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The map label for this gene is ycdT [H]

Identifier: 209696111

GI number: 209696111

Start: 2927417

End: 2929306

Strand: Reverse

Name: ycdT [H]

Synonym: VSAL_I2704

Alternate gene names: 209696111

Gene position: 2929306-2927417 (Counterclockwise)

Preceding gene: 209696112

Following gene: 209696110

Centisome position: 88.1

GC content: 34.13

Gene sequence:

>1890_bases
TTGAGTTCAACTGACATGAATAAGAAAAGTTTTAAAATAGTAGCTTATTCTAGCTGTGTTTATCTTGTGTTATCAATGAT
AACGCTGGTGTATTTTTATAGTGAATATCAGAAAGAAAAACTGGTTATTATTGAGCAAGTTCAAAATGAATCAACTCGAC
AGTTAGCTTATACACATCATGAATTTGATGAGTTATTAACTCGTATCCAAAATACATTAAGCTTACTCTCTGACAGCCGA
CCTGTTTATGATTATTTTTTATATCCTTCCAATCGTGCTAAAGCAGCCGTAGAAAGCTCATGGGTTAATTTGGTTAAAGC
TGAAAAGTTCTTCTCTCAATTAGAGCTTATCGATAACCAAGGGATTGCTTTATTTAGCTTATCTTATAACGCCATCAATA
ATGAGACATCTAAACATAATTTGCCCTTAACTGTCGTGAATCACTCCTTATTAACAACGTTATCCCAATTAGCTGCCGGA
CAAGTATTTTCGGAAGGGGTGGTCTTAAATGAAAAGTTAGCGACGTCGGGTGTTTATAAGCCAGTCATGCAAATTATTAT
TCCAATTGAAGTGTTAGGATCTCGTAAAGGGTATGTTGTTGCTGAACTGAGTGAGACGAGAATTGAAAATGTGATTAAAT
TTTCTCCTACGCTTAATATCTCTCCCTCTATCGTTAATCAAGATGGTTATTATTTATTAACGAATAAGCGAAATAAAATG
TTTGGTCATCTTGTTAATGGAAGAGAAGATCAAACATTAAAGGCTGAAAATTCGGAGTTATGGCGCTTAATGACTACTCA
AAAAACAGGCAGTGCTTACATTGATAAAAATATTTTTTCGTTTTATAAATTCTCTCTTAATCAAAAAGTGCCAAATAAAG
AAGTGTACTTTTTATTGGATTACTCTGAAGAAAGCTTGAATGTTTTATTTCAAGAAGCGAAGTCTGAATTAATCGAAGAA
ACAACGATTGTATTATTGTTATTAAGCTTAATCGTACTTCCTTGTGGGGTCGCTTTAACATTATGGGATAAATCAAATAT
TGAAAGCCAACTTGCAAAGTCAGCTCTTGAGGGGATGACGGCTGTGGTGATCACAGATAAATATCAACGAATTATAAAAG
TAAATTCAGCGTTTTCTTTAATTAGTGGATGCTGTGCATCAGAGACGATAGGAAAAACCACATTTGATGTATTGAAGTCA
GGTGTTGAACATTCAGAAAAGCGAGTTCAGAACTGGAAACAAGTCGAAGAACTGGGTGTTTGGCAAGGTGAAATCGAAGC
ATTAAATAAAGACGAAAAAAAATTAAGCTTGCTGGTAAGAATTCAAGTTGTTAAAAATAAGCGTAAAGACATTCAATATT
ATATTTTGTCTTATATTGATATTACTCAACGAAAATTATTAGAAAAAGAATTGCGTTATTTAAGTGAAAAAGACAGTTTA
TCGGATTGTTGGAATCGCAGAAAATTCGAGTTTGAATTAGAGAATCAGACGTTATTAACGAATCGTTATTACCCAGTACA
TCAATGTTGTTTAGCCATTCTTGATATTGACTGGTTTAAAAGAATTAATGACACTAAAGGCCATGACGTTGGTGATAAGG
TCATTTTTCTTGTCGCAGGTATACTGCAAAAAGGCTCTAGAGATGGAGGAGAAGAGTTTGCGATAATTATGCCTCATACT
GAGTTGAAAGATGCAGAGAGGGTCGTCAATCGCTTACGTGTCGCGGTTTCTCAATTGGAAGAATATCAATTAACGATTAG
TGGTGGTTTGGCTGATATAGGTATTGCAGGTAATGATGCTTATAAATGTGCCGACTTAGCATTGTATGAATCAAAAACCA
ATGGACGTAATCTTATTTCTATTTGTAGTGATTTAGGGGATTTGGCTTAG

Upstream 100 bases:

>100_bases
ATTTACAATTCAATTAGATAGATTTATAAATCAAGCCTTCTTTTTGAGAAGGCTTGATTGTTTATATAATGATAAAAATA
TAGAATGACGCCCTGTATGT

Downstream 100 bases:

>100_bases
GTACATTATCGCTTATTAGACTAAAGTAGTAAGACGGCTATACTTATTAGTATCGTTTAATTTGTCTACAGCAGGACGCT
GATATTGCAAAGTGATGCGC

Product: membrane associated GGDEF protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 629; Mature: 628

Protein sequence:

>629_residues
MSSTDMNKKSFKIVAYSSCVYLVLSMITLVYFYSEYQKEKLVIIEQVQNESTRQLAYTHHEFDELLTRIQNTLSLLSDSR
PVYDYFLYPSNRAKAAVESSWVNLVKAEKFFSQLELIDNQGIALFSLSYNAINNETSKHNLPLTVVNHSLLTTLSQLAAG
QVFSEGVVLNEKLATSGVYKPVMQIIIPIEVLGSRKGYVVAELSETRIENVIKFSPTLNISPSIVNQDGYYLLTNKRNKM
FGHLVNGREDQTLKAENSELWRLMTTQKTGSAYIDKNIFSFYKFSLNQKVPNKEVYFLLDYSEESLNVLFQEAKSELIEE
TTIVLLLLSLIVLPCGVALTLWDKSNIESQLAKSALEGMTAVVITDKYQRIIKVNSAFSLISGCCASETIGKTTFDVLKS
GVEHSEKRVQNWKQVEELGVWQGEIEALNKDEKKLSLLVRIQVVKNKRKDIQYYILSYIDITQRKLLEKELRYLSEKDSL
SDCWNRRKFEFELENQTLLTNRYYPVHQCCLAILDIDWFKRINDTKGHDVGDKVIFLVAGILQKGSRDGGEEFAIIMPHT
ELKDAERVVNRLRVAVSQLEEYQLTISGGLADIGIAGNDAYKCADLALYESKTNGRNLISICSDLGDLA

Sequences:

>Translated_629_residues
MSSTDMNKKSFKIVAYSSCVYLVLSMITLVYFYSEYQKEKLVIIEQVQNESTRQLAYTHHEFDELLTRIQNTLSLLSDSR
PVYDYFLYPSNRAKAAVESSWVNLVKAEKFFSQLELIDNQGIALFSLSYNAINNETSKHNLPLTVVNHSLLTTLSQLAAG
QVFSEGVVLNEKLATSGVYKPVMQIIIPIEVLGSRKGYVVAELSETRIENVIKFSPTLNISPSIVNQDGYYLLTNKRNKM
FGHLVNGREDQTLKAENSELWRLMTTQKTGSAYIDKNIFSFYKFSLNQKVPNKEVYFLLDYSEESLNVLFQEAKSELIEE
TTIVLLLLSLIVLPCGVALTLWDKSNIESQLAKSALEGMTAVVITDKYQRIIKVNSAFSLISGCCASETIGKTTFDVLKS
GVEHSEKRVQNWKQVEELGVWQGEIEALNKDEKKLSLLVRIQVVKNKRKDIQYYILSYIDITQRKLLEKELRYLSEKDSL
SDCWNRRKFEFELENQTLLTNRYYPVHQCCLAILDIDWFKRINDTKGHDVGDKVIFLVAGILQKGSRDGGEEFAIIMPHT
ELKDAERVVNRLRVAVSQLEEYQLTISGGLADIGIAGNDAYKCADLALYESKTNGRNLISICSDLGDLA
>Mature_628_residues
SSTDMNKKSFKIVAYSSCVYLVLSMITLVYFYSEYQKEKLVIIEQVQNESTRQLAYTHHEFDELLTRIQNTLSLLSDSRP
VYDYFLYPSNRAKAAVESSWVNLVKAEKFFSQLELIDNQGIALFSLSYNAINNETSKHNLPLTVVNHSLLTTLSQLAAGQ
VFSEGVVLNEKLATSGVYKPVMQIIIPIEVLGSRKGYVVAELSETRIENVIKFSPTLNISPSIVNQDGYYLLTNKRNKMF
GHLVNGREDQTLKAENSELWRLMTTQKTGSAYIDKNIFSFYKFSLNQKVPNKEVYFLLDYSEESLNVLFQEAKSELIEET
TIVLLLLSLIVLPCGVALTLWDKSNIESQLAKSALEGMTAVVITDKYQRIIKVNSAFSLISGCCASETIGKTTFDVLKSG
VEHSEKRVQNWKQVEELGVWQGEIEALNKDEKKLSLLVRIQVVKNKRKDIQYYILSYIDITQRKLLEKELRYLSEKDSLS
DCWNRRKFEFELENQTLLTNRYYPVHQCCLAILDIDWFKRINDTKGHDVGDKVIFLVAGILQKGSRDGGEEFAIIMPHTE
LKDAERVVNRLRVAVSQLEEYQLTISGGLADIGIAGNDAYKCADLALYESKTNGRNLISICSDLGDLA

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=162, Percent_Identity=35.1851851851852, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786584, Length=183, Percent_Identity=34.4262295081967, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI87081881, Length=180, Percent_Identity=33.8888888888889, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788381, Length=181, Percent_Identity=24.8618784530387, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1787541, Length=276, Percent_Identity=26.0869565217391, Blast_Score=71, Evalue=3e-13,
Organism=Escherichia coli, GI1787802, Length=175, Percent_Identity=30.2857142857143, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87082007, Length=188, Percent_Identity=27.1276595744681, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 71458; Mature: 71327

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS50113 PAC ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTDMNKKSFKIVAYSSCVYLVLSMITLVYFYSEYQKEKLVIIEQVQNESTRQLAYTHH
CCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHCCCCHHHHHHHHH
EFDELLTRIQNTLSLLSDSRPVYDYFLYPSNRAKAAVESSWVNLVKAEKFFSQLELIDNQ
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GIALFSLSYNAINNETSKHNLPLTVVNHSLLTTLSQLAAGQVFSEGVVLNEKLATSGVYK
CEEEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCEEECHHHHHHHHHH
PVMQIIIPIEVLGSRKGYVVAELSETRIENVIKFSPTLNISPSIVNQDGYYLLTNKRNKM
HHHHHHHHHHHHCCCCCEEEEECHHHHHHHHEEECCCCCCCCCEECCCCEEEEECCCCCH
FGHLVNGREDQTLKAENSELWRLMTTQKTGSAYIDKNIFSFYKFSLNQKVPNKEVYFLLD
HHHHCCCCCCCEEECCCCHHEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
YSEESLNVLFQEAKSELIEETTIVLLLLSLIVLPCGVALTLWDKSNIESQLAKSALEGMT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCE
AVVITDKYQRIIKVNSAFSLISGCCASETIGKTTFDVLKSGVEHSEKRVQNWKQVEELGV
EEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
WQGEIEALNKDEKKLSLLVRIQVVKNKRKDIQYYILSYIDITQRKLLEKELRYLSEKDSL
CCHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
SDCWNRRKFEFELENQTLLTNRYYPVHQCCLAILDIDWFKRINDTKGHDVGDKVIFLVAG
HHHHCCCEEEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
ILQKGSRDGGEEFAIIMPHTELKDAERVVNRLRVAVSQLEEYQLTISGGLADIGIAGNDA
HHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEECCCCC
YKCADLALYESKTNGRNLISICSDLGDLA
HHHHHEEEEECCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
SSTDMNKKSFKIVAYSSCVYLVLSMITLVYFYSEYQKEKLVIIEQVQNESTRQLAYTHH
CCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHCCCCHHHHHHHHH
EFDELLTRIQNTLSLLSDSRPVYDYFLYPSNRAKAAVESSWVNLVKAEKFFSQLELIDNQ
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GIALFSLSYNAINNETSKHNLPLTVVNHSLLTTLSQLAAGQVFSEGVVLNEKLATSGVYK
CEEEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCEEECHHHHHHHHHH
PVMQIIIPIEVLGSRKGYVVAELSETRIENVIKFSPTLNISPSIVNQDGYYLLTNKRNKM
HHHHHHHHHHHHCCCCCEEEEECHHHHHHHHEEECCCCCCCCCEECCCCEEEEECCCCCH
FGHLVNGREDQTLKAENSELWRLMTTQKTGSAYIDKNIFSFYKFSLNQKVPNKEVYFLLD
HHHHCCCCCCCEEECCCCHHEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
YSEESLNVLFQEAKSELIEETTIVLLLLSLIVLPCGVALTLWDKSNIESQLAKSALEGMT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCE
AVVITDKYQRIIKVNSAFSLISGCCASETIGKTTFDVLKSGVEHSEKRVQNWKQVEELGV
EEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
WQGEIEALNKDEKKLSLLVRIQVVKNKRKDIQYYILSYIDITQRKLLEKELRYLSEKDSL
CCHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
SDCWNRRKFEFELENQTLLTNRYYPVHQCCLAILDIDWFKRINDTKGHDVGDKVIFLVAG
HHHHCCCEEEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
ILQKGSRDGGEEFAIIMPHTELKDAERVVNRLRVAVSQLEEYQLTISGGLADIGIAGNDA
HHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEECCCCC
YKCADLALYESKTNGRNLISICSDLGDLA
HHHHHEEEEECCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]