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The map label for this gene is hmp [H]

Identifier: 209696176

GI number: 209696176

Start: 2999713

End: 3000897

Strand: Direct

Name: hmp [H]

Synonym: VSAL_I2770

Alternate gene names: 209696176

Gene position: 2999713-3000897 (Clockwise)

Preceding gene: 209696164

Following gene: 209696188

Centisome position: 90.21

GC content: 39.24

Gene sequence:

>1185_bases
ATGCTTAACAAAAATACGATTGATATCATTAAATCTACCATTCCATTACTTGCTCAGACAGGCCCTGCATTAACGGCTCA
TTTCTATGATCGTATGTTTGATCATAACCCTGAGCTAAAAGATATTTTTAACATGAGCAACCAACGCAATGGCGATCAGC
GTGAAGCACTGTTCAATGCTATTTGTGCTTACGCTGGTAACATTGAAAACCTTGCTGTCCTTCTTCCAGCAGTTGAAAAA
ATCGCACACAAACACACGAGTTTTCTAATTACTGCTGAACAATACAATATTGTTGGCGGCCACTTAATTGCAACATTGGA
TGAGATGTTCTCTCCTGGTCAAGAAGTTCTCGATGCATGGACTGAAGCTTATGGCATTCTTGCTAACGTATTTATCCAAC
GTGAAGAACAAATCTACCAAGAAAATGATGTACTTGAGGGTGGATGGCGTGGCTTGCGTGAATTTGAATTAGTTGCAAAA
GAAAAAGAAAGTGAATACATCACTAGTTTCACATTTATGCCGACAGATGATCTAACCGTCTCTTCATATAAACCTGGTCA
GTACATTGGTATTTATTTAAATCCTGAAGAATTCGAAAACCAAGAAGTGCGCCAATACAGTTTATCTTCTGCACCTCAAG
AAAAAACATATCGCATCTCCGTAAAACGAGAAGAAGGTGGTAAAGCCTCTACCTACCTACACGCTTCACTTAATATTGGT
GATAAAGTAAACCTTACTGCACCCGCTGGAGATTTTTTCTTAGACGTTGAGAAATCAACACCAGTTACGCTTATTTCAGC
AGGCGTCGGCTTAACGCCGACTCTTTCTATGTTAGAAACACTCACTAAGCATGAAGAAAAAGTAACTTGGTTGCATGCGA
CGGAAAATGGCTCTCAACATGCGTTCAAGCAACACATTTCTGCTTTAGCTGAAAAGCATGATCATATTTCGGCTTACACT
TGGTATAACCAAGCACAAGAAATGGATAAGATCGGTCAAGATTTTCAATTCAGTGGTTTAATTAACCTAACTGAAGTTAA
AGAAGATATCATTTCTGATGATGGTCACTATTACTTCTGTGGCCCTGTTGGGTTTATGCAGCATGTTGCTAAACAGCTAG
TCGCGCTTGGGGTTTCTGAAGATCGCATTCACTATGAATGTTTTGGTCCTCATAAAGTTCTATAA

Upstream 100 bases:

>100_bases
TCTCTTGACTAAAAGATATATTTTAGATACAACTTATAACATACATTATAAATACAACTTTACATTCATACTATAAATAT
AAATTAAATAGGTCAGCCAA

Downstream 100 bases:

>100_bases
TCTTAAAAATGCAAAAAGCAGCATTGATATATTATCAATGCTGCTTTTTTAGAGCTGATGTTTACATTAAGATTAACTTA
ATGCGAATATTTTAATTACA

Product: nitric oxide dioxygenase

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MLNKNTIDIIKSTIPLLAQTGPALTAHFYDRMFDHNPELKDIFNMSNQRNGDQREALFNAICAYAGNIENLAVLLPAVEK
IAHKHTSFLITAEQYNIVGGHLIATLDEMFSPGQEVLDAWTEAYGILANVFIQREEQIYQENDVLEGGWRGLREFELVAK
EKESEYITSFTFMPTDDLTVSSYKPGQYIGIYLNPEEFENQEVRQYSLSSAPQEKTYRISVKREEGGKASTYLHASLNIG
DKVNLTAPAGDFFLDVEKSTPVTLISAGVGLTPTLSMLETLTKHEEKVTWLHATENGSQHAFKQHISALAEKHDHISAYT
WYNQAQEMDKIGQDFQFSGLINLTEVKEDIISDDGHYYFCGPVGFMQHVAKQLVALGVSEDRIHYECFGPHKVL

Sequences:

>Translated_394_residues
MLNKNTIDIIKSTIPLLAQTGPALTAHFYDRMFDHNPELKDIFNMSNQRNGDQREALFNAICAYAGNIENLAVLLPAVEK
IAHKHTSFLITAEQYNIVGGHLIATLDEMFSPGQEVLDAWTEAYGILANVFIQREEQIYQENDVLEGGWRGLREFELVAK
EKESEYITSFTFMPTDDLTVSSYKPGQYIGIYLNPEEFENQEVRQYSLSSAPQEKTYRISVKREEGGKASTYLHASLNIG
DKVNLTAPAGDFFLDVEKSTPVTLISAGVGLTPTLSMLETLTKHEEKVTWLHATENGSQHAFKQHISALAEKHDHISAYT
WYNQAQEMDKIGQDFQFSGLINLTEVKEDIISDDGHYYFCGPVGFMQHVAKQLVALGVSEDRIHYECFGPHKVL
>Mature_394_residues
MLNKNTIDIIKSTIPLLAQTGPALTAHFYDRMFDHNPELKDIFNMSNQRNGDQREALFNAICAYAGNIENLAVLLPAVEK
IAHKHTSFLITAEQYNIVGGHLIATLDEMFSPGQEVLDAWTEAYGILANVFIQREEQIYQENDVLEGGWRGLREFELVAK
EKESEYITSFTFMPTDDLTVSSYKPGQYIGIYLNPEEFENQEVRQYSLSSAPQEKTYRISVKREEGGKASTYLHASLNIG
DKVNLTAPAGDFFLDVEKSTPVTLISAGVGLTPTLSMLETLTKHEEKVTWLHATENGSQHAFKQHISALAEKHDHISAYT
WYNQAQEMDKIGQDFQFSGLINLTEVKEDIISDDGHYYFCGPVGFMQHVAKQLVALGVSEDRIHYECFGPHKVL

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=396, Percent_Identity=62.1212121212121, Blast_Score=517, Evalue=1e-148,
Organism=Escherichia coli, GI1788104, Length=244, Percent_Identity=25.8196721311475, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1787658, Length=247, Percent_Identity=26.3157894736842, Blast_Score=65, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6321673, Length=410, Percent_Identity=35.609756097561, Blast_Score=219, Evalue=7e-58,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR001709
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 44408; Mature: 44408

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNKNTIDIIKSTIPLLAQTGPALTAHFYDRMFDHNPELKDIFNMSNQRNGDQREALFNA
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHH
ICAYAGNIENLAVLLPAVEKIAHKHTSFLITAEQYNIVGGHLIATLDEMFSPGQEVLDAW
HHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHH
TEAYGILANVFIQREEQIYQENDVLEGGWRGLREFELVAKEKESEYITSFTFMPTDDLTV
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEE
SSYKPGQYIGIYLNPEEFENQEVRQYSLSSAPQEKTYRISVKREEGGKASTYLHASLNIG
ECCCCCCEEEEEECHHHHCCHHHHHHHHCCCCCCCEEEEEEEECCCCCEEEEEEEEECCC
DKVNLTAPAGDFFLDVEKSTPVTLISAGVGLTPTLSMLETLTKHEEKVTWLHATENGSQH
CEEEEECCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCHH
AFKQHISALAEKHDHISAYTWYNQAQEMDKIGQDFQFSGLINLTEVKEDIISDDGHYYFC
HHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCEECCCEEHHHHHHHHHCCCCCEEEE
GPVGFMQHVAKQLVALGVSEDRIHYECFGPHKVL
CHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MLNKNTIDIIKSTIPLLAQTGPALTAHFYDRMFDHNPELKDIFNMSNQRNGDQREALFNA
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHH
ICAYAGNIENLAVLLPAVEKIAHKHTSFLITAEQYNIVGGHLIATLDEMFSPGQEVLDAW
HHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHH
TEAYGILANVFIQREEQIYQENDVLEGGWRGLREFELVAKEKESEYITSFTFMPTDDLTV
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEE
SSYKPGQYIGIYLNPEEFENQEVRQYSLSSAPQEKTYRISVKREEGGKASTYLHASLNIG
ECCCCCCEEEEEECHHHHCCHHHHHHHHCCCCCCCEEEEEEEECCCCCEEEEEEEEECCC
DKVNLTAPAGDFFLDVEKSTPVTLISAGVGLTPTLSMLETLTKHEEKVTWLHATENGSQH
CEEEEECCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCHH
AFKQHISALAEKHDHISAYTWYNQAQEMDKIGQDFQFSGLINLTEVKEDIISDDGHYYFC
HHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCEECCCEEHHHHHHHHHCCCCCEEEE
GPVGFMQHVAKQLVALGVSEDRIHYECFGPHKVL
CHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA