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The map label for this gene is pcl [H]

Identifier: 209695544

GI number: 209695544

Start: 2250711

End: 2252084

Strand: Reverse

Name: pcl [H]

Synonym: VSAL_I2100

Alternate gene names: 209695544

Gene position: 2252084-2250711 (Counterclockwise)

Preceding gene: 209695545

Following gene: 209695543

Centisome position: 67.73

GC content: 40.17

Gene sequence:

>1374_bases
ATGACCTACACATCACTTTCTCATCTGACATTACAATCTAATGCCTCTCATTCCGTGTGTTTCGATGAACAACATAACGT
TACTGGTTATGAAAAGTTTCAACAAGACGTTCAAATTTTCAGTGCGTATTTAAGTGATAAACCTCAGAGAAAATGGGCGC
TGTGTTTTGAAAATAGCTATGCTTTTTCTGTGGCGTTTATGGCATTGGCTCATGCGGGTAAGCATCTTATTTTACCAGGA
AATCATCAACCCGCAGCCTTGACTGAATTGGCTCAACATTTTGATGCATTATTGCATGATGACATTATTGAAACGATGCC
TTGTGAGATCCATATTAACTACAATGCGGTAGATTTATCGATTCAACATACGGAAAAACTATCTCCTCTTTCTTTGTGTG
ATATCCCGTTAACGCTGTTTACATCGGGCTCAACCGATACCCCTAAACCCATTAATAAGACCCTGCAATTATTGGATAAC
GAAATACAACAATTAGAATTGTTGTGGGGTGAAAAAGTCAAAGACTCAGAATGTTATAGCACCGTTTCTCATCAACATAT
TTATGGCTTGTTGTTCCGAGTACTGTGGCCATTGTGTTCAGGACGCGCGTTTAGCCAAACAAATTTCATTTATCCTGAGC
AAATTTTATCTCATCACTCATCACATCCAAAGACCTTGGTTTGTAGTCCCGCATTATTAAAACGTCTTATTGACCATGAA
GGCTATAATGAGTATCAGGTGGTATTTTCTTCGGGTGGCCCATTAGCGATTGAAGGCGCGGATCAAAGTGAGGCTAAATT
ATCATCACGACCATTTGAAGTATATGGCAGTACAGAAACGGGTGGGGTGGGTTATCGCCAGCAAACCGAACCCTGTTCAT
TGTGGACGTTTTTTCCGTCTCATCAAGCGCGCTTGAATATTGAGCATTGTTTGGAATTATTGTCTCCGTTCATTGATGAC
GCACTATGGTACCCAACGAGCGATTATTGTGATTTGCTTGAAAATGGTCAATTTATGTTGAAAGGCCGAGTTGATCGAGT
CATTAAAATTGAAGAAAAGCGTCTTTCATTAACGGAAGTTGAGAAACGTTTAACACAATTAATATGGATAGATGACGCCG
CCGTTATTTCGATGGAAGAAGAAAAGCGCACTGTTTTAGGTGCTGTTATTGTGCTTTCTGACGCTGGGAACGAATTAATG
CAACAGGGGTCAAAAGGGAAGTTTTGGCTCAAATTACGCTCTGAACTGAGAGAATGGTTAGAACCAGTAGGGATCCCTAG
ACGATTCAGAGCGGTAGATGAAGTTCCATTAAATAGTCAAGGTAAGCGCCAAATGCGTGATTTACAGCAGCTGTTTAATG
ACATTAATGATTAA

Upstream 100 bases:

>100_bases
AAGTGTGGATGCTGTATAACGGCTTTATTAGTTATTTGCTTATTGGAACATTATTTGTGAGTGAGTGGTTAATTCGCCAA
TGGATAAAGAGAAAAGAAGC

Downstream 100 bases:

>100_bases
AAGAAGTACTCATGACAAAACGTAAGCCGACAATTCTGTCCACCGAAGTCGTCATTCAAGATGATGCGCATTCAAGTGTC
GTTATTACGATGAAAATAGA

Product: hypothetical protein

Products: NA

Alternate protein names: 4CL; 4-coumaroyl-CoA synthase [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MTYTSLSHLTLQSNASHSVCFDEQHNVTGYEKFQQDVQIFSAYLSDKPQRKWALCFENSYAFSVAFMALAHAGKHLILPG
NHQPAALTELAQHFDALLHDDIIETMPCEIHINYNAVDLSIQHTEKLSPLSLCDIPLTLFTSGSTDTPKPINKTLQLLDN
EIQQLELLWGEKVKDSECYSTVSHQHIYGLLFRVLWPLCSGRAFSQTNFIYPEQILSHHSSHPKTLVCSPALLKRLIDHE
GYNEYQVVFSSGGPLAIEGADQSEAKLSSRPFEVYGSTETGGVGYRQQTEPCSLWTFFPSHQARLNIEHCLELLSPFIDD
ALWYPTSDYCDLLENGQFMLKGRVDRVIKIEEKRLSLTEVEKRLTQLIWIDDAAVISMEEEKRTVLGAVIVLSDAGNELM
QQGSKGKFWLKLRSELREWLEPVGIPRRFRAVDEVPLNSQGKRQMRDLQQLFNDIND

Sequences:

>Translated_457_residues
MTYTSLSHLTLQSNASHSVCFDEQHNVTGYEKFQQDVQIFSAYLSDKPQRKWALCFENSYAFSVAFMALAHAGKHLILPG
NHQPAALTELAQHFDALLHDDIIETMPCEIHINYNAVDLSIQHTEKLSPLSLCDIPLTLFTSGSTDTPKPINKTLQLLDN
EIQQLELLWGEKVKDSECYSTVSHQHIYGLLFRVLWPLCSGRAFSQTNFIYPEQILSHHSSHPKTLVCSPALLKRLIDHE
GYNEYQVVFSSGGPLAIEGADQSEAKLSSRPFEVYGSTETGGVGYRQQTEPCSLWTFFPSHQARLNIEHCLELLSPFIDD
ALWYPTSDYCDLLENGQFMLKGRVDRVIKIEEKRLSLTEVEKRLTQLIWIDDAAVISMEEEKRTVLGAVIVLSDAGNELM
QQGSKGKFWLKLRSELREWLEPVGIPRRFRAVDEVPLNSQGKRQMRDLQQLFNDIND
>Mature_456_residues
TYTSLSHLTLQSNASHSVCFDEQHNVTGYEKFQQDVQIFSAYLSDKPQRKWALCFENSYAFSVAFMALAHAGKHLILPGN
HQPAALTELAQHFDALLHDDIIETMPCEIHINYNAVDLSIQHTEKLSPLSLCDIPLTLFTSGSTDTPKPINKTLQLLDNE
IQQLELLWGEKVKDSECYSTVSHQHIYGLLFRVLWPLCSGRAFSQTNFIYPEQILSHHSSHPKTLVCSPALLKRLIDHEG
YNEYQVVFSSGGPLAIEGADQSEAKLSSRPFEVYGSTETGGVGYRQQTEPCSLWTFFPSHQARLNIEHCLELLSPFIDDA
LWYPTSDYCDLLENGQFMLKGRVDRVIKIEEKRLSLTEVEKRLTQLIWIDDAAVISMEEEKRTVLGAVIVLSDAGNELMQ
QGSKGKFWLKLRSELREWLEPVGIPRRFRAVDEVPLNSQGKRQMRDLQQLFNDIND

Specific function: Converts p-coumaric acid into p-coumaryl CoA. This is necessary for the activation of the photoactive yellow protein (PYP) chromophore [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012743
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.12 [H]

Molecular weight: Translated: 52017; Mature: 51885

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYTSLSHLTLQSNASHSVCFDEQHNVTGYEKFQQDVQIFSAYLSDKPQRKWALCFENSY
CCCCCCEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCH
AFSVAFMALAHAGKHLILPGNHQPAALTELAQHFDALLHDDIIETMPCEIHINYNAVDLS
HHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEEEEEE
IQHTEKLSPLSLCDIPLTLFTSGSTDTPKPINKTLQLLDNEIQQLELLWGEKVKDSECYS
EECCCCCCCCEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
TVSHQHIYGLLFRVLWPLCSGRAFSQTNFIYPEQILSHHSSHPKTLVCSPALLKRLIDHE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCEEEECHHHHHHHHHCC
GYNEYQVVFSSGGPLAIEGADQSEAKLSSRPFEVYGSTETGGVGYRQQTEPCSLWTFFPS
CCCCEEEEEECCCCEEEECCCCHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEEECCC
HQARLNIEHCLELLSPFIDDALWYPTSDYCDLLENGQFMLKGRVDRVIKIEEKRLSLTEV
CCCEECHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCCEEEEHHHHCCHHHH
EKRLTQLIWIDDAAVISMEEEKRTVLGAVIVLSDAGNELMQQGSKGKFWLKLRSELREWL
HHHHHHEEEECCCEEEEEHHHHHHEEEEEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHH
EPVGIPRRFRAVDEVPLNSQGKRQMRDLQQLFNDIND
HHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TYTSLSHLTLQSNASHSVCFDEQHNVTGYEKFQQDVQIFSAYLSDKPQRKWALCFENSY
CCCCCEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCH
AFSVAFMALAHAGKHLILPGNHQPAALTELAQHFDALLHDDIIETMPCEIHINYNAVDLS
HHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEEEEEE
IQHTEKLSPLSLCDIPLTLFTSGSTDTPKPINKTLQLLDNEIQQLELLWGEKVKDSECYS
EECCCCCCCCEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
TVSHQHIYGLLFRVLWPLCSGRAFSQTNFIYPEQILSHHSSHPKTLVCSPALLKRLIDHE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCEEEECHHHHHHHHHCC
GYNEYQVVFSSGGPLAIEGADQSEAKLSSRPFEVYGSTETGGVGYRQQTEPCSLWTFFPS
CCCCEEEEEECCCCEEEECCCCHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEEECCC
HQARLNIEHCLELLSPFIDDALWYPTSDYCDLLENGQFMLKGRVDRVIKIEEKRLSLTEV
CCCEECHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCCEEEEHHHHCCHHHH
EKRLTQLIWIDDAAVISMEEEKRTVLGAVIVLSDAGNELMQQGSKGKFWLKLRSELREWL
HHHHHHEEEECCCEEEEEHHHHHHEEEEEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHH
EPVGIPRRFRAVDEVPLNSQGKRQMRDLQQLFNDIND
HHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9630474 [H]