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The map label for this gene is gltD [H]

Identifier: 209695980

GI number: 209695980

Start: 2749866

End: 2751338

Strand: Direct

Name: gltD [H]

Synonym: VSAL_I2563

Alternate gene names: 209695980

Gene position: 2749866-2751338 (Clockwise)

Preceding gene: 209695979

Following gene: 209695981

Centisome position: 82.7

GC content: 41.28

Gene sequence:

>1473_bases
ATGGGCAAGCCTACAGGTTTTTTAGAAAATGGTCGTGAGTTACCAAAAAAATTAGAACCAATTGTTCGAATTCAAGATAA
TAAAGAATTTGTCTTAAACGAAGAATTTGGCGATAAAATCAATACGCAAGCATCTCGCTGTATGGATTGTGGTGTTCCGT
TTTGTCATAACGGATGCCCTATTGGGAACATCATTCCGGAATTTAACGATGCCGTATATCGCGATAGCTGGGAAGAAGCT
TGGCATATTCTAAGCTCAACAAATAACTTTCCTGAATTTACAGGCCGTGTATGCCCTGCTCCTTGTGAAAGTGCATGTGT
ATTAGGGATCAATCAAGATCCAATCACAATCTGTAATATTGAAAAAACAATCGTTGAGACTGCGTATCGTGAAGGATACG
CACAACCTAAAAAGCCTCGTTCCCGTACAGGAAAAACTGTCGCGATTATTGGTTCAGGTCCTGCCGGCTTAGCTGCTGCA
GAGCAAATCAATAGTGCAGGCCATTCAGTCACTGTTTTTGAACGAGATGAGAAAGTTGGTGGCTTATTACGTTTCGGTAT
TCCTGATTTCAAACTAGGAATGGATATCATTGATCGAAAGATCAATTTAATGGCCGAAGCCGGTATTAAGTTTGAAGTAA
ATGCACATATTGGCGTTTCAATTAATGCTCAACAACTGCGCCAAGATTTTGATGTTGTATTACTGACTGGTGGTTCAACA
GTACCAAGGGATCTTCCTATTCCTGGTCGTGAATTAAATGGGGTTCATTTTGCAATGGAATTCCTAGGTCAAAACAATCG
CCGAGCGAACAATATGGATCTTAAAACAAAAGAGATCCATGCAAAAGATAAACATGTTGTCGTTATCGGTGGTGGCGATA
CAGGATCAGATTGCGTAGGTACTTCTAACCGTCATGGTGCAGCAAGCATTACTCAAGTTGAGATAATGCCTATTCCACCA
GAAAAGCGCCCAGCGAATATGCCTTGGCCTCAATACCCAATGATCATGAAAACAACGACTTCCCACGAAGAAGGCTGTGA
GCGTCATTGGAACATTTTAACTAAAGAGTTTATCTCTGATGATGAGGGTAATGTCACCGGCCTCCGTATTGCTGATATCG
TTTGGCAAGATGCCAAACCTGGAGAGCGACCTGGTTTTAACGAAGTAGCAAATAGTGAGCGAGTTATACCATGTGATCAA
GCTTTCTTAGCTATGGGATTTTTACACCCAGAACCTACGGGTGTTCTAGCTCAATTAGATATTAAACTTGATGATCGTGG
CAACGTTGCTACCAATGGTTTTTCTACTAACCAAAAAGGTGTTTTTGCTGCAGGTGATATGCGTACAGGGCAATCATTAA
TTGTACGTTGTATTAATGAAGGTCGTGAATGTGCGCGTGAAGTTGACGCTTATCTTATGGGGAATACAAACTTAGAAGCG
AAAGCAGGCTCTCTAATGCTATCAGCATCATAA

Upstream 100 bases:

>100_bases
CCTCTCTGGCTAAAGTTATGCCGCGAGACTATAAAGCGGTACTTCAAAAGCGTAAGACTGAAGCACAACACGCACAAATG
GAAGAAGTGGAGGCAGTATA

Downstream 100 bases:

>100_bases
TCTTTCGCAATTAAATTCAGGTTCCTTCCAAACCTTTGGCCAACATTTTGATGCTGGCCTTTTTTATAGTAAATTCAATA
AACAAAAATTCAACAATTCA

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MGKPTGFLENGRELPKKLEPIVRIQDNKEFVLNEEFGDKINTQASRCMDCGVPFCHNGCPIGNIIPEFNDAVYRDSWEEA
WHILSSTNNFPEFTGRVCPAPCESACVLGINQDPITICNIEKTIVETAYREGYAQPKKPRSRTGKTVAIIGSGPAGLAAA
EQINSAGHSVTVFERDEKVGGLLRFGIPDFKLGMDIIDRKINLMAEAGIKFEVNAHIGVSINAQQLRQDFDVVLLTGGST
VPRDLPIPGRELNGVHFAMEFLGQNNRRANNMDLKTKEIHAKDKHVVVIGGGDTGSDCVGTSNRHGAASITQVEIMPIPP
EKRPANMPWPQYPMIMKTTTSHEEGCERHWNILTKEFISDDEGNVTGLRIADIVWQDAKPGERPGFNEVANSERVIPCDQ
AFLAMGFLHPEPTGVLAQLDIKLDDRGNVATNGFSTNQKGVFAAGDMRTGQSLIVRCINEGRECAREVDAYLMGNTNLEA
KAGSLMLSAS

Sequences:

>Translated_490_residues
MGKPTGFLENGRELPKKLEPIVRIQDNKEFVLNEEFGDKINTQASRCMDCGVPFCHNGCPIGNIIPEFNDAVYRDSWEEA
WHILSSTNNFPEFTGRVCPAPCESACVLGINQDPITICNIEKTIVETAYREGYAQPKKPRSRTGKTVAIIGSGPAGLAAA
EQINSAGHSVTVFERDEKVGGLLRFGIPDFKLGMDIIDRKINLMAEAGIKFEVNAHIGVSINAQQLRQDFDVVLLTGGST
VPRDLPIPGRELNGVHFAMEFLGQNNRRANNMDLKTKEIHAKDKHVVVIGGGDTGSDCVGTSNRHGAASITQVEIMPIPP
EKRPANMPWPQYPMIMKTTTSHEEGCERHWNILTKEFISDDEGNVTGLRIADIVWQDAKPGERPGFNEVANSERVIPCDQ
AFLAMGFLHPEPTGVLAQLDIKLDDRGNVATNGFSTNQKGVFAAGDMRTGQSLIVRCINEGRECAREVDAYLMGNTNLEA
KAGSLMLSAS
>Mature_489_residues
GKPTGFLENGRELPKKLEPIVRIQDNKEFVLNEEFGDKINTQASRCMDCGVPFCHNGCPIGNIIPEFNDAVYRDSWEEAW
HILSSTNNFPEFTGRVCPAPCESACVLGINQDPITICNIEKTIVETAYREGYAQPKKPRSRTGKTVAIIGSGPAGLAAAE
QINSAGHSVTVFERDEKVGGLLRFGIPDFKLGMDIIDRKINLMAEAGIKFEVNAHIGVSINAQQLRQDFDVVLLTGGSTV
PRDLPIPGRELNGVHFAMEFLGQNNRRANNMDLKTKEIHAKDKHVVVIGGGDTGSDCVGTSNRHGAASITQVEIMPIPPE
KRPANMPWPQYPMIMKTTTSHEEGCERHWNILTKEFISDDEGNVTGLRIADIVWQDAKPGERPGFNEVANSERVIPCDQA
FLAMGFLHPEPTGVLAQLDIKLDDRGNVATNGFSTNQKGVFAAGDMRTGQSLIVRCINEGRECAREVDAYLMGNTNLEAK
AGSLMLSAS

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=455, Percent_Identity=24.1758241758242, Blast_Score=99, Evalue=7e-21,
Organism=Escherichia coli, GI1789606, Length=469, Percent_Identity=33.2622601279318, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1788811, Length=448, Percent_Identity=32.3660714285714, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI87082180, Length=441, Percent_Identity=32.6530612244898, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1788468, Length=435, Percent_Identity=26.4367816091954, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1789243, Length=430, Percent_Identity=29.7674418604651, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17570289, Length=498, Percent_Identity=43.7751004016064, Blast_Score=426, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI71984108, Length=473, Percent_Identity=22.8329809725159, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17543860, Length=169, Percent_Identity=34.9112426035503, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6320030, Length=493, Percent_Identity=45.2332657200811, Blast_Score=434, Evalue=1e-122,
Organism=Drosophila melanogaster, GI28574881, Length=485, Percent_Identity=48.4536082474227, Blast_Score=465, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24665539, Length=485, Percent_Identity=48.4536082474227, Blast_Score=465, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24665547, Length=485, Percent_Identity=48.4536082474227, Blast_Score=462, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24665543, Length=485, Percent_Identity=48.4536082474227, Blast_Score=462, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24640763, Length=285, Percent_Identity=26.6666666666667, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI18858217, Length=285, Percent_Identity=26.6666666666667, Blast_Score=93, Evalue=4e-19,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53601; Mature: 53470

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKPTGFLENGRELPKKLEPIVRIQDNKEFVLNEEFGDKINTQASRCMDCGVPFCHNGCP
CCCCCCCHHCCHHHHHHCCCEEEEECCCEEEEECHHCCHHHHHHHHHHHCCCCCCCCCCC
IGNIIPEFNDAVYRDSWEEAWHILSSTNNFPEFTGRVCPAPCESACVLGINQDPITICNI
CCCCCCCCCCHHHHCCHHHHHHHHHCCCCCCHHCCCCCCCCCCCEEEEECCCCCEEEECC
EKTIVETAYREGYAQPKKPRSRTGKTVAIIGSGPAGLAAAEQINSAGHSVTVFERDEKVG
HHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCC
GLLRFGIPDFKLGMDIIDRKINLMAEAGIKFEVNAHIGVSINAQQLRQDFDVVLLTGGST
CEEEECCCCHHHCHHHHHHHHHHHHHCCEEEEEEEEEEEEECHHHHHCCCCEEEEECCCC
VPRDLPIPGRELNGVHFAMEFLGQNNRRANNMDLKTKEIHAKDKHVVVIGGGDTGSDCVG
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEHEECCCCCEEEEEECCCCCCCCCC
TSNRHGAASITQVEIMPIPPEKRPANMPWPQYPMIMKTTTSHEEGCERHWNILTKEFISD
CCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCC
DEGNVTGLRIADIVWQDAKPGERPGFNEVANSERVIPCDQAFLAMGFLHPEPTGVLAQLD
CCCCEEEEEEEEEEECCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEE
IKLDDRGNVATNGFSTNQKGVFAAGDMRTGQSLIVRCINEGRECAREVDAYLMGNTNLEA
EEECCCCCEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
KAGSLMLSAS
CCCCEEEECC
>Mature Secondary Structure 
GKPTGFLENGRELPKKLEPIVRIQDNKEFVLNEEFGDKINTQASRCMDCGVPFCHNGCP
CCCCCCHHCCHHHHHHCCCEEEEECCCEEEEECHHCCHHHHHHHHHHHCCCCCCCCCCC
IGNIIPEFNDAVYRDSWEEAWHILSSTNNFPEFTGRVCPAPCESACVLGINQDPITICNI
CCCCCCCCCCHHHHCCHHHHHHHHHCCCCCCHHCCCCCCCCCCCEEEEECCCCCEEEECC
EKTIVETAYREGYAQPKKPRSRTGKTVAIIGSGPAGLAAAEQINSAGHSVTVFERDEKVG
HHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCC
GLLRFGIPDFKLGMDIIDRKINLMAEAGIKFEVNAHIGVSINAQQLRQDFDVVLLTGGST
CEEEECCCCHHHCHHHHHHHHHHHHHCCEEEEEEEEEEEEECHHHHHCCCCEEEEECCCC
VPRDLPIPGRELNGVHFAMEFLGQNNRRANNMDLKTKEIHAKDKHVVVIGGGDTGSDCVG
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEHEECCCCCEEEEEECCCCCCCCCC
TSNRHGAASITQVEIMPIPPEKRPANMPWPQYPMIMKTTTSHEEGCERHWNILTKEFISD
CCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCC
DEGNVTGLRIADIVWQDAKPGERPGFNEVANSERVIPCDQAFLAMGFLHPEPTGVLAQLD
CCCCEEEEEEEEEEECCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEE
IKLDDRGNVATNGFSTNQKGVFAAGDMRTGQSLIVRCINEGRECAREVDAYLMGNTNLEA
EEECCCCCEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
KAGSLMLSAS
CCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]