCCCCCEEEEEECCCCEECCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCC
PEIHNRILEEVIYPTVRGMDAEGAPYTGFLYAGLMIDADGTPKVIEYNCRFGDPETQPIM
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECCCCCCEEEEEECCCCCCCCCEE
MRMESDLVELCLMAIDEKLDEAESKWDPRAAIGVVLAAGGYPADYAKGDVISLPATELEG
EHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCEEECCCCCCCC
QKIFHAGTTNNAAGDVTTNGGRVLCATALGNTVSEAQERAYELTKQVSWNGMFHRNDIGY
CEEEEECCCCCCCCCEECCCCEEEEEEHHCCHHHHHHHHHHHHHHHHCCCCCEECCCCCH
RAIARELESK
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]