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The map label for this gene is hlyB [H]

Identifier: 209695571

GI number: 209695571

Start: 2276649

End: 2278289

Strand: Reverse

Name: hlyB [H]

Synonym: VSAL_I2130

Alternate gene names: 209695571

Gene position: 2278289-2276649 (Counterclockwise)

Preceding gene: 209695572

Following gene: 209695567

Centisome position: 68.52

GC content: 37.84

Gene sequence:

>1641_bases
ATGAAGTTAGCAGGACGTTTATCAGTAAAAACAAAAATAGCATTGCCAATTGTCGCGATTGTTTCTTTATTCAGTGTTAT
CTCAACTCTTAATGTGATGAAGCTAAATGAACAAGCGGAGGTTAATTACAAATTAATTGAGCTTGTTCAGCCAGTAAATG
AATCCTTAGAAGATGCATACCGAGATCTTTATCAAGTTACTGCAGCAGCGCAAGGTTTAATGCTTTCAACAACCAATGCA
GAAAGAGAGCATCATACGTTTGAATTTCAAGATAACGCCTACAAAGCCATTCCTCGTTTAAAAAAGGTTGAAGTGTTATA
TCAAGAAAATATTTTGCCTTTAAATACTCAAGTTGAATTAACAACGTTAATTAATGCGGCCGAAAAATGGATTGAACTTT
ATGAGCCTCTGTTCTCGGACCCTAATAATGCTCAAGCTTATTATCAGAAAAATAATAATCAGTTAGACGAACAATTCAAG
ATCATGCGTAAGCAACTTAAATCAATCAGTAAGTTGATTGTGAGTGAGCAAGTAAAGTTAGGTGAACAAAGTCATTATGG
GATTAATTCGGGGAAAATTATTTCAGGAATTGGTGCCATTAGTGCTGTGATTTTTGGCTTATTTGCTGTTTGGTTATCAA
TTAACTGGATTGTAAAACCAATTCAATCATTAGAATTGGCTATGTCAGAAGTCGCTTCTGGAGACGGAGATTTAACAAAG
CGCATCGCCGTTACCTCAAATGATGAAATTGGCAAACTTGCACAAGCCTTTAATTTGTTTGTATCAAAGATACATACCAC
GGTTTCAGATGTTATTGCATCGTCTAATACCGTTCGTTCTGAAATGGACAATATCCAAGTATTAACAAAAAATATAGCTA
AATTCTCTTCAAGCCAACAACAAGAGAGTGAAGTTGTTGCTGCTGCTGTGCATGAAATGCAAGTAACAAGTAATACGGTG
AATGAAAATGCCAATGAAGCAGCCCAAGCAAGCTTAGGCGCCACAGCAGAAGCTGATACGACAGAAGCTATTTTACAGCA
GACCGTAATATCGATTCAAGGATTGGCCAATGAGATAACATTGGCGGGTACTGTAATTCATACTCTAGATAGTGATGTTT
CAAATATAGCATCGATTTTAGGTGTAATTAAAGGGATAGCTGACCAAACAAATTTATTAGCATTAAATGCGGCAATAGAA
GCTGCGCGAGCTGGTGAGCAAGGACGTGGTTTTGCGGTTGTTGCTGATGAAGTAAGGGCGTTAGCAAGTAAAACGCAATA
CAGTACTGGTGAGATCGAAAAGATGATTGAGCGTTTACAATCGGGAGCAAAAGAAGCTGTTCTCGCAATGGAATCAAGTA
CCAAAAGTGGTAAAGAAACGATTGATCTAGCAAGTGAGGCATCAAAGTCGTTACAACATATCACAAGAGCGATTGTAGTT
ATGAACGATATGAATACGCATATAGCCACAGCCGCTAATGAGCAGAGCCATGTTAGTGATGACGTCAACTCGAATGTACA
ACGTATTGCAGATAATAGTTCAGAAATGGTGCTGATGGTAAGTCGCTCAGAAAGTGCTTGTATTTCACTATCAGAACAGT
GCGAGAGACTTGATGGTTTGGTCTCTCAATTTAAAGTGTAA

Upstream 100 bases:

>100_bases
TAGCGAAACTTATTTGCATGAGTTATGGGTTTGGTCAATTTATATTTAGCGTTATTTGGTATAGTTCTGTTGAATAATTT
TTATTTCATATGGATAGGTT

Downstream 100 bases:

>100_bases
TCATAAAATAGAGATTGAGTGTTCGTCGAATAATTAGCAAAAATGGCTTACTGGTGTAAGCCATTTTTATTTATCATCAG
TATTCAGTATTGCATTTATT

Product: methyl-accepting chemotaxis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MKLAGRLSVKTKIALPIVAIVSLFSVISTLNVMKLNEQAEVNYKLIELVQPVNESLEDAYRDLYQVTAAAQGLMLSTTNA
EREHHTFEFQDNAYKAIPRLKKVEVLYQENILPLNTQVELTTLINAAEKWIELYEPLFSDPNNAQAYYQKNNNQLDEQFK
IMRKQLKSISKLIVSEQVKLGEQSHYGINSGKIISGIGAISAVIFGLFAVWLSINWIVKPIQSLELAMSEVASGDGDLTK
RIAVTSNDEIGKLAQAFNLFVSKIHTTVSDVIASSNTVRSEMDNIQVLTKNIAKFSSSQQQESEVVAAAVHEMQVTSNTV
NENANEAAQASLGATAEADTTEAILQQTVISIQGLANEITLAGTVIHTLDSDVSNIASILGVIKGIADQTNLLALNAAIE
AARAGEQGRGFAVVADEVRALASKTQYSTGEIEKMIERLQSGAKEAVLAMESSTKSGKETIDLASEASKSLQHITRAIVV
MNDMNTHIATAANEQSHVSDDVNSNVQRIADNSSEMVLMVSRSESACISLSEQCERLDGLVSQFKV

Sequences:

>Translated_546_residues
MKLAGRLSVKTKIALPIVAIVSLFSVISTLNVMKLNEQAEVNYKLIELVQPVNESLEDAYRDLYQVTAAAQGLMLSTTNA
EREHHTFEFQDNAYKAIPRLKKVEVLYQENILPLNTQVELTTLINAAEKWIELYEPLFSDPNNAQAYYQKNNNQLDEQFK
IMRKQLKSISKLIVSEQVKLGEQSHYGINSGKIISGIGAISAVIFGLFAVWLSINWIVKPIQSLELAMSEVASGDGDLTK
RIAVTSNDEIGKLAQAFNLFVSKIHTTVSDVIASSNTVRSEMDNIQVLTKNIAKFSSSQQQESEVVAAAVHEMQVTSNTV
NENANEAAQASLGATAEADTTEAILQQTVISIQGLANEITLAGTVIHTLDSDVSNIASILGVIKGIADQTNLLALNAAIE
AARAGEQGRGFAVVADEVRALASKTQYSTGEIEKMIERLQSGAKEAVLAMESSTKSGKETIDLASEASKSLQHITRAIVV
MNDMNTHIATAANEQSHVSDDVNSNVQRIADNSSEMVLMVSRSESACISLSEQCERLDGLVSQFKV
>Mature_546_residues
MKLAGRLSVKTKIALPIVAIVSLFSVISTLNVMKLNEQAEVNYKLIELVQPVNESLEDAYRDLYQVTAAAQGLMLSTTNA
EREHHTFEFQDNAYKAIPRLKKVEVLYQENILPLNTQVELTTLINAAEKWIELYEPLFSDPNNAQAYYQKNNNQLDEQFK
IMRKQLKSISKLIVSEQVKLGEQSHYGINSGKIISGIGAISAVIFGLFAVWLSINWIVKPIQSLELAMSEVASGDGDLTK
RIAVTSNDEIGKLAQAFNLFVSKIHTTVSDVIASSNTVRSEMDNIQVLTKNIAKFSSSQQQESEVVAAAVHEMQVTSNTV
NENANEAAQASLGATAEADTTEAILQQTVISIQGLANEITLAGTVIHTLDSDVSNIASILGVIKGIADQTNLLALNAAIE
AARAGEQGRGFAVVADEVRALASKTQYSTGEIEKMIERLQSGAKEAVLAMESSTKSGKETIDLASEASKSLQHITRAIVV
MNDMNTHIATAANEQSHVSDDVNSNVQRIADNSSEMVLMVSRSESACISLSEQCERLDGLVSQFKV

Specific function: May form a pore through which the hemolysin can be exported [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI2367378, Length=307, Percent_Identity=31.5960912052117, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1788194, Length=371, Percent_Identity=31.5363881401617, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1788195, Length=297, Percent_Identity=32.996632996633, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1787690, Length=379, Percent_Identity=30.0791556728232, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1789453, Length=315, Percent_Identity=32.3809523809524, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660
- InterPro:   IPR000727 [H]

Pfam domain/function: PF00672 HAMP; PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 59333; Mature: 59333

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2 ; PS50192 T_SNARE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAGRLSVKTKIALPIVAIVSLFSVISTLNVMKLNEQAEVNYKLIELVQPVNESLEDAY
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCHHHHHHHHHHHHHHHHHHH
RDLYQVTAAAQGLMLSTTNAEREHHTFEFQDNAYKAIPRLKKVEVLYQENILPLNTQVEL
HHHHHHHHHHCCCEEEECCCCCCCCCEEECCCHHHHHCHHHHHHHHHHCCCCCCCCCCHH
TTLINAAEKWIELYEPLFSDPNNAQAYYQKNNNQLDEQFKIMRKQLKSISKLIVSEQVKL
HHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GEQSHYGINSGKIISGIGAISAVIFGLFAVWLSINWIVKPIQSLELAMSEVASGDGDLTK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHE
RIAVTSNDEIGKLAQAFNLFVSKIHTTVSDVIASSNTVRSEMDNIQVLTKNIAKFSSSQQ
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHH
QESEVVAAAVHEMQVTSNTVNENANEAAQASLGATAEADTTEAILQQTVISIQGLANEIT
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LAGTVIHTLDSDVSNIASILGVIKGIADQTNLLALNAAIEAARAGEQGRGFAVVADEVRA
HHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCEEEEHHHHHH
LASKTQYSTGEIEKMIERLQSGAKEAVLAMESSTKSGKETIDLASEASKSLQHITRAIVV
HHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
MNDMNTHIATAANEQSHVSDDVNSNVQRIADNSSEMVLMVSRSESACISLSEQCERLDGL
EECCCCHHEECCCCHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
VSQFKV
HHHHCC
>Mature Secondary Structure
MKLAGRLSVKTKIALPIVAIVSLFSVISTLNVMKLNEQAEVNYKLIELVQPVNESLEDAY
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCHHHHHHHHHHHHHHHHHHH
RDLYQVTAAAQGLMLSTTNAEREHHTFEFQDNAYKAIPRLKKVEVLYQENILPLNTQVEL
HHHHHHHHHHCCCEEEECCCCCCCCCEEECCCHHHHHCHHHHHHHHHHCCCCCCCCCCHH
TTLINAAEKWIELYEPLFSDPNNAQAYYQKNNNQLDEQFKIMRKQLKSISKLIVSEQVKL
HHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GEQSHYGINSGKIISGIGAISAVIFGLFAVWLSINWIVKPIQSLELAMSEVASGDGDLTK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHE
RIAVTSNDEIGKLAQAFNLFVSKIHTTVSDVIASSNTVRSEMDNIQVLTKNIAKFSSSQQ
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHH
QESEVVAAAVHEMQVTSNTVNENANEAAQASLGATAEADTTEAILQQTVISIQGLANEIT
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LAGTVIHTLDSDVSNIASILGVIKGIADQTNLLALNAAIEAARAGEQGRGFAVVADEVRA
HHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCEEEEHHHHHH
LASKTQYSTGEIEKMIERLQSGAKEAVLAMESSTKSGKETIDLASEASKSLQHITRAIVV
HHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
MNDMNTHIATAANEQSHVSDDVNSNVQRIADNSSEMVLMVSRSESACISLSEQCERLDGL
EECCCCHHEECCCCHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
VSQFKV
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2162464; 10952301 [H]