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The map label for this gene is amtB [C]

Identifier: 209696005

GI number: 209696005

Start: 2792706

End: 2793935

Strand: Reverse

Name: amtB [C]

Synonym: VSAL_I2589

Alternate gene names: 209696005

Gene position: 2793935-2792706 (Counterclockwise)

Preceding gene: 209696006

Following gene: 209696004

Centisome position: 84.02

GC content: 40.57

Gene sequence:

>1230_bases
ATGGAACTTTCAATTACAGTATCAGAACTTCGTTATGCCCTTGATACCTTCTTCTTTTTAATGTCTGGCGCTCTTGTTAT
GTGGATGGCCGCCGGCTTTGCAATGTTAGAGGCTGGTCTGGTTCGCTCAAAAAATACCACTGAAATTTTAACTAAAAATC
TTTGTTTGTATGCGATAGCTTGTACAACATACTTAATTGTCGGTTATAACATTATGTATGTTGATAATGTGAGTGGTGGT
TTTCTACCTTCATTCGGAACATTAATTGGAACTCAAGTAGAAGGTGCAGATCACTCATTAGAGTCTGATTTTTTCTTTCA
AGTTGTATTTGTTGCGACGGCAATGTCAGTTGTTTCTGGTGCCGTGGCTGAGCGAATGAAGCTATGGTCTTTTTTAGTAT
TCTCTGTGATTTTAACCGCGTTTATTTATCCGATGGAAGGGTATTGGACATGGGGGGGTGGTTTCTTATCAGAAGCTGGG
TTTAGTGATTTTGCTGGTTCAGGTATTGTTCATATGGCAGGGGCTTCAGCGGCATTAGCCGGAGTATTATTGCTCGGTGC
ACGTAAAGGTAAATACGGTAAGAAAGGTCAAATCTATCCAATTCCAGGATCTAATATGCCATTAGCAACATTAGGTACTT
TTATTTTATGGTTTGGTTGGTTTGGTTTTAATGGCGGCTCTCAATTGATGATTTCTGATTTTGAAAATGCGACAGCAGTC
GGCCAAATCTTCTTAAATACGAATGCAGCCGCAGCTGCAGGGGCGATAGCTGCGTTATTTGTGTGTAAAATAACGTGGGG
AAAAGCCGATTTAACAATGGTATTAAATGGGGCGTTAGCAGGTCTTGTTGCTATCACAGCAGATCCGTTATCACCTTCTC
CTTTAGCTGCTGTTATTGTGGGTGCAGTTGCTGGAGCGTTAGTTATTTTTAGTATTGTGGCCTTTGATAAAATTAAAATT
GATGATCCAGTAGGGGCGATTTCAGTGCATGGTGTTTGTGGTTTTTTTGGGTTAATGGTTGTGCCACTGAGTAATGCGGA
TGCTAGTTTTGGTGCGCAGTTATTGGGAGCGGTTGTTATCTTTGCTTGGGTATTTATAGCAAGCCTTGTAGTTTGGGCAA
TAATTAAAGCAACCATCGGAATTCGAGTTACAGAGGAAGAAGAACTAGAAGGGATGGATGTTCATGATTGTGGTATTGAT
GCTTATCCTGAGTTTGTCAGTGTGAAATAA

Upstream 100 bases:

>100_bases
GTGATGGTAAAATTTTTGTTTATGACCTTCAGCAAGCGGTACGTATTCGTACTGGTGAAACTGACACTGAAGCACTTTAA
GGATAGAGGACAATAATATT

Downstream 100 bases:

>100_bases
ACAGCATTGCTAATATGTTGTTACATAATTATTAACCTCAATGCGGTCGTATTGAGGTTTTTCTTCATAAAAGATGAGTC
TGGTCATTGTCATTTTTAAA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MELSITVSELRYALDTFFFLMSGALVMWMAAGFAMLEAGLVRSKNTTEILTKNLCLYAIACTTYLIVGYNIMYVDNVSGG
FLPSFGTLIGTQVEGADHSLESDFFFQVVFVATAMSVVSGAVAERMKLWSFLVFSVILTAFIYPMEGYWTWGGGFLSEAG
FSDFAGSGIVHMAGASAALAGVLLLGARKGKYGKKGQIYPIPGSNMPLATLGTFILWFGWFGFNGGSQLMISDFENATAV
GQIFLNTNAAAAAGAIAALFVCKITWGKADLTMVLNGALAGLVAITADPLSPSPLAAVIVGAVAGALVIFSIVAFDKIKI
DDPVGAISVHGVCGFFGLMVVPLSNADASFGAQLLGAVVIFAWVFIASLVVWAIIKATIGIRVTEEEELEGMDVHDCGID
AYPEFVSVK

Sequences:

>Translated_409_residues
MELSITVSELRYALDTFFFLMSGALVMWMAAGFAMLEAGLVRSKNTTEILTKNLCLYAIACTTYLIVGYNIMYVDNVSGG
FLPSFGTLIGTQVEGADHSLESDFFFQVVFVATAMSVVSGAVAERMKLWSFLVFSVILTAFIYPMEGYWTWGGGFLSEAG
FSDFAGSGIVHMAGASAALAGVLLLGARKGKYGKKGQIYPIPGSNMPLATLGTFILWFGWFGFNGGSQLMISDFENATAV
GQIFLNTNAAAAAGAIAALFVCKITWGKADLTMVLNGALAGLVAITADPLSPSPLAAVIVGAVAGALVIFSIVAFDKIKI
DDPVGAISVHGVCGFFGLMVVPLSNADASFGAQLLGAVVIFAWVFIASLVVWAIIKATIGIRVTEEEELEGMDVHDCGID
AYPEFVSVK
>Mature_409_residues
MELSITVSELRYALDTFFFLMSGALVMWMAAGFAMLEAGLVRSKNTTEILTKNLCLYAIACTTYLIVGYNIMYVDNVSGG
FLPSFGTLIGTQVEGADHSLESDFFFQVVFVATAMSVVSGAVAERMKLWSFLVFSVILTAFIYPMEGYWTWGGGFLSEAG
FSDFAGSGIVHMAGASAALAGVLLLGARKGKYGKKGQIYPIPGSNMPLATLGTFILWFGWFGFNGGSQLMISDFENATAV
GQIFLNTNAAAAAGAIAALFVCKITWGKADLTMVLNGALAGLVAITADPLSPSPLAAVIVGAVAGALVIFSIVAFDKIKI
DDPVGAISVHGVCGFFGLMVVPLSNADASFGAQLLGAVVIFAWVFIASLVVWAIIKATIGIRVTEEEELEGMDVHDCGID
AYPEFVSVK

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=409, Percent_Identity=30.3178484107579, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17550180, Length=423, Percent_Identity=35.6973995271868, Blast_Score=201, Evalue=5e-52,
Organism=Caenorhabditis elegans, GI17550182, Length=426, Percent_Identity=32.6291079812207, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI32564042, Length=428, Percent_Identity=27.803738317757, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI133931100, Length=344, Percent_Identity=29.6511627906977, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6325396, Length=421, Percent_Identity=28.5035629453682, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324187, Length=412, Percent_Identity=28.1553398058252, Blast_Score=116, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6321559, Length=421, Percent_Identity=28.0285035629454, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI161078314, Length=411, Percent_Identity=31.8734793187348, Blast_Score=174, Evalue=7e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 43132; Mature: 43132

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSITVSELRYALDTFFFLMSGALVMWMAAGFAMLEAGLVRSKNTTEILTKNLCLYAIA
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
CTTYLIVGYNIMYVDNVSGGFLPSFGTLIGTQVEGADHSLESDFFFQVVFVATAMSVVSG
HHHHHHHCCEEEEEECCCCCCCCCHHHHHCCEECCCCCCCCHHHHHHHHHHHHHHHHHHH
AVAERMKLWSFLVFSVILTAFIYPMEGYWTWGGGFLSEAGFSDFAGSGIVHMAGASAALA
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHCCCCCCCCCCCEEEECCHHHHHH
GVLLLGARKGKYGKKGQIYPIPGSNMPLATLGTFILWFGWFGFNGGSQLMISDFENATAV
HHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEE
GQIFLNTNAAAAAGAIAALFVCKITWGKADLTMVLNGALAGLVAITADPLSPSPLAAVIV
EEEEEECCCHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHH
GAVAGALVIFSIVAFDKIKIDDPVGAISVHGVCGFFGLMVVPLSNADASFGAQLLGAVVI
HHHHHHHHHHHHHHHHEEEECCCCCCEEHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHH
FAWVFIASLVVWAIIKATIGIRVTEEEELEGMDVHDCGIDAYPEFVSVK
HHHHHHHHHHHHHHHHHHHCEEECCHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MELSITVSELRYALDTFFFLMSGALVMWMAAGFAMLEAGLVRSKNTTEILTKNLCLYAIA
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
CTTYLIVGYNIMYVDNVSGGFLPSFGTLIGTQVEGADHSLESDFFFQVVFVATAMSVVSG
HHHHHHHCCEEEEEECCCCCCCCCHHHHHCCEECCCCCCCCHHHHHHHHHHHHHHHHHHH
AVAERMKLWSFLVFSVILTAFIYPMEGYWTWGGGFLSEAGFSDFAGSGIVHMAGASAALA
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHCCCCCCCCCCCEEEECCHHHHHH
GVLLLGARKGKYGKKGQIYPIPGSNMPLATLGTFILWFGWFGFNGGSQLMISDFENATAV
HHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEE
GQIFLNTNAAAAAGAIAALFVCKITWGKADLTMVLNGALAGLVAITADPLSPSPLAAVIV
EEEEEECCCHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHH
GAVAGALVIFSIVAFDKIKIDDPVGAISVHGVCGFFGLMVVPLSNADASFGAQLLGAVVI
HHHHHHHHHHHHHHHHEEEECCCCCCEEHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHH
FAWVFIASLVVWAIIKATIGIRVTEEEELEGMDVHDCGIDAYPEFVSVK
HHHHHHHHHHHHHHHHHHHCEEECCHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]