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The map label for this gene is priA [H]

Identifier: 209696149

GI number: 209696149

Start: 2968477

End: 2970678

Strand: Reverse

Name: priA [H]

Synonym: VSAL_I2743

Alternate gene names: 209696149

Gene position: 2970678-2968477 (Counterclockwise)

Preceding gene: 209696152

Following gene: 209696148

Centisome position: 89.34

GC content: 43.55

Gene sequence:

>2202_bases
ATGTCCTATTCAATTGCACACGTTTCACTGCCCGTTCCTCTTGACCGCACCTTTGATTACTTAATTAAAGCCGGACAACA
CCCTGTCATTGGGGGCCGTGTAAAAGTGCCTTTTGGTCGTCAGCGCCTTATCGGTATAGTCACAAAATTAAGCAATGACT
CTGAATTTGCTCGTGAGAAATTAAAAGGATTAGACTCCGTACTCGATAGCACTTCTCTCTGGCCTCCTCACCTTTATAAG
CTTCTTAATTGGAGCAGCACTTATTATCAATACCCATTAGGGGAAACCTTAGCGAATGCGATGCCATCGTGGCTACGTAA
AGGCAGAGAAGCCAGTTTTGCTGCACTAAAAGCGTGGCAAATTACTGAGCTAGGAAAAAATCAAGATCTCAACAAACTGA
CTCGAGCACCGAAACAAGCCAAGGCCCTTAACCTTGTTCGTCACCAACAATGCAGTCACGAACAAATGCTTGAAGAAGAC
ATTACGGCTGCAACCTTAAAAGCCGTCGTGGATAAAGGATGGATAGAAGAAATCTCTCTTGCTCCAAAACCAACTTATTG
GCAACGTAATGTCGAAATAACACAAGAAAAGCCACAGTTAAATGAAGAGCAAGCTATTGCTATTGCCACGGTTAATGCAA
ACCCTGAATTTGGTTGTTACTTATTAGATGGCGTTACGGGATCGGGTAAAACGGAAGTGTATCTCCAGTTATTAGAGCCT
ATTTTACACGCAGGCCGACAAGCACTTATTCTTGTTCCTGAAATAGGATTAACCCCACAAACCATTAATCGCTTTAAGCG
TCGTTTTAATGTGCCTGTCGACATGATCCATTCTGCACTTAATGATACCGAACGTTTAAATGCATGGTTAGCAGCACGAG
ATAACCATGCCGGCATCATTATAGGCACTCGCTCAGCACTTTTTACCCCATTTAATGATCTCGGTATCATCATTGTCGAT
GAAGAACACGATGCCTCTTATAAACAGCAAGACAGCATGAGATATCACGCCCGAGATCTGGCAGTAATGCGAGCAAATTT
AGACAATATTCCGATTGTGCTAGGCTCAGCAACGCCAGCTCTAGAGACGTTACATAATGCTAAGGTCGGTAAATACCACC
ATCTCACATTAACCAAACGCGCGGGTACTGCAATCCCAGCTCGACATGGTGTGTTAGATGTCAAAGGCTTGTATCTAAAC
AGTGGATTATCTGCACCTTTAATTGCTCAAATGAGAAAACATTTATCTGAAGGCAATCAGGTGATGTTATTTTTGAATCG
CCGAGGATTCTCCCCAGCCCTAATGTGTCACGAATGTGGCTGGATGGCCGAATGTAAACGCTGTGATGCTTATTATACGT
TCCACCAACACAGTAATGAAATTCGCTGCCACCACTGTGGTTCACAGCAGCCAATAATGCATCAATGCCAAGGCTGTGGA
TCCACTCAGCTTATTTCTGTAGGGGTTGGTACCGAACAGCTCGAAACTCAACTAATGACCCTCTTTCCTGAATACAAAAC
CGTTCGTATCGATCGAGACAGTACTCGCAGAAAAGGCAGCTTAGAAAACTATCTAGACGCAATTAAAAACAACGAATACC
AAATCCTTATTGGCACTCAAATGCTAGCAAAAGGGCACCATTTTCCTAACGTAACCCTTGTCGGTTTAATTGATGTAGAT
AGTTCATTGTTTAGTAGTGATTTTCGAGCTCCTGAACGTTTAGCTCAGTTGTTTATCCAAGTCGCTGGACGCGCAGGGCG
AGCAAGTAAACCAGGGGAAGTGATCTTACAAACTCACCACCCTGAACACTCTCTTTTACAAAGCCTTCTACGTCATGGTT
ATGGTCACTTTGCTGAACATGCGTTAAATGAGCGCAAATTCGCTCAGCTCCCTCCTTATACGCATTTATCTCTCTTTAGA
GCAGAGTCCAATAACAATGACAATGTCGAGCAATTCTTACGACAAGTCCGTGATATTTTAGAAAGTAACCCTCAATATCA
TGACGCCACACCAGTGCTAGGGCCTATTCCGGCACCTTTAGCACGTCGAGCCGGAAAATACCGTTGGCAACTGCTCTTTC
AAGCGCCAAATCGTACCGTAATGCAATCAATGTTACGTGGTGCAAAAACCGCGATTGAGCTATTACCATTAGCTAAAAAA
GTACGTTGGACTTTAGACATTGAGCCACAAGACCTTAGTTAA

Upstream 100 bases:

>100_bases
TTCCACCGATTATTTTACTCCTTTGCGATATTTTTATCTAAGAGTAGACTATTCATCCTATTCATCCTATTCATCCTATT
ATTCCTGATTAATGTTGGCT

Downstream 100 bases:

>100_bases
TCACGCTTTTTTAATTTCAAAACTAAGACTTACTCAGGAAATACCCTCTCATGTGAGGGACATCACAATATAGATGTAAC
TTTTGTTAAATGCGCCGTAT

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 733; Mature: 732

Protein sequence:

>733_residues
MSYSIAHVSLPVPLDRTFDYLIKAGQHPVIGGRVKVPFGRQRLIGIVTKLSNDSEFAREKLKGLDSVLDSTSLWPPHLYK
LLNWSSTYYQYPLGETLANAMPSWLRKGREASFAALKAWQITELGKNQDLNKLTRAPKQAKALNLVRHQQCSHEQMLEED
ITAATLKAVVDKGWIEEISLAPKPTYWQRNVEITQEKPQLNEEQAIAIATVNANPEFGCYLLDGVTGSGKTEVYLQLLEP
ILHAGRQALILVPEIGLTPQTINRFKRRFNVPVDMIHSALNDTERLNAWLAARDNHAGIIIGTRSALFTPFNDLGIIIVD
EEHDASYKQQDSMRYHARDLAVMRANLDNIPIVLGSATPALETLHNAKVGKYHHLTLTKRAGTAIPARHGVLDVKGLYLN
SGLSAPLIAQMRKHLSEGNQVMLFLNRRGFSPALMCHECGWMAECKRCDAYYTFHQHSNEIRCHHCGSQQPIMHQCQGCG
STQLISVGVGTEQLETQLMTLFPEYKTVRIDRDSTRRKGSLENYLDAIKNNEYQILIGTQMLAKGHHFPNVTLVGLIDVD
SSLFSSDFRAPERLAQLFIQVAGRAGRASKPGEVILQTHHPEHSLLQSLLRHGYGHFAEHALNERKFAQLPPYTHLSLFR
AESNNNDNVEQFLRQVRDILESNPQYHDATPVLGPIPAPLARRAGKYRWQLLFQAPNRTVMQSMLRGAKTAIELLPLAKK
VRWTLDIEPQDLS

Sequences:

>Translated_733_residues
MSYSIAHVSLPVPLDRTFDYLIKAGQHPVIGGRVKVPFGRQRLIGIVTKLSNDSEFAREKLKGLDSVLDSTSLWPPHLYK
LLNWSSTYYQYPLGETLANAMPSWLRKGREASFAALKAWQITELGKNQDLNKLTRAPKQAKALNLVRHQQCSHEQMLEED
ITAATLKAVVDKGWIEEISLAPKPTYWQRNVEITQEKPQLNEEQAIAIATVNANPEFGCYLLDGVTGSGKTEVYLQLLEP
ILHAGRQALILVPEIGLTPQTINRFKRRFNVPVDMIHSALNDTERLNAWLAARDNHAGIIIGTRSALFTPFNDLGIIIVD
EEHDASYKQQDSMRYHARDLAVMRANLDNIPIVLGSATPALETLHNAKVGKYHHLTLTKRAGTAIPARHGVLDVKGLYLN
SGLSAPLIAQMRKHLSEGNQVMLFLNRRGFSPALMCHECGWMAECKRCDAYYTFHQHSNEIRCHHCGSQQPIMHQCQGCG
STQLISVGVGTEQLETQLMTLFPEYKTVRIDRDSTRRKGSLENYLDAIKNNEYQILIGTQMLAKGHHFPNVTLVGLIDVD
SSLFSSDFRAPERLAQLFIQVAGRAGRASKPGEVILQTHHPEHSLLQSLLRHGYGHFAEHALNERKFAQLPPYTHLSLFR
AESNNNDNVEQFLRQVRDILESNPQYHDATPVLGPIPAPLARRAGKYRWQLLFQAPNRTVMQSMLRGAKTAIELLPLAKK
VRWTLDIEPQDLS
>Mature_732_residues
SYSIAHVSLPVPLDRTFDYLIKAGQHPVIGGRVKVPFGRQRLIGIVTKLSNDSEFAREKLKGLDSVLDSTSLWPPHLYKL
LNWSSTYYQYPLGETLANAMPSWLRKGREASFAALKAWQITELGKNQDLNKLTRAPKQAKALNLVRHQQCSHEQMLEEDI
TAATLKAVVDKGWIEEISLAPKPTYWQRNVEITQEKPQLNEEQAIAIATVNANPEFGCYLLDGVTGSGKTEVYLQLLEPI
LHAGRQALILVPEIGLTPQTINRFKRRFNVPVDMIHSALNDTERLNAWLAARDNHAGIIIGTRSALFTPFNDLGIIIVDE
EHDASYKQQDSMRYHARDLAVMRANLDNIPIVLGSATPALETLHNAKVGKYHHLTLTKRAGTAIPARHGVLDVKGLYLNS
GLSAPLIAQMRKHLSEGNQVMLFLNRRGFSPALMCHECGWMAECKRCDAYYTFHQHSNEIRCHHCGSQQPIMHQCQGCGS
TQLISVGVGTEQLETQLMTLFPEYKTVRIDRDSTRRKGSLENYLDAIKNNEYQILIGTQMLAKGHHFPNVTLVGLIDVDS
SLFSSDFRAPERLAQLFIQVAGRAGRASKPGEVILQTHHPEHSLLQSLLRHGYGHFAEHALNERKFAQLPPYTHLSLFRA
ESNNNDNVEQFLRQVRDILESNPQYHDATPVLGPIPAPLARRAGKYRWQLLFQAPNRTVMQSMLRGAKTAIELLPLAKKV
RWTLDIEPQDLS

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=727, Percent_Identity=53.9202200825309, Blast_Score=803, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 82494; Mature: 82362

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYSIAHVSLPVPLDRTFDYLIKAGQHPVIGGRVKVPFGRQRLIGIVTKLSNDSEFAREK
CCEEEEEEEECCCCHHHHHHHHHCCCCCEECCEEECCCCHHHHHHHHHHCCCCHHHHHHH
LKGLDSVLDSTSLWPPHLYKLLNWSSTYYQYPLGETLANAMPSWLRKGREASFAALKAWQ
HHHHHHHHHCCCCCCHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHEE
ITELGKNQDLNKLTRAPKQAKALNLVRHQQCSHEQMLEEDITAATLKAVVDKGWIEEISL
HHHCCCCCCHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHCC
APKPTYWQRNVEITQEKPQLNEEQAIAIATVNANPEFGCYLLDGVTGSGKTEVYLQLLEP
CCCCCCCCCCCEEECCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHH
ILHAGRQALILVPEIGLTPQTINRFKRRFNVPVDMIHSALNDTERLNAWLAARDNHAGII
HHHCCCEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHEEECCCCCEEE
IGTRSALFTPFNDLGIIIVDEEHDASYKQQDSMRYHARDLAVMRANLDNIPIVLGSATPA
EECCHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHH
LETLHNAKVGKYHHLTLTKRAGTAIPARHGVLDVKGLYLNSGLSAPLIAQMRKHLSEGNQ
HHHHHCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCE
VMLFLNRRGFSPALMCHECGWMAECKRCDAYYTFHQHSNEIRCHHCGSQQPIMHQCQGCG
EEEEEECCCCCHHHHHHHCCCHHHHHHCCCEEEEECCCCCEEEEECCCCCCHHHHHCCCC
STQLISVGVGTEQLETQLMTLFPEYKTVRIDRDSTRRKGSLENYLDAIKNNEYQILIGTQ
CCEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCEEEEEEHH
MLAKGHHFPNVTLVGLIDVDSSLFSSDFRAPERLAQLFIQVAGRAGRASKPGEVILQTHH
HHHCCCCCCCEEEEEEEECCHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECC
PEHSLLQSLLRHGYGHFAEHALNERKFAQLPPYTHLSLFRAESNNNDNVEQFLRQVRDIL
CHHHHHHHHHHCCCHHHHHHHHCCCHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHH
ESNPQYHDATPVLGPIPAPLARRAGKYRWQLLFQAPNRTVMQSMLRGAKTAIELLPLAKK
HCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
VRWTLDIEPQDLS
EEEEEECCCCCCC
>Mature Secondary Structure 
SYSIAHVSLPVPLDRTFDYLIKAGQHPVIGGRVKVPFGRQRLIGIVTKLSNDSEFAREK
CEEEEEEEECCCCHHHHHHHHHCCCCCEECCEEECCCCHHHHHHHHHHCCCCHHHHHHH
LKGLDSVLDSTSLWPPHLYKLLNWSSTYYQYPLGETLANAMPSWLRKGREASFAALKAWQ
HHHHHHHHHCCCCCCHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHEE
ITELGKNQDLNKLTRAPKQAKALNLVRHQQCSHEQMLEEDITAATLKAVVDKGWIEEISL
HHHCCCCCCHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHCC
APKPTYWQRNVEITQEKPQLNEEQAIAIATVNANPEFGCYLLDGVTGSGKTEVYLQLLEP
CCCCCCCCCCCEEECCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHH
ILHAGRQALILVPEIGLTPQTINRFKRRFNVPVDMIHSALNDTERLNAWLAARDNHAGII
HHHCCCEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHEEECCCCCEEE
IGTRSALFTPFNDLGIIIVDEEHDASYKQQDSMRYHARDLAVMRANLDNIPIVLGSATPA
EECCHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHH
LETLHNAKVGKYHHLTLTKRAGTAIPARHGVLDVKGLYLNSGLSAPLIAQMRKHLSEGNQ
HHHHHCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCE
VMLFLNRRGFSPALMCHECGWMAECKRCDAYYTFHQHSNEIRCHHCGSQQPIMHQCQGCG
EEEEEECCCCCHHHHHHHCCCHHHHHHCCCEEEEECCCCCEEEEECCCCCCHHHHHCCCC
STQLISVGVGTEQLETQLMTLFPEYKTVRIDRDSTRRKGSLENYLDAIKNNEYQILIGTQ
CCEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCEEEEEEHH
MLAKGHHFPNVTLVGLIDVDSSLFSSDFRAPERLAQLFIQVAGRAGRASKPGEVILQTHH
HHHCCCCCCCEEEEEEEECCHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECC
PEHSLLQSLLRHGYGHFAEHALNERKFAQLPPYTHLSLFRAESNNNDNVEQFLRQVRDIL
CHHHHHHHHHHCCCHHHHHHHHCCCHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHH
ESNPQYHDATPVLGPIPAPLARRAGKYRWQLLFQAPNRTVMQSMLRGAKTAIELLPLAKK
HCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
VRWTLDIEPQDLS
EEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]