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The map label for this gene is cls [H]

Identifier: 209695397

GI number: 209695397

Start: 2068438

End: 2069892

Strand: Reverse

Name: cls [H]

Synonym: VSAL_I1934

Alternate gene names: 209695397

Gene position: 2069892-2068438 (Counterclockwise)

Preceding gene: 209695399

Following gene: 209695395

Centisome position: 62.25

GC content: 39.38

Gene sequence:

>1455_bases
ATGGATAAGTTCTACCAAGCACTCACTATCGTTAGCTTTATCTTGTATTGGCTTTTAGTCGCCGGGGTAACGATTCGTGT
CGTCTTTAAACCAAGGCCTGTAGGCGTCTCTCTTGCTTGGTTAATGGTTATTTATATCCTTCCCGTTATTGGTGTAATGG
CTTATTTTTTAATTGGTGAGCTTAATTTAGGCCGTACTCGTGGTGAACGAGCAAAAGCAATGTTTACTCCTTATGAACAG
TGGTTTGCTCAAGTTCATCACTGTCAGTCTCATCAGCCTCATTCTATTTCACATCGAATTTCATCCATTCATAATTTGTG
TTCAAATCGATTAGGTATTCCAGCATTAAGTGGCGACACCCTTTCTTTATTAAATACCCCGTCAGAAATTCTGACCGCCA
TTATTCGAGATATTGAAAAAGCAGAACATGAAATCAATATGGAATTCTACATTTGGCATCATGGTGGATTAGCCGATGGT
GTCGCAAGCGCATTAATTCAAGCGGCTAAACGAGGCGTAACCGTTAATGTTTTACTGGATTCGGCTGGGAGTATTGATTT
TTTCAAAAGCCACAGGCCAAGAATGTTACGAGAATCTGGGGTCAATATTGTTGAAGCCTTAAGCGTGAGTCCATTTCGTA
TGTTCTTTAGACGACTTGACCTACGATTACATCGAAAAATTGTCGTTATCGATAATAAAATTGCCTATACAGGATCAATG
AATTTAGTCGATCCTCAATATTTCAAACAAAATTCGGGCGTAGGCCAATGGGTTGACGTAATGGTTCGTATTACCGGCCC
TAATGTAGCGATCTTAAATACGATTTATGCATGGGATTGGGAAGTAGAAACGGGAATACGAGCACTGCCAACAATACCTG
AATGCCCATTTGAAATAGACCCATTTAACCACACTACTCAGGTAGTGCCTTCGGGTCCGGGTATGCCAGATGACATTATT
CAGCAAGTATTGATCACCGCAATTAATCAAGCTAGAGAATCACTCACAATCACAACACCTTATTTTGTTCCTAGTGAGTT
ATTGCTCCATACTTTAAGAACCACAGCTCACCGAGGAGTAAAAGTTACGTTAATTTTACCCAAGAAGAACGATTCATTAA
TGGTAAGTTGGGCTTCAAAATCATTCTTTGAAACGCTTCTAAACGCAGGGGTTCATATCTTTGAGTTTAAAGGCGGGTTA
TTGCATACTAAATCAGTGGTCATTGATAATAACTATTGCATTATTGGTACCGTGAATTTAGATATGCGCAGCTTCTGGTT
GAATTTTGAAGTCCGTTTGATTGTTGATGATCCTGAATTTACAAAACAATTAACGTGGGTATTAGATGGCTACCTAGAAC
ATTCTGATGCGATTGTTTTAGAAAATTGGCAGCAACGAAATTGGGCTCATCGATTTACTGAGCGTTTCTTCTCTATGTTT
AGCCCCTTACTTTAA

Upstream 100 bases:

>100_bases
ATCAAACCTCTCTTATTTCGAATTTTTATCCCCTTTACCTACTCTTTTGCCTCAAGTCCTGATATTAATACATATTCTCT
CTATATATATGAAATCACGA

Downstream 100 bases:

>100_bases
AACAAAGCATAAATTAAAAAGCCCACGATAATTTAATTATCGTGGGCTTTTTTAACATCCGTAAATATTAACGAATGCCA
CTTAAAAATTCATGTCTTGT

Product: cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MDKFYQALTIVSFILYWLLVAGVTIRVVFKPRPVGVSLAWLMVIYILPVIGVMAYFLIGELNLGRTRGERAKAMFTPYEQ
WFAQVHHCQSHQPHSISHRISSIHNLCSNRLGIPALSGDTLSLLNTPSEILTAIIRDIEKAEHEINMEFYIWHHGGLADG
VASALIQAAKRGVTVNVLLDSAGSIDFFKSHRPRMLRESGVNIVEALSVSPFRMFFRRLDLRLHRKIVVIDNKIAYTGSM
NLVDPQYFKQNSGVGQWVDVMVRITGPNVAILNTIYAWDWEVETGIRALPTIPECPFEIDPFNHTTQVVPSGPGMPDDII
QQVLITAINQARESLTITTPYFVPSELLLHTLRTTAHRGVKVTLILPKKNDSLMVSWASKSFFETLLNAGVHIFEFKGGL
LHTKSVVIDNNYCIIGTVNLDMRSFWLNFEVRLIVDDPEFTKQLTWVLDGYLEHSDAIVLENWQQRNWAHRFTERFFSMF
SPLL

Sequences:

>Translated_484_residues
MDKFYQALTIVSFILYWLLVAGVTIRVVFKPRPVGVSLAWLMVIYILPVIGVMAYFLIGELNLGRTRGERAKAMFTPYEQ
WFAQVHHCQSHQPHSISHRISSIHNLCSNRLGIPALSGDTLSLLNTPSEILTAIIRDIEKAEHEINMEFYIWHHGGLADG
VASALIQAAKRGVTVNVLLDSAGSIDFFKSHRPRMLRESGVNIVEALSVSPFRMFFRRLDLRLHRKIVVIDNKIAYTGSM
NLVDPQYFKQNSGVGQWVDVMVRITGPNVAILNTIYAWDWEVETGIRALPTIPECPFEIDPFNHTTQVVPSGPGMPDDII
QQVLITAINQARESLTITTPYFVPSELLLHTLRTTAHRGVKVTLILPKKNDSLMVSWASKSFFETLLNAGVHIFEFKGGL
LHTKSVVIDNNYCIIGTVNLDMRSFWLNFEVRLIVDDPEFTKQLTWVLDGYLEHSDAIVLENWQQRNWAHRFTERFFSMF
SPLL
>Mature_484_residues
MDKFYQALTIVSFILYWLLVAGVTIRVVFKPRPVGVSLAWLMVIYILPVIGVMAYFLIGELNLGRTRGERAKAMFTPYEQ
WFAQVHHCQSHQPHSISHRISSIHNLCSNRLGIPALSGDTLSLLNTPSEILTAIIRDIEKAEHEINMEFYIWHHGGLADG
VASALIQAAKRGVTVNVLLDSAGSIDFFKSHRPRMLRESGVNIVEALSVSPFRMFFRRLDLRLHRKIVVIDNKIAYTGSM
NLVDPQYFKQNSGVGQWVDVMVRITGPNVAILNTIYAWDWEVETGIRALPTIPECPFEIDPFNHTTQVVPSGPGMPDDII
QQVLITAINQARESLTITTPYFVPSELLLHTLRTTAHRGVKVTLILPKKNDSLMVSWASKSFFETLLNAGVHIFEFKGGL
LHTKSVVIDNNYCIIGTVNLDMRSFWLNFEVRLIVDDPEFTKQLTWVLDGYLEHSDAIVLENWQQRNWAHRFTERFFSMF
SPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=487, Percent_Identity=49.0759753593429, Blast_Score=509, Evalue=1e-145,
Organism=Escherichia coli, GI1787007, Length=347, Percent_Identity=29.6829971181556, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 55044; Mature: 55044

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKFYQALTIVSFILYWLLVAGVTIRVVFKPRPVGVSLAWLMVIYILPVIGVMAYFLIGE
CCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
LNLGRTRGERAKAMFTPYEQWFAQVHHCQSHQPHSISHRISSIHNLCSNRLGIPALSGDT
CCCCCCCCCCHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
LSLLNTPSEILTAIIRDIEKAEHEINMEFYIWHHGGLADGVASALIQAAKRGVTVNVLLD
CHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEEC
SAGSIDFFKSHRPRMLRESGVNIVEALSVSPFRMFFRRLDLRLHRKIVVIDNKIAYTGSM
CCCCCHHHHCCCCHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHCCEEEEEECEEEEECCC
NLVDPQYFKQNSGVGQWVDVMVRITGPNVAILNTIYAWDWEVETGIRALPTIPECPFEID
CCCCHHHHHCCCCCCCEEEEEEEECCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCCEEC
PFNHTTQVVPSGPGMPDDIIQQVLITAINQARESLTITTPYFVPSELLLHTLRTTAHRGV
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHHHHHHCCCE
KVTLILPKKNDSLMVSWASKSFFETLLNAGVHIFEFKGGLLHTKSVVIDNNYCIIGTVNL
EEEEEEECCCCCEEEEEHHHHHHHHHHHCCEEEEEECCCEEEEEEEEEECCEEEEEEEEC
DMRSFWLNFEVRLIVDDPEFTKQLTWVLDGYLEHSDAIVLENWQQRNWAHRFTERFFSMF
CCEEEEEEEEEEEEECCHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHH
SPLL
CCCC
>Mature Secondary Structure
MDKFYQALTIVSFILYWLLVAGVTIRVVFKPRPVGVSLAWLMVIYILPVIGVMAYFLIGE
CCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
LNLGRTRGERAKAMFTPYEQWFAQVHHCQSHQPHSISHRISSIHNLCSNRLGIPALSGDT
CCCCCCCCCCHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
LSLLNTPSEILTAIIRDIEKAEHEINMEFYIWHHGGLADGVASALIQAAKRGVTVNVLLD
CHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEEC
SAGSIDFFKSHRPRMLRESGVNIVEALSVSPFRMFFRRLDLRLHRKIVVIDNKIAYTGSM
CCCCCHHHHCCCCHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHCCEEEEEECEEEEECCC
NLVDPQYFKQNSGVGQWVDVMVRITGPNVAILNTIYAWDWEVETGIRALPTIPECPFEID
CCCCHHHHHCCCCCCCEEEEEEEECCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCCEEC
PFNHTTQVVPSGPGMPDDIIQQVLITAINQARESLTITTPYFVPSELLLHTLRTTAHRGV
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHHHHHHCCCE
KVTLILPKKNDSLMVSWASKSFFETLLNAGVHIFEFKGGLLHTKSVVIDNNYCIIGTVNL
EEEEEEECCCCCEEEEEHHHHHHHHHHHCCEEEEEECCCEEEEEEEEEECCEEEEEEEEC
DMRSFWLNFEVRLIVDDPEFTKQLTWVLDGYLEHSDAIVLENWQQRNWAHRFTERFFSMF
CCEEEEEEEEEEEEECCHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHH
SPLL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA