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The map label for this gene is int [H]

Identifier: 209695900

GI number: 209695900

Start: 2654415

End: 2655656

Strand: Direct

Name: int [H]

Synonym: VSAL_I2480

Alternate gene names: 209695900

Gene position: 2654415-2655656 (Clockwise)

Preceding gene: 209695899

Following gene: 209695903

Centisome position: 79.83

GC content: 37.92

Gene sequence:

>1242_bases
ATGGCATTAAAGACAACTCGCTTAAATAATAAACAAATTGATAATGCAAAAGAACGAGAGAAAGATTATGTCCTCTCTGA
TGGTGAAGGTCTTCAATTACGAATTCGTACTAATGGTTCTAAACTATGGAACTTCAACTACATTCATCCTCAAACTAAAA
AACGATTAAATATGGGGCTAGGTTCTTACCCTTCACTTAGTCTTGCGAATGCCAGAAAGCTAACCGTGTCTGCAAGAGAG
TTAGTTGCGCAAGGTATAGACCCTAAAGAACATCGTGATGAGAAAAAACACCAAGACCTGATAATTCATGAACATACTTT
TTTTAATGTTGCTACTGAATGGTTTGAGCGTAAGAAAGATGACATCACTGTAGATTATGCTCAAGACGTATGGCGATCTT
TAGAGCTACATATATTCCCTATACTTAAAAGTACACCTGTATCTCAAATTACAGCACCAATGATTATTGAATTACTTCGA
CCAATTGAAGCGAAAGGAAGTTTAGAAACAGTTAAACGACTGACTCAACGATTAAATGAGATCATGACCTATTCAGTAAA
CTGTGGGCTTATTCATGCTAACCCTCTTGCAGGGATCAAAGCTGCTTTTAAGAAACCCAAAAAAGAAAATATGGCGGCTC
TTAAACCTGATGAGTTACCAGAACTTATGATAGCCATTGCTAATGCTTCTATTAAGCGTACTACTCGTTGTTTAATTGAA
TGGCAACTTCATACAATGACTCGACCATCAGAAGCCGCTGGAACACGTTGGGAAGAAATAGATTTAGAGAAAGGAATTTG
GACAATTCCACCAGAGAGAATGAAAAAGCGTAGGGAACATCGTATACCTTTAACTGCTCAAGCTCTTTCTTTACTAGAAG
TTATGAAACCTATTAGTGCTCATAGAGAATTTGTATTCCCTTCAGATAGAGCACCATTAAAACCATCAAATAGCCAAACA
GCAAATATGGCAATAAAACGTATGGGTTTTGCTGGTAGGTTAGTAAGTCATGGATTACGTTCAGTAGCCAGTACTACGCT
AAACGAACAAGGCTTTGAATCAGACCTAATTGAAGCGGCTCTTGCCCACGTAGATGACAGTCAAGTACGTGCGGCTTATA
ACCGTACTGATTATCTTGAACGAAGAATACCGATGATGAAGTGGTGGAGTGGACATATTGAGGAAGCATCGAAAGGAAGT
TTATCTATATCAGGAATGAAGTGTTTATCTTTGGTTGGATAA

Upstream 100 bases:

>100_bases
CGTCCAATAACATCCAGTTACTTCCATCATTTCATGTATTCCTTTATGTATTCCAAGTCTATAATAGAGATCTGTGGAAT
ACATGATTGAGGGATTTACC

Downstream 100 bases:

>100_bases
GCGGCATAGATAAGGCTCCCGAAGGAGCCTTATCTCAATCTAACTGATTAGACATCAACTACTTTTTATGACCCTAAACG
ATAAAAAGGAACTTCAGCTC

Product: integrase, phage family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MALKTTRLNNKQIDNAKEREKDYVLSDGEGLQLRIRTNGSKLWNFNYIHPQTKKRLNMGLGSYPSLSLANARKLTVSARE
LVAQGIDPKEHRDEKKHQDLIIHEHTFFNVATEWFERKKDDITVDYAQDVWRSLELHIFPILKSTPVSQITAPMIIELLR
PIEAKGSLETVKRLTQRLNEIMTYSVNCGLIHANPLAGIKAAFKKPKKENMAALKPDELPELMIAIANASIKRTTRCLIE
WQLHTMTRPSEAAGTRWEEIDLEKGIWTIPPERMKKRREHRIPLTAQALSLLEVMKPISAHREFVFPSDRAPLKPSNSQT
ANMAIKRMGFAGRLVSHGLRSVASTTLNEQGFESDLIEAALAHVDDSQVRAAYNRTDYLERRIPMMKWWSGHIEEASKGS
LSISGMKCLSLVG

Sequences:

>Translated_413_residues
MALKTTRLNNKQIDNAKEREKDYVLSDGEGLQLRIRTNGSKLWNFNYIHPQTKKRLNMGLGSYPSLSLANARKLTVSARE
LVAQGIDPKEHRDEKKHQDLIIHEHTFFNVATEWFERKKDDITVDYAQDVWRSLELHIFPILKSTPVSQITAPMIIELLR
PIEAKGSLETVKRLTQRLNEIMTYSVNCGLIHANPLAGIKAAFKKPKKENMAALKPDELPELMIAIANASIKRTTRCLIE
WQLHTMTRPSEAAGTRWEEIDLEKGIWTIPPERMKKRREHRIPLTAQALSLLEVMKPISAHREFVFPSDRAPLKPSNSQT
ANMAIKRMGFAGRLVSHGLRSVASTTLNEQGFESDLIEAALAHVDDSQVRAAYNRTDYLERRIPMMKWWSGHIEEASKGS
LSISGMKCLSLVG
>Mature_412_residues
ALKTTRLNNKQIDNAKEREKDYVLSDGEGLQLRIRTNGSKLWNFNYIHPQTKKRLNMGLGSYPSLSLANARKLTVSAREL
VAQGIDPKEHRDEKKHQDLIIHEHTFFNVATEWFERKKDDITVDYAQDVWRSLELHIFPILKSTPVSQITAPMIIELLRP
IEAKGSLETVKRLTQRLNEIMTYSVNCGLIHANPLAGIKAAFKKPKKENMAALKPDELPELMIAIANASIKRTTRCLIEW
QLHTMTRPSEAAGTRWEEIDLEKGIWTIPPERMKKRREHRIPLTAQALSLLEVMKPISAHREFVFPSDRAPLKPSNSQTA
NMAIKRMGFAGRLVSHGLRSVASTTLNEQGFESDLIEAALAHVDDSQVRAAYNRTDYLERRIPMMKWWSGHIEEASKGSL
SISGMKCLSLVG

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=413, Percent_Identity=50.363196125908, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI1788690, Length=392, Percent_Identity=31.1224489795918, Blast_Score=201, Evalue=6e-53,
Organism=Escherichia coli, GI145693166, Length=404, Percent_Identity=33.4158415841584, Blast_Score=196, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 46959; Mature: 46828

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALKTTRLNNKQIDNAKEREKDYVLSDGEGLQLRIRTNGSKLWNFNYIHPQTKKRLNMGL
CCCCCCCCCCCCCCCHHHHCCCCEEECCCCEEEEEEECCCEEEEEEEECCHHHHHHHCCC
GSYPSLSLANARKLTVSARELVAQGIDPKEHRDEKKHQDLIIHEHTFFNVATEWFERKKD
CCCCCCCCCCCCEEEHHHHHHHHCCCCCHHHHHHHHHCCEEEECHHHHHHHHHHHHCCCC
DITVDYAQDVWRSLELHIFPILKSTPVSQITAPMIIELLRPIEAKGSLETVKRLTQRLNE
CEEEEHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
IMTYSVNCGLIHANPLAGIKAAFKKPKKENMAALKPDELPELMIAIANASIKRTTRCLIE
HHEEECCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHEE
WQLHTMTRPSEAAGTRWEEIDLEKGIWTIPPERMKKRREHRIPLTAQALSLLEVMKPISA
EEHHHCCCCCHHHCCCCHHEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
HREFVFPSDRAPLKPSNSQTANMAIKRMGFAGRLVSHGLRSVASTTLNEQGFESDLIEAA
HCCEECCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LAHVDDSQVRAAYNRTDYLERRIPMMKWWSGHIEEASKGSLSISGMKCLSLVG
HHHCCHHHHHHHHHHHHHHHHHCCHHHHHCCHHCCCCCCCEEECHHHHHHHCC
>Mature Secondary Structure 
ALKTTRLNNKQIDNAKEREKDYVLSDGEGLQLRIRTNGSKLWNFNYIHPQTKKRLNMGL
CCCCCCCCCCCCCCHHHHCCCCEEECCCCEEEEEEECCCEEEEEEEECCHHHHHHHCCC
GSYPSLSLANARKLTVSARELVAQGIDPKEHRDEKKHQDLIIHEHTFFNVATEWFERKKD
CCCCCCCCCCCCEEEHHHHHHHHCCCCCHHHHHHHHHCCEEEECHHHHHHHHHHHHCCCC
DITVDYAQDVWRSLELHIFPILKSTPVSQITAPMIIELLRPIEAKGSLETVKRLTQRLNE
CEEEEHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
IMTYSVNCGLIHANPLAGIKAAFKKPKKENMAALKPDELPELMIAIANASIKRTTRCLIE
HHEEECCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHEE
WQLHTMTRPSEAAGTRWEEIDLEKGIWTIPPERMKKRREHRIPLTAQALSLLEVMKPISA
EEHHHCCCCCHHHCCCCHHEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
HREFVFPSDRAPLKPSNSQTANMAIKRMGFAGRLVSHGLRSVASTTLNEQGFESDLIEAA
HCCEECCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LAHVDDSQVRAAYNRTDYLERRIPMMKWWSGHIEEASKGSLSISGMKCLSLVG
HHHCCHHHHHHHHHHHHHHHHHCCHHHHHCCHHCCCCCCCEEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7511583; 9278503; 9205837; 7524073 [H]