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The map label for this gene is nagB

Identifier: 209696216

GI number: 209696216

Start: 3044823

End: 3045623

Strand: Direct

Name: nagB

Synonym: VSAL_I2812

Alternate gene names: 209696216

Gene position: 3044823-3045623 (Clockwise)

Preceding gene: 209696215

Following gene: 209696217

Centisome position: 91.57

GC content: 39.08

Gene sequence:

>801_bases
ATGAGACTTATTCCATTAAACCAAGCAGAACAAGTAGGTGCATGGTCAGCACAACACATTGTTAACCGCATCAATGAGTT
TAACCCAACAGCAGATCGTCCATTTGTTCTTGGTCTTCCTACTGGTGGAACTCCTCTTAATACTTACAAAAAGCTAATTG
AACTGTACAAAGCAGACAAAGTAAGCTTTAAGAACGTAGTAACATTCAACATGGATGAGTACATCGGTCTTGATACAAAC
CACCCAGAATCTTACCGTACTTTCATGTTTGAAAACTTTTTCAACCATGTTGATATCCAAGAAGAAAACATTAACCTTCT
AAACGGTAACACTGACAACCACGAAGAAGAATGCCAGCGTTACGAAGACAAAATTAAGTCTTACGGTCGCATTAACTTGT
TCATGGGTGGTGTTGGTAACGACGGTCATATCGCATTTAACGAACCAGCTTCATCTCTAGCATCTCGTACTCGTATCAAA
ACACTAACTGAAGATACTCGTATTGCTAACTCTCGCTTCTTCGACGGCGATATTGCTCAAGTACCTAAGTACGCATTAAC
TATCGGTGTTGGTACTCTGCTTGATTCTGCTGAAATCATGATCCTAATTACTGGCCACAACAAAGCACTTGCTCTACAAG
CTGCTGTTGAAGGTTCTGTAAACCACATGTGGACAGTATCAGCACTTCAAATTCACCCTAAATCAGTGATCGTTTGTGAT
GAAGCATCACAACAAGAACTGAAAGTGAAAACAGTTAAATACTTTAAAGAACTAGAAGCTGAAAATATTAAACACGTTTA
A

Upstream 100 bases:

>100_bases
CCTTTATTGACCAAAATCATTAGCATTACGAAGGTACTTATTTTACAATGCGAATTAACGCAATTAAGCGAACAATTACA
TTAATTTTTTGAGGTATATG

Downstream 100 bases:

>100_bases
TCTTCTGCTTTATACATCGCAAAAAGGAGAACTTATGTTCTCCTTTTTTTATGTCTATTTTTGGGATTATCATTTCAACA
CCATTCCATTAATCCACATT

Product: glucosamine-6-phosphate deaminase

Products: NA

Alternate protein names: GlcN6P deaminase; GNPDA; Glucosamine-6-phosphate isomerase

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MRLIPLNQAEQVGAWSAQHIVNRINEFNPTADRPFVLGLPTGGTPLNTYKKLIELYKADKVSFKNVVTFNMDEYIGLDTN
HPESYRTFMFENFFNHVDIQEENINLLNGNTDNHEEECQRYEDKIKSYGRINLFMGGVGNDGHIAFNEPASSLASRTRIK
TLTEDTRIANSRFFDGDIAQVPKYALTIGVGTLLDSAEIMILITGHNKALALQAAVEGSVNHMWTVSALQIHPKSVIVCD
EASQQELKVKTVKYFKELEAENIKHV

Sequences:

>Translated_266_residues
MRLIPLNQAEQVGAWSAQHIVNRINEFNPTADRPFVLGLPTGGTPLNTYKKLIELYKADKVSFKNVVTFNMDEYIGLDTN
HPESYRTFMFENFFNHVDIQEENINLLNGNTDNHEEECQRYEDKIKSYGRINLFMGGVGNDGHIAFNEPASSLASRTRIK
TLTEDTRIANSRFFDGDIAQVPKYALTIGVGTLLDSAEIMILITGHNKALALQAAVEGSVNHMWTVSALQIHPKSVIVCD
EASQQELKVKTVKYFKELEAENIKHV
>Mature_266_residues
MRLIPLNQAEQVGAWSAQHIVNRINEFNPTADRPFVLGLPTGGTPLNTYKKLIELYKADKVSFKNVVTFNMDEYIGLDTN
HPESYRTFMFENFFNHVDIQEENINLLNGNTDNHEEECQRYEDKIKSYGRINLFMGGVGNDGHIAFNEPASSLASRTRIK
TLTEDTRIANSRFFDGDIAQVPKYALTIGVGTLLDSAEIMILITGHNKALALQAAVEGSVNHMWTVSALQIHPKSVIVCD
EASQQELKVKTVKYFKELEAENIKHV

Specific function: Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily

Homologues:

Organism=Homo sapiens, GI13027378, Length=258, Percent_Identity=60.4651162790698, Blast_Score=335, Evalue=3e-92,
Organism=Homo sapiens, GI19923881, Length=258, Percent_Identity=58.5271317829457, Blast_Score=328, Evalue=3e-90,
Organism=Escherichia coli, GI1786893, Length=264, Percent_Identity=75.3787878787879, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI1789530, Length=231, Percent_Identity=26.4069264069264, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI48994958, Length=241, Percent_Identity=26.5560165975104, Blast_Score=76, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17554876, Length=258, Percent_Identity=58.5271317829457, Blast_Score=312, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24581960, Length=256, Percent_Identity=58.984375, Blast_Score=334, Evalue=3e-92,
Organism=Drosophila melanogaster, GI19920764, Length=256, Percent_Identity=58.984375, Blast_Score=334, Evalue=3e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAGB_ALISL (B6EN78)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002264146.1
- ProteinModelPortal:   B6EN78
- GeneID:   6988426
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2812
- HOGENOM:   HBG725991
- OMA:   HLSITMG
- ProtClustDB:   PRK00443
- HAMAP:   MF_01241
- InterPro:   IPR006148
- InterPro:   IPR004547
- InterPro:   IPR018321
- PANTHER:   PTHR11280
- TIGRFAMs:   TIGR00502

Pfam domain/function: PF01182 Glucosamine_iso

EC number: =3.5.99.6

Molecular weight: Translated: 29974; Mature: 29974

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: ACT_SITE 72-72 ACT_SITE 141-141 ACT_SITE 143-143 ACT_SITE 148-148

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLIPLNQAEQVGAWSAQHIVNRINEFNPTADRPFVLGLPTGGTPLNTYKKLIELYKADK
CEECCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCC
VSFKNVVTFNMDEYIGLDTNHPESYRTFMFENFFNHVDIQEENINLLNGNTDNHEEECQR
CCCCEEEEECCHHHCCCCCCCCHHHHHHHHHHHHCCCCEECCCEEEECCCCCCHHHHHHH
YEDKIKSYGRINLFMGGVGNDGHIAFNEPASSLASRTRIKTLTEDTRIANSRFFDGDIAQ
HHHHHHCCCEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
VPKYALTIGVGTLLDSAEIMILITGHNKALALQAAVEGSVNHMWTVSALQIHPKSVIVCD
CCCEEEEEEHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCEEEEEEEEEECCCEEEEEC
EASQQELKVKTVKYFKELEAENIKHV
CCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRLIPLNQAEQVGAWSAQHIVNRINEFNPTADRPFVLGLPTGGTPLNTYKKLIELYKADK
CEECCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCC
VSFKNVVTFNMDEYIGLDTNHPESYRTFMFENFFNHVDIQEENINLLNGNTDNHEEECQR
CCCCEEEEECCHHHCCCCCCCCHHHHHHHHHHHHCCCCEECCCEEEECCCCCCHHHHHHH
YEDKIKSYGRINLFMGGVGNDGHIAFNEPASSLASRTRIKTLTEDTRIANSRFFDGDIAQ
HHHHHHCCCEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
VPKYALTIGVGTLLDSAEIMILITGHNKALALQAAVEGSVNHMWTVSALQIHPKSVIVCD
CCCEEEEEEHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCEEEEEEEEEECCCEEEEEC
EASQQELKVKTVKYFKELEAENIKHV
CCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA