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The map label for this gene is hsdM [C]

Identifier: 209695268

GI number: 209695268

Start: 1912607

End: 1914370

Strand: Reverse

Name: hsdM [C]

Synonym: VSAL_I1792

Alternate gene names: 209695268

Gene position: 1914370-1912607 (Counterclockwise)

Preceding gene: 209695270

Following gene: 209695267

Centisome position: 57.57

GC content: 43.54

Gene sequence:

>1764_bases
ATGACTAACAAGCTTTCTCTTCAACAGCTCGAATCCTTCTTATGGGAAACCGCCGACATTCTTCGTGGCAATATGGATGC
GTCTGAATTTAAAGATTACATCTTCGGCATGATGTTTTTAAAACGTCTATCGGATGCCTTTGAAGAATCTCAAGAAAAAG
TTATTCAATACTACCTAGACAAAGGCAAAACGCAGGCTCAAGCCGAAGAGTTAGCGAATGATGAAGACGAATACGACAGC
ACCTTTTTTATCCCAGAAAACGCCCGTTGGTCAGTGCTTAAAGATTTAAAGCACAACATTGGTGAGCAGCTAAATAAAGC
GACAGAATCGATTGAAGAGTTTAACTCGGCGCTAGAAGGCGTATTAGTGACGATTGACTTCAACATCAAAAACAAACTGT
CAGATAAAAAGCTGCAAGATTTACTGTCGCATTTCAATAAATACCGCTTACGTAATGAGGACTTTGATCGCCCTGATTTA
CTGGGTACGGCTTACGAATACTTGATTAAAATGTTTGCCGACAGCGCAGGCAAAAAAGGCGGCGAGTTTTATACCCCAAG
TGAAGTGGTTCAGCTATTGGTTGAGCTATTAAAGCCCCATGCAGGAATGCGTATTTACGATCCAACATCAGGGTCGGGCG
GTATGCTTGTTCAAACGCGTAATCAATTAGAAAAGCAGGGTGAAAACGCCGCTAACTTGTCACTGTATGGTCAAGAGATG
AACTTGAACACATGGGCAATTTGTAAGATGAACATGTTCTTACATGGCGTACAAAATGCCGATATTCGTAAAGGCGATAC
ACTGCGTGACCCACAGCACACCGAAGGCGGCGAGCTAATGAGCTTTGACCGCGTTATTGCCAACCCACCGTTCTCATTGA
AAAAGTGGGGCAAAGACGAGTGTGATAACGACGGTTTTGGACGTTTTCCTTACGGCACACCACCAAAAGACGCGGGTGAT
TTAGCCTTTGTACAACACATGATCGCCAGCACCAACAGCGAAGGCATGGTAGGCGTGGTTATGCCTCATGGGGTGTTATT
CCGTGGCTCAAGTGAAAAAGCGATTCGCCAAGGGATTTTAGAAGACGATTTATTAGAAGCCGTGGTGGGTTTACCATCAG
GATTATTCTACGGCACAGGCATTCCTGCGTGTTTGCTTATCATCAATAAGAACAAACCAAGCGCACGTAAAGGCAAGGTG
TTGTTCATTAATGGTGAATTGGAATTTGCAGAGGGCAAAAACCAAAACAAATTGCGCCCAGAAGACATTGCTAAAATTGT
AACCACCTTTGAAAACCACTCGTTTGAAAGCCAGTGTGACATTAAGCGTTACGCACGTGTTGTTCCATTATCAGAAATTG
CTGAGAACGATTTTAACCTTAACATCCGCCGTTATGCCGATACGTCACCACCGGCGGAGATCTTTGATGTACGCGCAATC
CTTCACGGTGGCATTCCAGTGCGTGAAGTGAATGATAATTACATTCAAGAAGAGATGTTAAACGGCTTTGATGTGTCGGC
CGTCTTTGATATAAAAAGTGATAAAAAAGACAACGACTATTATACATTCAAACCAAGCATTGAAAGCAAAGAACAGATCC
GTCTGGTTGTTGAAGAACAAGTGGGCGATGTTGATGCGAAGATCATCAGCCAACTAGAACACTGGTGGGACAAATACCAA
GTATCACTGCATGAGTTAGATGCACAAGTGACCGAAGCTGAACAAGTGATGCAAGGTTACTTGAAGGAGTTGGGTTATGA
GTGA

Upstream 100 bases:

>100_bases
CCCAACCCCATAACCTCGTAAAATCCACCACATATCAATTCGTTTGAATAAACCCGTACAATTGAGTACGAGTTGCCCCT
TATTTAATAGGCACAATTGC

Downstream 100 bases:

>100_bases
GTTTGTGCCTGAAGGTTGGATTTCTAAACAGTTAATCGACTGTAGTGTTGATGGTATTAGCAATGGTGTTTTTTGTGATC
CGAATAAAGTTGGTAGTGGA

Product: N-6 adenine-specific DNA methylase

Products: NA

Alternate protein names: M.MjaXP [H]

Number of amino acids: Translated: 587; Mature: 586

Protein sequence:

>587_residues
MTNKLSLQQLESFLWETADILRGNMDASEFKDYIFGMMFLKRLSDAFEESQEKVIQYYLDKGKTQAQAEELANDEDEYDS
TFFIPENARWSVLKDLKHNIGEQLNKATESIEEFNSALEGVLVTIDFNIKNKLSDKKLQDLLSHFNKYRLRNEDFDRPDL
LGTAYEYLIKMFADSAGKKGGEFYTPSEVVQLLVELLKPHAGMRIYDPTSGSGGMLVQTRNQLEKQGENAANLSLYGQEM
NLNTWAICKMNMFLHGVQNADIRKGDTLRDPQHTEGGELMSFDRVIANPPFSLKKWGKDECDNDGFGRFPYGTPPKDAGD
LAFVQHMIASTNSEGMVGVVMPHGVLFRGSSEKAIRQGILEDDLLEAVVGLPSGLFYGTGIPACLLIINKNKPSARKGKV
LFINGELEFAEGKNQNKLRPEDIAKIVTTFENHSFESQCDIKRYARVVPLSEIAENDFNLNIRRYADTSPPAEIFDVRAI
LHGGIPVREVNDNYIQEEMLNGFDVSAVFDIKSDKKDNDYYTFKPSIESKEQIRLVVEEQVGDVDAKIISQLEHWWDKYQ
VSLHELDAQVTEAEQVMQGYLKELGYE

Sequences:

>Translated_587_residues
MTNKLSLQQLESFLWETADILRGNMDASEFKDYIFGMMFLKRLSDAFEESQEKVIQYYLDKGKTQAQAEELANDEDEYDS
TFFIPENARWSVLKDLKHNIGEQLNKATESIEEFNSALEGVLVTIDFNIKNKLSDKKLQDLLSHFNKYRLRNEDFDRPDL
LGTAYEYLIKMFADSAGKKGGEFYTPSEVVQLLVELLKPHAGMRIYDPTSGSGGMLVQTRNQLEKQGENAANLSLYGQEM
NLNTWAICKMNMFLHGVQNADIRKGDTLRDPQHTEGGELMSFDRVIANPPFSLKKWGKDECDNDGFGRFPYGTPPKDAGD
LAFVQHMIASTNSEGMVGVVMPHGVLFRGSSEKAIRQGILEDDLLEAVVGLPSGLFYGTGIPACLLIINKNKPSARKGKV
LFINGELEFAEGKNQNKLRPEDIAKIVTTFENHSFESQCDIKRYARVVPLSEIAENDFNLNIRRYADTSPPAEIFDVRAI
LHGGIPVREVNDNYIQEEMLNGFDVSAVFDIKSDKKDNDYYTFKPSIESKEQIRLVVEEQVGDVDAKIISQLEHWWDKYQ
VSLHELDAQVTEAEQVMQGYLKELGYE
>Mature_586_residues
TNKLSLQQLESFLWETADILRGNMDASEFKDYIFGMMFLKRLSDAFEESQEKVIQYYLDKGKTQAQAEELANDEDEYDST
FFIPENARWSVLKDLKHNIGEQLNKATESIEEFNSALEGVLVTIDFNIKNKLSDKKLQDLLSHFNKYRLRNEDFDRPDLL
GTAYEYLIKMFADSAGKKGGEFYTPSEVVQLLVELLKPHAGMRIYDPTSGSGGMLVQTRNQLEKQGENAANLSLYGQEMN
LNTWAICKMNMFLHGVQNADIRKGDTLRDPQHTEGGELMSFDRVIANPPFSLKKWGKDECDNDGFGRFPYGTPPKDAGDL
AFVQHMIASTNSEGMVGVVMPHGVLFRGSSEKAIRQGILEDDLLEAVVGLPSGLFYGTGIPACLLIINKNKPSARKGKVL
FINGELEFAEGKNQNKLRPEDIAKIVTTFENHSFESQCDIKRYARVVPLSEIAENDFNLNIRRYADTSPPAEIFDVRAIL
HGGIPVREVNDNYIQEEMLNGFDVSAVFDIKSDKKDNDYYTFKPSIESKEQIRLVVEEQVGDVDAKIISQLEHWWDKYQV
SLHELDAQVTEAEQVMQGYLKELGYE

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=397, Percent_Identity=23.6775818639798, Blast_Score=105, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 66589; Mature: 66458

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKLSLQQLESFLWETADILRGNMDASEFKDYIFGMMFLKRLSDAFEESQEKVIQYYLD
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGKTQAQAEELANDEDEYDSTFFIPENARWSVLKDLKHNIGEQLNKATESIEEFNSALEG
CCCHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VLVTIDFNIKNKLSDKKLQDLLSHFNKYRLRNEDFDRPDLLGTAYEYLIKMFADSAGKKG
EEEEEEECCHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
GEFYTPSEVVQLLVELLKPHAGMRIYDPTSGSGGMLVQTRNQLEKQGENAANLSLYGQEM
CCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEEEHHHHHHCCCCCCEEEEECCCC
NLNTWAICKMNMFLHGVQNADIRKGDTLRDPQHTEGGELMSFDRVIANPPFSLKKWGKDE
CCCEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHCCCCCHHHHCCCCC
CDNDGFGRFPYGTPPKDAGDLAFVQHMIASTNSEGMVGVVMPHGVLFRGSSEKAIRQGIL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCEEEECCCHHHHHHCCC
EDDLLEAVVGLPSGLFYGTGIPACLLIINKNKPSARKGKVLFINGELEFAEGKNQNKLRP
HHHHHHHHHCCCCCCHHCCCCCEEEEEEECCCCCCCCCCEEEEECEEEECCCCCCCCCCH
EDIAKIVTTFENHSFESQCDIKRYARVVPLSEIAENDFNLNIRRYADTSPPAEIFDVRAI
HHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHCCCCCEEEEECCCCCCCHHHHHHHHH
LHGGIPVREVNDNYIQEEMLNGFDVSAVFDIKSDKKDNDYYTFKPSIESKEQIRLVVEEQ
HHCCCCEEECCHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHH
VGDVDAKIISQLEHWWDKYQVSLHELDAQVTEAEQVMQGYLKELGYE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TNKLSLQQLESFLWETADILRGNMDASEFKDYIFGMMFLKRLSDAFEESQEKVIQYYLD
CCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGKTQAQAEELANDEDEYDSTFFIPENARWSVLKDLKHNIGEQLNKATESIEEFNSALEG
CCCHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VLVTIDFNIKNKLSDKKLQDLLSHFNKYRLRNEDFDRPDLLGTAYEYLIKMFADSAGKKG
EEEEEEECCHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
GEFYTPSEVVQLLVELLKPHAGMRIYDPTSGSGGMLVQTRNQLEKQGENAANLSLYGQEM
CCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEEEHHHHHHCCCCCCEEEEECCCC
NLNTWAICKMNMFLHGVQNADIRKGDTLRDPQHTEGGELMSFDRVIANPPFSLKKWGKDE
CCCEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHCCCCCHHHHCCCCC
CDNDGFGRFPYGTPPKDAGDLAFVQHMIASTNSEGMVGVVMPHGVLFRGSSEKAIRQGIL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCEEEECCCHHHHHHCCC
EDDLLEAVVGLPSGLFYGTGIPACLLIINKNKPSARKGKVLFINGELEFAEGKNQNKLRP
HHHHHHHHHCCCCCCHHCCCCCEEEEEEECCCCCCCCCCEEEEECEEEECCCCCCCCCCH
EDIAKIVTTFENHSFESQCDIKRYARVVPLSEIAENDFNLNIRRYADTSPPAEIFDVRAI
HHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHCCCCCEEEEECCCCCCCHHHHHHHHH
LHGGIPVREVNDNYIQEEMLNGFDVSAVFDIKSDKKDNDYYTFKPSIESKEQIRLVVEEQ
HHCCCCEEECCHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHH
VGDVDAKIISQLEHWWDKYQVSLHELDAQVTEAEQVMQGYLKELGYE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]