Click here to switch to the map view.

The map label for this gene is dnaG [H]

Identifier: 209696104

GI number: 209696104

Start: 2917267

End: 2919027

Strand: Direct

Name: dnaG [H]

Synonym: VSAL_I2696

Alternate gene names: 209696104

Gene position: 2917267-2919027 (Clockwise)

Preceding gene: 209696103

Following gene: 209696105

Centisome position: 87.73

GC content: 41.17

Gene sequence:

>1761_bases
ATGTCCGGACATATCCCTCGTAGTTTTATTGATGAACTTTTAAATCGACACGATATTGTCGACATCATCGACACTAGAGT
AAAACTAAAAAAACAAGGGAAAAACTACGGTGCTTGTTGCCCTTTTCATAATGAAAAAAGTCCTTCTTTCTCTGTAAGCC
AAGAAAAGCAATTCTATCACTGCTTTGGCTGTGGCGTTCATGGTAATGCCATTGATTTCTTAATGGAATTCGACCGTCTT
GAGTTTGTAGAAGCCATCGAAGAACTTGCTTCAATGCTTGGTTTAGATGTTCCTCGCGAACAGCGTAGTGGCTTTAGTGC
GCCTTCTGGCCCAACCGCAAATTCAGAACAAAAACGCAGTTTATACGATCTAATGGGCGGTATTAGTCAGTTTTATCGTT
CTCAATTAAAATCCGCTAATAACATTGTTGCAATTAACTACCTTAAAGAACGCGGTCTCTCTAGTGAGATTGCTCAAAAA
TTTGGGGTTGGTTATATTGCTGATGAATGGGAATTAGTGAAAAATAACTTCGGTCGCGACAAAGCATCACAAGATGCTTT
AGTGAGTGGCGGGATGTTAATTGAAAACGATAAAGGGCGACGCTATGATCGCTTTCGTGGTCGAGTCATGTTTCCTATTC
GAGATCGTCGCGGACGCGTTATTGGTTTTGGTGGTCGAGTTATTGGTGAAGGTACACCGAAATACTTAAATTCTCCAGAA
ACGCCTATTTTCCATAAAGGCAAAGAACTTTATGGCTTATACGAAGCATTACAAGCGTATAAAGACCTGCCTCAAGCTTT
GGTTGTTGAAGGCTATATGGATGTTGTCGCCCTTGCTCAATTCGGTGTAGATTATGCCGTTGCCTCCCTAGGCACAGCAA
CTACCGGCGATCATATGCAATTACTTTTCCGTCAAACAAGCACTGTCGTATGTTGTTACGATGGAGACAGAGCGGGTCGG
GAAGCGGCATGGCGAGCAATGGAACAAGCGCTTCCCTACCTCAATGATGGCCGCCAATTAAAATTCATGTTTTTACCCGA
CGGTGAAGATCCAGATACCTATATCCGTCAATATGGTAAAGAAGCCTTCGAGCGAGAAATCGCTCAATCTGAATCATTGA
TTAATTTCATGTTTAGAAGCTTGATTGATCAAGTAGATACAAGCAGCCGAGAAGGCAAAGCAAAACTCACAACACTTGCT
GTTCCTCTCATAGACAAAGTTCCTGGTGGTACGTTACGCCTTTATTTACGTGAGCAACTTGGCAAAACGCTTGGAATTAT
GGATGAAAGCCAATTACAGCAACTAATATCTAAACAAGGTGCTCCTGAAACCAAGCGACAGGTACAACCAGATATTAAAC
GAACAGCGATGCGCGAAGTCATTACGTTGTTATTACAAAATCCAAATTTAGCTCAATTAGTCCCAGATTTAAGCAGTGTT
CAAGATATTCCACTGCCGGGTTTAAGTTTATTTCTACAATTACTTGAAAATTGCCGACATAATCCCCATATCACTACAGG
CCAATTATTAGAATATTGGCGCGGTGATAAAAATGAAAAACTATTATCACGCTTGGCAGCATGGGAACTCCATATAGACC
AAGACAATATACAAGATGTATTTATGGATTCGCTGGACAACATTTTAGCCCAGTGCATCAACCAACAAATTGAAAGTTTG
CAGACGAAAGAAAGAACGCTTGGCTTATCAGTCGAAGAAAAACGGGAGCTACTAGCCTTAATGCTAGAATTAAAAGCATA
G

Upstream 100 bases:

>100_bases
ATAAAGCAAGCCGTGCTATCTTTGATATGCACGGCTTGTTTGTATCTGAGATAATATAAACACTAGAATAGTATGAAAAA
CCATTGAGGTAAGAAGTAGC

Downstream 100 bases:

>100_bases
AATCCCGACATCGTCATCAATAAAAAAATTTGTTATTATTTGTGGTTTGCATTTCGCATACTAATTCTGTCACACAGACC
AAAAGTAGGAACCGTCTATG

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 586; Mature: 585

Protein sequence:

>586_residues
MSGHIPRSFIDELLNRHDIVDIIDTRVKLKKQGKNYGACCPFHNEKSPSFSVSQEKQFYHCFGCGVHGNAIDFLMEFDRL
EFVEAIEELASMLGLDVPREQRSGFSAPSGPTANSEQKRSLYDLMGGISQFYRSQLKSANNIVAINYLKERGLSSEIAQK
FGVGYIADEWELVKNNFGRDKASQDALVSGGMLIENDKGRRYDRFRGRVMFPIRDRRGRVIGFGGRVIGEGTPKYLNSPE
TPIFHKGKELYGLYEALQAYKDLPQALVVEGYMDVVALAQFGVDYAVASLGTATTGDHMQLLFRQTSTVVCCYDGDRAGR
EAAWRAMEQALPYLNDGRQLKFMFLPDGEDPDTYIRQYGKEAFEREIAQSESLINFMFRSLIDQVDTSSREGKAKLTTLA
VPLIDKVPGGTLRLYLREQLGKTLGIMDESQLQQLISKQGAPETKRQVQPDIKRTAMREVITLLLQNPNLAQLVPDLSSV
QDIPLPGLSLFLQLLENCRHNPHITTGQLLEYWRGDKNEKLLSRLAAWELHIDQDNIQDVFMDSLDNILAQCINQQIESL
QTKERTLGLSVEEKRELLALMLELKA

Sequences:

>Translated_586_residues
MSGHIPRSFIDELLNRHDIVDIIDTRVKLKKQGKNYGACCPFHNEKSPSFSVSQEKQFYHCFGCGVHGNAIDFLMEFDRL
EFVEAIEELASMLGLDVPREQRSGFSAPSGPTANSEQKRSLYDLMGGISQFYRSQLKSANNIVAINYLKERGLSSEIAQK
FGVGYIADEWELVKNNFGRDKASQDALVSGGMLIENDKGRRYDRFRGRVMFPIRDRRGRVIGFGGRVIGEGTPKYLNSPE
TPIFHKGKELYGLYEALQAYKDLPQALVVEGYMDVVALAQFGVDYAVASLGTATTGDHMQLLFRQTSTVVCCYDGDRAGR
EAAWRAMEQALPYLNDGRQLKFMFLPDGEDPDTYIRQYGKEAFEREIAQSESLINFMFRSLIDQVDTSSREGKAKLTTLA
VPLIDKVPGGTLRLYLREQLGKTLGIMDESQLQQLISKQGAPETKRQVQPDIKRTAMREVITLLLQNPNLAQLVPDLSSV
QDIPLPGLSLFLQLLENCRHNPHITTGQLLEYWRGDKNEKLLSRLAAWELHIDQDNIQDVFMDSLDNILAQCINQQIESL
QTKERTLGLSVEEKRELLALMLELKA
>Mature_585_residues
SGHIPRSFIDELLNRHDIVDIIDTRVKLKKQGKNYGACCPFHNEKSPSFSVSQEKQFYHCFGCGVHGNAIDFLMEFDRLE
FVEAIEELASMLGLDVPREQRSGFSAPSGPTANSEQKRSLYDLMGGISQFYRSQLKSANNIVAINYLKERGLSSEIAQKF
GVGYIADEWELVKNNFGRDKASQDALVSGGMLIENDKGRRYDRFRGRVMFPIRDRRGRVIGFGGRVIGEGTPKYLNSPET
PIFHKGKELYGLYEALQAYKDLPQALVVEGYMDVVALAQFGVDYAVASLGTATTGDHMQLLFRQTSTVVCCYDGDRAGRE
AAWRAMEQALPYLNDGRQLKFMFLPDGEDPDTYIRQYGKEAFEREIAQSESLINFMFRSLIDQVDTSSREGKAKLTTLAV
PLIDKVPGGTLRLYLREQLGKTLGIMDESQLQQLISKQGAPETKRQVQPDIKRTAMREVITLLLQNPNLAQLVPDLSSVQ
DIPLPGLSLFLQLLENCRHNPHITTGQLLEYWRGDKNEKLLSRLAAWELHIDQDNIQDVFMDSLDNILAQCINQQIESLQ
TKERTLGLSVEEKRELLALMLELKA

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=585, Percent_Identity=57.4358974358974, Blast_Score=694, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 66181; Mature: 66050

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGHIPRSFIDELLNRHDIVDIIDTRVKLKKQGKNYGACCPFHNEKSPSFSVSQEKQFYH
CCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCEEE
CFGCGVHGNAIDFLMEFDRLEFVEAIEELASMLGLDVPREQRSGFSAPSGPTANSEQKRS
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCHHHHH
LYDLMGGISQFYRSQLKSANNIVAINYLKERGLSSEIAQKFGVGYIADEWELVKNNFGRD
HHHHHHHHHHHHHHHHCCCCCEEEEEEHHHCCCHHHHHHHHCCCCCCHHHHHHHCCCCCC
KASQDALVSGGMLIENDKGRRYDRFRGRVMFPIRDRRGRVIGFGGRVIGEGTPKYLNSPE
CCCCCHHHCCCEEEECCCCCCHHHHCCEEEEEEECCCCCEEEECCEEECCCCCHHCCCCC
TPIFHKGKELYGLYEALQAYKDLPQALVVEGYMDVVALAQFGVDYAVASLGTATTGDHMQ
CCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LLFRQTSTVVCCYDGDRAGREAAWRAMEQALPYLNDGRQLKFMFLPDGEDPDTYIRQYGK
HHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHH
EAFEREIAQSESLINFMFRSLIDQVDTSSREGKAKLTTLAVPLIDKVPGGTLRLYLREQL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEEHHHHHCCCCCHHHHHHHHHH
GKTLGIMDESQLQQLISKQGAPETKRQVQPDIKRTAMREVITLLLQNPNLAQLVPDLSSV
HHHHCCCCHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHCCCHHHH
QDIPLPGLSLFLQLLENCRHNPHITTGQLLEYWRGDKNEKLLSRLAAWELHIDQDNIQDV
CCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHEECCHHHHHHH
FMDSLDNILAQCINQQIESLQTKERTLGLSVEEKRELLALMLELKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SGHIPRSFIDELLNRHDIVDIIDTRVKLKKQGKNYGACCPFHNEKSPSFSVSQEKQFYH
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCEEE
CFGCGVHGNAIDFLMEFDRLEFVEAIEELASMLGLDVPREQRSGFSAPSGPTANSEQKRS
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCHHHHH
LYDLMGGISQFYRSQLKSANNIVAINYLKERGLSSEIAQKFGVGYIADEWELVKNNFGRD
HHHHHHHHHHHHHHHHCCCCCEEEEEEHHHCCCHHHHHHHHCCCCCCHHHHHHHCCCCCC
KASQDALVSGGMLIENDKGRRYDRFRGRVMFPIRDRRGRVIGFGGRVIGEGTPKYLNSPE
CCCCCHHHCCCEEEECCCCCCHHHHCCEEEEEEECCCCCEEEECCEEECCCCCHHCCCCC
TPIFHKGKELYGLYEALQAYKDLPQALVVEGYMDVVALAQFGVDYAVASLGTATTGDHMQ
CCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LLFRQTSTVVCCYDGDRAGREAAWRAMEQALPYLNDGRQLKFMFLPDGEDPDTYIRQYGK
HHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHH
EAFEREIAQSESLINFMFRSLIDQVDTSSREGKAKLTTLAVPLIDKVPGGTLRLYLREQL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEEHHHHHCCCCCHHHHHHHHHH
GKTLGIMDESQLQQLISKQGAPETKRQVQPDIKRTAMREVITLLLQNPNLAQLVPDLSSV
HHHHCCCCHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHCCCHHHH
QDIPLPGLSLFLQLLENCRHNPHITTGQLLEYWRGDKNEKLLSRLAAWELHIDQDNIQDV
CCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHEECCHHHHHHH
FMDSLDNILAQCINQQIESLQTKERTLGLSVEEKRELLALMLELKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]