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The map label for this gene is purN [H]

Identifier: 209695816

GI number: 209695816

Start: 2560436

End: 2561080

Strand: Direct

Name: purN [H]

Synonym: VSAL_I2391

Alternate gene names: 209695816

Gene position: 2560436-2561080 (Clockwise)

Preceding gene: 209695815

Following gene: 209695819

Centisome position: 77.0

GC content: 40.16

Gene sequence:

>645_bases
ATGATGAAGAATATTGTTGTATTAGTTTCAGGAAACGGCAGTAACCTACAAGAGTTTATCGATGCTTGTGGTAACAAAAT
TCCAAATGCACGCATCTCTGCGGTTATTTCAAATAAAAGCGATGCTTATGGACTACAACGTGCAATTAATGCCGATATTG
ATGTACACAGCTTAAGTGCTGCGGGCTATGAAGGTCGTGAGCAGTACGATATCGCACTATCTACTCTTATCGATCTTTAT
CAGCCTGATTTAATTATTCTGGCCGGTTTTATGCGTATTTTAAGTGCAGATTTTGTCTTACGTTATCAAGGTAAGATGCT
GAACATCCACCCCTCTCTTCTACCGAAATATACAGGATTACATACTCACCAACGTGCGATTGACGCCGGAGATGAAGAAC
ATGGTACAAGTGTTCATTTTGTCACGCCAGAGCTCGATGGAGGTCCTGTTATTCTTCAAGCAAAAGTGCCTATTTTTGAT
GAAGATACTGCTGAAGATGTCGCTTTACGTGTTCAAGCTCAAGAACACGTCATTTACCCTATGGTTGCTAATTGGATTAT
AGAAGAGCGTTTAATAATGACAGATGGAAAAGCAGTTCTTGATGGAGATGCGCTAGGGACATTTGGATTAGAAACAGAAG
AGTAA

Upstream 100 bases:

>100_bases
CATAAAGAATAAATAAAAGGTGATCAATTATTCGGTCACCTTTTTTATATCTGTAATTTGTGTTCGAATGGTTCTCATTA
AACAATAACTAAAAAAGATT

Downstream 100 bases:

>100_bases
AATAATATCACTTAGTAAAAACGTCATCTTAAACCATAAGATGACGTTTATATTTTATTATGTATTTACTGGACACTCAG
AAGGCGGCGGCGCCATATCA

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MMKNIVVLVSGNGSNLQEFIDACGNKIPNARISAVISNKSDAYGLQRAINADIDVHSLSAAGYEGREQYDIALSTLIDLY
QPDLIILAGFMRILSADFVLRYQGKMLNIHPSLLPKYTGLHTHQRAIDAGDEEHGTSVHFVTPELDGGPVILQAKVPIFD
EDTAEDVALRVQAQEHVIYPMVANWIIEERLIMTDGKAVLDGDALGTFGLETEE

Sequences:

>Translated_214_residues
MMKNIVVLVSGNGSNLQEFIDACGNKIPNARISAVISNKSDAYGLQRAINADIDVHSLSAAGYEGREQYDIALSTLIDLY
QPDLIILAGFMRILSADFVLRYQGKMLNIHPSLLPKYTGLHTHQRAIDAGDEEHGTSVHFVTPELDGGPVILQAKVPIFD
EDTAEDVALRVQAQEHVIYPMVANWIIEERLIMTDGKAVLDGDALGTFGLETEE
>Mature_214_residues
MMKNIVVLVSGNGSNLQEFIDACGNKIPNARISAVISNKSDAYGLQRAINADIDVHSLSAAGYEGREQYDIALSTLIDLY
QPDLIILAGFMRILSADFVLRYQGKMLNIHPSLLPKYTGLHTHQRAIDAGDEEHGTSVHFVTPELDGGPVILQAKVPIFD
EDTAEDVALRVQAQEHVIYPMVANWIIEERLIMTDGKAVLDGDALGTFGLETEE

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=177, Percent_Identity=42.3728813559322, Blast_Score=150, Evalue=6e-37,
Organism=Homo sapiens, GI209869995, Length=177, Percent_Identity=42.3728813559322, Blast_Score=150, Evalue=6e-37,
Organism=Homo sapiens, GI209869993, Length=177, Percent_Identity=42.3728813559322, Blast_Score=150, Evalue=6e-37,
Organism=Escherichia coli, GI1788846, Length=211, Percent_Identity=58.7677725118483, Blast_Score=256, Evalue=9e-70,
Organism=Escherichia coli, GI1787483, Length=165, Percent_Identity=32.7272727272727, Blast_Score=91, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17567511, Length=176, Percent_Identity=34.6590909090909, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6320616, Length=199, Percent_Identity=35.1758793969849, Blast_Score=91, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24582400, Length=180, Percent_Identity=45.5555555555556, Blast_Score=159, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23447; Mature: 23447

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKNIVVLVSGNGSNLQEFIDACGNKIPNARISAVISNKSDAYGLQRAINADIDVHSLSA
CCCEEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECC
AGYEGREQYDIALSTLIDLYQPDLIILAGFMRILSADFVLRYQGKMLNIHPSLLPKYTGL
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCEEEEECCCEEEECHHHCCCCCCC
HTHQRAIDAGDEEHGTSVHFVTPELDGGPVILQAKVPIFDEDTAEDVALRVQAQEHVIYP
CCHHHHHCCCCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCEEEHH
MVANWIIEERLIMTDGKAVLDGDALGTFGLETEE
HHHHHHHHCEEEEECCCEEECCCCCEECCCCCCC
>Mature Secondary Structure
MMKNIVVLVSGNGSNLQEFIDACGNKIPNARISAVISNKSDAYGLQRAINADIDVHSLSA
CCCEEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECC
AGYEGREQYDIALSTLIDLYQPDLIILAGFMRILSADFVLRYQGKMLNIHPSLLPKYTGL
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCEEEEECCCEEEECHHHCCCCCCC
HTHQRAIDAGDEEHGTSVHFVTPELDGGPVILQAKVPIFDEDTAEDVALRVQAQEHVIYP
CCHHHHHCCCCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCEEEHH
MVANWIIEERLIMTDGKAVLDGDALGTFGLETEE
HHHHHHHHCEEEEECCCEEECCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]