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The map label for this gene is acnB [H]

Identifier: 209696011

GI number: 209696011

Start: 2798754

End: 2801342

Strand: Reverse

Name: acnB [H]

Synonym: VSAL_I2595

Alternate gene names: 209696011

Gene position: 2801342-2798754 (Counterclockwise)

Preceding gene: 209696012

Following gene: 209696007

Centisome position: 84.25

GC content: 42.02

Gene sequence:

>2589_bases
GTGCTTGAAGCTTATCGTAAACACGTCGCAGAACGTGCTTCTGAAGGTGTGGTCCCAAGACCATTAAATGCTGAGCAAGT
AGCAGCATTAGTTGAACTAGTAAAAAATCCGCCACAAGGTGAAGAAGAACTCCTTCTTGATTTACTAGAAAATCGCATTC
CCCCAGGTGTTGATGAAGCAGCTTATGTTAAAGCCGGTTTTTTAACTGCAATTACAACGGGTGATATTTCTTCACCATTA
GTGAATCGTGAAAAAGCAGCAGAGCTACTAGGTACGATGCAAGGCGGCTACAATATTGATTCATTAGTAAGTTTACTTGA
TGACGTCGAATTAGCGCCTATCGCAGTTAAGGCATTATCCCATACATTATTAATGTTCGATTCTTTTTACGATGTAGAAG
AAAAAGCGAAGGCTGGCAATGTCTCAGCTCAACAGGTTATTCAATCTTGGGCAGATGCTGATTGGTTTACAGCAAAAGAA
AAAGTAGCTGAAAAAATTACCGTTAAAGTATTTAAAGTCACGGGTGAAACTAATACCGATGATTTATCACCAGCACCAGA
TGCTTGGTCTCGTCCTGATATACCTGTACATGCGAAAGCGATGCTAAAAATGGAACGAGAGGGTATTCACCCAGACCAAG
AGGGTAGCATTGGTCCAATTAATCAAATAGAAGAAATGCAAAAAGATGGTATTCCACTGGCTTACGTTGGTGATGTTGTT
GGTACTGGTTCTTCACGTAAATCAGCAACAAACTCAGTACTTTGGTTTATGGGTGAAGATATCCCATTCGTACCAAATAA
GCGCACTGGTGGTGTTTGTTTAGGTGGTAAGATTGCGCCAATCTTCTACAACACAATGGAAGACTCAGGTGCACTACCGA
TTGAATTGAATGTTCAAGACATGAATATGGGTGATGTCATAGATATATTCCCTTATGAAGGTGTTGTTCGTAAAGATGAT
GCGGAAATTTCTAGTTTTAAGCTTGGAAAAGTATTGCTTGATGAAGTTCGTGCCGGTGGTCGTATTCCACTGATCATTGG
TCGTGGTTTAACAAGTCGTGCTCGTACTTCTCTTGGTCTTGAAGAGACAGATTTATTTGCTAAACCCGTTGATCCATCGG
TTTCAGATAAAGGCTACACTTTAGCTCAGAAGATGGTTGGTAAAGCTTGTGGAGTTGAAGGTGTTCGTGCTGGTCAGTAT
TGTGAACCTAAAATGACCACAGTGGGCTCTCAGGATACCACTGGCCCTATGACTCGTGATGAACTAAAAGATCTTGCTTG
TCTTGGCTTCTCTGCTGATCTTGTAATGCAGTCATTCTGTCATACATCGGCTTATCCAAAACCTGTTGATGTTAATACTC
ACCATACACTTCCTGATTTTATTATGAATCGTGGCGGAGTTTCACTTCGTCCGGGTGACGGTGTTATTCACTCTTGGCTA
AACCGTATGCTTCTACCTGATACCGTGGGTACGGGGGGGGATTCGCATACACGTTTCCCTCTAGGTATTTCTTTTCCTGC
CGGTTCTGGCCTTGTTGCCTTTGCTGCTGCGACAGGCGTTATGCCTCTAGATATGCCTGAATCAATTTTGGTTCGTTTTA
AAGGTGAGATGCAACCAGGTATTACACTGCGTGATCTGGTTCATGCAATCCCTTACTACGGTATTCAGCAAGGGTTACTA
ACAGTCGCTAAAGCGGGTAAAGTAAATGAATTCTCTGGTCGTGTACTTGAGATTGAAGGTGTTGAACATTTATCTGTAGA
ACAAGCGTTTGAGTTATCAGATGCATCGGCAGAACGTTCAGCGGCTGGTTGTACAGTTAAGCTTTCTCAAGGGTCAATTG
AGGAGTACTTGAATTCAAACATTGTTATGTTGAAGTGGATGATTTCTGAAGGTTATGGCGATCGCCGTACACTTGAGCGT
CGTGTGACTTCAATGGAAGAATGGTTAGCGAATCCAGAGCTAATGGAAGCAGATAAAGACGCGGAATATGCGCATGTAAT
AGATATTGATCTTGCCGATATTAAAGAACCAATTCTTTGTGCTCCAAATGATCCTGATGATGCTCGTTTACTTTCAGAAG
TTCAAGGTACAGAAATTGATGAAGTGTTCATTGGTTCTTGTATGACGAATATTGGTCATTTCCGTGCCGCAGGTAAAATG
CTAGAAGAGTTTAATGGCTCTCTAAGTACTCGTTTATGGGTTGCTCCACCGACGAAAATGGATAAAGATCAACTAATAGA
AGAAGGCTACTACGGGATCTTTGGTCGTGCTGGGGTTCGTATTGAAACTCCGGGTTGTTCTCTATGTATGGGTAACCAAG
CCCGTGTTGCTGATAAAGCAACAGTAATGTCGACATCAACACGTAACTTCCCGAACCGTTTAGGTAATGGTGCTAATGTT
TATCTAGCGTCTGCTGAGTTATCTGCGGTTGGAGCGATTCTAGGTCGAATTCCAACGCAAGCTGAGTATTTAGAATATGC
TCAAAAAGTGAATGCAACAGCAGCGGATACGTATCGTTACTTGAATTTCCATAAAATGGGTCAATACACAGACAAAGCAG
ATACGGTTATTTTTCAAGAACCTGCTTAA

Upstream 100 bases:

>100_bases
TGAGATTTATAACAGTATTTAAAAGTTAATGGATATAAACTGAATCGTTTCCAAGGATGTTTACTCATTAAGAGTAGACC
AGAAAGTGAGGAATTAAGTC

Downstream 100 bases:

>100_bases
TCTTTGTTTATAAATAAAAAAGCAGCCAATTGGCTGCTTTTTTATTGCGTATTATTTGATTCCACAAGGGTATATTTGGA
GGTACTACATTATTTTTTTG

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase; 2-methylisocitrate dehydratase; Citrate hydro-lyase [H]

Number of amino acids: Translated: 862; Mature: 862

Protein sequence:

>862_residues
MLEAYRKHVAERASEGVVPRPLNAEQVAALVELVKNPPQGEEELLLDLLENRIPPGVDEAAYVKAGFLTAITTGDISSPL
VNREKAAELLGTMQGGYNIDSLVSLLDDVELAPIAVKALSHTLLMFDSFYDVEEKAKAGNVSAQQVIQSWADADWFTAKE
KVAEKITVKVFKVTGETNTDDLSPAPDAWSRPDIPVHAKAMLKMEREGIHPDQEGSIGPINQIEEMQKDGIPLAYVGDVV
GTGSSRKSATNSVLWFMGEDIPFVPNKRTGGVCLGGKIAPIFYNTMEDSGALPIELNVQDMNMGDVIDIFPYEGVVRKDD
AEISSFKLGKVLLDEVRAGGRIPLIIGRGLTSRARTSLGLEETDLFAKPVDPSVSDKGYTLAQKMVGKACGVEGVRAGQY
CEPKMTTVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTSAYPKPVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWL
NRMLLPDTVGTGGDSHTRFPLGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGEMQPGITLRDLVHAIPYYGIQQGLL
TVAKAGKVNEFSGRVLEIEGVEHLSVEQAFELSDASAERSAAGCTVKLSQGSIEEYLNSNIVMLKWMISEGYGDRRTLER
RVTSMEEWLANPELMEADKDAEYAHVIDIDLADIKEPILCAPNDPDDARLLSEVQGTEIDEVFIGSCMTNIGHFRAAGKM
LEEFNGSLSTRLWVAPPTKMDKDQLIEEGYYGIFGRAGVRIETPGCSLCMGNQARVADKATVMSTSTRNFPNRLGNGANV
YLASAELSAVGAILGRIPTQAEYLEYAQKVNATAADTYRYLNFHKMGQYTDKADTVIFQEPA

Sequences:

>Translated_862_residues
MLEAYRKHVAERASEGVVPRPLNAEQVAALVELVKNPPQGEEELLLDLLENRIPPGVDEAAYVKAGFLTAITTGDISSPL
VNREKAAELLGTMQGGYNIDSLVSLLDDVELAPIAVKALSHTLLMFDSFYDVEEKAKAGNVSAQQVIQSWADADWFTAKE
KVAEKITVKVFKVTGETNTDDLSPAPDAWSRPDIPVHAKAMLKMEREGIHPDQEGSIGPINQIEEMQKDGIPLAYVGDVV
GTGSSRKSATNSVLWFMGEDIPFVPNKRTGGVCLGGKIAPIFYNTMEDSGALPIELNVQDMNMGDVIDIFPYEGVVRKDD
AEISSFKLGKVLLDEVRAGGRIPLIIGRGLTSRARTSLGLEETDLFAKPVDPSVSDKGYTLAQKMVGKACGVEGVRAGQY
CEPKMTTVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTSAYPKPVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWL
NRMLLPDTVGTGGDSHTRFPLGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGEMQPGITLRDLVHAIPYYGIQQGLL
TVAKAGKVNEFSGRVLEIEGVEHLSVEQAFELSDASAERSAAGCTVKLSQGSIEEYLNSNIVMLKWMISEGYGDRRTLER
RVTSMEEWLANPELMEADKDAEYAHVIDIDLADIKEPILCAPNDPDDARLLSEVQGTEIDEVFIGSCMTNIGHFRAAGKM
LEEFNGSLSTRLWVAPPTKMDKDQLIEEGYYGIFGRAGVRIETPGCSLCMGNQARVADKATVMSTSTRNFPNRLGNGANV
YLASAELSAVGAILGRIPTQAEYLEYAQKVNATAADTYRYLNFHKMGQYTDKADTVIFQEPA
>Mature_862_residues
MLEAYRKHVAERASEGVVPRPLNAEQVAALVELVKNPPQGEEELLLDLLENRIPPGVDEAAYVKAGFLTAITTGDISSPL
VNREKAAELLGTMQGGYNIDSLVSLLDDVELAPIAVKALSHTLLMFDSFYDVEEKAKAGNVSAQQVIQSWADADWFTAKE
KVAEKITVKVFKVTGETNTDDLSPAPDAWSRPDIPVHAKAMLKMEREGIHPDQEGSIGPINQIEEMQKDGIPLAYVGDVV
GTGSSRKSATNSVLWFMGEDIPFVPNKRTGGVCLGGKIAPIFYNTMEDSGALPIELNVQDMNMGDVIDIFPYEGVVRKDD
AEISSFKLGKVLLDEVRAGGRIPLIIGRGLTSRARTSLGLEETDLFAKPVDPSVSDKGYTLAQKMVGKACGVEGVRAGQY
CEPKMTTVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTSAYPKPVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWL
NRMLLPDTVGTGGDSHTRFPLGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGEMQPGITLRDLVHAIPYYGIQQGLL
TVAKAGKVNEFSGRVLEIEGVEHLSVEQAFELSDASAERSAAGCTVKLSQGSIEEYLNSNIVMLKWMISEGYGDRRTLER
RVTSMEEWLANPELMEADKDAEYAHVIDIDLADIKEPILCAPNDPDDARLLSEVQGTEIDEVFIGSCMTNIGHFRAAGKM
LEEFNGSLSTRLWVAPPTKMDKDQLIEEGYYGIFGRAGVRIETPGCSLCMGNQARVADKATVMSTSTRNFPNRLGNGANV
YLASAELSAVGAILGRIPTQAEYLEYAQKVNATAADTYRYLNFHKMGQYTDKADTVIFQEPA

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=862, Percent_Identity=77.7262180974478, Blast_Score=1373, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=372, Percent_Identity=26.3440860215054, Blast_Score=73, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6323335, Length=380, Percent_Identity=25.7894736842105, Blast_Score=71, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 93588; Mature: 93588

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEAYRKHVAERASEGVVPRPLNAEQVAALVELVKNPPQGEEELLLDLLENRIPPGVDEA
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCH
AYVKAGFLTAITTGDISSPLVNREKAAELLGTMQGGYNIDSLVSLLDDVELAPIAVKALS
HHHHHCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHH
HTLLMFDSFYDVEEKAKAGNVSAQQVIQSWADADWFTAKEKVAEKITVKVFKVTGETNTD
HHHHHHHHHCCHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHEEEEEEEEEECCCCCC
DLSPAPDAWSRPDIPVHAKAMLKMEREGIHPDQEGSIGPINQIEEMQKDGIPLAYVGDVV
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCEEEECHHH
GTGSSRKSATNSVLWFMGEDIPFVPNKRTGGVCLGGKIAPIFYNTMEDSGALPIELNVQD
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCEEEEEEEEECCCCCCCEEEEEEEE
MNMGDVIDIFPYEGVVRKDDAEISSFKLGKVLLDEVRAGGRIPLIIGRGLTSRARTSLGL
CCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHCCC
EETDLFAKPVDPSVSDKGYTLAQKMVGKACGVEGVRAGQYCEPKMTTVGSQDTTGPMTRD
CHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
ELKDLACLGFSADLVMQSFCHTSAYPKPVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWL
HHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCEECCCCCHHHHHH
NRMLLPDTVGTGGDSHTRFPLGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGEMQPG
HHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHHEECCCCCCC
ITLRDLVHAIPYYGIQQGLLTVAKAGKVNEFSGRVLEIEGVEHLSVEQAFELSDASAERS
CCHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHCCCCCHHHC
AAGCTVKLSQGSIEEYLNSNIVMLKWMISEGYGDRRTLERRVTSMEEWLANPELMEADKD
CCCCEEEECCCCHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHCCCCC
AEYAHVIDIDLADIKEPILCAPNDPDDARLLSEVQGTEIDEVFIGSCMTNIGHFRAAGKM
CCEEEEEEEEHHHCCCCEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LEEFNGSLSTRLWVAPPTKMDKDQLIEEGYYGIFGRAGVRIETPGCSLCMGNQARVADKA
HHHHCCCCCEEEEECCCCCCCHHHHHHCCCCEEECCCCEEEECCCCCEEECCCCCCCCHH
TVMSTSTRNFPNRLGNGANVYLASAELSAVGAILGRIPTQAEYLEYAQKVNATAADTYRY
HEEECCCCCCHHHCCCCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHH
LNFHKMGQYTDKADTVIFQEPA
HHHHHCCCCCCCCCEEEEECCC
>Mature Secondary Structure
MLEAYRKHVAERASEGVVPRPLNAEQVAALVELVKNPPQGEEELLLDLLENRIPPGVDEA
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCH
AYVKAGFLTAITTGDISSPLVNREKAAELLGTMQGGYNIDSLVSLLDDVELAPIAVKALS
HHHHHCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHH
HTLLMFDSFYDVEEKAKAGNVSAQQVIQSWADADWFTAKEKVAEKITVKVFKVTGETNTD
HHHHHHHHHCCHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHEEEEEEEEEECCCCCC
DLSPAPDAWSRPDIPVHAKAMLKMEREGIHPDQEGSIGPINQIEEMQKDGIPLAYVGDVV
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCEEEECHHH
GTGSSRKSATNSVLWFMGEDIPFVPNKRTGGVCLGGKIAPIFYNTMEDSGALPIELNVQD
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCEEEEEEEEECCCCCCCEEEEEEEE
MNMGDVIDIFPYEGVVRKDDAEISSFKLGKVLLDEVRAGGRIPLIIGRGLTSRARTSLGL
CCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHCCC
EETDLFAKPVDPSVSDKGYTLAQKMVGKACGVEGVRAGQYCEPKMTTVGSQDTTGPMTRD
CHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
ELKDLACLGFSADLVMQSFCHTSAYPKPVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWL
HHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCEECCCCCHHHHHH
NRMLLPDTVGTGGDSHTRFPLGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGEMQPG
HHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHHEECCCCCCC
ITLRDLVHAIPYYGIQQGLLTVAKAGKVNEFSGRVLEIEGVEHLSVEQAFELSDASAERS
CCHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHCCCCCHHHC
AAGCTVKLSQGSIEEYLNSNIVMLKWMISEGYGDRRTLERRVTSMEEWLANPELMEADKD
CCCCEEEECCCCHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHCCCCC
AEYAHVIDIDLADIKEPILCAPNDPDDARLLSEVQGTEIDEVFIGSCMTNIGHFRAAGKM
CCEEEEEEEEHHHCCCCEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LEEFNGSLSTRLWVAPPTKMDKDQLIEEGYYGIFGRAGVRIETPGCSLCMGNQARVADKA
HHHHCCCCCEEEEECCCCCCCHHHHHHCCCCEEECCCCEEEECCCCCEEECCCCCCCCHH
TVMSTSTRNFPNRLGNGANVYLASAELSAVGAILGRIPTQAEYLEYAQKVNATAADTYRY
HEEECCCCCCHHHCCCCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHH
LNFHKMGQYTDKADTVIFQEPA
HHHHHCCCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 8932712; 9298646; 10585860; 8000525; 11992126 [H]