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The map label for this gene is pcnB [H]

Identifier: 209696020

GI number: 209696020

Start: 2823105

End: 2824430

Strand: Direct

Name: pcnB [H]

Synonym: VSAL_I2607

Alternate gene names: 209696020

Gene position: 2823105-2824430 (Clockwise)

Preceding gene: 209696019

Following gene: 209696021

Centisome position: 84.9

GC content: 40.2

Gene sequence:

>1326_bases
TTGAAACTGAACGTTATCACACGCCAAGAACATAATATTTCACGTAAAGACTTAAGTGATAATGCACTCAAAGTTCTTTA
CCGCCTAAGCAATGCTGGATACGACGCCTATCTAGTAGGAGGAGGTGTGCGCGATATAATTCTAGGACAAGAACCTAAAG
ATTTTGATATCGCCACCAATGCCACTCCCGAGCAAATCAAAAAACTATTTAGAAATTGCCGATTGATTGGCCGTAGATTC
CGTCTTGCTCATATTCTTTTTGGCAGAGATGTCATTGAGGTTGCCACTTTCCGTGGTCACCATAAAGAAGAAGAATCAGA
GAAAAAGAACATCTCTTCTCAATCAAAAGAAGGCATGTTATTGCGTGATAATGTCTATGGCACCGTTGAAGAAGATGCCC
AACGTCGTGACTTTACGATTAATGCGATGTATTACAACATTGCAGATTACTCAATTCATGATTATGCCAATGGCATTGAA
GATTTAAAAAATGGTGTCGTACGTCTTATCGGTGATCCTGAAACTCGTTATCGAGAAGATCCTGTTCGAATGCTACGTGC
GGTACGCTTTGCTGCAAAATTAGACATGACAATTTGCAATAAAACCGCAGAACCAATGGTTGAATTGTCCCCACTTTTAC
GTGATATCCCTGCGGCTCGTTTATTTGAAGAATCACTTAAACTACTTCAAGCAGGAAAAGGCTTAAATACCTATTTAATG
CTGCGTAAATTTAACTTGTTTCAACAGTTGTTTCCCGCGCTTACGCCATTTTTAACAAAAGAAGGCAACAGTCAAACGGA
ACAAATGATCGAACTTGTATTACATTCAACAGACAAACGTATAAATAGCGGTCAACGAGTTAACCCTGCATTCATGTTTG
CAGCAATGTTATGGTATCCATTAACGGCTCAAGCAAAAGAAATTCAAAACGAAAATAGCAAACTTGATGAATACGAATCC
ATTATGGAAGCGTCTAATATCATGCTTGATATTCAAGTAAAAAGCACGGCGGTCCCTCGTCGCTTAACCGCGACTATTCG
TGATATTTGGCAATTACAACTCCGTTTACCTCGTCGTACTGGTAAACGTGCGTTTATTCTTATTGACCAACAAAAATTCC
GTGCTGCTTATGATTTCCTTGAAATGCGTGGAAAAGTAGAAGGTGGTGATGTAAAAGCACTCGCTGAATGGTGGACTGAA
TTCCAGGACGCACCCGTTCCACACCGTAATAACATGATTCAAGGCTTACATGGTGGCCGTTCAACCGGTACACGTAAACG
TCGTAGCCCTTATCGTAAGAAAAAAAGAACGAATAACGATTCATGA

Upstream 100 bases:

>100_bases
ACTGCTCACTTGAATATCGAGGTAAATTATCTTTAATCAAGTCGCCCGTTTTTGTCGTAATTTACTCAAAAACAACAGTG
AAATCTGCTCAACAGAGGAT

Downstream 100 bases:

>100_bases
TCAAAGTAATTATCGCCATAGGGAGTAATCTAGGGGACCCTGTAGCCCAAGCAGAAGCGGCGATTGATGCTCTTCATCAA
TTACCAGAATCGACAGTGAC

Product: poly(A) polymerase (plasmid copy number protein)

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MKLNVITRQEHNISRKDLSDNALKVLYRLSNAGYDAYLVGGGVRDIILGQEPKDFDIATNATPEQIKKLFRNCRLIGRRF
RLAHILFGRDVIEVATFRGHHKEEESEKKNISSQSKEGMLLRDNVYGTVEEDAQRRDFTINAMYYNIADYSIHDYANGIE
DLKNGVVRLIGDPETRYREDPVRMLRAVRFAAKLDMTICNKTAEPMVELSPLLRDIPAARLFEESLKLLQAGKGLNTYLM
LRKFNLFQQLFPALTPFLTKEGNSQTEQMIELVLHSTDKRINSGQRVNPAFMFAAMLWYPLTAQAKEIQNENSKLDEYES
IMEASNIMLDIQVKSTAVPRRLTATIRDIWQLQLRLPRRTGKRAFILIDQQKFRAAYDFLEMRGKVEGGDVKALAEWWTE
FQDAPVPHRNNMIQGLHGGRSTGTRKRRSPYRKKKRTNNDS

Sequences:

>Translated_441_residues
MKLNVITRQEHNISRKDLSDNALKVLYRLSNAGYDAYLVGGGVRDIILGQEPKDFDIATNATPEQIKKLFRNCRLIGRRF
RLAHILFGRDVIEVATFRGHHKEEESEKKNISSQSKEGMLLRDNVYGTVEEDAQRRDFTINAMYYNIADYSIHDYANGIE
DLKNGVVRLIGDPETRYREDPVRMLRAVRFAAKLDMTICNKTAEPMVELSPLLRDIPAARLFEESLKLLQAGKGLNTYLM
LRKFNLFQQLFPALTPFLTKEGNSQTEQMIELVLHSTDKRINSGQRVNPAFMFAAMLWYPLTAQAKEIQNENSKLDEYES
IMEASNIMLDIQVKSTAVPRRLTATIRDIWQLQLRLPRRTGKRAFILIDQQKFRAAYDFLEMRGKVEGGDVKALAEWWTE
FQDAPVPHRNNMIQGLHGGRSTGTRKRRSPYRKKKRTNNDS
>Mature_441_residues
MKLNVITRQEHNISRKDLSDNALKVLYRLSNAGYDAYLVGGGVRDIILGQEPKDFDIATNATPEQIKKLFRNCRLIGRRF
RLAHILFGRDVIEVATFRGHHKEEESEKKNISSQSKEGMLLRDNVYGTVEEDAQRRDFTINAMYYNIADYSIHDYANGIE
DLKNGVVRLIGDPETRYREDPVRMLRAVRFAAKLDMTICNKTAEPMVELSPLLRDIPAARLFEESLKLLQAGKGLNTYLM
LRKFNLFQQLFPALTPFLTKEGNSQTEQMIELVLHSTDKRINSGQRVNPAFMFAAMLWYPLTAQAKEIQNENSKLDEYES
IMEASNIMLDIQVKSTAVPRRLTATIRDIWQLQLRLPRRTGKRAFILIDQQKFRAAYDFLEMRGKVEGGDVKALAEWWTE
FQDAPVPHRNNMIQGLHGGRSTGTRKRRSPYRKKKRTNNDS

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=222, Percent_Identity=32.4324324324324, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI87081691, Length=416, Percent_Identity=59.1346153846154, Blast_Score=521, Evalue=1e-149,
Organism=Escherichia coli, GI1789436, Length=246, Percent_Identity=28.8617886178862, Blast_Score=72, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6321016, Length=185, Percent_Identity=31.3513513513514, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 50875; Mature: 50875

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNVITRQEHNISRKDLSDNALKVLYRLSNAGYDAYLVGGGVRDIILGQEPKDFDIATN
CEEEEEECCCCCCCHHCCCHHHHHHHHHHHCCCCEEEEECCCHHEEEECCCCCCCCCCCC
ATPEQIKKLFRNCRLIGRRFRLAHILFGRDVIEVATFRGHHKEEESEKKNISSQSKEGML
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEE
LRDNVYGTVEEDAQRRDFTINAMYYNIADYSIHDYANGIEDLKNGVVRLIGDPETRYRED
EECCCCCCCHHHHHHCCEEEEEEEEEEECCCHHHHHHHHHHHHCCEEEEECCCHHHHCCC
PVRMLRAVRFAAKLDMTICNKTAEPMVELSPLLRDIPAARLFEESLKLLQAGKGLNTYLM
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHH
LRKFNLFQQLFPALTPFLTKEGNSQTEQMIELVLHSTDKRINSGQRVNPAFMFAAMLWYP
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHC
LTAQAKEIQNENSKLDEYESIMEASNIMLDIQVKSTAVPRRLTATIRDIWQLQLRLPRRT
CCHHHHHHHCCCCHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCC
GKRAFILIDQQKFRAAYDFLEMRGKVEGGDVKALAEWWTEFQDAPVPHRNNMIQGLHGGR
CCEEEEEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCC
STGTRKRRSPYRKKKRTNNDS
CCCCCHHCCHHHHHHCCCCCC
>Mature Secondary Structure
MKLNVITRQEHNISRKDLSDNALKVLYRLSNAGYDAYLVGGGVRDIILGQEPKDFDIATN
CEEEEEECCCCCCCHHCCCHHHHHHHHHHHCCCCEEEEECCCHHEEEECCCCCCCCCCCC
ATPEQIKKLFRNCRLIGRRFRLAHILFGRDVIEVATFRGHHKEEESEKKNISSQSKEGML
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEE
LRDNVYGTVEEDAQRRDFTINAMYYNIADYSIHDYANGIEDLKNGVVRLIGDPETRYRED
EECCCCCCCHHHHHHCCEEEEEEEEEEECCCHHHHHHHHHHHHCCEEEEECCCHHHHCCC
PVRMLRAVRFAAKLDMTICNKTAEPMVELSPLLRDIPAARLFEESLKLLQAGKGLNTYLM
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHH
LRKFNLFQQLFPALTPFLTKEGNSQTEQMIELVLHSTDKRINSGQRVNPAFMFAAMLWYP
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHC
LTAQAKEIQNENSKLDEYESIMEASNIMLDIQVKSTAVPRRLTATIRDIWQLQLRLPRRT
CCHHHHHHHCCCCHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCC
GKRAFILIDQQKFRAAYDFLEMRGKVEGGDVKALAEWWTEFQDAPVPHRNNMIQGLHGGR
CCEEEEEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCC
STGTRKRRSPYRKKKRTNNDS
CCCCCHHCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]