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The map label for this gene is argG [H]

Identifier: 209696164

GI number: 209696164

Start: 2989206

End: 2990417

Strand: Direct

Name: argG [H]

Synonym: VSAL_I2758

Alternate gene names: 209696164

Gene position: 2989206-2990417 (Clockwise)

Preceding gene: 209696163

Following gene: 209696176

Centisome position: 89.9

GC content: 42.24

Gene sequence:

>1212_bases
ATGAATAAGAAAGTAAACGTAAACAAGGTCGTTGTTGCATACTCTGGTGGTCTAGATACATCTGTGATTATCCCGTGGTT
AAAAGAAAACTACGATTGTGAAGTGATTGCATTTGTTGCTGATGTGGGTCAAGGGGCTGAAGAGTTAGAAGGTATTGAAG
CTAAAGCGATTGCTTCCGGTGCATCTGAGTGCTATGTGGCTGACTTAAAAGAAGAAATGGTCTCTGAGTACATTTTTCCT
ACGTTAAAAACAGGCGCTCTTTATGAGGGTAAATACCTACTTGGTACGTCAATGGCTCGTCCTATTATCGCAAAAGCACA
AGTTGAAGTGGCGCGTAATGTTGGCGCAGATGCACTTTGCCATGGCTGCACAGGTAAAGGTAATGACCAAATCCGTTTTG
AAGGTGCATTCGCAGCATTAGCACCAGATCTACACGTAATTGCACCATGGCGTGAGTGGGATTTAGTGAGCCGTGAAGAG
TGTCTTGATTACCTTGCTGAACGTAATATCCCTTGTACTGCTTCACTTACAAAGATCTACTCTCGTGATGCAAACGCATG
GCATATCTCAACTGAAGGTGGTGTTCTTGAAGATACATGGAACGAGCCAAACGAAGATTGCTGGGCATGGACTGTCGATC
CAGAGCAAGCGCCAAATGAATCAGAAACAGTATCAATAAAAGTTGAAAAAGGCGCGGTTGTTGCCGTTGATGGTAAAGCG
ATGACCCCTTATGAAGTGGTTGTTTATCTAAACGAGAAAGGAATAAAACACGGTGTTGGTCGTATCGATATCGTTGAAAA
CCGCCTTGTTGGAATGAAATCTCGTGGTTGTTATGAAACTCCAGGTGGCACGATTATCAATGAAGCACTACGTGCGGTTG
AGCAATTGGTGTTAGATAAAGCGTCATTTGAATTTCGTGAAGAGTTAGGGATCAAAGCCTCTCATCTTGTTTATGATGGT
CGTTGGTTTACACCATTGTGTAAATCAATCTTAGCGGCAACAGAAGAACTGGCTCAAGACGTTAATGGTGATGTTGTTAT
TAAGCTTTATAAAGGTCAAGCGACCGTAACTCAAAAGCGCTCTGATAATAGCTTGTATTCAGAAGAGTTTGCTACTTTTG
GTGCTGATGAAGTTTACGACCAAAGCCATGCAGAAGGTTTTATCCGTCTTTACTCATTATCAAGCCGTATTCGTGCTCTA
AATAGTAAGTAA

Upstream 100 bases:

>100_bases
GGAAATAGCATCGGCACACAGTTTCAGCCTTAATTGGCATTGAATAGAATTAACTTTATAGAATACAGTCGCCGCGAGTA
TGCGGATCAAGGAGAATAGA

Downstream 100 bases:

>100_bases
TTACTATTATGTAACAACTGTTTGATAATTTGTTTTACACTAGTCGAAGTTCATTTAATGATTAAATGAACTTCGATTTT
TTTTGTCTACATTATTATAA

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MNKKVNVNKVVVAYSGGLDTSVIIPWLKENYDCEVIAFVADVGQGAEELEGIEAKAIASGASECYVADLKEEMVSEYIFP
TLKTGALYEGKYLLGTSMARPIIAKAQVEVARNVGADALCHGCTGKGNDQIRFEGAFAALAPDLHVIAPWREWDLVSREE
CLDYLAERNIPCTASLTKIYSRDANAWHISTEGGVLEDTWNEPNEDCWAWTVDPEQAPNESETVSIKVEKGAVVAVDGKA
MTPYEVVVYLNEKGIKHGVGRIDIVENRLVGMKSRGCYETPGGTIINEALRAVEQLVLDKASFEFREELGIKASHLVYDG
RWFTPLCKSILAATEELAQDVNGDVVIKLYKGQATVTQKRSDNSLYSEEFATFGADEVYDQSHAEGFIRLYSLSSRIRAL
NSK

Sequences:

>Translated_403_residues
MNKKVNVNKVVVAYSGGLDTSVIIPWLKENYDCEVIAFVADVGQGAEELEGIEAKAIASGASECYVADLKEEMVSEYIFP
TLKTGALYEGKYLLGTSMARPIIAKAQVEVARNVGADALCHGCTGKGNDQIRFEGAFAALAPDLHVIAPWREWDLVSREE
CLDYLAERNIPCTASLTKIYSRDANAWHISTEGGVLEDTWNEPNEDCWAWTVDPEQAPNESETVSIKVEKGAVVAVDGKA
MTPYEVVVYLNEKGIKHGVGRIDIVENRLVGMKSRGCYETPGGTIINEALRAVEQLVLDKASFEFREELGIKASHLVYDG
RWFTPLCKSILAATEELAQDVNGDVVIKLYKGQATVTQKRSDNSLYSEEFATFGADEVYDQSHAEGFIRLYSLSSRIRAL
NSK
>Mature_403_residues
MNKKVNVNKVVVAYSGGLDTSVIIPWLKENYDCEVIAFVADVGQGAEELEGIEAKAIASGASECYVADLKEEMVSEYIFP
TLKTGALYEGKYLLGTSMARPIIAKAQVEVARNVGADALCHGCTGKGNDQIRFEGAFAALAPDLHVIAPWREWDLVSREE
CLDYLAERNIPCTASLTKIYSRDANAWHISTEGGVLEDTWNEPNEDCWAWTVDPEQAPNESETVSIKVEKGAVVAVDGKA
MTPYEVVVYLNEKGIKHGVGRIDIVENRLVGMKSRGCYETPGGTIINEALRAVEQLVLDKASFEFREELGIKASHLVYDG
RWFTPLCKSILAATEELAQDVNGDVVIKLYKGQATVTQKRSDNSLYSEEFATFGADEVYDQSHAEGFIRLYSLSSRIRAL
NSK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=394, Percent_Identity=43.1472081218274, Blast_Score=306, Evalue=3e-83,
Organism=Homo sapiens, GI16950633, Length=394, Percent_Identity=43.1472081218274, Blast_Score=306, Evalue=3e-83,
Organism=Escherichia coli, GI1789563, Length=403, Percent_Identity=26.0545905707196, Blast_Score=126, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6324514, Length=400, Percent_Identity=39.25, Blast_Score=278, Evalue=1e-75,
Organism=Drosophila melanogaster, GI21358151, Length=408, Percent_Identity=39.9509803921569, Blast_Score=277, Evalue=1e-74,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 44340; Mature: 44340

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKVNVNKVVVAYSGGLDTSVIIPWLKENYDCEVIAFVADVGQGAEELEGIEAKAIASG
CCCCCCCEEEEEEEECCCCCEEEEEHHCCCCCCEEEEEEEHHCCCHHHHCCCCHHHHHCC
ASECYVADLKEEMVSEYIFPTLKTGALYEGKYLLGTSMARPIIAKAQVEVARNVGADALC
CCHHHHHHHHHHHHHHHHCCCCCCCCEECCCEEECCHHCCCHHHHHHHHHHHCCCHHHHE
HGCTGKGNDQIRFEGAFAALAPDLHVIAPWREWDLVSREECLDYLAERNIPCTASLTKIY
ECCCCCCCCEEEEECCHHHHCCCCEEECCCHHCCCCCHHHHHHHHHHCCCCCCHHHHHHH
SRDANAWHISTEGGVLEDTWNEPNEDCWAWTVDPEQAPNESETVSIKVEKGAVVAVDGKA
CCCCCEEEEECCCCEEECCCCCCCCCEEEEEECHHCCCCCCCEEEEEEECCEEEEECCCC
MTPYEVVVYLNEKGIKHGVGRIDIVENRLVGMKSRGCYETPGGTIINEALRAVEQLVLDK
CCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ASFEFREELGIKASHLVYDGRWFTPLCKSILAATEELAQDVNGDVVIKLYKGQATVTQKR
HHHHHHHHHCCEEHEEEECCEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCC
SDNSLYSEEFATFGADEVYDQSHAEGFIRLYSLSSRIRALNSK
CCCCHHHHHHHHCCCHHHHHHHHHHHEEEEEHHHHHHHHHCCC
>Mature Secondary Structure
MNKKVNVNKVVVAYSGGLDTSVIIPWLKENYDCEVIAFVADVGQGAEELEGIEAKAIASG
CCCCCCCEEEEEEEECCCCCEEEEEHHCCCCCCEEEEEEEHHCCCHHHHCCCCHHHHHCC
ASECYVADLKEEMVSEYIFPTLKTGALYEGKYLLGTSMARPIIAKAQVEVARNVGADALC
CCHHHHHHHHHHHHHHHHCCCCCCCCEECCCEEECCHHCCCHHHHHHHHHHHCCCHHHHE
HGCTGKGNDQIRFEGAFAALAPDLHVIAPWREWDLVSREECLDYLAERNIPCTASLTKIY
ECCCCCCCCEEEEECCHHHHCCCCEEECCCHHCCCCCHHHHHHHHHHCCCCCCHHHHHHH
SRDANAWHISTEGGVLEDTWNEPNEDCWAWTVDPEQAPNESETVSIKVEKGAVVAVDGKA
CCCCCEEEEECCCCEEECCCCCCCCCEEEEEECHHCCCCCCCEEEEEEECCEEEEECCCC
MTPYEVVVYLNEKGIKHGVGRIDIVENRLVGMKSRGCYETPGGTIINEALRAVEQLVLDK
CCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ASFEFREELGIKASHLVYDGRWFTPLCKSILAATEELAQDVNGDVVIKLYKGQATVTQKR
HHHHHHHHHCCEEHEEEECCEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCC
SDNSLYSEEFATFGADEVYDQSHAEGFIRLYSLSSRIRALNSK
CCCCHHHHHHHHCCCHHHHHHHHHHHEEEEEHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA