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The map label for this gene is smpB

Identifier: 209695899

GI number: 209695899

Start: 2653300

End: 2653785

Strand: Direct

Name: smpB

Synonym: VSAL_I2479

Alternate gene names: 209695899

Gene position: 2653300-2653785 (Clockwise)

Preceding gene: 209695896

Following gene: 209695900

Centisome position: 79.79

GC content: 40.74

Gene sequence:

>486_bases
ATGGCAAAGAAGAAATCAAAAGATAAAGCGGGCAGTAATACCATTGCTATGAACAAGCAAGCTCGCCACGAATATTTCAT
TGAAGATGAAATCGAAGCTGGGGTCGAACTCCAAGGCTGGGAAGTTAAATCTCTTCGTTCTGGTAAAGTAAATATCGCGG
AAAGCTACGTATATGTTCGCGATGGCGAAATTTTCATTAGTGGTATGAACATAACACCACTGCAAGCAGCATCGACTCAT
GTTGTTGCGAACCCTACTCGTGTTCGTAAACTTTTAATGAGCCGTAAAGAAATTGATAATCTAATCGGTCGTGTGAACCG
TGAAGGTATGACGCTTGTTGCTACTACCATGTACTGGGTTCGCTCTTGGGCGAAAATAAAAGTTGGTGTAGCAAAAGGTA
AGAAACTGCACGATAAACGTACAGATTCAAAAGAAAAAGACTGGAACAGAGATAAAGCTCGAATTATGAAGAGTAGTTTG
CGTTAA

Upstream 100 bases:

>100_bases
AATTCTCCTTTACATAATAGAGGGTTGCATCATACCATCTTGATGTTAAGAGCCAAACTACTTCCCCTATTCTCAACTAA
TAACGTATAATGTGGGCATT

Downstream 100 bases:

>100_bases
TTTCAACCAGTTAGACTGATACTAACTAGACAGACGCTGTTTTTCTGTTAGTATCATCAGGTACCAAATCTTTGGGGCTG
ATTCAGGATTCGACGGGAAT

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 161; Mature: 160

Protein sequence:

>161_residues
MAKKKSKDKAGSNTIAMNKQARHEYFIEDEIEAGVELQGWEVKSLRSGKVNIAESYVYVRDGEIFISGMNITPLQAASTH
VVANPTRVRKLLMSRKEIDNLIGRVNREGMTLVATTMYWVRSWAKIKVGVAKGKKLHDKRTDSKEKDWNRDKARIMKSSL
R

Sequences:

>Translated_161_residues
MAKKKSKDKAGSNTIAMNKQARHEYFIEDEIEAGVELQGWEVKSLRSGKVNIAESYVYVRDGEIFISGMNITPLQAASTH
VVANPTRVRKLLMSRKEIDNLIGRVNREGMTLVATTMYWVRSWAKIKVGVAKGKKLHDKRTDSKEKDWNRDKARIMKSSL
R
>Mature_160_residues
AKKKSKDKAGSNTIAMNKQARHEYFIEDEIEAGVELQGWEVKSLRSGKVNIAESYVYVRDGEIFISGMNITPLQAASTHV
VANPTRVRKLLMSRKEIDNLIGRVNREGMTLVATTMYWVRSWAKIKVGVAKGKKLHDKRTDSKEKDWNRDKARIMKSSLR

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=161, Percent_Identity=65.2173913043478, Blast_Score=226, Evalue=5e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_ALISL (B6EKA9)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263829.1
- GeneID:   6987776
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2479
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 18367; Mature: 18235

Theoretical pI: Translated: 10.76; Mature: 10.76

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKKSKDKAGSNTIAMNKQARHEYFIEDEIEAGVELQGWEVKSLRSGKVNIAESYVYVR
CCCCCCCCCCCCCEEEECCCCHHCCCCCCHHCCCCEECCCEECCCCCCCEEEEEEEEEEE
DGEIFISGMNITPLQAASTHVVANPTRVRKLLMSRKEIDNLIGRVNREGMTLVATTMYWV
CCEEEEECCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHH
RSWAKIKVGVAKGKKLHDKRTDSKEKDWNRDKARIMKSSLR
HHHHHEEEECCCCCHHHCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
AKKKSKDKAGSNTIAMNKQARHEYFIEDEIEAGVELQGWEVKSLRSGKVNIAESYVYVR
CCCCCCCCCCCCEEEECCCCHHCCCCCCHHCCCCEECCCEECCCCCCCEEEEEEEEEEE
DGEIFISGMNITPLQAASTHVVANPTRVRKLLMSRKEIDNLIGRVNREGMTLVATTMYWV
CCEEEEECCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHH
RSWAKIKVGVAKGKKLHDKRTDSKEKDWNRDKARIMKSSLR
HHHHHEEEECCCCCHHHCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA