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The map label for this gene is pfkA

Identifier: 209696200

GI number: 209696200

Start: 3026252

End: 3027214

Strand: Reverse

Name: pfkA

Synonym: VSAL_I2794

Alternate gene names: 209696200

Gene position: 3027214-3026252 (Counterclockwise)

Preceding gene: 209696201

Following gene: 209696198

Centisome position: 91.04

GC content: 41.85

Gene sequence:

>963_bases
ATGGTCAAGAAGATTGGTGTTTTAACAAGTGGTGGCGATGCGCCGGGTATGAATGCTGCAGTTCGTGGTGTTGTTCGTAC
AGCCTTAACGCAAGGTTTAGAAGTATTCGGTATTCATGATGGCTACTTAGGTCTTGTTGAAGATCGTATCGAGAAATTAG
AGCGCCATAGCGTATCTGATATGATCAACCGCGGCGGTACATTTTTAGGTTCAGCACGTTTTCCTGAATTTAAAGAAGTG
GCTGTTCGTGAAAAAGCGATCGCAAATCTTAAGAAACACGATATTGATGCCTTAATTGTTATCGGTGGTGATGGTTCTTA
CATGGGCGCTAAGAAGTTAACTGAGATGGGTTACCCATGTATCGGTCTTCCTGGCACTATCGATAATGATATTGCGGGTA
CTGACTATACAATTGGTTACTTAACGGCATTAAACACCGTTATTGATGCGATTGACCGTTTACGTGATACATCGTCTTCT
CACCAACGTATTTCTATTGTAGAAGTTATGGGTCGTCACTGTGGTGATTTGACTTTAATGGCTGCAATTGCCGGTGGTTG
TGAATATGTAATTACACCTGAAACGGGGTTAAACAAAGAAGCATTAATTCAAAATATCCAAGATGGTATTGCGAAAGGTA
AGAAACACGCAATCATTGCGATTACTGAATTGATGACTGATGTAAACGCATTAGCAAAAGAAATCGAAGCAGAAACAGGT
CGTGAGACTCGTGCTACGGTTCTTGGTCACATTCAACGTGGCGGTCAGCCTGGTGCATTCGACCGTATTTTAGCGTCTCG
TATGGGTAATTACGGTGTTAAGTTGCTTGTTGAAGGTCATGGCGGTCGCTGTGTTGGTATCCAAAATGAACAACTTGTTC
ATCACGATATTATCGATGCGATTGAAAATATGCGTCGTCCTGAGAAGCTAGAACTTTATAAAGTAGCGGAAGAGTTATTC
TAA

Upstream 100 bases:

>100_bases
GAAAAAAAATTACAGATTATTAATGGCGAGTTGGTTAGGGAGTCGACTTGCTGTATTAAAAATTGAAATTGTCATTCCCA
ACCATATTTAGAGGGTAACC

Downstream 100 bases:

>100_bases
TTTAGAATACTGTTAAGTTATAAAAGAAAAACCGAAGTATAAGCTTCGGTTTTTTTATATCTGGAAAATAGGTCGATTTA
TATCGCTCTATTCTAAATAT

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MVKKIGVLTSGGDAPGMNAAVRGVVRTALTQGLEVFGIHDGYLGLVEDRIEKLERHSVSDMINRGGTFLGSARFPEFKEV
AVREKAIANLKKHDIDALIVIGGDGSYMGAKKLTEMGYPCIGLPGTIDNDIAGTDYTIGYLTALNTVIDAIDRLRDTSSS
HQRISIVEVMGRHCGDLTLMAAIAGGCEYVITPETGLNKEALIQNIQDGIAKGKKHAIIAITELMTDVNALAKEIEAETG
RETRATVLGHIQRGGQPGAFDRILASRMGNYGVKLLVEGHGGRCVGIQNEQLVHHDIIDAIENMRRPEKLELYKVAEELF

Sequences:

>Translated_320_residues
MVKKIGVLTSGGDAPGMNAAVRGVVRTALTQGLEVFGIHDGYLGLVEDRIEKLERHSVSDMINRGGTFLGSARFPEFKEV
AVREKAIANLKKHDIDALIVIGGDGSYMGAKKLTEMGYPCIGLPGTIDNDIAGTDYTIGYLTALNTVIDAIDRLRDTSSS
HQRISIVEVMGRHCGDLTLMAAIAGGCEYVITPETGLNKEALIQNIQDGIAKGKKHAIIAITELMTDVNALAKEIEAETG
RETRATVLGHIQRGGQPGAFDRILASRMGNYGVKLLVEGHGGRCVGIQNEQLVHHDIIDAIENMRRPEKLELYKVAEELF
>Mature_320_residues
MVKKIGVLTSGGDAPGMNAAVRGVVRTALTQGLEVFGIHDGYLGLVEDRIEKLERHSVSDMINRGGTFLGSARFPEFKEV
AVREKAIANLKKHDIDALIVIGGDGSYMGAKKLTEMGYPCIGLPGTIDNDIAGTDYTIGYLTALNTVIDAIDRLRDTSSS
HQRISIVEVMGRHCGDLTLMAAIAGGCEYVITPETGLNKEALIQNIQDGIAKGKKHAIIAITELMTDVNALAKEIEAETG
RETRATVLGHIQRGGQPGAFDRILASRMGNYGVKLLVEGHGGRCVGIQNEQLVHHDIIDAIENMRRPEKLELYKVAEELF

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family

Homologues:

Organism=Homo sapiens, GI266453619, Length=314, Percent_Identity=42.9936305732484, Blast_Score=214, Evalue=6e-56,
Organism=Homo sapiens, GI266453768, Length=314, Percent_Identity=42.9936305732484, Blast_Score=214, Evalue=6e-56,
Organism=Homo sapiens, GI266453748, Length=314, Percent_Identity=42.9936305732484, Blast_Score=214, Evalue=6e-56,
Organism=Homo sapiens, GI4505749, Length=314, Percent_Identity=42.9936305732484, Blast_Score=214, Evalue=6e-56,
Organism=Homo sapiens, GI11321601, Length=311, Percent_Identity=40.8360128617363, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI48762920, Length=311, Percent_Identity=42.1221864951768, Blast_Score=205, Evalue=4e-53,
Organism=Escherichia coli, GI1790350, Length=320, Percent_Identity=75.3125, Blast_Score=499, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI25147584, Length=311, Percent_Identity=38.2636655948553, Blast_Score=195, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17558788, Length=308, Percent_Identity=39.9350649350649, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI72003601, Length=290, Percent_Identity=30.3448275862069, Blast_Score=141, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6321679, Length=369, Percent_Identity=36.8563685636856, Blast_Score=202, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6323861, Length=341, Percent_Identity=34.3108504398827, Blast_Score=177, Evalue=2e-45,
Organism=Drosophila melanogaster, GI28573326, Length=312, Percent_Identity=40.7051282051282, Blast_Score=204, Evalue=7e-53,
Organism=Drosophila melanogaster, GI17647809, Length=312, Percent_Identity=40.0641025641026, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24652337, Length=311, Percent_Identity=40.1929260450161, Blast_Score=199, Evalue=2e-51,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): K6PF_ALISL (B6EMS4)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002264130.1
- ProteinModelPortal:   B6EMS4
- GeneID:   6987920
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2794
- HOGENOM:   HBG405044
- OMA:   QKENINA
- ProtClustDB:   PRK03202
- GO:   GO:0006096
- HAMAP:   MF_00339
- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023
- PIRSF:   PIRSF000532
- PRINTS:   PR00476
- TIGRFAMs:   TIGR02482

Pfam domain/function: PF00365 PFK; SSF53784 Ppfruckinase

EC number: =2.7.1.11

Molecular weight: Translated: 34559; Mature: 34559

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: ACT_SITE 128-128 BINDING 163-163 BINDING 244-244 BINDING 250-250 BINDING 253-253

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKIGVLTSGGDAPGMNAAVRGVVRTALTQGLEVFGIHDGYLGLVEDRIEKLERHSVSD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHH
MINRGGTFLGSARFPEFKEVAVREKAIANLKKHDIDALIVIGGDGSYMGAKKLTEMGYPC
HHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHCCCCC
IGLPGTIDNDIAGTDYTIGYLTALNTVIDAIDRLRDTSSSHQRISIVEVMGRHCGDLTLM
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHH
AAIAGGCEYVITPETGLNKEALIQNIQDGIAKGKKHAIIAITELMTDVNALAKEIEAETG
HHHHCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCC
RETRATVLGHIQRGGQPGAFDRILASRMGNYGVKLLVEGHGGRCVGIQNEQLVHHDIIDA
CHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHH
IENMRRPEKLELYKVAEELF
HHHCCCCHHHHHHHHHHHHC
>Mature Secondary Structure
MVKKIGVLTSGGDAPGMNAAVRGVVRTALTQGLEVFGIHDGYLGLVEDRIEKLERHSVSD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHH
MINRGGTFLGSARFPEFKEVAVREKAIANLKKHDIDALIVIGGDGSYMGAKKLTEMGYPC
HHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHCCCCC
IGLPGTIDNDIAGTDYTIGYLTALNTVIDAIDRLRDTSSSHQRISIVEVMGRHCGDLTLM
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHH
AAIAGGCEYVITPETGLNKEALIQNIQDGIAKGKKHAIIAITELMTDVNALAKEIEAETG
HHHHCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCC
RETRATVLGHIQRGGQPGAFDRILASRMGNYGVKLLVEGHGGRCVGIQNEQLVHHDIIDA
CHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHH
IENMRRPEKLELYKVAEELF
HHHCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA