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The map label for this gene is purR [H]

Identifier: 209695886

GI number: 209695886

Start: 2638373

End: 2639383

Strand: Direct

Name: purR [H]

Synonym: VSAL_I2465

Alternate gene names: 209695886

Gene position: 2638373-2639383 (Clockwise)

Preceding gene: 209695885

Following gene: 209695887

Centisome position: 79.35

GC content: 37.59

Gene sequence:

>1011_bases
ATGACGCTTCCAAAATTAATGATAACGTCTAAAGACGTAGCAAAACTTGCCGGAGTTTCTCAATCTACGGTTTCTCGTGT
TTTTATTCCTGGTAGCTCCGCATCAGAAAAAACAAAGCAAAAGGTATTTGAAGCAGCAAAAGCCTTAAACTACCGACCAA
ATGCATTTGCCCGCAGTTTAACGACCAATGAATCGAAACTTATTGGTTTAGTTTTTCCTGATGCCGACTACCCAATCCAC
ATGAAAACACTGCAATTAATCTCTAATGAATTACAGAAAAAAGGATTTTCAGCCGTATTGATCCCATGGCAACTAGATGA
AGATAAACAACATTCAATTCCTAATATCTTTCAGTACCGAGTTGATGGTGTCATTGCCGCTTCAGCAACGCTCAACAAAT
CGTTATACGAAGAATGTGAAGAGTTTAATATTCCTATTATTCAATACGCCCGAGTAGTCGAAGGAACAAAAAGCAGTTAT
GTCATTAGTGATAACTACACCGCAGGTCAAATAGCCGCTCAACATTTTCATAAAAGCAATGTGAAATCGGCATTATATTT
AACTGGTGAAGTACCTACATTTACTAACGACGAACGTAAATTTGGTTTTTGCACGACTTTCGAAGAATTAACAGGGAATA
AAGCTCAAGTAGTCGAAGCAACTTATGATTATTCTGATTCAATCGATACTATTCGACAATTATTTTCTCAACCTCATCGA
CCTGAAGCTATCTTTTGCGCGACGGATAATTTAGCGATGGCAGCCATGGATGTGGCTCGTTTAGAGTGCGGCTTGCGCAT
ACCAGAAGATCTTCAAGTAATTGGATTCGATAATATTCCTCAAACCGAGTGGCTAAATTATCAACTCACGACCTTTAAAC
AAGATTTTCAACGTTTAGCACGTGAATCCGTTAAGATCATTGTAGATCAGATAGAAAACAAAAATACAAACCTAGTAAGA
TTAATGATACCAGTCGCTTTTGTTGAACGAAAAACGACAATACAAAGTTAA

Upstream 100 bases:

>100_bases
TGAGGCACTCTCCCATCACCGTTTAGACGTTGAGTTTATGAATACCATTTTATTCTATGGTAGACTCTAAGCAACTTAAT
TGACTGATAATCAATAAAAT

Downstream 100 bases:

>100_bases
TTGAATCACATCCAAAAGAGATAAGGCCTTTATAAACACGAAAGATCATATCTCTAGCACTTCCCTTCCTTTCCATGTAA
AATCCGGCCTCCTTCATATC

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MTLPKLMITSKDVAKLAGVSQSTVSRVFIPGSSASEKTKQKVFEAAKALNYRPNAFARSLTTNESKLIGLVFPDADYPIH
MKTLQLISNELQKKGFSAVLIPWQLDEDKQHSIPNIFQYRVDGVIAASATLNKSLYEECEEFNIPIIQYARVVEGTKSSY
VISDNYTAGQIAAQHFHKSNVKSALYLTGEVPTFTNDERKFGFCTTFEELTGNKAQVVEATYDYSDSIDTIRQLFSQPHR
PEAIFCATDNLAMAAMDVARLECGLRIPEDLQVIGFDNIPQTEWLNYQLTTFKQDFQRLARESVKIIVDQIENKNTNLVR
LMIPVAFVERKTTIQS

Sequences:

>Translated_336_residues
MTLPKLMITSKDVAKLAGVSQSTVSRVFIPGSSASEKTKQKVFEAAKALNYRPNAFARSLTTNESKLIGLVFPDADYPIH
MKTLQLISNELQKKGFSAVLIPWQLDEDKQHSIPNIFQYRVDGVIAASATLNKSLYEECEEFNIPIIQYARVVEGTKSSY
VISDNYTAGQIAAQHFHKSNVKSALYLTGEVPTFTNDERKFGFCTTFEELTGNKAQVVEATYDYSDSIDTIRQLFSQPHR
PEAIFCATDNLAMAAMDVARLECGLRIPEDLQVIGFDNIPQTEWLNYQLTTFKQDFQRLARESVKIIVDQIENKNTNLVR
LMIPVAFVERKTTIQS
>Mature_335_residues
TLPKLMITSKDVAKLAGVSQSTVSRVFIPGSSASEKTKQKVFEAAKALNYRPNAFARSLTTNESKLIGLVFPDADYPIHM
KTLQLISNELQKKGFSAVLIPWQLDEDKQHSIPNIFQYRVDGVIAASATLNKSLYEECEEFNIPIIQYARVVEGTKSSYV
ISDNYTAGQIAAQHFHKSNVKSALYLTGEVPTFTNDERKFGFCTTFEELTGNKAQVVEATYDYSDSIDTIRQLFSQPHRP
EAIFCATDNLAMAAMDVARLECGLRIPEDLQVIGFDNIPQTEWLNYQLTTFKQDFQRLARESVKIIVDQIENKNTNLVRL
MIPVAFVERKTTIQS

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=331, Percent_Identity=28.7009063444109, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1790194, Length=330, Percent_Identity=27.2727272727273, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1788474, Length=310, Percent_Identity=29.3548387096774, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1790369, Length=330, Percent_Identity=26.0606060606061, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1786540, Length=333, Percent_Identity=28.2282282282282, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1787580, Length=317, Percent_Identity=26.813880126183, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789202, Length=311, Percent_Identity=22.8295819935691, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789068, Length=294, Percent_Identity=25.5102040816327, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787906, Length=252, Percent_Identity=28.5714285714286, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1786268, Length=309, Percent_Identity=23.3009708737864, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI48994940, Length=318, Percent_Identity=23.2704402515723, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1790715, Length=316, Percent_Identity=21.5189873417722, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790689, Length=318, Percent_Identity=21.6981132075472, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37843; Mature: 37711

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPKLMITSKDVAKLAGVSQSTVSRVFIPGSSASEKTKQKVFEAAKALNYRPNAFARSL
CCCCCCEECCHHHHHHHCCCHHHHCEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHC
TTNESKLIGLVFPDADYPIHMKTLQLISNELQKKGFSAVLIPWQLDEDKQHSIPNIFQYR
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHH
VDGVIAASATLNKSLYEECEEFNIPIIQYARVVEGTKSSYVISDNYTAGQIAAQHFHKSN
HCEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCC
VKSALYLTGEVPTFTNDERKFGFCTTFEELTGNKAQVVEATYDYSDSIDTIRQLFSQPHR
CCEEEEEECCCCCCCCCCCCCEEEEEHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCCCC
PEAIFCATDNLAMAAMDVARLECGLRIPEDLQVIGFDNIPQTEWLNYQLTTFKQDFQRLA
CCEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCEEHHHHHHHHHHHH
RESVKIIVDQIENKNTNLVRLMIPVAFVERKTTIQS
HHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCC
>Mature Secondary Structure 
TLPKLMITSKDVAKLAGVSQSTVSRVFIPGSSASEKTKQKVFEAAKALNYRPNAFARSL
CCCCCEECCHHHHHHHCCCHHHHCEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHC
TTNESKLIGLVFPDADYPIHMKTLQLISNELQKKGFSAVLIPWQLDEDKQHSIPNIFQYR
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHH
VDGVIAASATLNKSLYEECEEFNIPIIQYARVVEGTKSSYVISDNYTAGQIAAQHFHKSN
HCEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCC
VKSALYLTGEVPTFTNDERKFGFCTTFEELTGNKAQVVEATYDYSDSIDTIRQLFSQPHR
CCEEEEEECCCCCCCCCCCCCEEEEEHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCCCC
PEAIFCATDNLAMAAMDVARLECGLRIPEDLQVIGFDNIPQTEWLNYQLTTFKQDFQRLA
CCEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCEEHHHHHHHHHHHH
RESVKIIVDQIENKNTNLVRLMIPVAFVERKTTIQS
HHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA