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The map label for this gene is rbsR [H]

Identifier: 209694894

GI number: 209694894

Start: 1487925

End: 1488926

Strand: Reverse

Name: rbsR [H]

Synonym: VSAL_I1367

Alternate gene names: 209694894

Gene position: 1488926-1487925 (Counterclockwise)

Preceding gene: 209694895

Following gene: 209694892

Centisome position: 44.78

GC content: 41.32

Gene sequence:

>1002_bases
ATGGCAACGATCAAAGATGTAGCCAAACATGCCGGCGTTTCAACCTCTACTGTCAGCCATGTATTGAATAAAACGCGTTT
TGTGAGTGAAGACATTTCAGATCGCGTCGCATTTGCGGTTAAAGAACTCAACTATGCCCCATCCGCATTAGCTCGCAGCT
TAAAAGTAAATCACACGAAAACCTTTGGTATGCTCGTGACGACATCAACCAATCCTTTTTTTGGGGAGGTGGTAAAAGGC
GTAGAACGTCGTTGTTATGAGAAAGGTTATAACCTTATTTTATGTAATACCGAAGGCGATGCCGAACGTGTTCATTCAAG
CTTAGATACCCTACTTCAAAAGCGCGTTGATGGTTTGCTTCTAATGTGTACCGAAATAGAGCATCAAGTACTTGAGTTGT
TTTCTCGTTATCAAGCCGTTCCTACGGTGGTGATGGATTGGGGCTTGATTGATTTTCCAAGCGATAAAATTCAAGACAAT
TCTCATCACGGCGGTTATTTAGCGACAAAACACTTAATAGAACAAGGACACACAGACATTGGCTGTTTAACGGGTCCATT
GAATAAACTGCAAGCGCAACAGCGTTTAAGTGGCTTTGTACAAGCAATGGAAGAATCAGGATTAACCATCAATAAAAACT
GGATCGCCTCAGGAAATTTTGAATGTGAAGGCGGTGAAAGTGCATTTAACGCTCTTCATGCAAGAGGGGCATTACCAACC
GCACTCTTTGTGTGTAATGACATGATGGCAATGGGCATGATTAATTGCGCGAGTAAAAAAGGGATTTCGGTGCCTAATGA
TATTTCAGTGGTTGGTTACGATGATATTAATATGGCGAAATACATCACACCATCGTTAACCACAATTCATCAGCCAAAGC
ATCGTTTAGGCCAACAAGCCGTTGATTCATTGTTAGAAAAGATTGCCACTAAGTCTGATACTAATCGTGTCATTCAACTG
GAACCGACATTAATGATTCGTGACAGTGTTAAAACGATTTAA

Upstream 100 bases:

>100_bases
AGAGCGTTTTCTATTAGAGCATTGAGCATTACTCTCTATTTCAGTGCCTTTGACTGCCATTCATAACGCAGTCAAAGACA
CAAACAAAGGATTGGATTTT

Downstream 100 bases:

>100_bases
TATCAAGCCGCTAGCTACATTAAAAAATAGAGTTTCTATCCATGTAGAAACTCTATTAATATTTTTCACGAACCGCAATA
TATCGCGCAAACTTAGGAAT

Product: ribose operon repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MATIKDVAKHAGVSTSTVSHVLNKTRFVSEDISDRVAFAVKELNYAPSALARSLKVNHTKTFGMLVTTSTNPFFGEVVKG
VERRCYEKGYNLILCNTEGDAERVHSSLDTLLQKRVDGLLLMCTEIEHQVLELFSRYQAVPTVVMDWGLIDFPSDKIQDN
SHHGGYLATKHLIEQGHTDIGCLTGPLNKLQAQQRLSGFVQAMEESGLTINKNWIASGNFECEGGESAFNALHARGALPT
ALFVCNDMMAMGMINCASKKGISVPNDISVVGYDDINMAKYITPSLTTIHQPKHRLGQQAVDSLLEKIATKSDTNRVIQL
EPTLMIRDSVKTI

Sequences:

>Translated_333_residues
MATIKDVAKHAGVSTSTVSHVLNKTRFVSEDISDRVAFAVKELNYAPSALARSLKVNHTKTFGMLVTTSTNPFFGEVVKG
VERRCYEKGYNLILCNTEGDAERVHSSLDTLLQKRVDGLLLMCTEIEHQVLELFSRYQAVPTVVMDWGLIDFPSDKIQDN
SHHGGYLATKHLIEQGHTDIGCLTGPLNKLQAQQRLSGFVQAMEESGLTINKNWIASGNFECEGGESAFNALHARGALPT
ALFVCNDMMAMGMINCASKKGISVPNDISVVGYDDINMAKYITPSLTTIHQPKHRLGQQAVDSLLEKIATKSDTNRVIQL
EPTLMIRDSVKTI
>Mature_332_residues
ATIKDVAKHAGVSTSTVSHVLNKTRFVSEDISDRVAFAVKELNYAPSALARSLKVNHTKTFGMLVTTSTNPFFGEVVKGV
ERRCYEKGYNLILCNTEGDAERVHSSLDTLLQKRVDGLLLMCTEIEHQVLELFSRYQAVPTVVMDWGLIDFPSDKIQDNS
HHGGYLATKHLIEQGHTDIGCLTGPLNKLQAQQRLSGFVQAMEESGLTINKNWIASGNFECEGGESAFNALHARGALPTA
LFVCNDMMAMGMINCASKKGISVPNDISVVGYDDINMAKYITPSLTTIHQPKHRLGQQAVDSLLEKIATKSDTNRVIQLE
PTLMIRDSVKTI

Specific function: Transcriptional repressor for the ribose rbsDACBK operon. RbsR binds to a region of perfect dyad symmetry spanning the rbs operon transcriptional start site. The affinity for the rbs operator is reduced by addition of ribose, consistent with ribose being

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=329, Percent_Identity=55.3191489361702, Blast_Score=380, Evalue=1e-106,
Organism=Escherichia coli, GI1787948, Length=332, Percent_Identity=48.1927710843374, Blast_Score=340, Evalue=8e-95,
Organism=Escherichia coli, GI1790369, Length=337, Percent_Identity=31.4540059347181, Blast_Score=195, Evalue=4e-51,
Organism=Escherichia coli, GI1789202, Length=333, Percent_Identity=35.4354354354354, Blast_Score=184, Evalue=5e-48,
Organism=Escherichia coli, GI1788474, Length=333, Percent_Identity=31.2312312312312, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1789068, Length=307, Percent_Identity=31.5960912052117, Blast_Score=167, Evalue=6e-43,
Organism=Escherichia coli, GI1787580, Length=321, Percent_Identity=29.9065420560748, Blast_Score=164, Evalue=8e-42,
Organism=Escherichia coli, GI1786540, Length=312, Percent_Identity=32.0512820512821, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1790715, Length=327, Percent_Identity=25.6880733944954, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787906, Length=315, Percent_Identity=24.4444444444444, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI48994940, Length=327, Percent_Identity=26.2996941896024, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789456, Length=328, Percent_Identity=27.1341463414634, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786268, Length=311, Percent_Identity=21.2218649517685, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790689, Length=321, Percent_Identity=22.7414330218069, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36626; Mature: 36495

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVAKHAGVSTSTVSHVLNKTRFVSEDISDRVAFAVKELNYAPSALARSLKVNHTK
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEE
TFGMLVTTSTNPFFGEVVKGVERRCYEKGYNLILCNTEGDAERVHSSLDTLLQKRVDGLL
EEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCE
LMCTEIEHQVLELFSRYQAVPTVVMDWGLIDFPSDKIQDNSHHGGYLATKHLIEQGHTDI
EHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHCCCCCCCCCHHHHHHHHHHCCCCC
GCLTGPLNKLQAQQRLSGFVQAMEESGLTINKNWIASGNFECEGGESAFNALHARGALPT
HHHCCCHHHHHHHHHHHHHHHHHHHCCCEEECCEEECCCEECCCCHHHHHHHHHCCCCHH
ALFVCNDMMAMGMINCASKKGISVPNDISVVGYDDINMAKYITPSLTTIHQPKHRLGQQA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHCCCCHHHHCCHHHHHHHHH
VDSLLEKIATKSDTNRVIQLEPTLMIRDSVKTI
HHHHHHHHHCCCCCCCEEEECCCEEECCCCCCC
>Mature Secondary Structure 
ATIKDVAKHAGVSTSTVSHVLNKTRFVSEDISDRVAFAVKELNYAPSALARSLKVNHTK
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEE
TFGMLVTTSTNPFFGEVVKGVERRCYEKGYNLILCNTEGDAERVHSSLDTLLQKRVDGLL
EEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCE
LMCTEIEHQVLELFSRYQAVPTVVMDWGLIDFPSDKIQDNSHHGGYLATKHLIEQGHTDI
EHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHCCCCCCCCCHHHHHHHHHHCCCCC
GCLTGPLNKLQAQQRLSGFVQAMEESGLTINKNWIASGNFECEGGESAFNALHARGALPT
HHHCCCHHHHHHHHHHHHHHHHHHHCCCEEECCEEECCCEECCCCHHHHHHHHHCCCCHH
ALFVCNDMMAMGMINCASKKGISVPNDISVVGYDDINMAKYITPSLTTIHQPKHRLGQQA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHCCCCHHHHCCHHHHHHHHH
VDSLLEKIATKSDTNRVIQLEPTLMIRDSVKTI
HHHHHHHHHCCCCCCCEEEECCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]