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The map label for this gene is trpI [H]

Identifier: 209695023

GI number: 209695023

Start: 1617403

End: 1618347

Strand: Reverse

Name: trpI [H]

Synonym: VSAL_I1515

Alternate gene names: 209695023

Gene position: 1618347-1617403 (Counterclockwise)

Preceding gene: 209695029

Following gene: 209695022

Centisome position: 48.67

GC content: 42.12

Gene sequence:

>945_bases
ATGACGCCTTACCCTAATTTACCTTATTCACATCATGGCCTAAAAGCCTTTGAATCCGTGGCACGCTTGATGAGTTTCAC
CCTAGCCGCAGATGAACTTCACGTCACCCAAAGTGCCGTCAGCCGTCAAGTGAAGCAACTTGAGGATGATCTCAACACAT
CGCTTATTATTCGAAAGCACCGTTCCATAGAGCTAACATTACAAGGGATCGAGCTTTATCACGCGCTGCGAGACAATTAC
TCTGCAATTGAGTCGGTTCTATCCTCTTGGCAAGAGCCAAAACAAAAACGCATTGTCATTAAAGCCACATTAAGTTTTGC
CACTCGCGTGCTGATACCTAAAGTCAGAGAGCTCAATGAGCGTTATCCTGATCATGAAATCGTGATTATCCCTTCTTTAG
AAGAAGATGAATGTTTAGATAAGCCAGATTATGATTTACTGATCTTTCATACTCGTTTAAAAGATCATTACGACACCAAA
GCCAATATGTTTCTTCTACGAGAAGAGTTCATGGCTCCTGTTTGTGCCGCAAGTATCGCAACCGAAAGTACGAATGTAGA
CTCAATATTAACCATGCCAAGGTTGCATCCCACATTGGATCACCATGATTGGAAAATGTGGTTAGCCGATGTAACAATCC
CCCCGAACCAACAAGTCAGAAACACCAGCTTTCTTTCATTAGATTTAGCACTAAGCGCCTGCTTATCAGGACAAGGGGTA
ACCGTAACTGACCTACTGTTAATCCTTCAAGAGCTAGAAATAGGATTCTTTTATTGCCCAGAAAACGCGAAAATCCAACA
CAGTGCATGGACTTACTATGGGCACCAGCGCACTCATTCTGCCGTGATTGATGACCTTGTTGAATGGTTAAAACTCGGTT
CTGAAAACGAAATTAACGTATTGAAAAAATTAGCAAAAAAACATCATTGGCATGGTGTGGTATAA

Upstream 100 bases:

>100_bases
AATAGGCACTGTGATTAATGGCAAACTTAAGGGTTAAAAGGCTAGTGTTCAATTAATATATCCGTCACACTGACATTCTA
AAAACTCATGCGAAGAGATT

Downstream 100 bases:

>100_bases
AATGCGTCTTTTTGACGTTTTTACTCTGCAATATTGATTACATTTTAGAGAGAGATCACGTAGACTACGCGCTTATTTTC
ATGCCAACTATCAGGTGAAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: TrpBA operon transcriptional activator [H]

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MTPYPNLPYSHHGLKAFESVARLMSFTLAADELHVTQSAVSRQVKQLEDDLNTSLIIRKHRSIELTLQGIELYHALRDNY
SAIESVLSSWQEPKQKRIVIKATLSFATRVLIPKVRELNERYPDHEIVIIPSLEEDECLDKPDYDLLIFHTRLKDHYDTK
ANMFLLREEFMAPVCAASIATESTNVDSILTMPRLHPTLDHHDWKMWLADVTIPPNQQVRNTSFLSLDLALSACLSGQGV
TVTDLLLILQELEIGFFYCPENAKIQHSAWTYYGHQRTHSAVIDDLVEWLKLGSENEINVLKKLAKKHHWHGVV

Sequences:

>Translated_314_residues
MTPYPNLPYSHHGLKAFESVARLMSFTLAADELHVTQSAVSRQVKQLEDDLNTSLIIRKHRSIELTLQGIELYHALRDNY
SAIESVLSSWQEPKQKRIVIKATLSFATRVLIPKVRELNERYPDHEIVIIPSLEEDECLDKPDYDLLIFHTRLKDHYDTK
ANMFLLREEFMAPVCAASIATESTNVDSILTMPRLHPTLDHHDWKMWLADVTIPPNQQVRNTSFLSLDLALSACLSGQGV
TVTDLLLILQELEIGFFYCPENAKIQHSAWTYYGHQRTHSAVIDDLVEWLKLGSENEINVLKKLAKKHHWHGVV
>Mature_313_residues
TPYPNLPYSHHGLKAFESVARLMSFTLAADELHVTQSAVSRQVKQLEDDLNTSLIIRKHRSIELTLQGIELYHALRDNYS
AIESVLSSWQEPKQKRIVIKATLSFATRVLIPKVRELNERYPDHEIVIIPSLEEDECLDKPDYDLLIFHTRLKDHYDTKA
NMFLLREEFMAPVCAASIATESTNVDSILTMPRLHPTLDHHDWKMWLADVTIPPNQQVRNTSFLSLDLALSACLSGQGVT
VTDLLLILQELEIGFFYCPENAKIQHSAWTYYGHQRTHSAVIDDLVEWLKLGSENEINVLKKLAKKHHWHGVV

Specific function: Activates the expression of the trpBA genes encoding the two tryptophan synthase subunits. In the absence of the inducer (indoleglycerol phosphate), trpI binds upstream of the trpAB operon, overlapping its own promoter region [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=261, Percent_Identity=29.1187739463602, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786448, Length=295, Percent_Identity=25.4237288135593, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788706, Length=261, Percent_Identity=26.0536398467433, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36040; Mature: 35908

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPYPNLPYSHHGLKAFESVARLMSFTLAADELHVTQSAVSRQVKQLEDDLNTSLIIRKH
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
RSIELTLQGIELYHALRDNYSAIESVLSSWQEPKQKRIVIKATLSFATRVLIPKVRELNE
CCEEEEEHHHHHHHHHHCCHHHHHHHHHHHCCHHHCEEEEEEHHHHHHHHHHHHHHHHHH
RYPDHEIVIIPSLEEDECLDKPDYDLLIFHTRLKDHYDTKANMFLLREEFMAPVCAASIA
HCCCCEEEEEECCCCHHHCCCCCCCEEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
TESTNVDSILTMPRLHPTLDHHDWKMWLADVTIPPNQQVRNTSFLSLDLALSACLSGQGV
CCCCCCHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCEEHHHHHHHHHHCCCCC
TVTDLLLILQELEIGFFYCPENAKIQHSAWTYYGHQRTHSAVIDDLVEWLKLGSENEINV
HHHHHHHHHHHHCCCEEECCCCCEEECCEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHH
LKKLAKKHHWHGVV
HHHHHHHCCCCCCC
>Mature Secondary Structure 
TPYPNLPYSHHGLKAFESVARLMSFTLAADELHVTQSAVSRQVKQLEDDLNTSLIIRKH
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
RSIELTLQGIELYHALRDNYSAIESVLSSWQEPKQKRIVIKATLSFATRVLIPKVRELNE
CCEEEEEHHHHHHHHHHCCHHHHHHHHHHHCCHHHCEEEEEEHHHHHHHHHHHHHHHHHH
RYPDHEIVIIPSLEEDECLDKPDYDLLIFHTRLKDHYDTKANMFLLREEFMAPVCAASIA
HCCCCEEEEEECCCCHHHCCCCCCCEEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
TESTNVDSILTMPRLHPTLDHHDWKMWLADVTIPPNQQVRNTSFLSLDLALSACLSGQGV
CCCCCCHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCEEHHHHHHHHHHCCCCC
TVTDLLLILQELEIGFFYCPENAKIQHSAWTYYGHQRTHSAVIDDLVEWLKLGSENEINV
HHHHHHHHHHHHCCCEEECCCCCEEECCEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHH
LKKLAKKHHWHGVV
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2492495; 2107533; 10984043 [H]