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The map label for this gene is yjeA [H]

Identifier: 209696193

GI number: 209696193

Start: 3019655

End: 3020641

Strand: Reverse

Name: yjeA [H]

Synonym: VSAL_I2787

Alternate gene names: 209696193

Gene position: 3020641-3019655 (Counterclockwise)

Preceding gene: 209696198

Following gene: 209696189

Centisome position: 90.84

GC content: 42.25

Gene sequence:

>987_bases
ATGTATAAAGTTATGACTAGTCAAAACTGGATGCCTAGCGCATCAATTGAACAATTACAGCAACGTGCTGATATCCTTGC
TTTAATCCGTAGATTCTTCTTTGAGCGAAAAGTGATGGAGGTGGATACGCCAGCAATGAGTCACGCAACCGTGACGGATG
TGCACCTTCATACGTTTCAAACTGACTTTGTTGGCCCTGGTTACGCAGATGGCGTACATCTGTATTTTATGACCAGTCCA
GAATTTCATATGAAGCGCTTACTGTCTGCGGGCAGTGGTGCGATTTATCAAATTAATAAAGCCTTTCGTAACGAAGAAGC
TGGGCGTTTTCATAACCCTGAATTTACGATGTTGGAATGGTATCGCCCAACGTTTGATCATCACCAATTGATGGATGAGA
TGAACGAATTGCTTAAACTTGTTTTAAAATGTGATGATGCTGAGCGAATGACGTATCAGCAAGCTTTCATTAACGTGCTT
GGTGTTTGCCCATTAGAAGCGTCGATGGATGAACTTAGAGCTGTAGCGGCACCGTTAGGGTTGTCAGACATTGCTGATGT
TGAAGAAGACCGAGATACATTATTACAATTGCTATTTAGTATGGGCGTTGAGGAGAAAATAGGACAAACGGTGCCAGTTT
GTGTTTATGATTTCCCGGCTTCTCAAGCAGCTCTTGCTAAAATCAATCCTGAAGATCCTAGAGTCGCTGAGCGATTTGAG
GTGTATTTTAAAGGAATTGAATTAGCGAATGGTTTCCATGAACTTGATAATGCGAAGGAACAACTCGCTCGTTTTGAAGA
GGACAATGCGAAACGAAAATCAATGGGTTTGATTGAGCAACCGATCGATTATCATTTGATTGCAGCATTAGAAGCAGGAT
TTCCCGATTGCGCTGGTGTGGCGTTAGGCATTGATCGTTTGATTATGTTAGCAATGGGGGAAAAGCACATCGATCAAGTG
ATGGCTTTTCCTTTTGATAGAGCGTAA

Upstream 100 bases:

>100_bases
TCGGACGTGTATCCCCTTGAAGGTTAAGGGTATTTATAAAGTTAAAAAATAGGTAACCTTGAAATCGGTTATCAGGTATC
ATTTTGGATAAGTTTTTCTA

Downstream 100 bases:

>100_bases
TACCAATCCACAGAAGTGAGTGTGCATTGACTTACAGGGATTAGCTAACGATAAGCCCAGTGCCAATTACCGCCAAACAA
GCACCCAACCACGCAAAACG

Product: lysyl-tRNA synthetase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MYKVMTSQNWMPSASIEQLQQRADILALIRRFFFERKVMEVDTPAMSHATVTDVHLHTFQTDFVGPGYADGVHLYFMTSP
EFHMKRLLSAGSGAIYQINKAFRNEEAGRFHNPEFTMLEWYRPTFDHHQLMDEMNELLKLVLKCDDAERMTYQQAFINVL
GVCPLEASMDELRAVAAPLGLSDIADVEEDRDTLLQLLFSMGVEEKIGQTVPVCVYDFPASQAALAKINPEDPRVAERFE
VYFKGIELANGFHELDNAKEQLARFEEDNAKRKSMGLIEQPIDYHLIAALEAGFPDCAGVALGIDRLIMLAMGEKHIDQV
MAFPFDRA

Sequences:

>Translated_328_residues
MYKVMTSQNWMPSASIEQLQQRADILALIRRFFFERKVMEVDTPAMSHATVTDVHLHTFQTDFVGPGYADGVHLYFMTSP
EFHMKRLLSAGSGAIYQINKAFRNEEAGRFHNPEFTMLEWYRPTFDHHQLMDEMNELLKLVLKCDDAERMTYQQAFINVL
GVCPLEASMDELRAVAAPLGLSDIADVEEDRDTLLQLLFSMGVEEKIGQTVPVCVYDFPASQAALAKINPEDPRVAERFE
VYFKGIELANGFHELDNAKEQLARFEEDNAKRKSMGLIEQPIDYHLIAALEAGFPDCAGVALGIDRLIMLAMGEKHIDQV
MAFPFDRA
>Mature_328_residues
MYKVMTSQNWMPSASIEQLQQRADILALIRRFFFERKVMEVDTPAMSHATVTDVHLHTFQTDFVGPGYADGVHLYFMTSP
EFHMKRLLSAGSGAIYQINKAFRNEEAGRFHNPEFTMLEWYRPTFDHHQLMDEMNELLKLVLKCDDAERMTYQQAFINVL
GVCPLEASMDELRAVAAPLGLSDIADVEEDRDTLLQLLFSMGVEEKIGQTVPVCVYDFPASQAALAKINPEDPRVAERFE
VYFKGIELANGFHELDNAKEQLARFEEDNAKRKSMGLIEQPIDYHLIAALEAGFPDCAGVALGIDRLIMLAMGEKHIDQV
MAFPFDRA

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=332, Percent_Identity=28.9156626506024, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI5031815, Length=332, Percent_Identity=28.9156626506024, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI87082379, Length=322, Percent_Identity=61.4906832298137, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI1790571, Length=323, Percent_Identity=32.1981424148607, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789256, Length=322, Percent_Identity=30.7453416149068, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320242, Length=337, Percent_Identity=29.673590504451, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6324256, Length=349, Percent_Identity=26.9340974212034, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24640851, Length=339, Percent_Identity=25.6637168141593, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24640849, Length=339, Percent_Identity=25.6637168141593, Blast_Score=106, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 37264; Mature: 37264

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKVMTSQNWMPSASIEQLQQRADILALIRRFFFERKVMEVDTPAMSHATVTDVHLHTFQ
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEE
TDFVGPGYADGVHLYFMTSPEFHMKRLLSAGSGAIYQINKAFRNEEAGRFHNPEFTMLEW
CCCCCCCCCCCEEEEEEECCHHHHHHHHHCCCCCEEEEHHHHCCCCCCCCCCCCEEEEEE
YRPTFDHHQLMDEMNELLKLVLKCDDAERMTYQQAFINVLGVCPLEASMDELRAVAAPLG
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
LSDIADVEEDRDTLLQLLFSMGVEEKIGQTVPVCVYDFPASQAALAKINPEDPRVAERFE
HHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEECCCCCHHEEECCCCCCHHHHHHH
VYFKGIELANGFHELDNAKEQLARFEEDNAKRKSMGLIEQPIDYHLIAALEAGFPDCAGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHCCCCHHHHHHHHCCCCCHHHH
ALGIDRLIMLAMGEKHIDQVMAFPFDRA
HHHHHHHHHHHHCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MYKVMTSQNWMPSASIEQLQQRADILALIRRFFFERKVMEVDTPAMSHATVTDVHLHTFQ
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEE
TDFVGPGYADGVHLYFMTSPEFHMKRLLSAGSGAIYQINKAFRNEEAGRFHNPEFTMLEW
CCCCCCCCCCCEEEEEEECCHHHHHHHHHCCCCCEEEEHHHHCCCCCCCCCCCCEEEEEE
YRPTFDHHQLMDEMNELLKLVLKCDDAERMTYQQAFINVLGVCPLEASMDELRAVAAPLG
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
LSDIADVEEDRDTLLQLLFSMGVEEKIGQTVPVCVYDFPASQAALAKINPEDPRVAERFE
HHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEECCCCCHHEEECCCCCCHHHHHHH
VYFKGIELANGFHELDNAKEQLARFEEDNAKRKSMGLIEQPIDYHLIAALEAGFPDCAGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHCCCCHHHHHHHHCCCCCHHHH
ALGIDRLIMLAMGEKHIDQVMAFPFDRA
HHHHHHHHHHHHCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA