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The map label for this gene is npdA [H]

Identifier: 209695088

GI number: 209695088

Start: 1695383

End: 1696096

Strand: Reverse

Name: npdA [H]

Synonym: VSAL_I1585

Alternate gene names: 209695088

Gene position: 1696096-1695383 (Counterclockwise)

Preceding gene: 209695091

Following gene: 209695082

Centisome position: 51.01

GC content: 39.5

Gene sequence:

>714_bases
ATGCTTTTTCCATATAAGAATATTGTGATTTTAACTGGCGCTGGTATTTCCGCTGAATCTGGGATTCAAACATTTCGCGC
CAGTGATGGCTTATGGGAAAATCACCGAATTGAAGATGTAGCGACACCGGAAGGTTTTTTCAAGGATCCTGATTTGGTTC
AAGATTTCTATAATAAACGTCGAGCGTTATTAAAGAGTGATGCTATTCAACCAAATGCGGCACATAAAGCATTAGCTCGA
TTAGAAAAAGAACTCGATGGGACCGTTACTATTGTGACTCAAAATATTGATGACTTACACGAGCGTGGTGGAAGTAAAAA
TGTGATTCATATGCATGGTGAACTCAATAAGATTCGCTGTGAAGAATCGAATCAAGTGATTGCTTGTACTGATGATATAC
AGACTGGTGATTTGTGTCATTGTTGCCAGATCCCTGCGCAGTTGCGTCCTCATGTTGTTTGGTTTGGTGAAATGCCTCTT
GAAATGGGAAGAATTCACGATCTATTAAGTCAGGCAGATTTGTTTATTTCTATTGGTACATCAGGTTCTGTTTACCCTGC
TGCTGGCTTTGTTCATGAAGCCACATTACATGGTGCACATACCATTGAAATTAATCTTGAAGCAAGTGCTGTTGAAGATG
AGTTTGAAGAAAAGCGTTATGGGAAAGCGGGGGTGTTAGTGCCTGAACTTGTTTCTGAATTATTAGAAGTATAA

Upstream 100 bases:

>100_bases
GTGATAGTGATATTTTTGTGTTATGTTATATTTAACTGATTGATCTTGTTGATAGTCTGTAGAAATAATACGTGTTTTAG
AATAAATAAAGGATGATGAC

Downstream 100 bases:

>100_bases
CGTTTACTTTGTCGGTTATTTTCTCTTATTAAATAAAAAAGCCAATGAATATAATCATTGGCTTTTTGTTTTAATGATAC
ATGAACTATTACATATCAAC

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MLFPYKNIVILTGAGISAESGIQTFRASDGLWENHRIEDVATPEGFFKDPDLVQDFYNKRRALLKSDAIQPNAAHKALAR
LEKELDGTVTIVTQNIDDLHERGGSKNVIHMHGELNKIRCEESNQVIACTDDIQTGDLCHCCQIPAQLRPHVVWFGEMPL
EMGRIHDLLSQADLFISIGTSGSVYPAAGFVHEATLHGAHTIEINLEASAVEDEFEEKRYGKAGVLVPELVSELLEV

Sequences:

>Translated_237_residues
MLFPYKNIVILTGAGISAESGIQTFRASDGLWENHRIEDVATPEGFFKDPDLVQDFYNKRRALLKSDAIQPNAAHKALAR
LEKELDGTVTIVTQNIDDLHERGGSKNVIHMHGELNKIRCEESNQVIACTDDIQTGDLCHCCQIPAQLRPHVVWFGEMPL
EMGRIHDLLSQADLFISIGTSGSVYPAAGFVHEATLHGAHTIEINLEASAVEDEFEEKRYGKAGVLVPELVSELLEV
>Mature_237_residues
MLFPYKNIVILTGAGISAESGIQTFRASDGLWENHRIEDVATPEGFFKDPDLVQDFYNKRRALLKSDAIQPNAAHKALAR
LEKELDGTVTIVTQNIDDLHERGGSKNVIHMHGELNKIRCEESNQVIACTDDIQTGDLCHCCQIPAQLRPHVVWFGEMPL
EMGRIHDLLSQADLFISIGTSGSVYPAAGFVHEATLHGAHTIEINLEASAVEDEFEEKRYGKAGVLVPELVSELLEV

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=236, Percent_Identity=37.7118644067797, Blast_Score=161, Evalue=5e-40,
Organism=Homo sapiens, GI6912664, Length=254, Percent_Identity=35.8267716535433, Blast_Score=154, Evalue=5e-38,
Organism=Homo sapiens, GI13787215, Length=237, Percent_Identity=36.7088607594937, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI6912662, Length=258, Percent_Identity=26.7441860465116, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI7657575, Length=220, Percent_Identity=29.5454545454545, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI6912660, Length=210, Percent_Identity=28.0952380952381, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI308199517, Length=229, Percent_Identity=66.8122270742358, Blast_Score=323, Evalue=8e-90,
Organism=Caenorhabditis elegans, GI17541892, Length=200, Percent_Identity=30, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28571445, Length=189, Percent_Identity=30.1587301587302, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17137536, Length=225, Percent_Identity=27.1111111111111, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 26213; Mature: 26213

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFPYKNIVILTGAGISAESGIQTFRASDGLWENHRIEDVATPEGFFKDPDLVQDFYNKR
CCCCCCCEEEEECCCCCCHHCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RALLKSDAIQPNAAHKALARLEKELDGTVTIVTQNIDDLHERGGSKNVIHMHGELNKIRC
HHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHCCCCCCEEEECCCCCEEEE
EESNQVIACTDDIQTGDLCHCCQIPAQLRPHVVWFGEMPLEMGRIHDLLSQADLFISIGT
CCCCCEEEEECCCCCCCCHHHHCCCHHCCCEEEEEECCCHHHHHHHHHHHCCCEEEEECC
SGSVYPAAGFVHEATLHGAHTIEINLEASAVEDEFEEKRYGKAGVLVPELVSELLEV
CCCCCCCCCHHHHHEECCEEEEEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MLFPYKNIVILTGAGISAESGIQTFRASDGLWENHRIEDVATPEGFFKDPDLVQDFYNKR
CCCCCCCEEEEECCCCCCHHCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RALLKSDAIQPNAAHKALARLEKELDGTVTIVTQNIDDLHERGGSKNVIHMHGELNKIRC
HHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHCCCCCCEEEECCCCCEEEE
EESNQVIACTDDIQTGDLCHCCQIPAQLRPHVVWFGEMPLEMGRIHDLLSQADLFISIGT
CCCCCEEEEECCCCCCCCHHHHCCCHHCCCEEEEEECCCHHHHHHHHHHHCCCEEEEECC
SGSVYPAAGFVHEATLHGAHTIEINLEASAVEDEFEEKRYGKAGVLVPELVSELLEV
CCCCCCCCCHHHHHEECCEEEEEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]