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The map label for this gene is purR

Identifier: 209695442

GI number: 209695442

Start: 2124662

End: 2125663

Strand: Reverse

Name: purR

Synonym: VSAL_I1983

Alternate gene names: 209695442

Gene position: 2125663-2124662 (Counterclockwise)

Preceding gene: 209695443

Following gene: 209695441

Centisome position: 63.93

GC content: 38.62

Gene sequence:

>1002_bases
ATGGCTACCATTAAAGACGTTGCGAAACTTGCTTCTGTTTCGACAACCACCGTTTCTCATGTAATTAACAAAACTCGATT
TGTTGCAGAAGCAACTCAAAAAAGAGTATGGGAAGCCGTTGAAGAACTAAACTATGCACCGAGTGCCGTTGCTCGTAGTT
TAAAGTGCAACACAACTCGAACTATCGGCATGCTAGTTACCCAGTCCTTTAATCCATTTTTTGCCGAAATGATGCACGGT
GTTGAGAACTACTGTTACAAGCAAGGTTACACATTGTTTATGTGTAATACCGAAGGTGATCTAGACAAACAAAAGCACTA
CCTTCGTATGCTGGCAGAAAAACGTGTCGATGGCTTATTAGTTATGTGTTCTGATCTAAATGAACAGTTATTAGCACAAC
TTGAAAAAAACACAGAATTACCAATGGTAATCATGGACTGGGGCCCAGATAGTCCTCATACCGACAAAATTATTGATAAT
TCTGAAGAAGGTGGTTATTTAGCAACAAAACACCTGATTGAAAATGGACACACAAAAATTGGCTGTGTTACCGGTCAGCT
TGATAAAGCAACCTGTAAAGAACGAATTCATGGTTTCTACAGAGCCTTATCTGAAGCAAATTTAACGTCAAATCCAGATT
GGATATTTGAAGGTGATTTTGAATGTTCTTCTGCCTCTAAATCTGTTGAAAAAATGCTACAGATGGAAGATAAACCAACT
GCATTATTTTGTTTTAACGACATTATGGCATTAGCGGCTATCAGCAAAATTCAACAAACTGGCTTACGCGTACCTGAAGA
TATCTCAATCATTGGTTACGATAACATTGAACTTTCTGCATATTTCTCTCCACCATTGACGACGATTCACCAACCTAAAC
GTCGCGTTGGTAAGACCGCCGTTGAAATATTGTTGGAACGAATTAAAGATAAGCACCATGAAAGACGCATTTTTGAAATG
CATCCAGAAGTGGTTACTCGGAAAAGTGTCCATAAGTTGTAA

Upstream 100 bases:

>100_bases
GGCCTATTTACCTTCCAACAATCTTTATTAAAAAATAAAGAGAGACTTTGACACTATTGTTGGAACATAAATAGCCCCTT
AATAAATTAAGGCTTTTAAT

Downstream 100 bases:

>100_bases
CAATGAGTGATGTATTAATCATAAATATGCAAGTGAAAAAATATTTATTTATTTAAAACTTGAACTGGCATAACAGAAAA
TCACTCCTATTTTCTGAACC

Product: HTH-type transcriptional repressor PurR (purine nucleotide synthesis repressor)

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MATIKDVAKLASVSTTTVSHVINKTRFVAEATQKRVWEAVEELNYAPSAVARSLKCNTTRTIGMLVTQSFNPFFAEMMHG
VENYCYKQGYTLFMCNTEGDLDKQKHYLRMLAEKRVDGLLVMCSDLNEQLLAQLEKNTELPMVIMDWGPDSPHTDKIIDN
SEEGGYLATKHLIENGHTKIGCVTGQLDKATCKERIHGFYRALSEANLTSNPDWIFEGDFECSSASKSVEKMLQMEDKPT
ALFCFNDIMALAAISKIQQTGLRVPEDISIIGYDNIELSAYFSPPLTTIHQPKRRVGKTAVEILLERIKDKHHERRIFEM
HPEVVTRKSVHKL

Sequences:

>Translated_333_residues
MATIKDVAKLASVSTTTVSHVINKTRFVAEATQKRVWEAVEELNYAPSAVARSLKCNTTRTIGMLVTQSFNPFFAEMMHG
VENYCYKQGYTLFMCNTEGDLDKQKHYLRMLAEKRVDGLLVMCSDLNEQLLAQLEKNTELPMVIMDWGPDSPHTDKIIDN
SEEGGYLATKHLIENGHTKIGCVTGQLDKATCKERIHGFYRALSEANLTSNPDWIFEGDFECSSASKSVEKMLQMEDKPT
ALFCFNDIMALAAISKIQQTGLRVPEDISIIGYDNIELSAYFSPPLTTIHQPKRRVGKTAVEILLERIKDKHHERRIFEM
HPEVVTRKSVHKL
>Mature_332_residues
ATIKDVAKLASVSTTTVSHVINKTRFVAEATQKRVWEAVEELNYAPSAVARSLKCNTTRTIGMLVTQSFNPFFAEMMHGV
ENYCYKQGYTLFMCNTEGDLDKQKHYLRMLAEKRVDGLLVMCSDLNEQLLAQLEKNTELPMVIMDWGPDSPHTDKIIDNS
EEGGYLATKHLIENGHTKIGCVTGQLDKATCKERIHGFYRALSEANLTSNPDWIFEGDFECSSASKSVEKMLQMEDKPTA
LFCFNDIMALAAISKIQQTGLRVPEDISIIGYDNIELSAYFSPPLTTIHQPKRRVGKTAVEILLERIKDKHHERRIFEMH
PEVVTRKSVHKL

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787948, Length=331, Percent_Identity=52.8700906344411, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1790194, Length=329, Percent_Identity=46.2006079027356, Blast_Score=324, Evalue=6e-90,
Organism=Escherichia coli, GI1790369, Length=334, Percent_Identity=35.3293413173653, Blast_Score=228, Evalue=3e-61,
Organism=Escherichia coli, GI1789202, Length=293, Percent_Identity=35.1535836177474, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1788474, Length=330, Percent_Identity=30.3030303030303, Blast_Score=164, Evalue=6e-42,
Organism=Escherichia coli, GI1789068, Length=294, Percent_Identity=27.891156462585, Blast_Score=151, Evalue=5e-38,
Organism=Escherichia coli, GI1786540, Length=305, Percent_Identity=31.4754098360656, Blast_Score=151, Evalue=6e-38,
Organism=Escherichia coli, GI1787580, Length=321, Percent_Identity=26.1682242990654, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1787906, Length=326, Percent_Identity=24.5398773006135, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI48994940, Length=313, Percent_Identity=27.4760383386581, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789456, Length=324, Percent_Identity=29.0123456790123, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1790715, Length=329, Percent_Identity=24.9240121580547, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1786268, Length=305, Percent_Identity=24.2622950819672, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1790689, Length=325, Percent_Identity=22.1538461538462, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURR_ALISL (B6EH86)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263371.1
- ProteinModelPortal:   B6EH86
- GeneID:   6989279
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I1983
- HOGENOM:   HBG753640
- OMA:   CFNDIMA
- ProtClustDB:   CLSK874518
- GO:   GO:0005622
- HAMAP:   MF_01277
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761
- PRINTS:   PR00036
- SMART:   SM00354

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1; SSF47413 Lambda_like_DNA

EC number: NA

Molecular weight: Translated: 37707; Mature: 37576

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00356 HTH_LACI_1; PS50932 HTH_LACI_2

Important sites: BINDING 73-73 BINDING 189-189 BINDING 191-191 BINDING 220-220 BINDING 274-274

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVAKLASVSTTTVSHVINKTRFVAEATQKRVWEAVEELNYAPSAVARSLKCNTTR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCH
TIGMLVTQSFNPFFAEMMHGVENYCYKQGYTLFMCNTEGDLDKQKHYLRMLAEKRVDGLL
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEE
VMCSDLNEQLLAQLEKNTELPMVIMDWGPDSPHTDKIIDNSEEGGYLATKHLIENGHTKI
EEHHHCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEE
GCVTGQLDKATCKERIHGFYRALSEANLTSNPDWIFEGDFECSSASKSVEKMLQMEDKPT
EEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCC
ALFCFNDIMALAAISKIQQTGLRVPEDISIIGYDNIELSAYFSPPLTTIHQPKRRVGKTA
EEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEEEEECCCCHHHHCHHHHHHHHH
VEILLERIKDKHHERRIFEMHPEVVTRKSVHKL
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCC
>Mature Secondary Structure 
ATIKDVAKLASVSTTTVSHVINKTRFVAEATQKRVWEAVEELNYAPSAVARSLKCNTTR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCH
TIGMLVTQSFNPFFAEMMHGVENYCYKQGYTLFMCNTEGDLDKQKHYLRMLAEKRVDGLL
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEE
VMCSDLNEQLLAQLEKNTELPMVIMDWGPDSPHTDKIIDNSEEGGYLATKHLIENGHTKI
EEHHHCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEE
GCVTGQLDKATCKERIHGFYRALSEANLTSNPDWIFEGDFECSSASKSVEKMLQMEDKPT
EEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCC
ALFCFNDIMALAAISKIQQTGLRVPEDISIIGYDNIELSAYFSPPLTTIHQPKRRVGKTA
EEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEEEEECCCCHHHHCHHHHHHHHH
VEILLERIKDKHHERRIFEMHPEVVTRKSVHKL
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA