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The map label for this gene is ybaZ [H]

Identifier: 209695429

GI number: 209695429

Start: 2112749

End: 2113051

Strand: Reverse

Name: ybaZ [H]

Synonym: VSAL_I1970

Alternate gene names: 209695429

Gene position: 2113051-2112749 (Counterclockwise)

Preceding gene: 209695431

Following gene: 209695427

Centisome position: 63.55

GC content: 36.96

Gene sequence:

>303_bases
ATGAATGACTTTGTTCAAAGTATTTACACGAACCTTCTTCATGTCCCAAAAGGACAAGTGATAACCTATGGGCAACTTGC
CAAACTTGCAGGGTACCCAAACCATTCAAGACAGGTAGGTAAATTACTCGCAAAACTGCCTAACGATACAAAACTGGCTT
GGTATCGAGTGATCAATTCACAAGGAAAAATATCATTGACTGGAGAACGTTTTTTCGTACAAAAAGAGAAATTAGAAGAG
GAAGGGATTAGCATTAAAGACGATGGAAGAATTGAACATTTTAAGAAAGTAATAATGCGCTAA

Upstream 100 bases:

>100_bases
TTTAGCTTAAGGGTGTTACCCTTGTTATCGTTTTAGTTTAGCGGCAATATAGGGTTTTATCACTGATTCACCTCACTTTA
ATTAAAAGGTTGTTACGTCA

Downstream 100 bases:

>100_bases
TCTAAAGCACATTATTACTTCATATCACTTATAATAACGACTTACTGACGTACACCTTTTAGCAATAGGTCTTGAGTCAT
TGTTTTTTCTTGGTCAGTAA

Product: methylated-DNA methyltransferase

Products: DNA; protein S-methyl-L-cysteine

Alternate protein names: NA

Number of amino acids: Translated: 100; Mature: 100

Protein sequence:

>100_residues
MNDFVQSIYTNLLHVPKGQVITYGQLAKLAGYPNHSRQVGKLLAKLPNDTKLAWYRVINSQGKISLTGERFFVQKEKLEE
EGISIKDDGRIEHFKKVIMR

Sequences:

>Translated_100_residues
MNDFVQSIYTNLLHVPKGQVITYGQLAKLAGYPNHSRQVGKLLAKLPNDTKLAWYRVINSQGKISLTGERFFVQKEKLEE
EGISIKDDGRIEHFKKVIMR
>Mature_100_residues
MNDFVQSIYTNLLHVPKGQVITYGQLAKLAGYPNHSRQVGKLLAKLPNDTKLAWYRVINSQGKISLTGERFFVQKEKLEE
EGISIKDDGRIEHFKKVIMR

Specific function: Unknown

COG id: COG3695

COG function: function code L; Predicted methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Escherichia coli, GI1786659, Length=91, Percent_Identity=41.7582417582418, Blast_Score=84, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014048
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1 [H]

EC number: 2.1.1.63

Molecular weight: Translated: 11488; Mature: 11488

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDFVQSIYTNLLHVPKGQVITYGQLAKLAGYPNHSRQVGKLLAKLPNDTKLAWYRVINS
CCHHHHHHHHHHHCCCCCCEEEHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHEEECC
QGKISLTGERFFVQKEKLEEEGISIKDDGRIEHFKKVIMR
CCCEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MNDFVQSIYTNLLHVPKGQVITYGQLAKLAGYPNHSRQVGKLLAKLPNDTKLAWYRVINS
CCHHHHHHHHHHHCCCCCCEEEHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHEEECC
QGKISLTGERFFVQKEKLEEEGISIKDDGRIEHFKKVIMR
CCCEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA containing 6-O-methylguanine; protein L-cysteine

Specific reaction: DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]